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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of gynecology and obstetrics 230 (1981), S. 293-297 
    ISSN: 1432-0711
    Keywords: Schwangerschaftsspezifisches Protein SP-1 ; Tubargravidität ; Enzym-Immunoassay ; Pregnancy specific protein SP-1 ; Tubal pregnancy ; Enzyme immunoassay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Serum pregnancy specific protein (SP-1) levels were measured preoperatively in 26 women with suspected ectopic pregnancy. We used an enzyme immunoassay with a sensitivity of 2 ng/ml. SP-1 was detected in all 23 patients with a tubal pregnancy, but not in three nonpregnant women with ovarian cysts. Tests for urinary HCG with a sensitivity of 1,000 IU/l were negative in seven of 19 patients with an ectopic pregnancy.
    Notes: Zusammenfassung Mit Hilfe des Enzym-Immunoassays Enzygnost-SP-1 wurde die Serumkonzentration von SP-1 bei 26 Frauen, die unter der Verdachtsdiagnose Tubargravidität operiert wurden, praeoperativ bestimmt. Bei allen 23 Patientinnen mit verifizierter Tubargravidität war SP-1 im Serum nachweisbar, während bei drei nicht graviden Frauen mit Ovarialcysten die Werte von SP-1 unter 2 ng/ml lagen. Routinemäßig durchgeführte Schwangerschaftsteste im Harn hingegen waren in sieben von 19 Fällen mit Tubargravidität negativ. Offenbar kann durch die Messung der SP-1 Serumkonzentration bei Fällen mit Verdacht auf Extrauteringravidität die Diagnostik entscheidend verbessert werden.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0983
    Keywords: Yeast ; Sequence ; Amino-Acid Permease ; Carboxypeptidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have analysed two new genes, YBR1007 and YBR1015, discovered during the systematic sequencing of chromosome II of S. cerevisiae. YBR1007 shows strong similarities to amino-acid permeases, in particular the high-affinity proline permeases of S. cerevisiae and A. nidulans. The number and position of the predicted membrane-spanning domains suggest a conserved structure for these proteins, with 12 trans-membrane domains. YBR1015 shows strong similarities to serine carboxypeptidases; all three residues of the “catalytic triad” typical of this family of enzymes are conserved in the YBR1015 protein. In a preliminary functional analysis we have created a null allele of the YBR1015 gene, and shown that it is not essential for cellular viability.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1612-4766
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Laboratory bioassay of the two fungiMetarbizium anisopliae (Metschnikoff) Sorokin andVerticillium indicum (Petch) Gams was carried out under 26±1 C° and 75±2% R. H. This is the first study on the efficiency of both mycopathogens against the termiteCryptotermes brevis (Walker). Using the highest concentration ofM. anisopliae (43.5 × 105 V.S/ml), percentages of mortality after 4 weeks from treatment of the termiteC. brevis fed on filter paper or wood were 100 and 93.33, respectively. On the same time, the highest concentration of the fungiV. indicum (34.3 × 105 V.S/ml) resulted in the mortality percentages of 83.33 and 70, respectively, fed on filter paper and wood. In general, the data indicated more susceptibility of the termiteC. brevis to the entomopathogenic fungusM. anisopliae than to the fungusV. indicum. M. anisopliae could be considered as a promising potential mycopathogen against the termiteC. brevis. This study, presenting new approach of the alternative dry wood termite control, might be applied in the future.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1617-4623
    Keywords: Antisense RNA ; YBR1012/YBR136w/MEC1/ESR1 ; Saccharomyces cerevisiae ; Flow cytometry ; Cell cycle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract YBR1012 (YBR136w) is an essential gene from Saccharomyces cerevisiae identified during the systematic sequencing of part of the right arm of chromosome II. We previously constructed a conditional allele of YBR1012 based on antisense RNA, by inserting a small fragment of this gene downstream from the inducible UASGAL10-CYC1 promoter. Several other antisense RNA constructions have since been made and their activity tested. The response of the system appears to be very delicate, as the presence or absence of 13 nucleotides of polylinker in the 300 nucleotide antisense transcript can dramatically modify its effectiveness. The most effective antisense RNA construction was used in flow cytometry studies to investigate the role of ybr1012p. The results show that during the antisense RNA block some 80% of the cells are arrested with their DNA unreplicated, suggesting that Ybr1012p is needed for progression through G1 or early S phase.
    Type of Medium: Electronic Resource
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