ISSN:
1573-4986
Keywords:
Trichoderma viride
;
β-glucosidase
;
transglycosylation
;
two-dimensional NMR spectroscopy
;
oligosaccharide synthesis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract A new β-glucosidase was partially purified from Trichoderma viride cellulase. This β-glucosidase catalyzed a transglycosylation reaction of cellobiose to give β-D-Glc-(1→6)-β-D-Glc-(1→4)-D-Glc (1, yield: 18.8%) and β-D-Glc-(1→6)-β-D-Glc-(1→6)-β-D-Glc-(1→4)-D-Glc (2, 3.7%), regioselectively. Furthermore, the enzyme regioselectively converted laminaribiose and gentiobiose into β-D-Glc-(1→6)-β-D-Glc-(1→3)-D-Glc (3, 15.3%) and β-D-Glc-(1→6)-β-D-Glc-(1→6)-D-Glc (4, 20.2%), respectively. The structures (1–4) of the products were determined by 1H and 13C NMR spectroscopies. This high regio- and stereoselectively of the β-glucosidase could be applied for oligosaccharide synthesis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1007034728857
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