ISSN:
1573-6881
Schlagwort(e):
Membrane potential
;
reconstituted plasma membrane vesicles
;
oxonol VI fluorescence
;
yeast H+-ATPase
;
Schizosaccharomyces pombe
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
,
Chemie und Pharmazie
,
Physik
Notizen:
Abstract A model of membrane potential-dependent distribution of oxonol VI to estimate the electrical potential difference Δψ across Schizosaccharomyces pombe plasma membrane vesicles (PMV) has been developed. Δψ was generated by the H+-ATPase reconstituted in the PMV. The model treatment was necessary since the usual calibration of the dye fluorescence changes by diffusion potentials (K+ + valinomycin) failed. The model allows for fitting of fluorescence changes at different vesicle and dye concentrations, yielding Δψ in ATP-energized PMV of 80 mV. The described model treatment to estimate Δψ may be applicable for other reconstituted membrane systems.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1023/A:1022439203461
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