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  • 1
    Electronic Resource
    Electronic Resource
    Tungstate can substitute for molybdate in sustaining growth of Methanobacterium thermoautotrophicum (1994)
    Springer
    Archives of microbiology 161 (1994), S. 220-228 
    Details
    ISSN: 1432-072X
    Keywords: Formylmethanofuran dehydrogenase ; Tungsten enzymes ; Molybdopterin dinucleotides ; Methanogenesis ; Archaea ; Archaebacteria ; Methanobacterium thermoautotrophicum ; Methanobacterium wolfei
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Methanobacterium thermoautotrophicum (strain Marburg) was found to grow on media supplemented with tungstate rather than with molybdate. The Archaeon then synthesized a tungsten iron-sulfur isoenzyme of formylmethanofuran dehydrogenase. The isoenzyme was purified to apparent homogeneity and shown to be composed of four different subunits of apparent molecular masses 65 kDa, 53 kDa, 31 kDa, and 15 kDa and to contain per mol 0.4 mol tungsten, 〈0.05 mol molybdenum, 8 mol non-heme iron, 8 mol acid-labile sulfur and molybdopterin guanine dinucleotide. Its molecular and catalytic properties were significantly different from those of the molybdenum isoenzyme characterized previously. The two isoenzymes also differed in their metal specificity: the active molybdenum isoenzyme was only synthesized when molybdenum was available during growth whereas the active tungsten isoenzyme was also generated during growth of the cells on molybdate medium. Under the latter conditions the tungsten isoenzyme was synthesized containing molybdenum rather than tungsten.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00248696
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Tungstate does not support synthesis of active formylmethanofuran dehydrogenase in Methanosarcina barkeri (1994)
    Springer
    Archives of microbiology 161 (1994), S. 528-530 
    Details
    ISSN: 1432-072X
    Keywords: Tungsten enzymes ; Molybdenum enzymes ; Formylmethanofuran dehydrogenase ; Methanogenic Archaea ; Methanosarcina barkeri ; Methanobacterium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cell extracts of Methanosarcina barkeri grown on methanol in media supplemented with molybdate exhibited a specific activity of formylmethanofuran dehydrogenase of approximately 1 U (1 μmol/min)/mg protein. When the growth medium was supplemented with tungstate rather than with molybdate, the specific activity was only 0.04 U/mg. Despite this reduction in specific activity growth on methanol was not inhibited. An inhibition of both growth and synthesis of active formylmethanofuran dehydrogenase was observed, however, when H2 and CO2 were the energy substrates. The results indicate that, in contrast to Methanobacterium wolfei and Methanobacterium thermoautotrophicum, M. barkeri possesses only a molybdenum containing formylmethanofuran dehydrogenase and not in addition a tungsten isoenzyme.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307775
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Tungstate can substitute for molybdate in sustaining growth of Methanobacterium thermoautotrophicum (1994)
    Springer
    Archives of microbiology 161 (1994), S. 220-228 
    Details
    ISSN: 1432-072X
    Keywords: Key words: Formylmethanofuran dehydrogenase – Tungsten enzymes – Molybdopterin dinucleotides – Methanogenesis – Archaea – Archaebacteria –Methanobacterium thermoautotrophicum–Methanobacterium wolfei
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Methanobacterium thermoautotrophicum (strain Marburg) was found to grow on media supplemented with tungstate rather than with molybdate. The Archaeon then synthesized a tungsten iron-sulfur isoenzyme of formylmethanofuran dehydrogenase. The isoenzyme was purified to apparent homogeneity and shown to be composed of four different subunits of apparent molecular masses 65 kDa, 53 kDa, 31 kDa, and 15 kDa and to contain per mol 0.4 mol tungsten, 〈0.05 mol molybdenum, 8 mol non-heme iron, 8 mol acid-labile sulfur and molybdopterin guanine dinucleotide. Its molecular and catalytic properties were significantly different from those of the molybdenum isoenzyme characterized previously. The two isoenzymes also differed in their metal specificity: the active molybdenum isoenzyme was only synthesized when molybdenum was available during growth whereas the active tungsten isoenzyme was also generated during growth of the cells on molybdate medium. Under the latter conditions the tungsten isoenzyme was synthesized containing molybdenum rather than tungsten.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00248696
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    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Internal Glutamine and Glutamate Pools in Klebsiella pneumoniae Grown Under Different Conditions of Nitrogen Availability (2000)
    Springer
    Current microbiology 41 (2000), S. 357-362 
    Details
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Internal pool sizes of glutamine and glutamate in Klebsiella pneumoniae grown under nitrogen limitation or nitrogen sufficiency were measured to study the signal transduction of external nitrogen limitation. K. pneumoniae cells were grown in an anaerobic, ammonium-limited chemostat culture. At a growth rate of 0.217 h−1, the steady state ammonium concentration in the culture was 55 μm, correlating with repression of the nitrogen fixation (nif) genes. At growth rates below 0.138 h−1, the ammonium concentration in the culture dropped below 0.5 μm and the nif genes became derepressed. During the transition from nitrogen sufficiency to nitrogen limitation, the internal glutamine pool in K. pneumoniae decreased by a factor of approximately 6. The glutamate pool, however, remained stable. Similarly, in anaerobic batch cultures with different limiting nitrogen sources, the glutamine pool generally decreased by a factor of 7 to 9 when nif gene derepression was achieved. All the limiting nitrogen sources used resulted in decreased growth rates compared with growth under nitrogen excess, suggesting an inverse relationship between glutamine pool size and doubling time. These studies indicate that K. pneumoniae perceives external nitrogen limitation as internal glutamine limitation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002840010149
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    Paper (German National Licenses)
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  • 5
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    Epibacterial community patterns on marine macroalgae are host-specific but temporally variable (2011)
    Wiley
    In:  Environmental Microbiology, 13 (3). pp. 655-665.
    Details
    Publication Date: 2019-09-23
    Description: Marine macroalgae are constantly exposed to epibacterial colonizers. The epiphytic bacterial patterns and their temporal and spatial variability on host algae are poorly understood. To investigate the interaction between marine macroalgae and epiphytic bacteria, this study tested if the composition of epibacterial communities on different macroalgae was specific and persisted under varying biotic and abiotic environmental conditions over a 2-year observation time frame. Epibacterial communities on the co-occurring macroalgae Fucus vesiculosus, Gracilaria vermiculophylla and Ulva intestinalis were repeatedly sampled in summer and winter of 2007 and 2008. The epibacterial community composition was analysed by denaturing gradient gel electrophoresis (DGGE) and 16S rRNA gene libraries. Epibacterial community profiles did not only differ significantly at each sampling interval among algal species, but also showed consistent seasonal differences on each algal species at a bacterial phylum level. These compositional patterns re-occurred at the same season of two consecutive years. Within replicates of the same algal species, the composition of bacterial phyla was subject to shifts at the bacterial species level, both within the same season but at different years and between different seasons. However, 7–16% of sequences were identified as species specific to the host alga. These findings demonstrate that marine macroalgae harbour species-specific and temporally adapted epiphytic bacterial biofilms on their surfaces. Since several algal host-specific bacteria were highly similar to other bacteria known to either avoid subsequent colonization by eukaryotic larvae or to exhibit potent antibacterial activities, algal host-specific bacterial associations are expected to play an important role for marine macroalgae.
    Type: Article , PeerReviewed
    Format: text
    DOI: 10.1111/j.1462-2920.2010.02371.x
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    OceanRep: Article in a Scientific Journal - peer-reviewed
  • 6
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    Cultivable microbiota associated with Aurelia aurita and Mnemiopsis leidyi (2020)
    Wiley
    Details
    Publication Date: 2023-02-08
    Description: The associated microbiota of marine invertebrates plays an important role to the host in relation to fitness, health, and homeostasis. Cooperative and competitive interactions between bacteria, due to release of, for example, antibacterial substances and quorum sensing (QS)/quorum quenching (QQ) molecules, ultimately affect the establishment and dynamics of the associated microbial community. Aiming to address interspecies competition of cultivable microbes associated with emerging model species of the basal animal phyla Cnidaria (Aurelia aurita) and Ctenophora (Mnemiopsis leidyi), we performed a classical isolation approach. Overall, 84 bacteria were isolated from A. aurita medusae and polyps, 64 bacteria from M. leidyi, and 83 bacteria from ambient seawater, followed by taxonomically classification by 16S rRNA gene analysis. The results show that A. aurita and M. leidyi harbor a cultivable core microbiome consisting of typical marine ubiquitous bacteria also found in the ambient seawater. However, several bacteria were restricted to one host suggesting host-specific microbial community patterns. Interbacterial interactions were assessed by (a) a growth inhibition assay and (b) QS interference screening assay. Out of 231 isolates, 4 bacterial isolates inhibited growth of 17 isolates on agar plates. Moreover, 121 of the 231 isolates showed QS-interfering activities. They interfered with the acyl-homoserine lactone (AHL)-based communication, of which 21 showed simultaneous interference with autoinducer 2. Overall, this study provides insights into the cultivable part of the microbiota associated with two environmentally important marine non-model organisms and into interbacterial interactions, which are most likely considerably involved in shaping a healthy and resilient microbiota.
    Type: Article , PeerReviewed
    Format: text
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    OceanRep: Article in a Scientific Journal - peer-reviewed
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