Description: The study site Zamostye (51.18481 °N, 35.27988 °E, 135 m a.s.l.) is situated in the south-west of the Mid-Russian Upland in the south of the East European Plain, in the center of the East-European forest-steppe ecotone. The knowledge of the history of the formation of the ecotone can help sustainability of the land management as well as show its response to climatic changes, however the area is considerably understudied. In order to close this gap, we conducted a study of the vegetation history based on a multi-proxy analysis of a sediment core obtained in the village Zamostye (Kursk region, Russia). The study site represents a wetland overgrown by Phragmites and surrounded by alder trees and grasslands with ruderal vegetation. The sediment core was obtained with a Russian corer from an overgrown oxbow lake near the river Sudzha in July 2013 and consists of two sister cores - “Zamostye I” (0-176 cm from surface) and “Zamostye II” (25-250 cm). The two cores were taken close to each other with overlapping segments and combined based on LOI results to obtain a composite core Zamostye. 11 radiocarbon ages were acquired to ensure a robust age-depth model. The core was studied for pollen, non-pollen palynomorphs, loss-on-ignition, macroremains and charcoal. Due to the lack of the archives like lakes and mires in the area, this record covering the last 14,800 years is unique for the region and provides a continuous vegetation history from the Late Glacial to the present. In total, 66 samples in 1 to 4-cm resolution were obtained for palynological, NPP and micro-charcoal analysis. Laboratory treatment followed the standard processing technique by Faegri and Iversen (1989) included 10 % HCl, 48 % HF left overnight and acetolysis for 3 minutes. The samples were then sieved through 200 µm metallic mesh and 6 µm nylon mesh using an ultrasound bath for less than 1 minute. Before the chemical treatment, one Lycopodium spores' tablet (batch number 1031, 20848 ± 1546 spores per tablet) was added to each sample to calculate palynomorphs concentration and influx (Stockmarr, 1971). Macro-charcoal analysis was performed in 2-cm resolution. The samples were treated following Stevenson and Haberle (2005): 10 % KOH, 6 % H2O2 and sieved through 125 µm metallic mesh. The charcoal was identified under 100× magnification. Four morphological types were identified using Jensen et al. (2007). We analyzed 14 samples for macroremains to track the changes in local vegetation and sediment composition. The entire 1-cm thick section of the core was sieved through 250 µm and studied under 40× to 100× magnification. We used Cappers et al. (2006) and Göttingen University collection to identify the seeds of the plants.

Description: Omelchenki study site is located at the southern border of the East-European forest-steppe ecotone next to the village of Omelchenki in the Zmiiv district of the Kharkiv region, Ukraine (49.63969 N, 36.41677 E, 88 m a.s.l.). The sediment core was obtained from a peatbog ~3 km south and west from the Siverskyi Donets River channel, in May 2013 (2013-05-05) and consists of two sister cores – Omelchenki I (0-150 cm) and Omelchenki II (25-175 cm). The two cores were taken close to each other with overlapping segments with a Russian corer. Based on the LOI results we combined the first 25 cm of the Omelchenki I core with the main Omelchenki II core to obtain a continuous record of Omelchenki. This work was aimed to reconstruct the southern tree line dynamics and vegetation changes in Eastern Europe during the Holocene due to climate change, fires and human activities. It contains unique for the East-European forest-steppe palynological data covering the last ~9,800 years. To determine the chronology, 9 AMS radiocarbon dates were carried out in the AMS Radiocarbon Laboratories in Poznan, Poland and the Institute of Physics of the University of Erlangen Nuremberg, Germany. The radiocarbon ages were calibrated and the age-depth model was built using the R package 'bacon' based on Bayesian statistics (Blaauw and Christen, 2011; doi:10.1214/11-BA618) with the IntCal20 calibration curve for the Northern Hemisphere (Reimer et al., 2020; doi:10.1017/RDC.2020.41).

Description: In order to reconstruct regional vegetation changes and local conditions during the fen-bog transition in the Borsteler Moor (northwestern Germany), a sediment core covering the period between 7.1 and 4.5 cal kyrs BP was palynologically in vestigated. The pollen diagram demonstrates the dominance of oak forests and a gradual replacement of trees by raised bog vegetation with the wetter conditions in the Late Atlantic. At ~ 6 cal kyrs BP, the non-pollen palynomorphs (NPP) demonstrate the succession from mesotrophic conditions, clearly indicated by a number of fungal spore types, to oligotrophic conditions, indicated by Sphagnum spores, Bryophytomyces sphagni, and testate amoebae Amphitrema, Assulina and Arcella, etc. Four relatively dry phases during the transition from fen to bog are clearly indicated by the dominance of Calluna and associated fungi as well as by the increase of microcharcoal. Several new NPP types are described and known NPP types are identified. All NPP are discussed in the context of their palaeoecological indicator values.