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  • BIOPRESS LIMITED  (2)
  • PUBLIC LIBRARY SCIENCE  (2)
  • 1
    Publication Date: 2014-07-01
    Description: Current sampling of genomic sequence data from eukaryotes is relatively poor, biased, and inadequate to address important questions about their biology, evolution, and ecology; this Community Page describes a resource of 700 transcriptomes from marine microbial eukaryotes to help understand their role in the world's oceans.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , isiRev
    Format: application/pdf
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  • 2
    Publication Date: 2018-09-11
    Description: The recently established diatom genus Shionodiscus is characterized as possessing a labiate process on the valve face and strutted processes with long internal and reduced, or no, external extensions. Many Thalassiosira species with these features were transferred to this new genus, as well as some with short internal, strutted process extensions. Examination of samples from the Argentine continental shelf and slope waters, and from the Beagle Channel, revealed the presence of a small centric diatom that formed an extensive bloom in slope waters during spring 2005 and 2006. Analysis by light and electron microscopy revealed high similarity to specimens previously referred as Thalassiosira bioculata var. raripora. This name was never effectively published; nevertheless, the name has been adopted and observations referring to it have been reported on several occasions. Here we trace the usage of the (nomenclaturally invalid) name Thalassiosira bioculata var. raripora in the literature, and formally describe the species as Shionodiscus gaarderae, based on our observations and comparison with previous reports. A morphological comparison of this and similar taxa is also provided. In addition, we offer an emended description of S. bioculatus var. bioculatus from Ostenfeld material sampled from the "Färöer Island Naströ Fjord Plankton (16.9.1902)”, held in the Hustedt collection. Finally, we propose the transfer of Thalassiosira rosulata to Shionodiscus.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , isiRev
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  • 3
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    BIOPRESS LIMITED
    In:  EPIC3Diatom Research, BIOPRESS LIMITED, 30(1), ISSN: 0269-249X
    Publication Date: 2015-10-27
    Description: Vanhoeffenus antarcticus was described by Heinrich Heiden from a net sample taken from 385 m depth by the Gauss expedition (First German Antarctic Expedition) while frozen in pack ice near the Antarctic continent in the austral summer of 1902–3. Valves of this peculiar diatom have only ever been observed in preparations from this locality and only using light microscopy, but an electron microscopy study of this taxon has become possible now that some surviving material from the Gauss expedition has been recovered. Our scanning electron microscopy observations show the presence of a radially arranged system of marginal chambers, connected to hollow radial rays and continuing through unusually long processes to the inside of the cell. Another unique characteristic of this taxon is a marginal ring of pillars bearing hooks rising from the valve mantle–valve face boundary, previously interpreted (based on light microscopy) as a ring of T-shaped spines. However, despite the improved understanding of its valve structure, the systematic position of this taxon and its biology remain enigmatic.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , isiRev
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  • 4
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    PUBLIC LIBRARY SCIENCE
    In:  EPIC3PLoS ONE, PUBLIC LIBRARY SCIENCE, 7(11), pp. e48624, ISSN: 1932-6203
    Publication Date: 2019-07-16
    Description: Marine dinoflagellates (alveolata) are microalgae of which some cause harmful algal blooms and produce a broad variety of most likely polyketide synthesis derived phycotoxins. Recently, novel polyketide synthesase (PKS) transcripts have been described from the Florida red tide dinoflagellate Karenia brevis (gymnodiniales) which are evolutionarily related to Type I PKS but were apparently expressed as monofunctional proteins, a feature typical of Type II PKS. Here, we investigated expression units of PKS I-like sequences in Alexandrium ostenfeldii (gonyaulacales) and Heterocapsa triquetra (peridiniales) at the transcript and protein level. The five full length transcripts we obtained were all characterized by polyadenylation, a 3′ UTR and the Dinoflagellate specific spliced leader sequence at the 5′end. Each of the five transcripts encoded a single ketoacylsynthase (KS) domain showing high similarity to K. brevis KS sequences. The monofunctional structure was also confirmed using dinoflagellate specific KS antibodies in Western Blots. In a maximum likelihood phylogenetic analysis of KS domains from diverse PKSs, dinoflagellate KSs formed a clade placed well within the protist Type I PKS clade between apicomplexa, haptophytes and chlorophytes. These findings indicate that the atypical PKS I structure, i.e., expression as putative monofunctional units, might be a dinoflagellate specific feature. In addition, the sequenced transcripts harbored a previously unknown, apparently dinoflagellate specific conserved N-terminal domain. We discuss the implications of this novel region with regard to the putative monofunctional organization of Type I PKS in dinoflagellates.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , isiRev
    Format: application/pdf
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