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  • 1
    Electronic Resource
    Electronic Resource
    Tungstate can substitute for molybdate in sustaining growth of Methanobacterium thermoautotrophicum (1994)
    Springer
    Archives of microbiology 161 (1994), S. 220-228 
    Details
    ISSN: 1432-072X
    Keywords: Formylmethanofuran dehydrogenase ; Tungsten enzymes ; Molybdopterin dinucleotides ; Methanogenesis ; Archaea ; Archaebacteria ; Methanobacterium thermoautotrophicum ; Methanobacterium wolfei
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Methanobacterium thermoautotrophicum (strain Marburg) was found to grow on media supplemented with tungstate rather than with molybdate. The Archaeon then synthesized a tungsten iron-sulfur isoenzyme of formylmethanofuran dehydrogenase. The isoenzyme was purified to apparent homogeneity and shown to be composed of four different subunits of apparent molecular masses 65 kDa, 53 kDa, 31 kDa, and 15 kDa and to contain per mol 0.4 mol tungsten, 〈0.05 mol molybdenum, 8 mol non-heme iron, 8 mol acid-labile sulfur and molybdopterin guanine dinucleotide. Its molecular and catalytic properties were significantly different from those of the molybdenum isoenzyme characterized previously. The two isoenzymes also differed in their metal specificity: the active molybdenum isoenzyme was only synthesized when molybdenum was available during growth whereas the active tungsten isoenzyme was also generated during growth of the cells on molybdate medium. Under the latter conditions the tungsten isoenzyme was synthesized containing molybdenum rather than tungsten.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00248696
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Tungstate does not support synthesis of active formylmethanofuran dehydrogenase in Methanosarcina barkeri (1994)
    Springer
    Archives of microbiology 161 (1994), S. 528-530 
    Details
    ISSN: 1432-072X
    Keywords: Tungsten enzymes ; Molybdenum enzymes ; Formylmethanofuran dehydrogenase ; Methanogenic Archaea ; Methanosarcina barkeri ; Methanobacterium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cell extracts of Methanosarcina barkeri grown on methanol in media supplemented with molybdate exhibited a specific activity of formylmethanofuran dehydrogenase of approximately 1 U (1 μmol/min)/mg protein. When the growth medium was supplemented with tungstate rather than with molybdate, the specific activity was only 0.04 U/mg. Despite this reduction in specific activity growth on methanol was not inhibited. An inhibition of both growth and synthesis of active formylmethanofuran dehydrogenase was observed, however, when H2 and CO2 were the energy substrates. The results indicate that, in contrast to Methanobacterium wolfei and Methanobacterium thermoautotrophicum, M. barkeri possesses only a molybdenum containing formylmethanofuran dehydrogenase and not in addition a tungsten isoenzyme.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307775
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Tungstate can substitute for molybdate in sustaining growth of Methanobacterium thermoautotrophicum (1994)
    Springer
    Archives of microbiology 161 (1994), S. 220-228 
    Details
    ISSN: 1432-072X
    Keywords: Key words: Formylmethanofuran dehydrogenase – Tungsten enzymes – Molybdopterin dinucleotides – Methanogenesis – Archaea – Archaebacteria –Methanobacterium thermoautotrophicum–Methanobacterium wolfei
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Methanobacterium thermoautotrophicum (strain Marburg) was found to grow on media supplemented with tungstate rather than with molybdate. The Archaeon then synthesized a tungsten iron-sulfur isoenzyme of formylmethanofuran dehydrogenase. The isoenzyme was purified to apparent homogeneity and shown to be composed of four different subunits of apparent molecular masses 65 kDa, 53 kDa, 31 kDa, and 15 kDa and to contain per mol 0.4 mol tungsten, 〈0.05 mol molybdenum, 8 mol non-heme iron, 8 mol acid-labile sulfur and molybdopterin guanine dinucleotide. Its molecular and catalytic properties were significantly different from those of the molybdenum isoenzyme characterized previously. The two isoenzymes also differed in their metal specificity: the active molybdenum isoenzyme was only synthesized when molybdenum was available during growth whereas the active tungsten isoenzyme was also generated during growth of the cells on molybdate medium. Under the latter conditions the tungsten isoenzyme was synthesized containing molybdenum rather than tungsten.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00248696
    Location Call Number Limitation Availability
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    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Internal Glutamine and Glutamate Pools in Klebsiella pneumoniae Grown Under Different Conditions of Nitrogen Availability (2000)
    Springer
    Current microbiology 41 (2000), S. 357-362 
    Details
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Internal pool sizes of glutamine and glutamate in Klebsiella pneumoniae grown under nitrogen limitation or nitrogen sufficiency were measured to study the signal transduction of external nitrogen limitation. K. pneumoniae cells were grown in an anaerobic, ammonium-limited chemostat culture. At a growth rate of 0.217 h−1, the steady state ammonium concentration in the culture was 55 μm, correlating with repression of the nitrogen fixation (nif) genes. At growth rates below 0.138 h−1, the ammonium concentration in the culture dropped below 0.5 μm and the nif genes became derepressed. During the transition from nitrogen sufficiency to nitrogen limitation, the internal glutamine pool in K. pneumoniae decreased by a factor of approximately 6. The glutamate pool, however, remained stable. Similarly, in anaerobic batch cultures with different limiting nitrogen sources, the glutamine pool generally decreased by a factor of 7 to 9 when nif gene derepression was achieved. All the limiting nitrogen sources used resulted in decreased growth rates compared with growth under nitrogen excess, suggesting an inverse relationship between glutamine pool size and doubling time. These studies indicate that K. pneumoniae perceives external nitrogen limitation as internal glutamine limitation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002840010149
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    Paper (German National Licenses)
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  • 5
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    Phosphate solubilization and multiple plant growth promoting properties of rhizobacteria isolated from chickpea (Cicer aeritinum L.) producing areas of Ethiopia (2016)
    Academic Journals
    In:  African Journal of Biotechnology, 15 (35). pp. 1899-1912.
    Details
    Publication Date: 2019-09-23
    Description: Chickpea is one of the major legume crops widely grown in Ethiopia. The low availability of phosphorus in soil is among the stresses that constrain the production of this crop in the country. However, there are rhizobacteria capable of solubilizing insoluble forms of phosphorus in soil and make it available to the plant. Thus, this study was aimed at isolation and characterization of phosphate solubilizing bacteria from chickpea rhizosphere. Fifty phosphate solubilizing bacterial strains were isolated from the soil samples, characterized biochemically and identified by 16S rDNA sequences analysis. The results indicate the presence of genera Acinetobacter, Bacillus, Brevibacillus, Burkholderia, Empedobacter, Enterobacter, Pseudomonas, Ralstonia, Sphingomonas and Stenotrophomonas. Phosphate solubilizing efficiencies of the strains were analyzed using different insoluble phosphorus sources and the results show that most isolates released a substantial amount of soluble phosphate from tricalcium phosphate, rock phosphate and bone meal. Screening for multiple plant growth promoting attributes showed that 44 and 18% of them were capable of producing indole acetic acid and inhibiting the growth of Fusarium oxysporum under in vitro conditions, respectively. A direct impact of several strains (Bacillus flexus (PSBC17), Pseudomonas fluorescence (PSBC33), Enterobacter sp. (PSBC35), Enterobacter sakazaki (PSBC79) and Enterobacter sp. (PSBC81)) on the growth of chickpea in pot culture has been demonstrated by the increase in the number of root nodules, shoot dry matter, nitrogen and phosphorus concentration of shoot. Based on the results, we conclude that chickpea rhizosphere harbor phosphate solubilizing bacteria which are diverse in taxonomy and phosphate solubilizing efficiencies. Thus, consecutive studies should focus on field studies on those strains due to their potentially high importance for the phosphorus nutrition of crops in this area and in this context for the improvement of the sustainability of crop production in the country
    Type: Article , PeerReviewed
    Format: text
    DOI: 10.5897/AJB2015.15172
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    OceanRep: Article in a Scientific Journal - peer-reviewed
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