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  • Cell & Developmental Biology  (1)
  • Gl. lacrimalis (extraorbitalis)  (1)
  • Succinic dehydrogenase  (1)
  • 1965-1969  (2)
Document type
Keywords
Publisher
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Anatomy and embryology 128 (1969), S. 141-162 
    ISSN: 1432-0568
    Keywords: Salivary glands ; Succinic dehydrogenase ; Alkaline phosphatase ; Gl. lacrimalis (extraorbitalis) ; Salivary reabsorption
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Bei der Ratte wurden die Ausführungsgangsysteme der drei großen Speicheldrüsen und der Gl. lacrimalis extraorbitalis hinsichtlich des Gehaltes ihrer Epithelien an Succinodehydrogenase sowie in bezug auf die Art und Dichte ihrer Kapillarisierung untersucht. Die Drüsengefäße (Capillaren) stellten wir durch die Reaktion der Endothelzellen auf alkalische Phosphatase dar. Bei den Speichelgangsystemen fand sich jeweils der höchste Gehalt an Succinodehydrogenase in den Epithelien der intralobulären Ganganteile. Generell nahm mit zunehmender Ganggröße die Reaktionsstärke ab. Das Gangsystem der Gl. retrolingualis fiel durch einen besonders hohen Enzymgehalt auf. Bei der Gl. lacrimalis extraorbialis war — beschränkt auf die extraglandulären Ganganteile — nur eine schwach positive bis negative Reaktion zu erzielen. Bei den Speicheldrüsen zeigten sich an allen Anteilen der Gangsysteme Capillarnetze; bei der Tränendrüse waren solche nur an den außerhalb des Drüsenkörpers liegenden Gangabschnitten zu beobachten. So wie der Gehalt an Succinodehydrogenase nahm auch die Kapillarisationsdichte an den Ausführungsgangsystemen der untersuchten Speicheldrüsen im Verlauf der Gänge nach peripher ab. Die unterschiedliche Dichte der Capillarnetze an den Gangsystemen der drei großen Rattenspeicheldrüsen läßt auf unterschiedlich starke Austauschvorgänge schließen. Die auf Mikropunktionsuntersuchungen beruhende Annahme, daß der Ductus submandibularis noch zu einer Veränderung des ihn passierenden Sekretes beiträgt, wird morphologisch durch den Nachweis enggestellter Capillarnetze und histochemisch durch eine stark positive Reaktion der Gangepithelien auf Succinodehydrogenase gestützt. Für den Ductus retrolingualis gelten ähnliche Überlegungen, obwohl noch keine Mikropunktionsuntersuchungen vorliegen. Beim Ductus parotideus dagegen bestätigen der weitmaschige Capillarplexus und der geringe Gehalt der Gangepithelien an Succinodehydrogenase die Auffassung früherer Untersucher, daß der Parotisgang (und seine extraglandulären Zuflüsse) keinen wesentlichen Einfluß mehr auf die Zusammensetzung des Endspeichels ausübt.
    Notes: Summary The duct systems of the great salivary glands and the gl. lacrimalis extraorbitalis of the rat have been examined with respect to their activity of succinic dehydrogenase, as well as with respect to the appearance and density of their capillarisation. The blood vessels (capillaries) have been demonstrated by means of the reaction of their endothelium to alkaline phosphatase. The highest activity of succinic dehydrogenase has been found in the epithelial cells of the intralobular ducts (of the salivary glands). Generally decreasing activity was observed in the ducts with enlargening diameter. In the ducts of the gl. retrolingualis, the enzyme reaction is particularly strong. The duct system of the gl. lacrimalis extraorbitalis has a weak positive to negative reaction in its outer-glandular part. On all parts of the salivary duct systems there is a capillary network. In the lacrimal gland, however only the outerglandular part of the duct system is characterized by the capillary network. The capillary network of the salivary duct systems are seen to become wider as the diameter of the ducts becomes greater. The different density of the networks surrounding the salivary duct systems probably indicate exchange processes of varying intensity. The theory that the ductus submandibularis may alter the composition of the passing saliva is supported by the finding of a dense capillary network and high activity of succinic dehydrogenase in the ducts. The same features are found in the ductus retrolingualis, although here the theory of alteration of saliva by the ducts is not yet proved by micropuncture experiments. The assumption of preceding investiations that the function of the ductus parotideus does almost completely consist in transporting the saliva, is supported by a wide-spread capillary network and by a minute activity of succinic dehydrogenase of the duct cells.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A morphological and histochemical investigation was conducted on the pronephros and mesonephros of the mouse embryo from 8.5 through 16.5 days. The pronephros appeared between days 8.5 and 9.5 as a thickening of the somatic layer of the intermediate cell mass. It consisted of three small clusters of cells on either side of the midline dorsally between the somite and the coelom, at the level of somites 8 and 9. The mesonephros arose during day 9 and persisted until day 16. In the male the anterior three tubules were incorporated into the testis at 15.5-16.5 days. The mesonephros consisted of approximately 11 tubules located between somites 10-17. The tubules possessed lumina and connected with the Wolffian duct. Indications of internal and external glomeruli were noted on day 11. The Wolffian duct reached the cloaca at ten days.Strong alkaline phosphatase activity was noted in the differentiating tubules. Cytoplasmic and luminal enzyme activity was observed between 9.3 and 12.5 days indicating possible function at this time. Acid phosphatase was demonstrable in the tubules and duct only on day 11. Ribonucleic acid was observed in the nuclei and cytoplasm of the mesodermal cells as they differentiated into tubules and duct. A decrease in RNA was noted after differentiation was complete. Periodic acid-Schiff material (diastase-stable) was localized in the basement membrane of the tubule and duct cells. A faint positive reaction was also found at the luminal border of the tubules. The strongest reaction was noted in the luminal border at 11.5-12.5 days. Those tubules being incorporated into the genital system in the male were also PAS positive. Morphological and histochemical evidence suggested that the mouse mesonephros, though quasi vestigial, may function for a short time.
    Type of Medium: Electronic Resource
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