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  • Clostridium pasteurianum  (3)
  • Acetate and CO2 assimilation  (1)
  • Chemolithothrophic growth  (1)
  • 1975-1979  (5)
Document type
Keywords
Publisher
Years
  • 1975-1979  (5)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Nickel requirement for carbon monoxide dehydrogenase formation in Clostridium pasteurianum (1979)
    Springer
    Archives of microbiology 122 (1979), S. 117-120 
    Details
    ISSN: 1432-072X
    Keywords: Nickel ; Carbon monoxide oxidation ; Clostridium pasteurianum ; Nitrilotriacetate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Formation of carbon monoxide dehydrogenase in growing Clostridium pasteurianum was found to be dependent on trace nickel present as contaminant in the growth medium. The evidence is: i) Synthesis of the enzyme was increased, when NiCl2 (0.1 μM) was added to the medium; ii) Synthesis of the enzyme was almost completely inhibited when the cells were grown in the presence of nitrilotriacetate (0.1 mM) or of other chelating agents, which inhibited the uptake of trace nickel from the medium; iii) Inhibition of enzyme synthesis by the chelators could be specifically overcome by supplementing the medium with nickel (1μM).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00408054
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Growth yields and growth rates of Desulfovibrio vulgaris (Marburg) growing on hydrogen plus sulfate and hydrogen plus thiosulfate as the sole energy sources (1978)
    Springer
    Archives of microbiology 117 (1978), S. 209-214 
    Details
    ISSN: 1432-072X
    Keywords: Desulfovibrio ; Chemolithothrophic growth ; H2 oxidation ; Sulfate reduction ; Thiosulfate reduction ; Growth rates ; Growth yields ; Maintenance coefficients ; Y ATP max
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Desulfovibrio vulgaris (Marburg) was grown on H2 plus sulfate and H2 plus thiosulfate as the sole energy sources and acetate plus CO2 as the sole carbon sources. Conditions are described under which the bacteria grew exponentially. Specific growth rates (μ) and molar growth yields (Y) at different pH were determined. μ and Y were found to be strongly dependent on the pH. Highest growth rates and molar growth yields were observed for growth on H2 plus sulfate at pH 6.5 (μ=0.15h-1; Y SO 4 2- =8.3g·mol-1) and for growth on H2 plus thiosulfate at pH 6.8 (μ=0.21h-1; Y S 2O 3 2 =16.9g·mol-1). The growth yields were found to increase with increasing growth rates: plots of 1/Y versus 1/μ were linear. Via extrapolation to infinite growth rates a Y SO4 2- /max of 12.2g·mol-1 and a YS2O 3 2- /max of 33.5g·mol-1 was obtained. The growth yield data are interpred to indicate that dissimilatory sulfate reduction to sulfide is associated with a net synthesis of 1 mol of ATP and that near to 3 mol of ATP are formed during dissimilatory sulfite reduction to sulfide.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00402310
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Ferredoxin degradation in growing Clostridium pasteurianum during periods of iron deprivation (1979)
    Springer
    Archives of microbiology 120 (1979), S. 73-76 
    Details
    ISSN: 1432-072X
    Keywords: Ferredoxin ; Clostridium pasteurianum ; Pyruvate synthase ; Iron-sulfur proteins ; Iron metabolism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Clostridium pasteurianum was grown in batch cultures on media with an initial iron concentration of 10 μM. The uptake of iron and the synthesis of ferredoxin was followed. All the iron present in the medium was taken up by the cells before 50% of the final cell density was attained. The bacteria then continued to grow in the complete absence of exogenous iron. Ferredoxin was synthesized during growth until the exogenous iron concentration dropped below 1 μM. During growth in the absence of iron ferredoxin was degraded with the result that at the end of growth the cells did not contain ferredoxin. The specific activity of the iron sulfur protein, pyruvate synthase (E.C. 1.2.7.1), remained constant during growth of C. pasteurianum in the absence of exogenous iron. This finding suggests that ferredoxin was used as an endogenous source of iron for the synthesis of essential iron proteins during periods of iron deprivation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00413277
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  • 4
    Electronic Resource
    Electronic Resource
    The active species of “CO2” utilized in ferredoxin-linked carboxylation reactions (1975)
    Springer
    Archives of microbiology 104 (1975), S. 237-240 
    Details
    ISSN: 1432-072X
    Keywords: CO2-Fixation ; Carboxylation Reactions ; Pyruvate Synthase ; Pyruvate: Ferredoxin Oxidoreductase ; Ferredoxin ; Thiamine Pyrophosphate ; Acetyl CoA ; Clostridium pasteurianum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The active species of “CO2” , i.e. CO2 or HCO 3 − −(H2CO3) utilized by enzymes catalyzing ferredoxin-linked carboxylation reactions was determined. The enzyme investigated was pyruvate synthase from Clostridium pasteurianum (EC 1.2.7.1; Pyruvate: ferredoxin oxidoreductase). Data were obtained which were compatible with those expected if CO2 is the active species. The dissociation constant (K S) of the enzyme-CO2 complex was measured. At pH 7.2 K Sfor CO2 of pyruvate synthase was found to be approximately 5 mM.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00447330
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  • 5
    Electronic Resource
    Electronic Resource
    Acetate and carbon dioxide assimilation by Desulfovibrio vulgaris (Marburg), growing on hydrogen and sulfate as sole energy source (1979)
    Springer
    Archives of microbiology 123 (1979), S. 301-305 
    Details
    ISSN: 1432-072X
    Keywords: Desulfovibrio ; Chemolithotrophic growth ; Acetate and CO2 assimilation ; Amino acid synthesis ; (R)-Citrate sythase ; Pentose phosphates synthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Desulfovibrio vulgaris (Marburg) was grown on hydrogen plus sulfate as sole energy source and acetate plus CO2 as the sole carbon sources. The incorporation of U-14C acetate into alanine, aspartate, glutamate, and ribose was studied. The labelling data show that alanine is synthesized from one acetate (C-2 + C-3) and one CO2 (C-1), aspartate from one acetate (C-2 + C-3) and two CO2 (C-1 + C-4), glutamate from two acetate (C-1−C-4) and one CO2 (C-5), and ribose from 1.8 acetate and 1.4 CO2. These findings indicate that in Desulfovibrio vulgaris (Marburg) pyruvate is formed via reductive carboxylation of acetyl-CoA, oxaloacetate via carboxylation of pyruvate or phosphoenol pyruvate, and α-ketoglutarate from oxaloacetate plus acetyl-CoA via citrate and isocitrate. Since C-5 of glutamate is derived from CO2, citrate must have been formed via a (R)-citrate synthase rather than a(S)-citrate synthase. The synthesis of ribose from 1.8 mol of acetate and 1.4 mol of CO2 excludes the operation of the Calvin cycle in this chemolithotrophically growing bacterium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00406665
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    Paper (German National Licenses)
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