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  • ATP synthesis  (1)
  • Acetate and CO2 assimilation  (1)
  • Cell carbon synthesis  (1)
  • 1975-1979  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Isolation and characterization of Desulfovibrio growing on hydrogen plus sulfate as the sole energy source (1978)
    Springer
    Archives of microbiology 116 (1978), S. 41-49 
    Details
    ISSN: 1432-072X
    Keywords: Desulfovibrio ; Chemolithotrophic growth ; H2-Oxidation ; Sulfate-reduction ; Growth yields ; Cell carbon synthesis ; Acetate assimilation ; Desulfoviridin ; Cytochrome c3
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two sulfate reducing bacteria (Madison and Marburg strains) that grew on H2 plus sulfate in a mineral salts medium that contained acetate and CO2 as sole carbon source were isolated from diverse environments. During growth in this medium 4.2 mol of H2 were consumed per mol of sulfate reduced to sulfide. Acetate was required for biosynthetic purposes only. Approximately 70% of the cell carbon synthesized was derived from acetate and 30% from CO2. Acetate was not involved in dissimilatory sulfate reduction. Growth of the bacteria on H2 plus sulfate was linear rather than exponential, and a doubling time at the beginning of linear growth of approximately 3 h was observed. The optimal growth temperature was found to be near 35° C. Cultures could be grown up to a density of 500 mg cells (dry weight) per liter. Growth yield studies demonstrated that between 4 and 5 g of cells (dry weight) were formed per mol of sulfate reduced to sulfide. The chemolithotrophically growing sulfate reducing isolates were identified as Desulfovibrio species by being obligately anaerobic, gram negative, non spore forming vibrios that contained desulfoviridin and cytochrome c3 (350–450 nmol/g protein). The organisms were found to be monopolarly and monotrichously flagellated. The abilities of the two strains to grow on electron donors other than H2 and to use electron acceptors other than sulfate differed considerably. The DNA base composition of the Madison and Marburg strains were 60 and 63.5 mol % GC, respectively. The taxonomic status of the strains was discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00408732
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Function of fumarate reductase in methanogenic bacteria (Methanobacterium) (1978)
    Springer
    Archives of microbiology 119 (1978), S. 215-218 
    Details
    ISSN: 1432-072X
    Keywords: Methanobacterium thermoautotrophicum ; ATP synthesis ; Funtarate reductase ; Menaquinone ; Cytochromeb ; Succinate dehydrogenase ; α-Ketoglutarate synthesis ; Succinate incorporation ; Fumarate incorporation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Methanogenic bacteria contain high activities of fumarate reductase. An interesting hypothesis has recently been advanced that this enzyme, in cooperation with a succinate dehydrogenase, functions in a fumarate-succinate cycle for ATP synthesis. This hypothesis was tested by determining whether [2, 3-3H] succinate loses3H when taken up by growing cells.Methanobacterium thermoautotrophicum was grown on H2 plus CO2 in the presence of [U-14C, 2,3-3H] succinate. The double labelled dicarboxylic acid was found to be incorporated into cell material with the loss of only 30% of tritium. Neither was3H released into H2O in significant amounts. This finding excludes a catabolic oxidation of succinate to fumarate in the growing cells and thus the operation of a fumaratesuccinate cycle. It is shown that the function of fumarate reductase inM. thermoautotrophicum is to provide the cells with succinate for the synthesis of α-ketoglutarate, an intermediate in glutamate, arginine and proline synthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00964276
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Acetate and carbon dioxide assimilation by Desulfovibrio vulgaris (Marburg), growing on hydrogen and sulfate as sole energy source (1979)
    Springer
    Archives of microbiology 123 (1979), S. 301-305 
    Details
    ISSN: 1432-072X
    Keywords: Desulfovibrio ; Chemolithotrophic growth ; Acetate and CO2 assimilation ; Amino acid synthesis ; (R)-Citrate sythase ; Pentose phosphates synthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Desulfovibrio vulgaris (Marburg) was grown on hydrogen plus sulfate as sole energy source and acetate plus CO2 as the sole carbon sources. The incorporation of U-14C acetate into alanine, aspartate, glutamate, and ribose was studied. The labelling data show that alanine is synthesized from one acetate (C-2 + C-3) and one CO2 (C-1), aspartate from one acetate (C-2 + C-3) and two CO2 (C-1 + C-4), glutamate from two acetate (C-1−C-4) and one CO2 (C-5), and ribose from 1.8 acetate and 1.4 CO2. These findings indicate that in Desulfovibrio vulgaris (Marburg) pyruvate is formed via reductive carboxylation of acetyl-CoA, oxaloacetate via carboxylation of pyruvate or phosphoenol pyruvate, and α-ketoglutarate from oxaloacetate plus acetyl-CoA via citrate and isocitrate. Since C-5 of glutamate is derived from CO2, citrate must have been formed via a (R)-citrate synthase rather than a(S)-citrate synthase. The synthesis of ribose from 1.8 mol of acetate and 1.4 mol of CO2 excludes the operation of the Calvin cycle in this chemolithotrophically growing bacterium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00406665
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    Paper (German National Licenses)
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