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  • 1985-1989  (15)
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Year
  • 1
    Electronic Resource
    Electronic Resource
    Protection of bacteria against toxicity of phenol by immobilization in calcium alginate (1989)
    Springer
    Applied microbiology and biotechnology 31 (1989), S. 383-389 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The antibacterial activity of phenol was determined by measuring inhibition of exponentially growing free and immobilized cells of Escherichia coli, Pseudomonas putida and Staphylococcus aureus. Immobilization of microorganisms in calcium alginate beads reduced the growth inhibition caused by bacteriostatic concentrations of phenol. The increase in phenol tolerance occurred at different culture conditions and growth rates of the cells. The strength of the effect, however, was found to correlate with the formation of colonies in the gel matrix. Dissolution of gel beads led to a substantial loss of the protection against phenol of immobilized-grown cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00257609
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Identification and production of Δ9-cis-1,18-octadecenedioic acid by Candida tropicalis (1989)
    Springer
    Applied microbiology and biotechnology 32 (1989), S. 371-371 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00184991
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Bioconversion of elaidic acid to Δ9-trans-1,18-octadecenedioic acid by Candida tropicalis (1988)
    Springer
    Applied microbiology and biotechnology 29 (1988), S. 305-309 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The bioconversion of elaidic acid to Δ9 acid by the mutant S76 of Candida tropicalis was studied. It was found that the mutant could convert elaidic acid to Δ9 acid through the ω-oxidation of the terminal methyl group. The molecular formula and geometric configuration of the product were determined by different analytical methods in the absence of an authentic standard. The results demonstrated that no change in the geometric configuration occured during the conversion of the unsaturated monoic acid to α,ω-dioic acid. The production of this dioic acid reached 10 mg/ml in medium for 36 h with a conversion rate of 40%. Degradation pathways of this dioic acid are basically similar to the dioic acid derived from oleic acid by the same strain except for a small amount of heptanedioic acid which was observed in the medium. The IR spectrum, mass-spectrum, 1H-NMR and 13C-satellite spectra of this compound are presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00265811
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Chlortetracycline production with immobilized Streptomyces aureofaciens (1987)
    Springer
    Applied microbiology and biotechnology 26 (1987), S. 333-337 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Spores of Streptomyces aureofaciens ATCC 10762 were immobilized in calcium alginate, and the chlortetracycline production was examined. The influence of alginate concentration and spore concentration on the antibiotic production was investigated. By optimizing the nutrient composition of the production medium, the chlortetracycline yields of batch-fermented immobilized microorganisms could be increased fourfold. Chlortetracycline production by free mycelia was 40% of that with immobilized microorganisms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00256664
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Identification and production of Δ9-cis-1, 18-octadecenedioic acid by Candida tropicalis (1989)
    Springer
    Applied microbiology and biotechnology 30 (1989), S. 327-331 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The production of Δ9-cis-1, 18-octadecenedioic acid from different substrates by the mutant S76 of Candida tropicalis was studied. It was found that the mutant could convert oleyl alcohol, oleic acid, and methyl oleate to Δ9-cis-1, 18-octadecenedioic acid through either the diterminal oxidation of the alcohol or the ω-oxidation of the terminal methyl group of the mono acid. The geometric configuration of the product was identified by different analytical methods. The results demonstrated that no change in the geometric configuration of molecular structure happened during the bioconversion of such unsaturated substrates to Δ9-dioic acid. To enhance the production of this dioic acid in the medium, calcium carbonate was added as a “trapping agent”. The IR spectrum, 1H NMR and 13C-satellite spectra of this compound were presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00296619
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  • 6
    Electronic Resource
    Electronic Resource
    Continuous penicillin production by Penicillium chrysogenum immobilized in calcium alginate beads (1987)
    Springer
    Applied microbiology and biotechnology 26 (1987), S. 215-218 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A high penicillin-producing Penicillium chrysogenum strain immobilized in calcium alginate beads was used for continuous penicillin fermentation in a bubble column and in a conical bubble fermentor. The fermentation was limited by the growth rate, dilution rates and the stability of the alginate beads. The immobilized cells lost their ability to produce penicillin in the bubble column after 48 h from beginning of the continuous fermentation. In the conical bubble fermentor the immobilized cells remained active for more than 7 days. This bioreactor ensured a good distribution of nutrients and oxygen as well as a higher mechanical stability of the alginate beads.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00286311
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Exhaust gas purification using immobilised monocultures (biocatalysts) (1987)
    Springer
    Applied microbiology and biotechnology 26 (1987), S. 579-587 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The paper is concerned with the purification of exhaust gases using biocatalysts in a trickle bed reactor. Substance specific strains (monocultures) which were, for example, immobilised on activated carbon served as biocatalyst. Technically important solvents and substances such as aldehydes, methyl ethyl ketone and ethyl acetate were used as pollutants. Their concentration was about 5–40 ppm in the exhaust gas to be purified. The experiments show that with suitable bacterial, strains space velocities of about k *=1500 h-1 can be obtained at a conversion of 90%. The mass transfer through the liquid film around the activated carbon grains seems to be rate determining.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00253036
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    Paper (German National Licenses)
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  • 8
    Electronic Resource
    Electronic Resource
    Influence of growth behaviour and physiology of alginate-entrapped microorganisms on the oxygen consumption (1988)
    Springer
    Applied microbiology and biotechnology 29 (1988), S. 554-559 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The ability of alginate-entrapped microorganisms to supply oxygen was determined with regard to physiology and growth behavior of the cells. Oxygen diffusion through an alginate film containing different concentrations of Pseudomonas putida or Saccharomyces cerevisiae was measured. Oxygen diffusion decreased when the cell loading increased. Dependent on the physiological behavior of these organisms the course of the oxygen concentration under the gel film is quite different. In further experiments an “Effectiveness-Factor” of oxygen uptake of alginate beads with Saccharomyces cerevisiae or Aspergillus niger was determined in relation to the growth behavior of the organisms. The effectiveness factor is always higher when the biomass is concentrated in the outer region of the gel beads as if the microorganisms are distributed homogeneously in the alginate. Considering these results it is not possible to make a general statement on the ability of microorganisms in alginate to supply oxygen. The physiology and the growth behavior of the immobilized organisms have to be considered in any case.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00260984
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    Paper (German National Licenses)
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  • 9
    Electronic Resource
    Electronic Resource
    Semicontinuous penicillin production by two Penicillium chrysogenum strains immobilized in calcium alginate beads (1987)
    Springer
    Applied microbiology and biotechnology 26 (1987), S. 211-214 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The growth rates of immobilized Penicillium chrysogenum strains are important in their application to semicontinuous penicillin production. Immobilized P. chrysogenum strains produced about 10–15% less biomass but about 1–2 times more penicillin than free suspended mycelia. In a chemically defined medium an industrial P. chrysogenum strain, S1, produced about 10–12 times more penicillin than strain ATCC 12690. In a complex medium the immobilized P. chrysogenum S1 produced about 12% penicillin more than in shaken cultures. In bubble column fermentations, penicillin production was 163% higher in the complex medium than in the chemically defined medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00286310
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    Paper (German National Licenses)
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  • 10
    Electronic Resource
    Electronic Resource
    Chlortetracycline production with immobilized Streptomyces aureofaciens (1987)
    Springer
    Applied microbiology and biotechnology 26 (1987), S. 338-341 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The semicontinuous production of chlortetracycline by immobilized cells of Streptomyces aureofaciens ATCC 10762 was compared with that of free cells. Immobilized cells transferred repeatedly to a new production medium, showed a fourfold increase in the half life time of antibiotic production. In an air bubble column a high chlortetracycline productivity was obtained with a high aeration rate. A semicontinuous production of chlortetracycline by immobilized S. aureofaciens could be improved by varying the fermentation conditions. For continuous chlortetracycline production by immobilized cells, no improvement was detected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00256665
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    Paper (German National Licenses)
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