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  • 1985-1989  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Isolierte menschliche Magenschleimhautzellen — Untersuchungen physiologischer und pharmakologischer Regulationsmechanismen (1986)
    Springer
    Journal of molecular medicine 64 (1986), S. 15-22 
    Details
    ISSN: 1432-1440
    Keywords: Isolated gastric mucosal cells (man) ; 14C-aminopyrine uptake ; Intrinsic factor ; Adenylate cyclase ; Histamine ; Prostaglandins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Cells were isolated by use of collagenase, EDTA and pronase form human gastric mucosa obtained at peptic ulcer surgery (n=61) or at Whipple's operations (n=6). Enriched parietal cell fractions were prepared by isopycnic centrifugation with Percoll. H+ production, intracellular instrinsic factor and histamine content were maximal in the low density fraction containing 75% parietal cells and — among other nonparietal cell types — mast cells. H+ production, intrinsic factor secretion and adenylate cyclase-activity responded to histamine stimulation in a concentration dependent manner. Response was blocked by histamine H2 receptor antagonists (rantidine, famotidine). Dibutyryl cAMP and the phosphodiesterase inhibitor IMX were the most powerful stimuli whereas carbachol, hexoprenaline and pentagastrin were less effective. Prostaglandin E2 and 6-keto-PGF2α occurred in the highest concentrations in the low density cell fraction. PG production increased linearly for 15 min and seemed to be influenced by the intracellular calcium level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01721576
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of glucagon on adenylate cyclase activity and acid production of isolated human parietal cells (1986)
    Springer
    Journal of molecular medicine 64 (1986), S. 746-749 
    Details
    ISSN: 1432-1440
    Keywords: Glucagon ; Adenylate cyclase ; 14C-aminopyrine ; Human parietal cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The direct effect of glucagon on human parietal cell function in vitro was tested by measuring adenylate cyclase (AC) activity and H+ production in homogenates of human gastric mucosa obtained during surgery or at biopsy. Cells isolated from mucosa obtained during surgery showed an increase in AC with histamine and glucagon. In parietal cell enriched fractions (75%) glucagon and histamine stimulated AC much more effectively than in parietal cell depleted fractions (15% and 7%). In contrast, glucagon did not affect basal or histamine stimulated14C amino pyrine uptake. In homogenates of mucosal biopsy specimens 2×10−7 mol/l glucagon enhanced AC activity by 76% (corpus) and 20% (antrum). In the same homogenates 10−4 mol/l histamine caused a stimulation by 161% (corpus) and 38% (antrum). In fundic biopsy specimens glucagon displayed a biphasic concentration response curve with an increase at 10−10 mol/l (46% above basal AC activity) and a maximum at 2×10−7 mol/l (97%). Histamine elicited the maximal response (192%) at 10−3 mol/l. Increasing histamine and glucagon concentrations caused additive stimulation of AC. Ranitidine did not change AC in response to glucagon but abolished the effect of histamine. Data suggest that the glucagon action is mediated by separate (glucagon?) receptors. As H+ production was not affected by glucagon, the coexistence of two AC systems in the human parietal cell is postulated: One that is activated via histamine H2-receptors and which stimulates H+ production; another that is activated by glucagon and is directed towards other, possibly metabolic effects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01734342
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    Paper (German National Licenses)
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