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  • Lactococci  (1)
  • glacial maximum  (1)
  • 1995-1999  (2)
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  • 1995-1999  (2)
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  • 1
    ISSN: 1432-072X
    Keywords: Key words Biosynthesis of extracellular ; polysaccharide ; Lipid-linked intermediate ; Lactococci ; Polysaccharide ; Undecaprenol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Lactococcus lactis subspecies cremoris SBT 0495 produces the phosphopolysaccharide viilian, which consists of the repeating unit β-d-glucosyl-(1→4)-(α-l-rhamnosyl-(1→2))-(α-d-galactose-1-phosphoryl-(→3)-β-galactosyl-(1→4)-β-d-glucose. A lipid extract was prepared from cells in the late exponential phase of growth and was hydrolyzed by hydrochloric acid under mild conditions to split lipid-linked intermediates in the extract into lipid and sugar moieties. Both moieties were purified by chromatographic techniques and were characterized to identify intermediates of the viilian biosynthetic pathway. A polyisoprenoid isolated from the chloroform-soluble fraction of the hydrolyzed lipid extract was identified by mass spectrometry as undecaprenol. Saccharides isolated from the water-soluble fraction of the hydrolyzed lipid extract by anion-exchange chromatography, were characterized by glycosidic linkage analysis to discriminate sugar moieties of intermediates of viilian biosynthesis from compounds liberated from cell wall components. Some oligosaccharide analogues contain a glycerol residue, suggesting that these are fragments of glycosylglycerides and/or lipoteichoic acid. Three fragments were identified to be glucose, galactosyl-(1→4)-glucose, and rhamnosyl-(1→2)-galactosyl-(1→4)-glucose, which are in agreement with the structure of the repeating unit of viilian. These saccharides most likely represent the first three steps of the sequential assembly of the repeating unit of the undecaprenol assembly.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of oceanography 55 (1999), S. 483-492 
    ISSN: 1573-868X
    Keywords: Paleoceanography ; box model ; Japan Sea ; glacial maximum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract The Japan Sea has experienced drastic changes in the last 60 ka: the surface water was colder than the present value by five degrees and extremely freshened (∼24 ppt) in the last glacial maximum (∼15 ka), and then it contained Oyashio water for a few thousand years. It is an open question whether the inflow-outflow pattern was entirely reversed, opposite to the present exchange with an inflow through Tsushima Strait and an outflow through Tsugaru Strait. A box model is employed with two boxes representing the northern and the southern half domains in the upper (300-m-thick) layer. The model is driven by atmospheric forcing and inflow through Tsushima Strait and/or Tsugaru Strait. Here, the net transport through Tsushima to Tsugaru is given in the model. A baroclinic component is added to the net transport through each strait. It is the baroclinic components that allow the upper and the lower portions to flow to the opposite directions in the straits, and hence a reversal flow becomes possible against the net transport, under the condition of an extremely freshened Japan Sea. The fresh surface layer in 18∼14 ka is attributable to a near-shutoff of the inflow due to the low sea level. Shortly after the near-shutoff, the baroclinic transport through Tsugaru Strait yields intrusion of the Oyashio water into the Japan Sea. Thus, it is implied that Oyashio water existed in the Japan Sea a few thousand years after the reopening of Tsugaru Strait, even though the net transport was one-way, similar to the present state.
    Type of Medium: Electronic Resource
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