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  • 1
    Electronic Resource
    Electronic Resource
    Alarm pheromone perception in honey bees is decreased by smoke (Hymenoptera: Apidae) (1995)
    Springer
    Journal of insect behavior 8 (1995), S. 11-18 
    Details
    ISSN: 1572-8889
    Keywords: honey bee ; Apis mellifera ; alarm pheromone ; electroantennograph
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The application of smoke to honey bee(Apis mellifera) antennae reduced the subsequent electroantennograph response of the antennae to honey bee alarm pheromones, isopentyl acetate, and 2-heptanone. This effect was reversible, and the responsiveness of antennae gradually returned to that of controls within 10–20 min. A similar effect occurred with a floral odor, phenylacetaldehyde, suggesting that smoke interferes with olfaction generally, rather than specifically with honey bee alarm pheromones. A reduction in peripheral sensitivity appears to be one component of the mechanism by which smoke reduces nest defense behavior of honey bees.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01990966
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Characterization of angiotensin receptors on human skin fibroblasts (1997)
    Springer
    Journal of molecular medicine 75 (1997), S. 217-222 
    Details
    ISSN: 1432-1440
    Keywords: Key words Angiotensin II ; Human skin fibroblasts ; Hypertension ; Arteriosclerosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Angiotensin II is involved in blood pressure regulation, cell growth and angioneogenesis. The angiotensin receptors which mediate the intracellular effects of angiotensin II are expressed in numerous tissues and cell types. We studied the expression of angiotensin II receptors in cultured human skin fibroblasts derived from a skin biopsy. Angiotensin II binding characteristics were analyzed by radioligand binding assays. The DNA synthesis was assessed by [H]thymidine incorporation assays. Intracellular calcium concentrations were measured by fura-2 spectrofluorometry, and mRNA expression levels were analyzed by northern blot technology. Two distinct angiotensin receptors were detectable on human skin fibroblasts: the AT1 receptor with K d=1.0± 0.7 nmol/l and B max=17.9±0.9 fmol/mg protein, and an angiotensin(1–7) binding site with K d=26±6.6 nmol/l and B max=80.4±3.5 fmol/mg protein, as shown by competition binding assays using selective angiotensin II receptor antagonists and the heptapeptide angiotensin(1–7). The angiotensin AT1 receptor mRNA was substantially expressed in human skin fibroblasts and was subjected to homologous downregulation. In human skin fibroblasts angiotensin II caused a profound increase in intracellular calcium which was blocked by angiotensin AT1 receptor antagonists such as Exp-3174. Furthermore, both angiotensin II and angiotensin(1–7) led to increased DNA synthesis in human skin fibroblasts. In conclusion, cultured human skin fibroblasts express angiotensin AT1 receptors and a putatively new angiotensin receptor activated by angiotensin(1–7), both coupled to signaling pathways involved in DNA synthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001090050106
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Influence of Age on Antennal Response of Male Honey Bees, Apis mellifera, to Queen Mandibular Pheromone and Alarm Pheromone Component (1997)
    Springer
    Journal of chemical ecology 23 (1997), S. 1867-1880 
    Details
    ISSN: 1573-1561
    Keywords: Apis mellifera honey bee ; drone bees ; electroantennogram ; queen pheromone ; alarm pheromone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Using a computer-controlled pheromone-puff delivery system with signal averaging, we investigated the change with age of the electroantennogram (EAG) response of drone (male) honey bees to queen mandibular pheromone (QMP) and an alarm pheromone component, isopentyl acetate (IPA). Drone antennae were significantly more sensitive than worker antennae to QMP, but there was no significant difference in response to IPA. Response to QMP was dose dependent and decreased gradually with the age of the drone. Drone response to IPA did not diminish significantly with age. Drones do not mature sexually or make their first flights until about 1 week old, so the ontogeny of their sensory responsiveness to queen pheromones does not reflect that of their behavior. It is unknown if drones exhibit any behavioral response to IPA. The signal-averaging system we describe could be used to improve resolution of low signal-to-noise ratio signals in other EAG studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/B:JOEC.0000006456.90528.94
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    Paper (German National Licenses)
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