ISSN:
1573-2568
Keywords:
HELICOBACTER PYLORI
;
LOW-MOLECULAR-WEIGHT ANTIGEN
;
33-35 K ANTIGEN
;
CagA
;
VacA
;
PATHOGENESIS
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Early studies suggested that two Helicobacterpylori proteins, CagA and VacA, were virulence factors.Support for that hypothesis has been undermined bygeographic differences in prevalence of these antigens. To identify other possible putative virulencefactors by establishing a relationship between antigensand different H. pylori diseases, two commercialavailable immunoblot assay kits, HelicoBlot 2.0(Genelabs Diagnostics, Singapore) and RIDA BlotHelicobacter (R-Biopharm GmbH, Darmstadt, Germany), wereused to investigate the prevalence of various specificantigen seropositivity in 80 H. pylori-infected Japanese (20 each with gastritis, duodenal ulcer,gastric ulcer, or gastric cancer). The production ofinterleukin-8 (IL-8) in biopsy specimens was alsomeasured by enzyme-linked immunosorbent assay (ELISA).Both assays had 100% sensitivity; specificity was90% for HB2.0 and 80% for RIDA-BH. With the exception ofthe 33-35 K antigen, there was no relationship betweenantigens, endoscopic diagnoses, histological findings, or mucosal IL-8 levels. The 33-35 Kantigen was present in 97.5% (39 of 40) patients withgastric or duodenal ulcer compared to 70% (14 of 20)those with chronic gastritis (P 〈 0.006). The mean IL-8 levels in the corpus wassignificantly higher in those with antibody to the 33-35K antigen compared to those without (105.4 ± 22pg/mg vs 10.2 ± 8.8 pg/mg) (P = 0.015). There wasno relationship between other antigens including CagA andproduction of IL-8. In conclusion, thelowmolecular-weight 33-35 K antigen may play animportant role in the pathogenesis of H. pylori-relateddisease.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1018850412148
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