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  • 1
    Electronic Resource
    Electronic Resource
    Apparatus for deformation tests of solids in liquid 3He (2000)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 71 (2000), S. 2833-2838 
    Details
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: An apparatus for deformation of solids in liquid 3He is constructed. Either tensile deformation or compression of a specimen can be performed by exchanging the assemblies in the 3He pot which has a capacity of about 30 cm3. The pulling rod for transmitting load from the tensile testing machine to the specimen runs inside the outlet tube of 3He, being isolated from 4He bath and almost free from mechanical friction. To measure the change in flow stress with the supernormal transition of superconducting metals, a superconducting magnet is mounted outside of the vacuum chamber which separates the 3He pot and the 4He bath. Under an applied load for plastic deformation the system is stably operative down to 0.6 K, while the lowest temperature achieved is 0.5 K. Some results on Ta and NaCl are presented. © 2000 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1150699
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  • 2
    Electronic Resource
    Electronic Resource
    034 
Application of Allogeneic Cultured Dermal Substitute to Skin Defects After Excision of Skin Cancer (2004)
    Oxford, UK; Malden, USA : Blackwell Science Inc
    Wound repair and regeneration 12 (2004), S. 0 
    Details
    ISSN: 1524-475X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Aim: This study was aimed to confirm the usefulness of allogeneic cultured dermal substitute (CDS) in the treatment of skin defects after excision of skin cancer. Methods: Eleven elderly patients (mean age of 76.3 year old) with skin cancer were included in this study. Most of the patients had basic disease such as diabetes or ASO. Allogeneic CDS used in the study were produced at Kitasato University. The CDS were applied to skin defects with exposing bone or tendon after oncological surgery, and changed once or twice a week until the open wound became suitable for autologous skin grafting or healed completely. Results: Healthy granulation tissue was formed to cover the exposed bone or tendon in all cases. Only in one case, the treatment with allogeneic CDS was abandoned owing to undesirable infection. In eight cases, appropriate wound beds acceptable for autologous skin graft were prepared. In other two cases, the skin defect became smaller and eventually closed without skin graft. In ten cases with or without skin graft, undesirable scar contracture was not observed over prolonged follow-up. Conclusions: Elderly patients with skin cancer provide reconstructive surgeons with challenging problems when bone or bare tendon is exposed after oncological surgery. Flap transfer might be complicated especially when the patients suffered from a basic disease such as diabetes or ASO. This study has confirmed that the use of allogeneic CDS is a safe and reliable method to achieve wound healing in those high-risk patients.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1067-1927.2004.abstractaw.x
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  • 3
    Electronic Resource
    Electronic Resource
    Clinical, histopathological and immunological distinction in two cases of IgA pemphigus (2002)
    Oxford, UK : Blackwell Science Ltd
    Clinical and experimental dermatology 27 (2002), S. 0 
    Details
    ISSN: 1365-2230
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Summary Two cases of IgA pemphigus, one of the subcorneal pustular dermatosis (SPD) type and one of the intraepidermal neutrophilic IgA dermatosis (IEN) type, are described. IgA anti-keratinocyte cell surface antibodies reacted only with the uppermost epidermis in the SPD type but with the entire epidermis in the IEN type. A cDNA transfection test for desmocollins (Dsc) revealed that IgA antibodies of the SPD type reacted with Dsc1, but the IEN type did not react with any of the Dsc isoforms (Dsc1–3). ELISA for desmoglein 1 (Dsg1) and Dsg3 showed that neither of the cases had antibodies to Dsg − of either the IgG or IgA subtype. These results confirm that the autoantigen in SPD-type IgA pemphigus is Dsc1, whereas the antigen in most cases of IEN-type IgA pemphigus is unknown.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2230.2002.01061.x
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  • 4
    Electronic Resource
    Electronic Resource
    Psoriasis bullosa acquisita (2002)
    Oxford, UK : Blackwell Science Ltd
    Clinical and experimental dermatology 27 (2002), S. 0 
    Details
    ISSN: 1365-2230
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Summary We report a 51-year-old man with a 20-year history of chronic plaque psoriasis who developed an autoimmune subepidermal blistering eruption that had clinical features of bullous pemphigoid, erythema multiforme and epidermolysis bullosa acquisita. Investigations revealed a 1 : 400 titre circulating and in vivo bound IgG autoantibody that mapped to the dermal side of 1 m NaCl-split skin and localized to the lower lamina lucida/upper lamina densa on immunogold electron microscopy. Immunoblotting, using dermal extracts, showed serum binding to antigens of ≈ 200- and ≈ 260 kDa. Indirect immunofluorescence microscopy, using the patient's serum on archival skin sections taken from selected individuals with different forms of inherited epidermolysis bullosa as substrate, showed normal basement membrane labelling on all samples apart from recessive dystrophic epidermolysis bullosa skin (with inherent mutations in the type VII collagen gene): in these cases there was a complete absence of immunostaining. Clinically, the patient responded rapidly to combination treatment with intravenous immunoglobulin and oral corticosteroids, dapsone and mycophenolate mofetil. Autoimmune subepidermal blistering has been reported in other patients with psoriasis, although no specific target antigen has ever been determined. Our study provides preliminary evidence that, for this patient at least, the autoantibody may be targeted against a skin component closely associated with type VII collagen (the epidermolysis bullosa acquisita antigen). Therefore, we propose the term ‘psoriasis bullosa acquisita’ for this and possibly other patients with similar skin eruptions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2230.2002.01100.x
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  • 5
    Electronic Resource
    Electronic Resource
    Bullous pemphigoid associated with dermatomyositis successfully controlled with minocycline (2003)
    Oxford, UK : Blackwell Science Ltd
    Clinical and experimental dermatology 28 (2003), S. 0 
    Details
    ISSN: 1365-2230
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2230.2003.01355.x
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  • 6
    Electronic Resource
    Electronic Resource
    Immunolocalization of target autoantigens in IgA pemphigus (2004)
    Oxford, UK : Blackwell Science Ltd
    Clinical and experimental dermatology 29 (2004), S. 0 
    Details
    ISSN: 1365-2230
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: IgA pemphigus is a rare autoimmune bullous disease characterized by IgA deposition at keratinocyte cell surfaces. Clinically and histologically, IgA pemphigus is divided into two major subtypes: subcorneal pustular dermatosis (SPD) type and intraepidermal neutrophilic IgA dermatosis (IEN) type. Using cDNA transfection and living cell immunofluorescence, we previously showed that desmocollin 1, one of the desmosomal cadherins, is the autoantigen in SPD-type IgA pemphigus, but the autoantigen in IEN type is still unclear. In the present study we investigated antigen localization by postembedding immunoelectron microscopy. We examined three sera each of SPD-type and IEN-type IgA pemphigus. In SPD-type, gold particles were observed predominantly in the extracellular spaces between keratinocytes at desmosomes, although a few particles were observed in the intracellular domain at the desmosomal attachment plaques. In IEN type, the gold particles were observed mainly in the intercellular spaces in nondesmosomal areas. These results provide evidence that the IgA in the sera of SPD-type IgA pemphigus reacts with the extracellular domain of desmocollins. In contrast, the autoantigen for IEN type may in fact not be a component of desmosomes. IEN-type IgA pemphigus may be the first member of the pemphigus group of autoimmune bullous dermatoses that reacts with a nondesmosomal transmembranous protein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2230.2004.01436.x
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  • 7
    Electronic Resource
    Electronic Resource
    Cicatricial pemphigoid: IgA and IgG autoantibodies target epitopes on both intra- and extracellular domains of bullous pemphigoid antigen 180 (2001)
    Oxford, UK : Blackwell Science Ltd
    British journal of dermatology 145 (2001), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background Cicatricial pemphigoid (CP) is an autoimmune subepidermal blistering disease where autoantibodies target various components of the dermal–epidermal junction, including the bullous pemphigoid antigen 180 (BP180). Objective We determined the exact specificity of circulating IgG and IgA autoantibodies to BP180 in a large number of CP patients. Methods Twenty-six consecutive CP sera were analysed by Western blotting using a panel of cell-derived and recombinant proteins covering the entire BP180 molecule. Results Circulating autoantibodies were detected in all CP sera. Seven sera reacting with laminin-5 were excluded from further analyses; the remaining 19 sera recognized BP180, including six sera (32%) that showed only IgA reactivity to this protein. With the combined use of the soluble BP180 ectodomain (LAD-1) and recombinant BP180 NC16A, 16 of these 19 CP sera (84%) targeted BP180. IgG reactivity was preferentially found against NC16A, whereas IgA antibodies predominantly recognized LAD-1. Thirty-two per cent of the BP180-reative sera revealed reactivity with the intracellular domain of this protein. Conclusions Our findings demonstrate that autoantibodies in CP target epitopes on both extra- and intracellular domains of BP180 and highlight the importance of testing for both IgG and IgA reactivity in these patients' sera.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2133.2001.04471.x
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  • 8
    Electronic Resource
    Electronic Resource
    Autoantibodies in anti-p200 pemphigoid stain skin lacking laminin 5 and type VII collagen (2000)
    Oxford, UK : Blackwell Science Ltd
    British journal of dermatology 143 (2000), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: We report the case of a patient with a widespread bullous skin disease and linear deposits of IgG and C3 at the dermal–epidermal junction using direct immunofluorescence microscopy. Indirect immunofluorescence analysis demonstrated circulating IgG autoantibodies that stained, like autoantibodies to laminin 5 and type VII collagen, the dermal side of 1 mol L−1 NaCl-split human skin. By immunoblotting dermal extracts, the patient’s serum, like serum samples from two control patients, reacted with a 200-kDa protein. Using immunoelectron microscopy, the serum labelled a component of the lower lamina lucida, but not the lamina densa/sublamina densa region, distinguishing this from the type VII collagen localization pattern. By immunofluorescence microscopy on skin sections from patients lacking either laminin 5 (Herlitz’s epidermolysis bullosa) or type VII collagen (recessive dystrophic epidermolysis bullosa of Hallopeau–Siemens), the patient’s serum retained reactivity with these test substrates. The patient’s disease responded rapidly to the use of topical corticosteroids and lesions healed without scarring or milia formation. Our results provide strong evidence for the hypothesis that the 200 kDa autoantigen is different from laminin 5 and type VII collagen. For this new disease, we propose the designation ‘anti-p200 pemphigoid’.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2133.2000.03841.x
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  • 9
    Electronic Resource
    Electronic Resource
    The clinical transition between pemphigus foliaceus and pemphigus vulgaris correlates well with the changes in autoantibody profile assessed by an enzyme-linked immunosorbent assay (2001)
    Oxford, UK : Blackwell Science Ltd
    British journal of dermatology 144 (2001), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background There are a number of reports of pemphigus with clinical shifting between pemphigus foliaceus (PF) and pemphigus vulgaris (PV). On the other hand, a novel enzyme-linked immunosorbent assay (ELISA) against recombinant baculoproteins of desmoglein 1 (Dsg1) (PF antigen) and Dsg3 (PV antigen) has been established and found to be extremely sensitive and specific. Objectives To characterize the change in the antibody profiles in a series of pemphigus cases with mixed features of PF and PV by various methods, including the novel ELISA. Patients/methods Sera were obtained from eight cases undergoing a shift between PF and PV and three cases of coexistent PF and PV. The autoantigens were analysed by ELISA, as well as by immunofluorescence using normal human skin sections and immunoblotting using normal human epidermal extracts. Results The results of the ELISA, immunofluorescence and immunoblotting studies showed that the transition between PF and PV correlates well with the changes of autoantibodies against either Dsg1 or Dsg3. Conclusions The clinical phenotype at each stage is defined by the anti-Dsg antibody profile in the serum of these pemphigus patients showing mixed features of PF and PV. In addition, ELISA using recombinant baculoproteins was particularly useful in distinguishing PF and PV.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2133.2001.04227.x
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  • 10
    Electronic Resource
    Electronic Resource
    Pemphigoid nodularis with IgA autoantibodies against the intracellular domain of desmoglein 1 (2000)
    Oxford, UK : Blackwell Science Ltd
    British journal of dermatology 142 (2000), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Pemphigoid nodularis is a rare variant of bullous pemphigoid. We report a 49-year-old Japanese male with clinical and histopathological features of pemphigoid nodularis including circulating and in vivo-bound IgG antibasement membrane zone antibodies and IgA anti-intercellular antibodies. Although the precise molecular target of the IgG autoantibodies could not be determined, intriguingly, immunoblotting showed that the IgA in the patient’s serum reacted with the intracellular domain of desmoglein 1, the target antigen in cases of pemphigus foliaceus. However, the IgA did not react with the extracellular domain of desmoglein 1 in sensitive enzyme-linked immunosorbent assay studies using a baculovirus system. These results suggest therefore that these IgA antibodies may possibly not be pathogenic. The mechanism for the production of different autoantibodies is unknown, but this case provides further illustration of the atypical skin immunoreactants often seen in this unusual subtype of bullous pemphigoid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2133.2000.03257.x
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