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  • Springer  (27)
  • Blackwell Futura Publishing, Inc.  (1)
  • Munksgaard International Publishers  (1)
  • 2000-2004  (16)
  • 1960-1964  (13)
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  • 1
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Rapid changes of enzyme activity are obtained by post-translational modification of cysteine residues of some chloroplast enzymes. Individual fine-tuning is achieved by specific factors acting upon the redox cycle. In order to study the regulatory properties of these enzymes, they are purified from leaves or in a recombinant form from Escherichia coli. The various factors acting upon the enzyme in vivo can be simulated in vitro. However, in these studies, some subtle technical problems can be encountered. Two cases are presented in this article, and an attempt is made to explain some previous, seemingly contradictory results. The Calvin-cycle enzyme glyceraldehyde 3-phosphate dehydrogenase in its less active A8B8 form can be dissociated and thereby activated in vitro simply by diluting out the protein. On the other hand, activation requires the presence of reduced thioredoxin (Td) and an increase in ionic strength when performed at a high protein concentration, as present in vivo. Chloroplast fructose-1,6-bisphosphatase (FBPase) is purified from E. coli as an enzyme similar to that purified from leaves. However, using the standard protocol for lysis of the bacteria leads to a form with some unusual properties as changed isoelectric point, lack of Ca2+/fructose-1,6-bisphosphate (FBP) dependency of reductive activation, and lack of activity at high pH and high Mg2+ concentration. These observations are used in order to better understand the characteristics of the activation/inactivation process.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1540-8159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: BREUR, J.M.P.J., et al.: Pacemaker Therapy in Isolated Congenital Complete Atrioventricular Block. The aim of this study was to evaluate the effect of pacemaker (PM) therapy in patients with isolated congenital complete atrioventricular block (CCAVB). Patients with CCAVB eventually qualify for PM implantation, however, timing remains controversial. Retrospective evaluation of left ventricular end-diastolic diameter (LVEDD), shortening fraction (SF), and cardiothoracic ratio (CTR) in 149 CCAVB patients, before, at, and after PM implantation was carried out. LVEDD shows an average increase of 0.48%/month in non-PM patients, and an average decrease of 0.88%/month in PM patients. SF shows an average increase of 0.10%/month in non-PM, and an average decrease of 0.32%/month in PM patients. CTR shows an average increase of 0.02%/month in non-PM, and an average decrease of 0.19%/month in PM patients. The difference between the non-PM and PM groups is significant (P = 0.05) for all variables. Symptomatic patients show no significant change in LVEDD after PM therapy (from 66.5% before to 68.5% after PM therapy). Asymptomatic patients do show a significant (P 〈 0.001) decrease in LVEDD after PM therapy (from 78.4% before to 73.3% after PM therapy). CTR does not differ significantly between symptomatic and asymptomatic patients before PM therapy (58% and 57%, respectively). CTR does differ significantly (P 〈 0.001) between symptomatic and asymptomatic patients after PM therapy (52% and 48%, respectively). Heart size and SF are increased in most patients with isolated CCAVB. PM implantation is associated with a decrease in heart size and normalization of SF in most patients. Indications for PM therapy in children may require reevaluation in asymptomatic patients with increased cardiac size and decreased cardiac function.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    European food research and technology 211 (2000), S. 346-348 
    ISSN: 1438-2385
    Keywords: Key wordsCraterellus ; Gomphidius ; Higher fungi ; Hydnum ; Hygrophorus, Laccaria ; Trypsin inhibitors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Laccaria and Hygrophorus species, Craterellus cornucopioides, Gomphidius glutinosus, Macrolepiota rhacodes and Hydnum repandum. The inhibiting activities found were higher than those of cereals but lower than those of some fabaceous plants.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Naturwissenschaften 51 (1964), S. 240-240 
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Naturwissenschaften 48 (1961), S. 156-157 
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-0584
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary The isolation of a plant protease (bromelin) from the juice of ananas is described. The preparation was specific and qualified for the demonstration of blood factors and antibodies of the ABO-, Rh-, K-, Fy-, MN- and P-system. Lea respectively its antibody cannot always be demonstrated surely.
    Notes: Zusammenfassung Es wird die Herstellung einer Pflanzenprotease (Bromelin) aus dem Saft der Ananasfrucht beschrieben. Das gewonnene Präparat erwies sich als spezifisch und ist beim Nachweis der Blutfaktoren und Antikörper des ABO-, Rh-, K-, Fy-, MN- und P-Systems geeignet. Die Eigenschaft Lea bzw. ihr Antikörper ist nicht immer sicher nachweisbar.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Archives of dermatological research 213 (1961), S. 421-425 
    ISSN: 1432-069X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Virchows Archiv 335 (1962), S. 428-451 
    ISSN: 1432-2307
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Eingehende makroskopische und histologische Beschreibung von 4 Fällen von sekundärer Sarkomentwicklung auf dem Boden einer einkammerigen Knochencyste. Im Sarkom wird das Strukturprinzip der Knochencyste wiederholt. Die gleichen Veränderungen finden sich in einem 5. Fall von Knochensarkom, welches sich auf dem Boden eines cystisch umgewandelten Knochenmarkinfarktes entwickelt hatte.
    Notes: Summary Four typical, large, hollow simple bone cysts with a sarcoma arising in the cyst wall are described. The range of patterns in the sarcomas duplicated the range of patterns that may be found in the walls of bone cysts. The same variation was present in another sarcoma arising from the wall of a cystic cavity that was produced by bone marrow infarction.
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  • 9
    ISSN: 1432-0568
    Keywords: Key words Blood vessel walls ; Intima thickening ; Lamina elastica interna splitting ; Morphology ; Elastin quantification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Although pre-eclampsia (PE) is often associated with fetal hypoxia, hypertension and/or disturbed function of the fetal circulation, the effect of these altered hemodynamic parameters on the structure and composition of umbilical vessels has not been systematically investigated before. Therefore, this study focuses on PE-associated changes of the elastic fibre system in umbilical cord vessels investigated by light and electron microscopy, immunocytochemistry and biochemistry. In umbilical cord veins, no changes in thickness of the vessel wall or of any sublayer were observed. However, the internal elastic lamina of the veins was split in 80% of the PE-group in contrast to 20% in uncomplicated pregnancies. This effect was significant (α 〈0.01) from 36 weeks of gestation onwards. In umbilical cord arteries, the entire arterial vessel wall was found to be 15% thicker in PE than in uncomplicated pregnancies. The enlargement was caused by an increase of both the tunica intima and tunica media. The thickening of the tunica intima was attributed to a migration of smooth muscle cells towards the endothelium, accompanied by a splitting of the internal elastic lamina. Compared to uncomplicated pregnancies, smooth muscle cells of arteries and veins in PE showed a metabolic activation demonstrated by highly dilated endoplasmic reticulum. A semiquantitative score method as well as a quantitative dot blot assay indicated a PE-associated reduction of elastin expression in the arterial vessel walls. In summary, PE obviously induces a decrease of the elastin content accompanied by a thickening of the vessel wall in umbilical cord arteries. This remodeling of the elastic fibre system, together with an increased migration of smooth muscle cells, might represent part of the functional adaptation system of the umbilical cord arteries on the altered hemodynamic conditions in PE.
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  • 10
    ISSN: 1432-1084
    Keywords: Key words: Renal arteries – Stenosis or obstruction – Renal arteries – Transluminal angioplasty – Stents and prostheses – Renal hypertension
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. The aim of this study was to determine long-term success of flexible tantalum stents for the treatment of ostial and truncal renal artery stenosis. Since 1989, flexible tantalum stents (type Strecker) were implanted in 34 patients (36 arteries, 25 ostial lesions, 11 truncal lesions) with uncontrollable renovascular hypertension, 9 of them in association with renal insufficiency. Stents were placed unilaterally in 32 patients, and bilaterally in 2 patients for the treatment of renal artery stenosis. Thirty-five of 36 lesions were atherosclerotic, including 5 recurrencies after previous percutaneous transluminal renal angioplasty (PTRA). One patient had Takayasu arteritis. Stents were implanted after unsuccessful PTRA of 11 truncal and 23 ostial lesions, and as a primary procedure in 2 ostial lesions. Follow-up examinations included blood pressure measurement, determination of serum creatinine level, color duplex sonography, or angiography. The technical success rate was 92 %. Technical failure included incorrect stent placement (1 of 36 lesions, 2.8 %), and stent dislocation (2 of 36 lesions, 5.6 %), and two stents were retrieved percutaneously. In one case of Takayasu arteritis, residual stenosis of 40 % was observed. After technically successful stent placement, 77 % of patients became normotensive with or without medication. In the remaining patients there was partial improvement with blood pressure between 140 and 180 mmHg. Renal function improved in 76 % of patients (completely in 3 of 8, 38 %; and partially in 3 of 8, 38 %). Primary patency rate including all stented lesions and initial technical failures was 82.4 % ± 6.8 (1 year) and 82.4 % ± 9.2 (3 years). After technically successful stent placement, patency rates were 89.9 ± 5.6 % (1 year), and 89.9 ± 7.6 % (3 years). For ostial lesions, primary patency rate was 87.9 ± 6.7 % (1 year) and 87.9 ± 9.2 % (3 years). Placement of flexible tantalum stents in renal arteries is technically demanding, especially in ostial lesions. Once placed successfully, stent patency rate is excellent.
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