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  • OceanRep  (3)
  • Elsevier  (3)
  • 2005-2009  (3)
  • 1
    Publication Date: 2019-09-23
    Description: Experimental setups to study modes of inorganic carbon acquisition and fixation rates by marine phytoplankton commonly make use of so-called disequilibrium techniques. The chemical or isotopic disequilibrium, either caused by phytoplankton cells taking up inorganic carbon or by a small disturbance of the isotopic equilibrium in the carbonate system, requires to account for the relatively slow chemical interconversion of carbon dioxide (CO2) to bicarbonate (HCO3−) in seawater. Because in such experiments a constant pH is a prerequisite, pH buffers are generally used. However, a possible influence of such buffers on the kinetics of the carbonate system has hitherto not been investigated. Here, a model of the carbonate system in seawater is employed to show how pH buffers are operating. Furthermore, a new approach is presented to determine the rate constants, k+ and k−, for the conversion reaction of CO2 to HCO3− and vice versa, by means of membrane inlet mass spectrometry (MIMS). For the two pH buffers tested (HEPES and BICINE) it is shown that measured rate constants are in good agreement with calculated values for k+ and k− in a pH range of 7 to 8.5 and at temperatures from 10 to 25 °C.
    Type: Article , PeerReviewed
    Format: text
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  • 2
    Publication Date: 2017-01-30
    Description: Throughout the last similar to 900 kyr, the Late Pleistocene, Earth has experienced periods of cold glacial climate, punctuated by seven abrupt transitions to warm interglacials, the so-called terminations. Although most of glacial ice is located in the Northern Hemisphere (NH), the Southern Hemisphere (SH) seems to play a crucial role in deglaciation. Variation in the seasonal distribution of solar insolation is one candidate for the cause of these climatic shifts. But so far, no simple mechanism has been identified. Here we present a mathematical analysis of variations in midsummer insolation in both hemispheres at 65 degrees latitude. Applying this analysis to the entire Pleistocene, the last 2 Myr, we find that prior to each termination the insolation in both hemispheres increases in concert, with a SH lead. Introducing time and energy thresholds to these overlaps, calculated times for the onsets of the seven terminations by this insolation canon (exceptional overlaps meeting the two threshold prerequisites) are similar to 23, 139, 253, 345, 419, 546 and 632 kyr BP, perfectly matching the geologic record. The timing originates from the interplay between the two orbital parameters obliquity and precession, explaining why terminations occur at integer multiple of the precessional cycle. There is no such constellation between I and 2 Myr BP, the Early Pleistocene, in agreement with Earth's climate at that time. This change in orbital forcing coincides with the Mid Pleistocene Revolution, separating the Late from the Early Pleistocene. Therefore, we hypothesize that the insolation canon is the trigger for glacial terminations. (c) 2006 Elsevier B.V. All rights reserved.
    Type: Article , PeerReviewed
    Format: text
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  • 3
    Publication Date: 2019-09-23
    Description: Iron is limiting phytoplankton productivity in large parts of today's oceans, the so-called HNLC (high nutrient low chlorophyll) areas. It is a key component in photosynthesis during which inorganic carbon fixation in most phytoplankton species is sustained by so-called carbon concentrating mechanisms (CCMs). Here we investigate CCM regulation in the coccolithophore Emiliania huxleyi in response to varying degrees of iron limitation by means of membrane-inlet mass spectrometry. Compared to iron replete conditions rates of both active CO2 and HCO-3 uptake were markedly reduced under iron limitation leading to significantly diminished growth rates. Moreover, there was a concomitant decrease in CCM efficiency, reflected in an increased CO2 loss from the cell in relation to carbon fixation. Under such conditions higher values for carbon isotope fractionation (∈P) would be expected. However, direct measurements of ∈P showed that carbon isotope fractionation was insensitive to changes in growth rates and CCM activity. This can be explained by concomitant changes in internal DIC fluxes in and out of the chloroplast as demonstrated with a simple cell model comprising two compartments. Thus, carbon isotope fractionation reflects the ability of phytoplankton to actively control their inorganic carbon acquisition depending on environmental conditions. The insensitivity of carbon isotope fractionation to changes in the availability of iron could be of interest for paleoreconstructions in the HNLC areas of today's oceans.
    Type: Article , PeerReviewed
    Format: text
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