Publication Date:
2022-05-25
Description:
Author Posting. © The Authors, 2010. This is the author's version of the work. It is posted here by permission of Oxford University Press for personal use, not for redistribution. The definitive version was published in Molecular Biology and Evolution 27 (2010): 2211-2215, doi:10.1093/molbev/msq129.
Description:
Conserved interactions among proteins or other molecules can provide strong evidence
for coevolution across their evolutionary history. Diverse phylogenetic methods have
been applied to identify potential coevolutionary relationships. In most cases, these
methods minimally require comparisons of orthologous sequences and appropriate
controls to separate effects of selection from the overall evolutionary relationships. In
vertebrates, androgen receptor (AR) and cytochrome p450 aromatase (CYP19) share an
affinity for androgenic steroids, which serve as receptor ligands and enzyme substrates.
In a recent study, Tiwary and Li (2009) reported that AR and CYP19 displayed a
signature of ancient and conserved interactions throughout all of the Eumetazoa (i.e.,
cnidarians, protostomes, and deuterostomes). Because these findings conflicted with a
number of previous studies, we reanalyzed the data set used by Tiwary and Li. First, our
analyses demonstrate that the invertebrate genes used in the previous analysis are not
orthologous sequences, but instead represent a diverse set of nuclear receptors and
cytochrome p450 enzymes with no confirmed or hypothesized relationships with
androgens. Second, we show that (1) their analytical approach, which measures
correlations in evolutionary distances between proteins, potentially led to spurious
significant relationships due simply to conserved domains and (2) control comparisons
provide positive evidence for a strong influence of evolutionary history. We discuss how
corrections to this method and analysis of key taxa (e.g., duplications in the teleost fish
and suiform lineages) can inform investigations of the coevolutionary relationships
between androgen receptor and aromatase.
Description:
AMR was supported by the Postdoctoral Scholar Program at the Woods Hole
Oceanographic Institution, with funding provided by The Beacon Institute for Rivers and
Estuaries, and AMT was supported by WHOI Assistant Scientist Endowed Support.
Keywords:
Androgen receptor
;
Aromatase
;
Correlated evolution
;
Cytochrome p450
;
Nuclear receptor
Repository Name:
Woods Hole Open Access Server
Type:
Preprint
Format:
application/pdf
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