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  • Cytochrome p450  (1)
  • Developmental biology  (1)
  • 2010-2014  (2)
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  • 2010-2014  (2)
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  • 1
    Publication Date: 2022-05-25
    Description: Author Posting. © The Authors, 2010. This is the author's version of the work. It is posted here by permission of Oxford University Press for personal use, not for redistribution. The definitive version was published in Molecular Biology and Evolution 27 (2010): 2211-2215, doi:10.1093/molbev/msq129.
    Description: Conserved interactions among proteins or other molecules can provide strong evidence for coevolution across their evolutionary history. Diverse phylogenetic methods have been applied to identify potential coevolutionary relationships. In most cases, these methods minimally require comparisons of orthologous sequences and appropriate controls to separate effects of selection from the overall evolutionary relationships. In vertebrates, androgen receptor (AR) and cytochrome p450 aromatase (CYP19) share an affinity for androgenic steroids, which serve as receptor ligands and enzyme substrates. In a recent study, Tiwary and Li (2009) reported that AR and CYP19 displayed a signature of ancient and conserved interactions throughout all of the Eumetazoa (i.e., cnidarians, protostomes, and deuterostomes). Because these findings conflicted with a number of previous studies, we reanalyzed the data set used by Tiwary and Li. First, our analyses demonstrate that the invertebrate genes used in the previous analysis are not orthologous sequences, but instead represent a diverse set of nuclear receptors and cytochrome p450 enzymes with no confirmed or hypothesized relationships with androgens. Second, we show that (1) their analytical approach, which measures correlations in evolutionary distances between proteins, potentially led to spurious significant relationships due simply to conserved domains and (2) control comparisons provide positive evidence for a strong influence of evolutionary history. We discuss how corrections to this method and analysis of key taxa (e.g., duplications in the teleost fish and suiform lineages) can inform investigations of the coevolutionary relationships between androgen receptor and aromatase.
    Description: AMR was supported by the Postdoctoral Scholar Program at the Woods Hole Oceanographic Institution, with funding provided by The Beacon Institute for Rivers and Estuaries, and AMT was supported by WHOI Assistant Scientist Endowed Support.
    Keywords: Androgen receptor ; Aromatase ; Correlated evolution ; Cytochrome p450 ; Nuclear receptor
    Repository Name: Woods Hole Open Access Server
    Type: Preprint
    Format: application/pdf
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  • 2
    Publication Date: 2022-05-26
    Description: Author Posting. © The Author(s), 2014. This is the author's version of the work. It is posted here by permission of Elsevier for personal use, not for redistribution. The definitive version was published in Zoology 118 (2015): 135-140, doi:10.1016/j.zool.2014.06.005.
    Description: The third Nematostella vectensis Research Conference took place in December 2013 in Eilat, Israel, as a satellite to the 8th International Conference on Coelenterate Biology. The starlet sea anemone, Nematostella vectensis, has emerged as a powerful cnidarian model, in large part due to the extensive genomic and transcriptomic resources and molecular approaches that are becoming available for Nematostella, which were the focus of several presentations. In addition, research was presented highlighting the broader utility of this species for studies of development, circadian rhythms, signal transduction, and gene–environment interactions.
    Description: Research in the authors’ laboratories on Nematostella is supported by National Science Foundation grants MCB-1057354 to A.M.T. and MCB-0924749 to T.D.G. Travel support for the meeting was provided to T.D.G. by Illumina, Inc. (San Diego, CA, USA), to A.M.R. by the University of North Carolina at Charlotte, and to A.M.T. by the Israel–US Binational Science Foundation (Jerusalem, Israel).
    Keywords: Nematostella vectensis ; Cnidaria ; Genomics ; Transcriptomics ; Developmental biology
    Repository Name: Woods Hole Open Access Server
    Type: Preprint
    Format: application/pdf
    Location Call Number Limitation Availability
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