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  • PANGAEA  (3)
  • Frontiers
  • Inter Research
  • 2010-2014  (3)
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  • 1
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    PANGAEA
    In:  Supplement to: Piontek, Judith; Lunau, Mirko; Händel, Nicole; Borchard, Corinna; Wurst, Mascha; Engel, Anja (2010): Acidification increases microbial polysaccharide degradation in the ocean. Biogeosciences, 7(5), 1615-1625, https://doi.org/10.5194/bg-7-1615-2010
    Publication Date: 2023-11-15
    Description: With the accumulation of anthropogenic carbon dioxide (CO2), a proceeding decline in seawater pH has been induced that is referred to as ocean acidification. The ocean's capacity for CO2 storage is strongly affected by biological processes, whose feedback potential is difficult to evaluate. The main source of CO2 in the ocean is the decomposition and subsequent respiration of organic molecules by heterotrophic bacteria. However, very little is known about potential effects of ocean acidification on bacterial degradation activity. This study reveals that the degradation of polysaccharides, a major component of marine organic matter, by bacterial extracellular enzymes was significantly accelerated during experimental simulation of ocean acidification. Results were obtained from pH perturbation experiments, where rates of extracellular alpha- and beta-glucosidase were measured and the loss of neutral and acidic sugars from phytoplankton-derived polysaccharides was determined. Our study suggests that a faster bacterial turnover of polysaccharides at lowered ocean pH has the potential to reduce carbon export and to enhance the respiratory CO2 production in the future ocean.
    Keywords: alpha-glucosidase activity per cell; Bacteria; Bacteria, abundance, standard deviation; beta-glucosidase activity per cell; Carbon, organic, particulate; Carbon, organic, particulate, standard deviation; Cell-specific glucosidase activity; Cell-specific glucosidase activity, standard deviation; Combined glucose loss; Combined glucose loss, standard deviation; Element analyser CNS, EURO EA; EPOCA; European Project on Ocean Acidification; Experimental treatment; FACSCalibur flow-cytometer (Becton Dickinson); High Performance anion-exchange chromatography; Light:Dark cycle; Measured; Particulate organic carbon loss; Particulate organic carbon loss, standard deviation; pH; Polysacchrides loss; Polysacchrides loss, standard deviation; Radiation, photosynthetically active; Sample ID; see reference(s); Temperature, water; Time, incubation; WTW 340i pH-analyzer and WTW SenTix 81-electrode
    Type: Dataset
    Format: text/tab-separated-values, 452 data points
    Location Call Number Limitation Availability
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  • 2
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    Unknown
    PANGAEA
    In:  GEOMAR - Helmholtz Centre for Ocean Research Kiel | Supplement to: Engel, Anja; Piontek, Judith; Grossart, Hans-Peter; Riebesell, Ulf; Schulz, Kai Georg; Sperling, Martin (2014): Impact of CO2 enrichment on organic matter dynamics during nutrient induced coastal phytoplankton blooms. Journal of Plankton Research, 36(3), 641-657, https://doi.org/10.1093/plankt/fbt125
    Publication Date: 2024-02-01
    Description: A mesocosm experiment was conducted to investigate the impact of rising fCO2 on the build-up and decline of organic matter during coastal phytoplankton blooms. Five mesocosms (~38 m³ each) were deployed in the Baltic Sea during spring (2009) and enriched with CO2 to yield a gradient of 355-862 µatm. Mesocosms were nutrient fertilized initially to induce phytoplankton bloom development. Changes in particulate and dissolved organic matter concentrations, including dissolved high-molecular weight (〉1 kDa) combined carbohydrates, dissolved free and combined amino acids as well as transparent exopolymer particles (TEP), were monitored over 21 days together with bacterial abundance, and hydrolytic extracellular enzyme activities. Overall, organic matter followed well-known bloom dynamics in all CO2 treatments alike. At high fCO2, higher dPOC:dPON during bloom rise, and higher TEP concentrations during bloom peak, suggested preferential accumulation of carbon-rich components. TEP concentration at bloom peak was significantly related to subsequent sedimentation of particulate organic matter. Bacterial abundance increased during the bloom and was highest at high fCO2. We conclude that increasing fCO2 supports production and exudation of carbon-rich components, enhancing particle aggregation and settling, but also providing substrate and attachment sites for bacteria. More labile organic carbon and higher bacterial abundance can increase rates of oxygen consumption and may intensify the already high risk of oxygen depletion in coastal seas in the future.
    Keywords: BIOACID; Biological Impacts of Ocean Acidification; SOPRAN; Surface Ocean Processes in the Anthropocene
    Type: Dataset
    Format: application/zip, 2 datasets
    Location Call Number Limitation Availability
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  • 3
    Publication Date: 2024-03-15
    Keywords: 19-Butanoyloxyfucoxanthin; 1-Iodoethane; 1-Iodopropane; 2-Iodopropane; Algae, biomass as carbon; Algae, fatty acids; Algae abundance; Alkaline phosphatase; Alkalinity, Gran titration (Gran, 1950); Alkalinity, total; Alloxanthin; alpha-Carotene, beta,epsilon-Carotene; Ammonium; Aphanizophyll; Aragonite saturation state; Arctic; Bacteria; Bacteria, biomass as carbon; Bacteria, fatty acids; Bacteria, high DNA fluorescence; Bacteria, low DNA fluorescence; Bacterial/community respiration, oxygen, ratio; Bacterial biomass production of carbon; Bacterial biomass production of carbon, standard deviation; Bacterial production; Bacterial production, standard deviation; beta-Carotene, beta,beta-Carotene; Bicarbonate ion; BIOACID; Biogenic silica; Biological Impacts of Ocean Acidification; Biomass/Abundance/Elemental composition; Bromochloromethane; Bromoiodomethane; Calanus finmarchicus, δ13C; Calcite saturation state; Calculated; Calculated from linear regression; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, particulate; Carbon, organic, dissolved; Carbon, organic, particulate; Carbon, total, particulate; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, flux per mesocosm; Chloroiodomethane; Chlorophyll a; Chlorophyll a, areal concentration; Chlorophyll b; Chlorophyll c1+c2; Chlorophyll c3; Chlorophytes; Cirripedia, larvae, δ13C; Coast and continental shelf; Community composition and diversity; Coulometry; Cryptophytes; Cyanobacteria, biomass per area; DATE/TIME; delta 13C labeling method; Diadinoxanthin; Diatoxanthin; Dibromochloromethane; Dibromomethane; Diiodomethane; Dimethyl sulfide, dissolved; Dimethylsulfoniopropionate; Entire community; EPOCA; EUR-OCEANS; European network of excellence for Ocean Ecosystems Analysis; European Project on Ocean Acidification; Exudation as determined by 14C DOC production; Exudation as determined by 14C DOC production, standard deviation; Field experiment; Flow cytometry; Fucoxanthin; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gas chromatography - Mass spectrometry (GC-MS); GC-PFPD; Gross community production of oxygen; Hand-operated CTD (Sea&Sun Technology, CTD 60M); High Performance Liquid Chromatography (HPLC); Identification; Iodomethane; Kongsfjorden-mesocosm; MESO; Mesocosm experiment; Mesocosm or benthocosm; Myxoxanthophyll; Nanoplankton; Neoxanthin; Net community production, standard deviation; Net community production of carbon dioxide; Net community production of oxygen; Nitrate; Nitrite; Nitrogen, organic, dissolved; Nitrogen, organic, particulate; Nitrous oxide; OA-ICC; Ocean Acidification International Coordination Centre; Other metabolic rates; Oxygen; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; Peridinin; pH; Phosphate; Phosphorus, organic, dissolved; Phosphorus, organic, particulate; Phytoplankton, biomass per area; Picophytoplankton; Polar; Prasinoxanthin; Primary production/Photosynthesis; Primary production of POC as determined by 14C POC production; Primary production of POC as determined by 14C POC production, standard deviation; Pulsed flame photometric detector - gas chromatography; Respiration; Respiration, oxygen, bacterial; Respiration, oxygen, bacterial, standard error; Respiration, oxygen, community; Respiration, oxygen, community, standard error; Salinity; Sample comment; Sigmas; Silicon; Svalbard; Temperature, water; Thymidine incorporation; Time, incubation; Transfer velocity, carbon dioxide; Transfer velocity, dimethyl sulfide; Transfer velocity, nitrous oxide; Tribromomethane; Turbidity (Formazin Turbidity Unit); Violaxanthin; Viral abundance; Virus/bacteria ratio; Viruses; Water content of mesocosm; Zeaxanthin; Δδ13C; δ13C, algae; δ13C, bacteria; δ13C, dissolved inorganic carbon; δ13C, dissolved organic carbon; δ13C, particulate organic carbon
    Type: Dataset
    Format: text/tab-separated-values, 35076 data points
    Location Call Number Limitation Availability
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