Keywords:
Lipids -- Analysis.
;
Electronic books.
Description / Table of Contents:
This well-known and highly successful book was first published in 1973 and has been completely re-written in subsequent editions (published in 1982 and 2003). This new Fourth Edition has become necessary because of the pace of developments in mass spectrometry of intact lipids, which has given recognition of lipid analysis and 'lipidomics' as a distinct science. To bring the book up to date with these developments, author William W. Christie is joined by co-author Xianlin Han. Although devoting considerable space to mass spectrometry and lipidomics, Lipid analysis remains a practical guide, in one volume, to the complexities of the analysis of lipids. As in past editions, it is designed to act as a primary source, of value at the laboratory bench rather than residing on a library shelf.Lipid analysis deals with the isolation, separation, identification and structural analysis of glycerolipids, including triacylglycerols, phospholipids, sphingolipids, and the various hydrolysis products of these. The chapters follow a logical sequence from the extraction of lipids to the isolation and characterization of particular lipid classes and of molecular species of each, and to the mass spectrometric analysis of lipids and lipidomics.The new influence of mass spectrometry is due mainly to the development of electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI). Most emphasis in this book is placed on ESI, which is enabling structural characterization of different lipid classes and the identification of novel lipids and their molecular species.
Type of Medium:
Online Resource
Pages:
1 online resource (447 pages)
Edition:
4th ed.
ISBN:
9780857097866
Series Statement:
Oily Press Lipid Library Series ; v.24
URL:
https://ebookcentral.proquest.com/lib/geomar/detail.action?docID=1829195
DDC:
547.77046
Language:
English
Note:
Cover -- Lipid analysis: ISOLATION, SEPARATION, IDENTIFICATION AND LIPIDOMIC ANALYSIS -- Copyright -- Preface -- Contents -- List of Abbreviations -- PART 1 Introducing lipids and their analysis -- CHAPTER 1 Lipids: their structures and occurrence -- A. Introduction -- B. Definitions -- C. Fatty acids -- D. The principal simple lipids and complex glycerolipids of animal and plant tissues -- E. Sphingolipids -- CHAPTER 2 Chromatographic analysis of lipids: general principles -- A. A statement of the problem -- B. The theory of chromatography -- C. Modes of separation in chromatography -- D. Gas chromatography -- E. High-performance liquid chromatography -- F. Thin-layer chromatography -- G. Supercritical fluid chromatography -- H. Mass spectrometry -- I. Quality control -- CHAPTER 3 Lipid extraction, storage and sample handling -- A. Introduction -- B. Some practical considerations -- C. The principles of solvent extraction procedures -- D. Recommended procedures -- PART 2 Chromatographic separation and analysis of individual lipid classes -- CHAPTER 4 Analysis of simple lipid classes -- A. Preliminary fractionation of lipid extracts -- B. Low-pressure column chromatography -- C. Thin-layer chromatography (TLC) -- D. High-performance liquid chromatography -- E. Determination of lipid profiles by gas chromatography -- F. Analysis of specific simple lipid classes -- G. Oxidized fats and oils -- CHAPTER 5 Chromatographic analysis of phospholipids and glycosyldiacylglycerols -- A. Introduction -- B. Separation by low-pressure column chromatography -- C. Thin-layer chromatography -- D. High-performance liquid chromatography -- E. Quantification of complex lipids separated by chromatography -- F. Phosphorus nuclear magnetic resonance spectroscopy -- G. Some specific problems in the analysis of complex glycerolipids.
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CHAPTER 6 Chromatographic analysis of sphingolipids -- A. Introduction -- B. Preparation of sphingolipid-rich extracts -- C. Separation of neutral glycosphingolipid classes -- D. Separation of gangliosides -- E. Analysis of the hydrolysis products of glycosphingolipids -- F. Some miscellaneous glycolipids -- PART 3 The analysis of fatty acids -- CHAPTER 7 Preparation of derivatives of fatty acids -- A. Introduction -- B. Hydrolysis (saponification) of lipids -- C. The preparation of methyl and other esters of fatty acids -- D. Preparation of derivatives for mass spectrometry -- E. Derivatives of double bonds -- F. Derivatives of hydroxyl groups -- CHAPTER 8 Gas chromatographic analysis of fatty acid derivatives -- A. Introduction -- B. Columns and liquid phases -- C. Provisional identification using standards or retention time relationships -- D. Separation of the common fatty acids of plants and animals -- E. Positional and geometric isomers of unsaturated fatty acids -- F. Some applications to less common fatty acids -- G. Quantitative estimation of fatty acid compositions -- CHAPTER 9 Isolation of fatty acids and identification by spectroscopic and related techniques -- A. Introduction -- B. Simple concentration techniques -- C. Silver ion chromatography -- D. Reversed-phase high-performance liquid chromatography -- E. Adsorption chromatography -- F. Identification of fatty acids by chemical degradative procedures -- G. Spectroscopy of fatty acids -- H. Mass spectrometry of fatty acids -- PART 4 Chromatographic analysis of molecular species of lipids -- CHAPTER 10 Molecular species of triacylglycerols, diacylglycerols derived from complex lipids, and related lipids -- A. The nature of the problem -- B. Separation of triacylglycerol species -- C. Molecular species of monoand diacylglycerols -- D. Other simple lipids.
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CHAPTER 11 Chromatographic analysis of molecular species of intact phospholipids and glycolipids -- A. Introduction -- B. High-performance liquid chromatography separation of intact phospholipids -- C. Glycoglycerolipids -- D. Glycosphingolipids -- CHAPTER 12 Positional distributions of fatty acids in glycerolipids -- A. Introduction -- B. Positional distribution of fatty acids in triacylglycerols -- C. Enzymatic hydrolysis of complex lipids -- PART 5 Mass spectrometric analysis of lipids in lipidomics -- CHAPTER 13 Introduction to mass spectrometric analysis of lipids in lipidomics -- A. Lipidomics -- B. Brief history of mass spectrometry for lipid analysis -- C. Modern developments in mass spectrometry for lipid analysis -- D. Tandem mass spectrometry modes useful for lipid analyses in lipidomics -- E. Impact of recent developments related to mass spectrometric analyses of lipids in lipidomics -- CHAPTER 14 Characterization of lipids by electrospray ionization mass spectrometry -- A. Introduction -- B. Characterization of individual classes of phospholipids -- C. Characterization of individual classes of sphingolipids -- D. Characterization of simple glycerolipids and glycosyldiacylglycerols -- CHAPTER 15 Practical identification of individual lipid species in lipid extracts of biological samples -- A. Introduction -- B. LC-MS based identification of individual molecular species of lipid classes -- C. Identification of individual molecular species of lipid classes by shotgun lipidomics -- D. Location of double bonds in individual lipid species -- CHAPTER 16 Quantification of lipid molecular species by electrospray ionization mass spectrometry -- A. Introduction -- B. Internal standards and normalization -- C. Quantification of lipids by HPLC-coupled ESI-MS and ESI-MS/MS -- D. Quantification of lipids after direct infusion.
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E. Concerns associated with quantification -- F. Conclusions -- References -- Index.
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