In:
Clinical & Experimental Allergy, Wiley, Vol. 45, No. 10 ( 2015-10), p. 1542-1553
Abstract:
Although specific immunotherapy is the only aetiological treatment for allergic disorders, the underlying mechanisms are not fully understood. Specific immunotherapy induces changes in lymphocyte Th subsets from Th2 to Th1/Treg. Whether differences in immunological patterns underlie patient response to immunotherapy has not yet been established. Objectives We studied the immunological changes occurring during a 1‐year period of Dermatophagoides pteronyssinus ( DP ) immunotherapy and their relation with clinical outcome. Methods We included 34 patients with DP allergy who received subcutaneous specific immunotherapy ( SCIT ) for 1 year. Following treatment, patients were classified as responders or non‐responders. Fourteen allergic subjects who did not receive SCIT were included as controls. Peripheral blood was obtained at 0, 1, 3, 6 and 12 months and cultured with nD er p 1. Phenotypic changes, cytokine production and basophil response were analysed by flow cytometry; transcription factors were measured by mRNA quantification. Serum immunoglobulin levels were also measured. Results After 1 year of SCIT , 82% of cases showed improved symptoms (responders). Although increases in sIgG 4 were observed, BAT reactivity was not modified in these patients. Increases in T‐ BET / FOXP 3 as well as nD er p 1‐specific Th1/Treg frequencies were also observed, along with a decrease in Th2, Th9 and Th17. These changes corresponded to changes in cytokine levels. Conclusion Patients who respond well to DP ‐ SCIT show immunological differences compared to non‐responders. In responders, basal differences include a lower frequency of Th1 and higher frequencies of Th2, Th9 and Th17 cells. After 1 year of treatment, an increased production of sIgG 4 was observed in responders, along with a change in Th2 response towards Th1/Treg.
Type of Medium:
Online Resource
ISSN:
0954-7894
,
1365-2222
DOI:
10.1111/cea.2015.45.issue-10
Language:
English
Publisher:
Wiley
Publication Date:
2015
detail.hit.zdb_id:
2186232-1
detail.hit.zdb_id:
2004469-0
Permalink