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  • Alkalinity, total; Alkalinity, total, standard deviation; Aragonite saturation state; Bicarbonate ion; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; Calculated using CO2calc; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon/Nitrogen ratio; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Carbon per cell; Carbon per cell, standard deviation; Cell density; Cell density, standard deviation; Chlorophyll a; Chlorophyll a per cell; Chromista; Experiment; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Growth/Morphology; Growth rate; Heterosigma akashiwo; Hydrogen peroxide; Hydrogen peroxide per cell; Identification; Laboratory experiment; Laboratory strains; Nitrogen per cell; Nitrogen per cell, standard deviation; Not applicable; OA-ICC; Ocean Acidification International Coordination Centre; Ochrophyta; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH; pH, standard deviation; Phosphate; Phytoplankton; Potentiometric titration; Primary production/Photosynthesis; Primary production of carbon per chlorophyll a; Registration number of species; Salinity; Sample ID; Single species; Species; Spectrophotometric; Temperature, water; Treatment; Type; Uniform resource locator/link to reference  (1)
  • Microbial ecology  (1)
  • Ecogenomics
  • 2015-2019  (2)
Document type
Keywords
  • Alkalinity, total; Alkalinity, total, standard deviation; Aragonite saturation state; Bicarbonate ion; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; Calculated using CO2calc; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon/Nitrogen ratio; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Carbon per cell; Carbon per cell, standard deviation; Cell density; Cell density, standard deviation; Chlorophyll a; Chlorophyll a per cell; Chromista; Experiment; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Growth/Morphology; Growth rate; Heterosigma akashiwo; Hydrogen peroxide; Hydrogen peroxide per cell; Identification; Laboratory experiment; Laboratory strains; Nitrogen per cell; Nitrogen per cell, standard deviation; Not applicable; OA-ICC; Ocean Acidification International Coordination Centre; Ochrophyta; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH; pH, standard deviation; Phosphate; Phytoplankton; Potentiometric titration; Primary production/Photosynthesis; Primary production of carbon per chlorophyll a; Registration number of species; Salinity; Sample ID; Single species; Species; Spectrophotometric; Temperature, water; Treatment; Type; Uniform resource locator/link to reference  (1)
  • Microbial ecology  (1)
  • Ecogenomics
  • Nitrogen fixation  (2)
  • Amazon plume  (1)
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Year
  • 1
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    PANGAEA
    In:  Supplement to: Hennon, Gwenn M M; Williamson, Olivia M; Hernández Limón, María D; Haley, Sheean T; Dyhrman, Sonya T (2019): Non-linear Physiology and Gene Expression Responses of Harmful Alga Heterosigma akashiwo to Rising CO2. Protist, 170(1), 38-51, https://doi.org/10.1016/j.protis.2018.10.002
    Publication Date: 2024-05-22
    Description: Heterosigma akashiwo is a raphidophyte known for forming ichthyotoxic blooms. In order to predict the potential impacts of rising CO2 on H. akashiwo it is necessary to understand the factors influencing growth rates over a range of CO2 concentrations. Here we examined the physiology and gene expression response of H. akashiwo to concentrations from 200 to 1000 ppm CO2. Growth rate data were combined from this and previous studies and fit with a CO2 limitation-inhibition model that revealed an apparent growth optimum around 600–800 ppm CO2. Physiological changes included a significant increase in C:N ratio at 800 ppm CO2 and a significant decrease in hydrogen peroxide concentration at 1000 ppm. Whole transcriptome sequencing of H. akashiwo revealed sharp distinctions in metabolic pathway gene expression between 600 and 800 ppm CO2. Hierarchical clustering by co-expression identified groups of genes with significant correlations to CO2 and growth rate. Genes with significant differential expression with CO2 included carbon concentrating mechanism genes such as beta-carbonic anhydrases and a bicarbonate transporter, which may underpin shifts in physiology. Genes involved in cell motility were significantly changed by both elevated CO2 and growth rate, suggesting that future ocean conditions could modify swimming behavior in this species.
    Keywords: Alkalinity, total; Alkalinity, total, standard deviation; Aragonite saturation state; Bicarbonate ion; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; Calculated using CO2calc; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon/Nitrogen ratio; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Carbon per cell; Carbon per cell, standard deviation; Cell density; Cell density, standard deviation; Chlorophyll a; Chlorophyll a per cell; Chromista; Experiment; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Growth/Morphology; Growth rate; Heterosigma akashiwo; Hydrogen peroxide; Hydrogen peroxide per cell; Identification; Laboratory experiment; Laboratory strains; Nitrogen per cell; Nitrogen per cell, standard deviation; Not applicable; OA-ICC; Ocean Acidification International Coordination Centre; Ochrophyta; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH; pH, standard deviation; Phosphate; Phytoplankton; Potentiometric titration; Primary production/Photosynthesis; Primary production of carbon per chlorophyll a; Registration number of species; Salinity; Sample ID; Single species; Species; Spectrophotometric; Temperature, water; Treatment; Type; Uniform resource locator/link to reference
    Type: Dataset
    Format: text/tab-separated-values, 611 data points
    Location Call Number Limitation Availability
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  • 2
    Publication Date: 2022-05-25
    Description: Author Posting. © American Geophysical Union, 2015. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Global Biogeochemical Cycles 29 (2015): 1145–1164, doi:10.1002/2015GB005141.
    Description: Time-series observations are critical to understand the structure, function, and dynamics of marine ecosystems. The Hawaii Ocean Time-series program has maintained near-monthly sampling at Station ALOHA (22°45′N, 158°00′W) in the oligotrophic North Pacific Subtropical Gyre (NPSG) since 1988 and has identified ecosystem variability over seasonal to interannual timescales. To further extend the temporal resolution of these near-monthly time-series observations, an extensive field campaign was conducted during July–September 2012 at Station ALOHA with near-daily sampling of upper water-column biogeochemistry, phytoplankton abundance, and activity. The resulting data set provided biogeochemical measurements at high temporal resolution and documents two important events at Station ALOHA: (1) a prolonged period of low productivity when net community production in the mixed layer shifted to a net heterotrophic state and (2) detection of a distinct sea-surface salinity minimum feature which was prominent in the upper water column (0–50 m) for a period of approximately 30 days. The shipboard observations during July–September 2012 were supplemented with in situ measurements provided by Seagliders, profiling floats, and remote satellite observations that together revealed the extent of the low productivity and the sea-surface salinity minimum feature in the NPSG.
    Description: NOAA Climate Observation Division; National Science Foundation (NSF) Center for Microbial Oceanography: Research and Education (C-MORE) Grant Numbers: EF0424599, OCE-1153656, OCE-1260164; Gordon and Betty Moore Foundation Marine Microbiology Investigator
    Description: 2016-02-13
    Keywords: Primary productivity ; Microbial ecology ; Station ALOHA ; Temporal variability ; Biogeochemistry
    Repository Name: Woods Hole Open Access Server
    Type: Article
    Format: application/pdf
    Format: application/msword
    Location Call Number Limitation Availability
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