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  • 2005-2009  (19)
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  • 1
    Publication Date: 2024-03-12
    Description: 〈title xmlns:mml="http://www.w3.org/1998/Math/MathML"〉Abstract〈/title〉〈p xmlns:mml="http://www.w3.org/1998/Math/MathML" xml:lang="en"〉Iron (Fe) and manganese (Mn) availability and the divergent requirements of phytoplankton species were recently shown to be potential important drivers of Southern Ocean community composition. Knowledge about Antarctic phytoplankton species requirements for Fe and Mn remains, however, scarce. By performing laboratory experiments and additional calculations of the photosynthetic electron transport, we investigated the response of the ecologically important species 〈italic toggle="no"〉Phaeocystis antarctica〈/italic〉 under a combination of different Fe and Mn concentrations. Fe deprivation alone provoked typical physiological characteristics of Fe limitation in 〈italic toggle="no"〉P. antarctica〈/italic〉 (e.g., lowered growth and photosynthetic efficiency). In comparison, under Mn deprivation alone, the growth and carbon production of 〈italic toggle="no"〉P. antarctica〈/italic〉 were not impacted. Its tolerance to cope with low Mn concentrations resulted from an efficient photoacclimation strategy, including a higher number of active photosystems II through which fewer electrons were transported. This strategy allowed us to maintain similar high growth and carbon production rates as FeMn‐enriched cells. Due to its low Mn requirement, 〈italic toggle="no"〉P. antarctica〈/italic〉 performed physiologically as Fe‐deprived cells under the combined depletion of Fe and Mn. Hence, our study reveals that different from other Southern Ocean phytoplankton species, 〈italic toggle="no"〉P. antarctica〈/italic〉 possesses a high capacity to cope with natural low Mn concentrations, which can facilitate its dominance over others, potentially explaining its ecological success across the Southern Ocean.〈/p〉
    Description: Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659
    Description: https://doi.org/10.1594/PANGAEA.944462
    Keywords: ddc:577.7 ; Southern Ocean ; Antarctic phytoplankton ; Trace metal ; photophysiology ; carbon fixation
    Language: English
    Type: doc-type:article
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  • 2
    Publication Date: 2024-04-11
    Description: Ecophysiological studies looking at the combined effects of ocean acidification (OA) and iron (Fe) availability on Southern Ocean (SO) phytoplankton are still limited. To gain a better mechanistic understanding of how the two ecologically important SO phytoplankton groups cope with OA and Fe limitation, we conducted laboratory incubation experiments on the Antarctic cryptophyte Geminigera cryophila and the diatom Pseudo‐nitzschia subcurvata. Geminigera cryophila (CCMP 2564) was isolated from the Southern Ocean and obtained from Matt Johnson's Laboratory of Protistan Ecology at the Woods Hole Oceanography Institute, United States. Pseudo-nitzschia subcurvata was isolated from the Southern Ocean by P. Assmy during Polarstern expedition ANT- XXI/4. Both species were grown at 2°C under different pCO2 (400 vs. 900 μatm) and Fe (0.6 vs. 1.2 nM) conditions. For P. subcurvata, an additional high pCO2 level was applied (1400 μatm). For both species, growth, photophysiology, cellular quotas of particulate organic carbon, trace metals and pigments were assessed. Our study reveals that Fe limitation was detrimental for the growth of G. cryophila and suppressed the positive OA effect. The diatom was efficient in coping with low Fe, but was stressed by OA while both factors together strongly impacted its growth. The distinct physiological response of both species to OA and Fe limitation explains their occurrence in the field. Based on our results, Fe availability is an important modulator of OA effects on SO phytoplankton, with different implications on the occurrence of cryptophytes and diatoms in the future.
    Keywords: Alloxanthin; Alloxanthin, standard deviation; Carbon, organic, particulate; Carbon, organic, particulate, standard deviation; Carbon/Nitrogen ratio; Carbon/Nitrogen ratio, standard deviation; Chlorophyll a; Chlorophyll a, standard deviation; Chlorophyll c2; Chlorophyll c2, standard deviation; Cobalt/Carbon ratio; Cobalt/Carbon ratio, standard deviation; Connectivity between photosystem II; Connectivity between photosystem II, standard deviation; Copper/Carbon ratio; Copper/Carbon ratio, standard deviation; cryptophytes; culture experiment; Diadinoxanthin; Diadinoxanthin, standard deviation; diatoms; Electron transport rate, absolute; Electron transport rate, absolute, standard deviation; Elemental analyzer, HEKAtechGmbH, Euro EA; Fluorometer, fast repetition rate; FRRF; Fucoxanthin; Fucoxanthin, standard deviation; Functional absorption cross sections of photosystem II reaction centers; Functional absorption cross sections of photosystem II reaction centers, standard deviation; Functional photosystem II reaction centers; Functional photosystem II reaction centers, standard deviation; Growth rate, standard deviation; Inductively coupled plasma mass spectrometer (ICP-MS), Attom, Nu Instruments; Iron, cellular quota; Iron, cellular quota, standard deviation; Iron/Carbon ratio; Iron/Carbon ratio, standard deviation; Iron limitation; Irradiance; Laboratory experiment; Light microscopy (Utermöhl 1958); Light saturation point; Light saturation point, standard deviation; Light use efficiency; Manganese/Carbon ratio; Manganese/Carbon ratio, standard deviation; Maximal electron transport rate, standard deviation; Maximum light utilization efficiency, standard deviation; Maximum photochemical quantum yield of photosystem II; Maximum photochemical quantum yield of photosystem II, recovery; Maximum photochemical quantum yield of photosystem II, recovery, standard deviation; Maximum photochemical quantum yield of photosystem II, standard deviation; Nitrogen, organic, particulate; Non photochemical quenching; Non photochemical quenching, standard deviation; Ocean acidification; Particulate organic carbon, production, standard deviation; Particulate organic nitrogen production, standard deviation; Phytoplankton growth rate; Production of particulate organic carbon; Registration number of species; Reverse phase HPLC (High Performance Liquid Chromatography); Southern Ocean; Species; Treatment: dissolved iron; Treatment: partial pressure of carbon dioxide; Type of study; Uniform resource locator/link to reference; Zinc/Carbon ratio; Zinc/Carbon ratio, standard deviation
    Type: Dataset
    Format: text/tab-separated-values, 3068 data points
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  • 3
    Publication Date: 2024-02-28
    Description: The dataset shows the orginal data based on which grazing rates of micro- and nanozooplankton and the growth rates of their prey, in austral autumn (April) close to the Antarctic Peninsula in the SO, were calculated in Böckmann et al. (2024). The data was measured by dilution experiments. Besides the, in such experiments classically investigated chlorophyll a, particulate organic carbon, particulate organic nitrogen, abundances of picoplankton and nanoplankton as well as bacterial abundances were measured at three stations in the Bransfield Strait, Drake Passage and Scotia Sea. Samples were taken during PS112 from a depth of 25 meters, using a polyethylene line connected to an ALMATEC membrane pump, by careful (laminar flow, 3-6 liters per minute, bubble free bottle filling) and trace metal clean techniques, successfully used since 2014. The data was collected to investigate the importance that nano- and microzooplankton grazers have for the carbon cycle in the Southern Ocean.
    Keywords: Ammonium; ANT-XXXIII/3; Bacteria, high DNA; Bacteria, low DNA; Carbon, organic, particulate; Carbon, organic, particulate fractionated; Chlorophyll a; Continuous flow autoanalyzer, Alliance Instruments, Evolution III; Date/time end, experiment; Date/time start, experiment; Diatoms; dilution experiment; Dinoflagellates; Drake Passage; Elemental analyzer, HEKAtech, Euro Vector CHNS-O; Event label; Experimental treatment; Flow cytometer, BD Biosciences, BD Accuri C6; grazing rates; growth rates; In situ pump; Inverted light microscopy, Zeiss, Axio Observer D1; ISP; Laboratory experiment; Laboratory fluorometer, Turner, Trilogy; microzooplankton; Nanoeukaryotes; Nanoflagellates; nanozooplankton; Nitrate; Nitrite; Nitrogen, organic, particulate; Nitrogen, organic, particulate fractionated; Phosphate; Picoeukaryotes, fractionated; Polarstern; Population Shift and Ecosystem Response – Krill vs. Salps; POSER; PS112; PS112_106-1; PS112_26-1; PS112_61-3; Replicate; Sample code/label; Scotia Sea; Silicate; Southern Ocean; Type of study; WAP
    Type: Dataset
    Format: text/tab-separated-values, 953 data points
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  • 4
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    PANGAEA
    In:  Supplement to: Trimborn, Scarlett; Wolf-Gladrow, Dieter A; Richter, Klaus-Uwe; Rost, Björn (2009): The effect of pCO2 on carbon acquisition and intracellular assimilation in four marine diatoms. Journal of Experimental Marine Biology and Ecology, 376(1), 26-36, https://doi.org/10.1016/j.jembe.2009.05.017
    Publication Date: 2024-03-15
    Description: The effect of pCO2 on carbon acquisition and intracellular assimilation was investigated in the three bloom-forming diatom species, Eucampia zodiacus (Ehrenberg), Skeletonema costatum (Greville) Cleve, Thalassionema nitzschioides (Grunow) Mereschkowsky and the non-bloom-forming Thalassiosira pseudonana (Hust.) Hasle and Heimdal. In vivo activities of carbonic anhydrase (CA), photosynthetic O2 evolution, CO2 and HCO3? uptake rates were measured by membrane-inlet mass spectrometry (MIMS) in cells acclimated to pCO2 levels of 370 and 800 ?atm. To investigate whether the cells operate a C4-like pathway, activities of ribulose-1,5-bisphosphate carboxylase (RubisCO) and phosphoenolpyruvate carboxylase (PEPC) were measured at the mentioned pCO2 levels and a lower pCO2 level of 50 ?atm. In the bloom-forming species, extracellular CA activities strongly increased with decreasing CO2 supply while constantly low activities were obtained for T. pseudonana. Half-saturation concentrations (K1/2) for photosynthetic O2 evolution decreased with decreasing CO2 supply in the two bloom-forming species S. costatum and T. nitzschioides, but not in T. pseudonana and E. zodiacus. With the exception of S. costatum, maximum rates (Vmax) of photosynthesis remained constant in all investigated diatom species. Independent of the pCO2 level, PEPC activities were significantly lower than those for RubisCO, averaging generally less than 3%. All examined diatom species operate highly efficient CCMs under ambient and high pCO2, but differ strongly in the degree of regulation of individual components of the CCM such as Ci uptake kinetics and extracellular CA activities. The present data do not suggest C4 metabolism in the investigated species.
    Keywords: Alkalinity, Gran titration (Gran, 1950); Alkalinity, total; Aragonite saturation state; Bicarbonate ion; Bicarbonate uptake; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; Calculated; Calculated after Freeman & Hayes (1992); Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Chromista; EPOCA; Eucampia zoodiacus; EUR-OCEANS; European network of excellence for Ocean Ecosystems Analysis; European Project on Ocean Acidification; Experimental treatment; Extracellular carbonic anhydrase activity; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Intracellular carbonic anhydrase activity per chlorophyll a; Isotopic fractionation, during photosynthis; Laboratory experiment; Laboratory strains; Light:Dark cycle; Measured by loss of 18O (Silverman, 1982); Not applicable; OA-ICC; Ocean Acidification International Coordination Centre; Ochrophyta; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH; pH meter, WTW, pH 3000; Phytoplankton; Primary production/Photosynthesis; Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas; Radiation, photosynthetically active; Salinity; see reference(s); Single species; Skeletonema costatum; Species; SPP1158; Temperature, water; Thalassionema nitzschioides; Thalassiosira pseudonana
    Type: Dataset
    Format: text/tab-separated-values, 1263 data points
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  • 5
    Publication Date: 2024-05-13
    Description: Two Fe-Mn bottle amendment experiments with two natural phytoplankton communities were performed during Polarstern expedition PS97 in 2016 in the Drake Passage. At two locations, sea water was pumped (using trace metals clean techniques) from 25m depth and used to fill polycarbonate bottles after having passed through a cleaned 200 μm mesh (removing large grazers). The Control treatment was the sampled seawater without any trace metals addition while the other three treatments were enriched with either FeCl3 alone (0.5 nM; +Fe treatment) or MnCl2 alone (1 nM; +Mn treatment) or both trace metals together (+FeMn treatment). All treatments were done in triplicate 2,5L PC bottles. All incubation bottles were maintained at 30 μmol photons m-2 s-1 under a 16:8 (light:dark) hour cycle at 1 ̊C. Autotrophic picoeukaryotes were analyzed via flow cytometry. At the start and the end of the experiments, samples were preserved with 10% buffered formalin, flash-frozen in liquid nitrogen, and analyzed flow cytometrically to assess picoplankton densities. Before running the samples, 2 μL beads (Sperotech - Rainbow Fluorescent Particles (RFPs) - 2.11 μm) were added to each treatment as a size and fluorescence reference. Then picoeukaryotes were identified based on side scatter versus FL-3. Three P subgroups (0.2 – 2 μm) were differentiated according to their size : small (P1), medium (P2) and large (P3), according to sub-cluster of events.
    Keywords: ANT-XXXI/3; co-limitation; Drake Passage; Event label; Experiment; Experimental treatment; Flow cytometry; Incubation duration; Membrane pump; MP; Phytoplankton composition; Picoeukaryotes; Polarstern; Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas; PS97; PS97/043-1; PS97/087-4; Scotia Sea; Southern Ocean; SPP1158; trace elements
    Type: Dataset
    Format: text/tab-separated-values, 298 data points
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  • 6
    Publication Date: 2024-05-13
    Description: Determination of dissolved trace metals concentration: dFe and dMn concentrations were estimated from the initially sampled seawater. To this end, 100 mL of seawater were filtered through HCl-cleaned polycarbonate filters (0.2 μm pore size) using a TMC Nalgene filtration system and the filtrate was collected into PE bottle and stored triple bagged at 2 ̊C until analysis. Concentrations of the dFe and dMn were determined on a SeaFast system (Elemental Scientific, Omaha, NE, USA) coupled to an inductively coupled plasma mass spectrometer (ICP-MS, Element2, Thermo Fisher Scientific, resolution of R = 2000). During the pre-concentration step, an iminodiacetate (IDA) chelation column (part number CF-N-0200, Elemental Scientific) was used. The pre-filtered seawater samples were acidified to pH=1.7 with a double distilled nitric acid (HNO~3~) and were UV-treated using a 450 W photochemical UV power supply (ACE GLASS Inc., Vineland N. J., USA) to minimize adsorption of TMs onto the bottle walls and to reduce the formation of Mn and Fe hydroxides during storage. During each UV digestion step, two blanks were taken. The ICP-MS was optimized daily to achieve oxide forming rates below 0.3%. Each seawater sample was analyzed via standard addition to minimize any matrix effects, which might influence the quality of the analysis. To assess the accuracy and precision of the method, a NASS-7 (National Research Council of Canada) reference standard was analyzed in a 1:10 dilution (corresponding to environmentally representative concentrations) at the beginning, in the middle and at the end of each run (two batch runs; n = 18). The measured values were in the limits of the certified NASS-7 reference material, with a concentration of 351 ± 26 ng L^-1^ for dFe and 750 ± 60 ng L^-1^ for dMn (mean ± strandard deviation). The detection limits for Mn and Fe were 8.1 pM and 81.8 pM, respectively. Determination of the chlorophyll a fluorescence: For the 18 additional stations, chlorophyll a fluorescence measurements were collected using a Fast Repetition Rate Fluorometer (FRRf) coupled to a FastAct Laboratory system (FastOcean PTX), both from Chelsea Technologies Group. Samples were first dark acclimated for 1h before the meeasurement was perfomed.
    Keywords: ANT-XXXI/3; ANT-XXXIII/3; co-limitation; Date/Time of event; Drake Passage; Event label; Fluorometer, fast repetition rate; FRRF; GOFLO; Go-Flo bottles; ICP-MS, Elemental Scientific, seaFAST; In situ pump; Iron, dissolved; ISP; Latitude of event; Longitude of event; Manganese, dissolved; Membrane pump; Method/Device of event; MP; Photosynthetic efficiency; Phytoplankton composition; Polarstern; Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas; PS112; PS112_106-1; PS112_120-3; PS112_132-3; PS112_17-1; PS112_20-1; PS112_25-50; PS112_26-1; PS112_31-1; PS112_55-5; PS112_61-3; PS112_98-3; PS97; PS97/041-2; PS97/050-1; PS97/052-2; PS97/057-1; PS97/058-1; PS97/070-1; PS97/073-5; PS97/076-3; PS97/091-1; Scotia Sea; Southern Ocean; SPP1158; trace elements; Weddell Sea
    Type: Dataset
    Format: text/tab-separated-values, 59 data points
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  • 7
    Publication Date: 2024-05-13
    Description: Two Fe-Mn bottle amendment experiments with two natural phytoplankton communities were performed during Polarstern expedition PS97 in 2016 in the Drake Passage. At two locations, sea water was pumped (using trace metals clean techniques) from 25m depth and used to fill polycarbonate bottles after having passed through a cleaned 200 μm mesh (removing large grazers). The Control treatment was the sampled seawater without any trace metals addition while the other three treatments were enriched with either FeCl3 alone (0.5 nM; +Fe treatment) or MnCl2 alone (1 nM; +Mn treatment) or both trace metals together (+FeMn treatment). All treatments were done in triplicate 2,5L PC bottles. All incubation bottles were maintained at 30 μmol photons m-2 s-1 under a 16:8 (light:dark) hour cycle at 1 ̊C. Chlorophyll a samples were taken at the beginning and the end of both experiments. In order to compare the contribution of large (〉2 μm) relative to small cells (0.2-2 μm), 250 mL (on average) of samples were filtered onto 0.2 μm (for the total fraction) and 2 µm (for the large fraction) polycarbonate filters, hence the small fraction was calculated as the difference of the total and the large fraction. All samples were directly flash frozen into liquid nitrogen (N~2~) and then stored at −80 ̊C in the dark until further analysis. After being homogenized, samples were extracted in 90% acetone for 24h at 4 ̊C in the dark and analyzed fluorometrically on a Trilogy Fluorometer.
    Keywords: ANT-XXXI/3; Calculated; Chlorophyll a, size fraction 〉 2 µm; Chlorophyll a, size fraction 0.2-2 µm; Chlorophyll a, total; co-limitation; Drake Passage; Event label; Experiment; Experimental treatment; Incubation duration; Laboratory fluorometer, Turner, Trilogy; Membrane pump; MP; Phytoplankton composition; Polarstern; Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas; PS97; PS97/043-1; PS97/087-4; Scotia Sea; Southern Ocean; SPP1158; trace elements
    Type: Dataset
    Format: text/tab-separated-values, 164 data points
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  • 8
    Publication Date: 2024-05-13
    Description: This study highlights the importance of manganese (Mn) next to iron (Fe) for growth of specific Southern Ocean phytoplankton groups. Two Fe-Mn bottle amendment experiments with two natural phytoplankton communities were performed during Polarstern expedition PS97 in 2016 in the Drake Passage. At two locations, sea water was pumped (using trace metals clean techniques) from 25m depth and used to fill polycarbonate bottles after having passed through a cleaned 200 μm mesh (removing large grazers). The Control treatment was the sampled seawater without any trace metals addition while the other three treatments were enriched with either FeCl3 alone (0.5 nM; +Fe treatment) or MnCl2 alone (1 nM; +Mn treatment) or both trace metals together (+FeMn treatment). All treatments were done in triplicate 2,5L PC bottles. All incubation bottles were maintained at 30 μmol photons m-2 s-1 under a 16:8 (light:dark) hour cycle at 1 ̊C. After on average 15 days, samples for chlorophyll a content, flow cytometry and light macroscopy were taken in order to detect FeMn co-limitation effect on species composition. In addition to the two experiments, 9 in situ stations of PS97 were also sampled for dissolved Fe, dissolved Mn as well as photophysiology and to complete this dataset, data from PS112 (2018) were also used. The results showed that only some members of the phytoplankton community were Fe-Mn co-limited, with the biogeochemical important diatom group Fragilariopsis and one subgroup of picoeukaryotes.
    Keywords: co-limitation; Phytoplankton composition; Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas; Southern Ocean; SPP1158; trace elements
    Type: Dataset
    Format: application/zip, 4 datasets
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  • 9
    Publication Date: 2024-05-13
    Description: Two Fe-Mn bottle amendment experiments with two natural phytoplankton communities were performed during Polarstern expedition PS97 in 2016 in the Drake Passage. At two locations, sea water was pumped (using trace metals clean techniques) from 25m depth and used to fill polycarbonate bottles after having passed through a cleaned 200 μm mesh (removing large grazers). The Control treatment was the sampled seawater without any trace metals addition while the other three treatments were enriched with either FeCl3 alone (0.5 nM; +Fe treatment) or MnCl2 alone (1 nM; +Mn treatment) or both trace metals together (+FeMn treatment). All treatments were done in triplicate 2,5L PC bottles. All incubation bottles were maintained at 30 μmol photons m-2 s-1 under a 16:8 (light:dark) hour cycle at 1 ̊C. To determine the effects of the different treatments on the microplankton composition for the two Fe-Mn enrichment experiments, unfiltered seawater was collected at the start and the end of both experiments for later analysis via light microscopy in the home laboratory. Briefly, samples were fixed with hexamine-buffered formalin solution (2% final concentration) and Lugol's solution (1% final concentration) and stored at 2 °C in the dark until taxonomic analysis. All samples were allowed to settle in Utermöhl sedimentation chambers for at least 24 hours and were analyzed on an inverted light microscope, according to the method of Utermöhl (1958). Species were counted and identified according to taxonomic literature. Each aliquot was examined until at least 400 cells had been counted.
    Keywords: ANT-XXXI/3; Chaetoceros sp.; co-limitation; Drake Passage; Event label; Experiment; Experimental treatment; Fragilariopsis sp.; Incubation duration; Light microscopy (Utermöhl 1958); Membrane pump; MP; Phaeocystis antarctica; Phytoplankton composition; Polarstern; Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas; PS97; PS97/043-1; PS97/087-4; Pseudo-nitzschia sp.; Scotia Sea; Southern Ocean; SPP1158; trace elements
    Type: Dataset
    Format: text/tab-separated-values, 180 data points
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  • 10
    Publication Date: 2024-05-22
    Keywords: Alkalinity, total; Alkalinity, total, standard deviation; Alloxanthin; Alloxanthin, standard deviation; Aragonite saturation state; Bicarbonate ion; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbon, organic, particulate; Carbon, organic, particulate, standard deviation; Carbon/Nitrogen ratio; Carbon/Nitrogen ratio, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Chlorophyll a; Chlorophyll a, standard deviation; Chlorophyll c2; Chlorophyll c2, standard deviation; Chromista; Cobalt/Carbon ratio; Cobalt/Carbon ratio, standard deviation; Connectivity between photosystem II; Connectivity between photosystem II, standard deviation; Copper/Carbon ratio; Copper/Carbon ratio, standard deviation; Cryptophyta; Diadinoxanthin; Diadinoxanthin, standard deviation; Electron transport rate, absolute; Electron transport rate, absolute, standard deviation; Elemental analyzer, HEKAtechGmbH, Euro EA; Fluorometer, fast repetition rate; FRRF; Fucoxanthin; Fucoxanthin, standard deviation; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Functional absorption cross sections of photosystem II reaction centers; Functional absorption cross sections of photosystem II reaction centers, standard deviation; Functional photosystem II reaction centers; Functional photosystem II reaction centers, standard deviation; Geminigera cryophila; Growth/Morphology; Growth rate, standard deviation; Inductively coupled plasma mass spectrometer (ICP-MS), Attom, Nu Instruments; Inorganic toxins; Iron, cellular quota; Iron, cellular quota, standard deviation; Iron/Carbon ratio; Iron/Carbon ratio, standard deviation; Irradiance; Laboratory experiment; Laboratory strains; Light microscopy (Utermöhl 1958); Light saturation point; Light saturation point, standard deviation; Light use efficiency; Light use efficiency, standard deviation; Manganese/Carbon ratio; Manganese/Carbon ratio, standard deviation; Maximal electron transport rate; Maximal electron transport rate, standard deviation; Maximum photochemical quantum yield of photosystem II; Maximum photochemical quantum yield of photosystem II, recovery; Maximum photochemical quantum yield of photosystem II, recovery, standard deviation; Maximum photochemical quantum yield of photosystem II, standard deviation; Nitrogen, organic, particulate; Non photochemical quenching; Non photochemical quenching, standard deviation; Not applicable; OA-ICC; Ocean Acidification International Coordination Centre; Ochrophyta; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Particulate organic carbon, production, standard deviation; Particulate organic nitrogen production, standard deviation; Pelagos; pH; pH, standard deviation; Phosphate; Phytoplankton; Phytoplankton growth rate; Potentiometric; Potentiometric titration; Primary production/Photosynthesis; Production of particulate organic carbon; Pseudo-nitzschia subcurvata; Reverse phase HPLC (High Performance Liquid Chromatography); Salinity; Silicate; Single species; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Temperature, water; Treatment: dissolved iron; Treatment: partial pressure of carbon dioxide; Type; Zinc/Carbon ratio; Zinc/Carbon ratio, standard deviation
    Type: Dataset
    Format: text/tab-separated-values, 8948 data points
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