Description: The deep-sea mining industry is currently at a point where large-sale, commercial polymetallic nodule exploitation is becoming a more realistic scenario. At the same time, certain aspects such as the spatiotemporal scale of impacts, sediment plume dispersion and the disturbance-related biological responses remain highly uncertain. In this paper, findings from a small-scale seabed disturbance experiment in the German contract area (Clarion-Clipperton Zone, CCZ) are described, with a focus on the soft-sediment ecosystem component. Despite the limited spatial scale of the induced disturbance on the seafloor, this experiment allowed us to evaluate how short-term (〈 1 month) soft-sediment changes can be assessed based on sediment characteristics (grain size, nutrients and pigments) and metazoan meiofaunal communities (morphological and metabarcoding analyses). Furthermore, we show how benthic measurements can be combined with numerical modelling of sediment transport to enhance our understanding of meiofaunal responses to increased sedimentation levels. The lessons learned within this study highlight the major issues of current deep-sea mining-related ecological research such as deficient baseline knowledge, unrepresentative impact intensity of mining simulations and challenges associated with sampling trade-offs (e.g., replication).

Description: Abundance and composition of beach litter and microplastics (20–5000 μm, excluding fibres) were assessed in spring and autumn 2018 at various beaches along the Baltic Sea coast of Schleswig-Holstein, Northern Germany. The beach litter survey followed the OSPAR guidelines, while microplastics were extracted from sediment samples using density separation and were then identified with Raman μ-spectroscopy. We observed seasonality in the abundance and composition, but not in the mass of beach litter. The median microplastic abundance was 2 particles per 500 g of dry sediment in spring as well as in autumn, while six different synthetic polymers (PE, PP, PS, PET, PVC, POM) were detected. We found no correlation between the abundances of beach litter and microplastics. Our data represent the first systematic co-assessment of macro- and micro beach litter along the Baltic Sea coast of Schleswig-Holstein.

Description: Isolation and detection of microplastics (MP) in marine samples is extremely cost- and labor-intensive, limiting the speed and amount of data that can be collected. In the current work, we describe rapid measurement of net-collected MPs (net mesh size 300 µm) using a benchtop near-infrared hyperspectral imaging system during a research expedition to the subtropical North Atlantic gyre. Suspected plastic particles were identified microscopically and mounted on a black adhesive background. Particles were imaged with a Specim FX17 near-infrared linescan camera and a motorized stage. A particle mapping procedure was built on existing edge-finding algorithms and a polymer identification method developed using spectra from virgin polymer reference materials. This preliminary work focused on polyethylene, polypropylene, and polystyrene as they are less dense than seawater and therefore likely to be found floating in the open ocean. A total of 27 net tows sampled 2534 suspected MP particles that were imaged and analyzed at sea. Approximately 77.1% of particles were identified as polyethylene, followed by polypropylene (9.2%). A small fraction of polystyrene was detected only at one station. Approximately 13.6% of particles were either other plastic polymers or were natural materials visually misidentified as plastics. Particle size distributions for PE and PP particles with a length greater than 1 mm followed an approximate power law relationship with abundance. This method allowed at-sea, near real-time identification of MP polymer types and particle dimensions, and shows great promise for rapid field measurements of microplastics in net-collected samples.

Description: Abyssal plain communities rely on the overlying water column for a settling flux of organic matter. The origin and rate of this flux as well as the controls on its fine-scale spatial distribution following seafloor settlement are largely unquantified. This is particularly true across regions where anthropogenically-induced seafloor disturbance has occurred. Here, we observed, quantified and mapped a mass deposition event of gelatinous zooplankton carcasses (pyrosomes) in July-September 2015 across one such physically disturbed region in the Peru Basin polymetallic nodule province (4150 m). Seafloor in this area was disturbed with a plough harrow in 1989 (as part of the DISCOL experiment) causing troughs in the sediment. Other parts were disturbed with an epibenthic sled (EBS) during a cruise in 2015 resulting in steep-walled, U-shaped troughs. We investigated two hypotheses: a) gelatinous food falls contribute significantly to the abyssal plain carbon pump and b) physical seafloor disturbance influences abyssal distribution of organic matter. We combined optical and bathymetric seafloor observations, to analyze pyrosome distribution on seabeds with different levels of disturbance. 2954 pyrosome colonies and associated taxa were detected in 〉 14,000 seafloor images. The mean regional carbon (C) deposition associated with pyrosome carcasses was significant compared to the flux of particulate organic C (182 to 1543%), and the total respired benthic C flux in the DISCOL Experimental Area (39 to 184%). EBS-disturbed seafloor tracks contained 72 times more pyrosome-associated C than an undisturbed reference site, and up to 4 times more than an area disturbed in 1989. Deposited pyrosomes collected had a higher proportion of labile fatty acids compared to the sediment. We document the temporal and spatial extent of an abyssal food fall event with unprecedented detail and show that physical seafloor disturbance results in the accumulation of detrital material. Such accumulation may reduce oxygen availability and alter benthic community structure. Understanding both the relevance of large food falls and the fine scale topography of the seafloor, is necessary for impact assessment of technologies altering seafloor integrity (e.g. as a result of bottom-trawling or deep seabed mining) and may improve their management on a global scale.

Description: Highlights • This study simulates the sedimentation-driven development of multiple stacked BSRs in the Danube paleo-delta, Black Sea. • Formation of multiple BSRs in the Black Sea is controlled by the sequence of sedimentation events of the levees induced by sea-level changes. • Kinetics of phase transitions plays a key role in the coexistence, location, and timing of the multiple BSRs. • Development of multiple stacked BSRs is possible only under a narrow range of parameters, unique for the Danube delta setting. Abstract The gas hydrate stability zone (GHSZ) is defined by pressure-temperature-salinity (pTS) constraints of natural gas hydrate (GH) system. It refers to a depth interval which usually extends several hundred meters into the sediment column at sufficient water depths. The lower boundary of the GHSZ often coincides in seismic reflection data with a bottom simulating reflector (BSR), which indicates the transition between the underlying free gas and the overlying no-free gas zone at the thermodynamic stability boundary. The GHSZ in geological systems is dynamic and can shift in response to sedimentation processes and/or changes in environmental conditions such as bottom water temperatures, hydrostatic pressure, and water salinity. The appearance of multiple BSRs has been interpreted as remnants of former GHSZ shifts which have persisted over geological timescales. In this study, we numerically simulate the sedimentation-driven development of multiple stacked BSRs in the Danube deep-sea fan in the Black Sea. We show that in this dynamic sediment depositional regime sufficient amounts of residual gas remain trapped in the former GHSZ, given sufficiently high initial gas hydrate saturations, so that paleo-BSRs could persist over long time scales (similar to 300 kyr). In particular, the formation and persistence of multiple BSRs in the Danube Delta is controlled by the sequence of sedimentation events of the levees induced by sea-level change. The kinetics of methane phase transitions between gas hydrate, dissolved methane, and free gas plays a key role in the coexistence, location and timing of the multiple BSRs. Thus, For a given permeability, distinct multiple BSRs appear only for a narrow range of GH formation (10(-14) 〈 k(f) [mol/m(2) Pa s] 〈= 10(-12)) and dissociation rates (10(-16) 〈 k(d) [mol/m(2) Pa s] 〈 10(-14)).

Description: The impact of oxygen on the preservation of organic matter in marine surface sediments is still controversial. We revisited this long-standing debate by determining the burial efficiency of sedimentary organic matter in the Black Sea, the largest anoxic and euxinic basin in the modern ocean. Surface sediments were sampled in the Danube paleodelta on the northwestern margin of the Black Sea at 420–1550 m water depth. Steady-state modeling of solid species (particulate organic carbon and nitrogen) and solutes (ammonium, sulfate, and total alkalinity) in sediments was performed to quantify rates of mass accumulation, particulate organic matter (POM) degradation, and POM burial. We develop a novel analytical model to quantify these rates applying an inverse modelling approach to down core data accounting for molecular diffusion, sediment burial and compaction. Our model results indicate that 56.7 ± 6.6 % of the particulate organic matter deposited in the study area is not degraded in surface sediments but accumulates below 10 cm sediment depth. This burial efficiency is substantially higher than those previously derived for seafloor areas underlying oxygenated bottom waters. Hence, our study confirms previous studies showing that euxinic bottom water conditions promote the preservation of particulate organic matter in marine sediments.

Description: Following several small-scale benthic disturbance experiments, an industrial polymetallic nodule collector trial was conducted by the company Global Sea mineral Resources (GSR) in their exploration contract area in the Clarion-Clipperton Zone using the pre-prototype vehicle Patania II (PATII). In this study, meiofaunal (i.e., nematode abundance, ASV diversity and genus composition) and environmental (i.e., grain size, total organic carbon/total nitrogen and pigment) properties are compared between disturbance categories (i.e., Pre-impact, Collector Impact and Plume Impact). One week after the trial, proxies for food availability within the Collector Impact sediments were altered with lower total organic carbon (TOC) and pigment (i.e., CPE: sum of Chlorophyll a and phaeopigments) values. Albeit not significant, the observed decrease of nematode abundance and ASV diversity, further indicate the consequences of the removal of the ecologically important surface sediment layer within the PATII tracks. Next to sediment removal, exposed sediments were modified in different ways (e.g., central strips, parallel caterpillar imprints with alternating bands of depressions/ripples and interface patches) and were also subject to heavy collector-induced sediment blanketing. We propose that these cumulative impacts have led to intricate seabed modifications with various levels of disturbance intensity which resulted in the high meiofaunal variability observed. Adjacent nodule-rich areas (i.e., Plume Impact) received considerable levels of sediment deposition (2-3 cm) and were defined by significantly lower food sources (CPE, TOC, carbon to nitrogen ratio) and an observation of meiofaunal enrichment (i.e., higher average nematode abundance and ASV diversity; although statistically non-significant), but mechanisms behind these ecological changes (e.g., suspended material-surface fluxes, passive dispersal of fauna in the plume vs. active upward migration and “viability” of redeposited fauna) remain unresolved. We conclude that complex benthic pressure-response relationships associated with the PATII trial, combined with the high degree of natural spatial and temporal variability in abyssal meiofaunal communities and sedimentary parameters, complicates the quantitative assessment of deep-sea mining associated disturbances.

Description: A stratigraphic complex composed of mass transport deposits (MTDs), where the gas occurrence allows for the formation of a gas chimney and pipe structure, is identified based on seismic interpretation in the QiongDongNan area of the northern South China Sea. During the Fifth Gas Hydrate Drilling Expedition of the Guangzhou Marine Geological Survey, this type of complex morphology that has close interaction with local gas hydrate (GH) distribution was eventually confirmed. A flow-reaction model is built to explore the spatial–temporal matching evolution process of massive GH reservoirs since 30 kyr before the present (BP). Five time snapshots, including 30, 20, 10, and 5 kyr BP, as well as the present, have been selected to exhibit key strata-evolving information. The results of in situ tensile estimation imply fracturing emergence occurs mostly at 5 kyr BP. Six other environmental scenarios and three cases of paleo-hydrate existence have been compared. The results almost coincide with field GH distribution below the bottom MTD from drilling reports, and state layer fracturing behaviors always feed and probably propagate in shallow sediments. It can be concluded that this complex system with 10% pre-existing hydrates results in the exact distribution and occurrence in local fine-grained silty clay layers adjacent to upper MTDs.