Electronic Resource
Oxford, UK
:
Blackwell Publishing Ltd
Journal of cutaneous pathology
21 (1994), S. 0
ISSN:
1600-0560
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Normal skin was cryoprotected by submerging it in a mixture of 30% dimethylformamide (DMF) in PBS or RPMI. Subsequently it was frozen in liquid propane gas. Cryosubstitution was carried out at −90°C by using methanol to which uranyl acetate or osmium tetroxide were added. The tissue was embedded in either Lowicryl K4M at −40°C or in Epon at +60°C. The- tissue was evaluated by its overall preservation of ultras-tructural details and by its labeling intensity alter incubation with either anti-clesmoglein or anti-type VII collagen monoclonal antibodies. The mixture of DMF and PBS caused an electron-dense precipitate within the cell. The overall morphology was better in Epon-embedded material than in K4M-embedclecl material. However, the labeling was best in K4M material. Regardless of whether the tissue was embedded in Epon or K4M, the addition of osmium tetroxide markedly reduced the degree of labeling.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1600-0560.1994.tb00708.x
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