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  • 1990-1994  (7)
  • 1991  (7)
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  • 1990-1994  (7)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Relationship between fermentation capability and fatty acid composition of free and immobilized Saccharomyces cerevisiae (1991)
    Springer
    Applied microbiology and biotechnology 34 (1991), S. 502-508 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The fatty acid composition of Saccharomyces cerevisiae immobilized by entrapment in calcium-alginate beads or adsorption on sintered glass was compared to that of freely suspended cells under different fermentation conditions. The fermentation product ethanol was found to cause a shift towards saturation in the fatty acid composition under anaerobic conditions. Immobilized cells contained significantly higher percentages of saturated fatty acyl residues, especially of palmitic acid (16:0), and a decreased amount of oleic acid (18:1) compared with free cells. The percentage saturation of total fatty acid composition correlates positively with improved fermentation rates obtained with the immobilized cells. This enhanced saturation of fatty acid composition in immobilized cells may be due to altered osmotic conditions in the microenvironment of the cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00180578
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Physiology of polyol formation by free and immobilized cells of the osmotolerant yeast Pichia farinosa (1991)
    Springer
    Applied microbiology and biotechnology 35 (1991), S. 258-263 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Some physiological data of cells of Pichia farinosa immobilized on sintered glass Raschig rings were compared with data from free cells. Glucose consumption and productivity of total polyols (arabitol, glycerol and erythritol) showed a simultaneous “inter-lag” phase. Enzymes that catalyse steps of the pentosephosphate pathway (glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, transaldolase and polyol dehydrogenase) showed a distinct increase after transfer of the cells into production medium. The activity of glycerol-3-phosphate dehydrogenase was generally low. Only alcohol dehydrogenase presented the inter-lag phase mentioned above.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00184698
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Biodegradation of 4-chlorophenol by adsorptive immobilized Alcaligenes sp. A 7-2 in soil (1991)
    Springer
    Applied microbiology and biotechnology 35 (1991), S. 662-668 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Alcaligenes sp. A 7-2 immobilized on granular clay has been applied in a percolator to degrade 4-chlorophenol in sandy soil. Good adsorption rates on granular clay were achieved using cell suspensions with high titres and media at pH 8.0. The influence of various parameters such as aeration rate, pH, temperature, concentration of 4-chlorophenol and size of inoculum on the degradation rate were investigated. During fedbatch fermentations under optimal culture conditions, concentrations of 4-chlorophenol up to 160 mg·1−1 could be degraded. Semicontinuous culture experiments demonstrated that the degradation potential in soil could be well established and enhanced by the addition of immobilized bacteria. Continuous fermentation was performed with varying 4-chlorophenol concentrations in the feed and different input levels. The maximum degradation rate was 1.64 g·1−1·day−1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00169634
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Aspects of biotechnology in a united Germany (mini-review) (1991)
    Springer
    Applied microbiology and biotechnology 35 (1991), S. 699-699 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00169641
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Biodegradation of phenanthrene by Arthrobacter polychromogenes isolated from a contaminated soil (1991)
    Springer
    Applied microbiology and biotechnology 34 (1991), S. 804-808 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Degradation of phenanthrene by Arthrobacter polychromogenes isolated from a contaminated soil was investigated. In experiments in which [9-14C]-phenanthrene was incubated with cultures of A. polychromogenes containing 150 mg phenanthrene/l it was shown that after 26 h of incubation 47.7% of the recovered radiolabelled carbon originally present was metabolized to 14CO2, 47.8% was recovered from the aqueous fraction, and 4.5% remained in the dichloromethane fraction. Increasing phenanthrene concentration in the culture medium resulted in improved growth and degradation rates, probably due to the higher amount of phenanthrene crystals in the medium. Shifting the temperature from 30°C to 35°C did not influence phenanthrene degradation significantly but inhibited cell division of A. polychromogenes. Medium supplementation with glucose led to stimulation of phenanthrene degradation at low amounts of glucose (0.45 g/l) whereas at higher concentrations (3 g/l) phenanthrene mineralization decreased.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00169354
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Aspects of biotechnology in a united Germany (1991)
    Springer
    Applied microbiology and biotechnology 35 (1991), S. 135-143 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00184676
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Increase of phenol tolerance of Escherichia coli by alterations of the fatty acid composition of the membrane lipids (1991)
    Springer
    Archives of microbiology 157 (1991), S. 49-53 
    Details
    ISSN: 1432-072X
    Keywords: Phenol ; 4-Chlorophenol ; p-Cresol ; Fatty acids ; Lipids ; Supplementation ; Tolerance ; Membrane ; E. coli
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In the presence of sublethal concentrations of phenol, 4-chlorophenol, and p-cresol in the growth medium, cells of Escherichia coli modified the fatty acid composition of their lipids. The result of these changes was an increase in the degree of saturation of lipids probably in order to compensate an increase of fluidity of the membrane induced by the phenols. Supplementation of the growth medium with saturated fatty acids could also enhance the degree of lipid saturation due to the incorporation of the acyl chains in the phospholipids. At the same time the growth of cells was less inhibited than in unsupplemented cells. The increase of tolerance of cells by manipulating the lipid composition indicates that the membrane structure plays a crucial role in the mode of action of phenols.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00245334
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    Paper (German National Licenses)
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