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  • 1
    Online Resource
    Online Resource
    Protamine 1 as a secreted colorectal cancer-specific antigen facilitating G1/S phase transition under nutrient stress conditions
    Springer Science and Business Media LLC ; 2023
    In:  Cellular Oncology Vol. 46, No. 2 ( 2023-04), p. 357-373
    Details
    In: Cellular Oncology, Springer Science and Business Media LLC, Vol. 46, No. 2 ( 2023-04), p. 357-373
    Abstract: Cancer testis antigens (CTAs) are optimal tumor diagnostic markers and involved in carcinogenesis. However, colorectal cancer (CRC) related CTAs are less reported with impressive diagnostic capability or relevance with tumor metabolism rewiring. Herein, we demonstrated CRC-related CTA, Protamine 1 (PRM1), as a promising diagnostic marker and involved in regulation of cellular growth under nutrient deficiency. Methods Transcriptomics of five paired CRC tissues was used to screen CRC-related CTAs. Capability of PRM1 to distinguish CRC was studied by detection of clinical samples through enzyme linked immunosorbent assay (ELISA). Cellular functions were investigated in CRC cell lines through in vivo and in vitro assays. Results By RNA-seq and detection in 824 clinical samples from two centers, PRM1 expression were upregulated in CRC tissues and patients` serum. Serum PRM1 showed impressive accuracy to diagnose CRC from healthy controls and benign gastrointestinal disease patients, particularly more sensitive for early-staged CRC. Furthermore, we reported that when cells were cultured in serum-reduced medium, PRM1 secretion was upregulated, and secreted PRM1 promoted CRC growth in culture and in mice. Additionally, G1/S phase transition of CRC cells was facilitated by PRM1 protein supplementation and overexpression via activation of PI3K/AKT/mTOR pathway in serum deficient medium. Conclusions In general, our research presented PRM1 as a specific CRC antigen and illustrated the importance of PRM1 in CRC metabolism rewiring. The new vulnerability of CRC cells was also provided with the potential to be targeted in future. Graphical abstract Diagnostic value and grow factor-like biofunction of PRM1 A represents the secretion process of PRM1 regulated by nutrient deficiency. B represents activation of PI3K/AKT/mTOR pathway of secreted PRM1.
    Type of Medium: Online Resource
    ISSN: 2211-3428 , 2211-3436
    URL: Article
    DOI: 10.1007/s13402-022-00754-w
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2023
    detail.hit.zdb_id: 2595105-1
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  • 2
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    Online Resource
    Image4.JPEG
    Frontiers Media SA ; 2022
    In:  Frontiers in Cell and Developmental Biology Vol. 10 ( 2022-1-31)
    Details
    In: Frontiers in Cell and Developmental Biology, Frontiers Media SA, Vol. 10 ( 2022-1-31)
    Abstract: Objective: Colon cancer is one of the most frequent and lethal neoplasias. Altered metabolic activity is a well-known hallmark for cancer. The present study is aiming to screen key genes associated with tumor metabolism and construct a prognostic signature of colon cancer patients. Methods: Glutamine- and UC- metabolism related genes were downloaded from GSEA MsigDB. Three key genes were screened by Cox regression analysis with data samples downloaded from TCGA and GSE29623 database. Consistent clustering based on the prognostic genes identified was employed to divide the colon cancer samples into two clusters with significant OS differences. The mRNA and protein expression of the key genes in colon tissues and matched adjacent noncancerous tissues of 16 patients were detected by IHC, qPCR, and Western blot to validate the constructed clustering model. GO, GSVA, and IPA were used to predict the relevant metabolic pathways. Results: According to the three key genes identified, i.e., ASNS, CEBPA, and CAD, the cohort can be divided into two clusters with prognosis differences. Clinical specimen results confirmed that the risk model established was effective, and the different expression pattern of ASNS and CEBPA was correlated with TNM stage and lymph node metastasis, whilst that of CAD was correlated with post-operative tumor metastasis and recurrence. Molecular mechanism prediction indicated that CREB, insulin, and RNA Pol II were the key nodes affecting CEBPA and ASNS expression. Moreover, TIDE algorithm reflected the better immune response of the cluster with shorter OS. Further immune infiltration and checkpoints analyses provided important reference for clinicians to perform individualized immunotherapy. Conclusion: Differential expression profile of three aspartic acid metabolic-associated genes, ASNS, CEBPA, and CAD, can be considered as a risk model with a good evaluation effect on the prognosis of colon cancer patients.
    Type of Medium: Online Resource
    ISSN: 2296-634X
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    DOI: 10.3389/fcell.2022.812271
    DOI: 10.3389/fcell.2022.812271.s001
    DOI: 10.3389/fcell.2022.812271.s002
    DOI: 10.3389/fcell.2022.812271.s003
    DOI: 10.3389/fcell.2022.812271.s004
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2022
    detail.hit.zdb_id: 2737824-X
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  • 3
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    Online Resource
    Crystal structure of dimethyl 5-(benzylamino)isophthalate, C 17 H 17 NO 4
    Walter de Gruyter GmbH ; 2016
    In:  Zeitschrift für Kristallographie - New Crystal Structures Vol. 231, No. 3 ( 2016-9-1), p. 987-988
    Details
    In: Zeitschrift für Kristallographie - New Crystal Structures, Walter de Gruyter GmbH, Vol. 231, No. 3 ( 2016-9-1), p. 987-988
    Abstract: C 17 H 17 NO 4 , monoclinic, C2/ c (no. 15), a = 24.367(3) Å, b = 6.2556(6) Å, c = 20.854(2) Å, β = 110.016(2)°, V = 2986.9(5) Å 3 , Z = 8, R gt (F) = 0.0539, wR ref ( F 2 ) = 0.1578, T = 293(2) K.
    Type of Medium: Online Resource
    ISSN: 2197-4578 , 1433-7266
    URL: Article
    DOI: 10.1515/ncrs-2016-0039
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2016
    detail.hit.zdb_id: 2039576-0
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  • 4
    Online Resource
    Online Resource
    Role of glutamine and its metabolite ammonia in crosstalk of cancer-associated fibroblasts and cancer cells
    Springer Science and Business Media LLC ; 2021
    In:  Cancer Cell International Vol. 21, No. 1 ( 2021-09-09)
    Details
    In: Cancer Cell International, Springer Science and Business Media LLC, Vol. 21, No. 1 ( 2021-09-09)
    Abstract: Cancer-associated fibroblasts (CAFs), the most abundant cells in the tumor microenvironment, play an indispensable role in cancer initiation, progression, metastasis, and metabolism. The limitations of traditional treatments can be partly attributed to the lack of understanding of the role of the tumor stroma. For this reason, CAF targeting is gradually gaining attention, and many studies are trying to overcome the limitations of tumor treatment with CAF as a breakthrough. Glutamine (GLN) has been called a “nitrogen reservoir” for cancer cells because of its role in supporting anabolic processes such as fuel proliferation and nucleotide synthesis, but ammonia is a byproduct of the metabolism of GLN and other nitrogenous compounds. Moreover, in some studies, GLN has been reported as a fundamental nitrogen source that can support tumor biomass. In this review, we discuss the latest findings on the role of GLN and ammonia in the crosstalk between CAFs and cancer cells as well as the potential therapeutic implications of nitrogen metabolism.
    Type of Medium: Online Resource
    ISSN: 1475-2867
    URL: Article
    DOI: 10.1186/s12935-021-02121-5
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2021
    detail.hit.zdb_id: 2091573-1
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  • 5
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    Online Resource
    〈p〉Lnc HAGLR Promotes Colon Cancer Progression Through Sponging miR‐185‐5p and Activating CDK4 and CDK6 in vitro and in vivo〈/p〉
    Informa UK Limited ; 2020
    In:  OncoTargets and Therapy Vol. Volume 13 ( 2020-06), p. 5913-5925
    Details
    In: OncoTargets and Therapy, Informa UK Limited, Vol. Volume 13 ( 2020-06), p. 5913-5925
    Type of Medium: Online Resource
    ISSN: 1178-6930
    URL: Article
    DOI: 10.2147/OTT.S246092
    Language: English
    Publisher: Informa UK Limited
    Publication Date: 2020
    detail.hit.zdb_id: 2495130-4
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