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  • 1
    In: Scientific Reports, Springer Science and Business Media LLC, Vol. 13, No. 1 ( 2023-01-20)
    Abstract: The connection between Pediatric Inflammatory Multisystem Syndrome (PIMS) and Kawasaki Disease (KD) is not yet fully understood. Using the same national registry, clinical features and outcome of children hospitalized in Germany, and Innsbruck (Austria) were compared. Reported to the registry were 395 PIMS and 69 KD hospitalized patients. Patient age in PIMS cases was higher than in KD cases (median 7 [IQR 4–11] vs. 3 [IQR 1–4] years). A majority of both PIMS and KD patients were male and without comorbidities. PIMS patients more frequently presented with organ dysfunction, with the gastrointestinal (80%), cardiovascular (74%), and respiratory (52%) systems being most commonly affected. By contrast, KD patients more often displayed dermatological (99% vs. 68%) and mucosal changes (94% vs. 64%), plus cervical lymph node swelling (51% vs. 34%). Intensive care admission (48% vs. 19%), pulmonary support (32% vs. 10%), and use of inotropes/vasodilators (28% vs. 3%) were higher among PIMS cases. No patients died. Upon patient discharge, potentially irreversible sequelae—mainly cardiovascular—were reported (7% PIMS vs. 12% KD). Despite differences in age distribution and disease severity, PIMS and KD cases shared many common clinical and prognostic characteristics. This supports the hypothesis that the two entities represent a syndrome continuum.
    Type of Medium: Online Resource
    ISSN: 2045-2322
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2023
    detail.hit.zdb_id: 2615211-3
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  • 2
    Online Resource
    Online Resource
    Springer Science and Business Media LLC ; 2017
    In:  Climate Dynamics Vol. 49, No. 11-12 ( 2017-12), p. 3851-3876
    In: Climate Dynamics, Springer Science and Business Media LLC, Vol. 49, No. 11-12 ( 2017-12), p. 3851-3876
    Type of Medium: Online Resource
    ISSN: 0930-7575 , 1432-0894
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2017
    detail.hit.zdb_id: 382992-3
    detail.hit.zdb_id: 1471747-5
    SSG: 16,13
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  • 3
    In: Genomics, Proteomics & Bioinformatics, Oxford University Press (OUP), Vol. 20, No. 2 ( 2022-04-01), p. 405-417
    Abstract: High-quality DNA extraction is a crucial step in metagenomic studies. Bias by different isolation kits impairs the comparison across datasets. A trending topic is, however, the analysis of multiple metagenomes from the same patients to draw a holistic picture of microbiota associated with diseases. We thus collected bile, stool, saliva, plaque, sputum, and conjunctival swab samples and performed DNA extraction with three commercial kits. For each combination of the specimen type and DNA extraction kit, 20-gigabase (Gb) metagenomic data were generated using short-read sequencing. While profiles of the specimen types showed close proximity to each other, we observed notable differences in the alpha diversity and composition of the microbiota depending on the DNA extraction kits. No kit outperformed all selected kits on every specimen. We reached consistently good results using the Qiagen QiAamp DNA Microbiome Kit. Depending on the specimen, our data indicate that over 10 Gb of sequencing data are required to achieve sufficient resolution, but DNA-based identification is superior to identification by mass spectrometry. Finally, long-read nanopore sequencing confirmed the results (correlation coefficient & gt; 0.98). Our results thus suggest using a strategy with only one kit for studies aiming for a direct comparison of multiple microbiotas from the same patients.
    Type of Medium: Online Resource
    ISSN: 1672-0229 , 2210-3244
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2022
    detail.hit.zdb_id: 2233708-8
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  • 4
    In: Engineering in Life Sciences, Wiley, Vol. 17, No. 5 ( 2017-05), p. 585-593
    Abstract: Near infrared spectroscopy is a rapid and nondestructive method for compositional analysis of biological material. The technology is widely used within bioreactors and possesses potential as a standardized method for quality control in miniaturized microfluidic cell culture systems. Here, we established a method for quantification of cell density and viability of adherent HepaRG cells cultured in a translucent, miniaturized cell culture biochip. The newly developed statistical models for interpretation of near infrared spectroscopy from biochips are the basis for a novel method of fast, continuous, and contact‐free analysis of cell viability and real‐time monitoring of cell growth. The technique thus paves the way for a robust and reliable high‐throughput analysis of biochip‐embedded cell cultures.
    Type of Medium: Online Resource
    ISSN: 1618-0240 , 1618-2863
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2017
    detail.hit.zdb_id: 2071199-2
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  • 5
    Online Resource
    Online Resource
    American Chemical Society (ACS) ; 2018
    In:  The Journal of Physical Chemistry B Vol. 122, No. 38 ( 2018-09-27), p. 8846-8852
    In: The Journal of Physical Chemistry B, American Chemical Society (ACS), Vol. 122, No. 38 ( 2018-09-27), p. 8846-8852
    Type of Medium: Online Resource
    ISSN: 1520-6106 , 1520-5207
    RVK:
    Language: English
    Publisher: American Chemical Society (ACS)
    Publication Date: 2018
    detail.hit.zdb_id: 1357799-2
    detail.hit.zdb_id: 2006039-7
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  • 6
    In: Geoscientific Model Development, Copernicus GmbH, Vol. 15, No. 22 ( 2022-11-25), p. 8613-8638
    Abstract: Abstract. While advanced computational capabilities have enabled the development of complex ocean general circulation models (OGCMs) for marginal seas, systematic comparisons of regional ocean models and their setups are still rare. The Baltic Sea Model Intercomparison Project (BMIP), introduced herein, was therefore established as a platform for the scientific analysis and systematic comparison of Baltic Sea models. The inclusion of a physically consistent regional reanalysis data set for the period 1961–2018 allows for standardized meteorological forcing and river runoff. Protocols to harmonize model outputs and analyses are provided as well. An analysis of six simulations performed with four regional OGCMs differing in their resolution, grid coordinates, and numerical methods was carried out to explore intermodel differences despite harmonized forcing. Uncertainties in the modeled surface temperatures were shown to be larger at extreme than at moderate temperatures. In addition, a roughly linear increase in the temperature spread with increasing water depth was determined and indicated larger uncertainties in the near-bottom layer. On the seasonal scale, the model spread was larger in summer than in winter, likely due to differences in the models' thermocline dynamics. In winter, stronger air–sea heat fluxes and vigorous convective and wind mixing reduced the intermodel spread. Uncertainties were likewise reduced near the coasts, where the impact of meteorological forcing was stronger. The uncertainties were highest in the Bothnian Sea and Bothnian Bay, attributable to the differences between the models in the seasonal cycles of sea ice triggered by the ice–albedo feedback. However, despite the large spreads in the mean climatologies, high interannual correlations between the sea surface temperatures (SSTs) of all models and data derived from a satellite product were determined. The exceptions were the Bothnian Sea and Bothnian Bay, where the correlation dropped significantly, likely related to the effect of sea ice on air–sea heat exchange. The spread of water salinity across the models is generally larger compared to water temperature, which is most obvious in the long-term time series of deepwater salinity. The inflow dynamics of saline water from the North Sea is covered well by most models, but the magnitude, as inferred from salinity, differs as much as the simulated mean salinity of deepwater. Marine heat waves (MHWs), coastal upwelling, and stratification were also assessed. In all models, MHWs were more frequent in shallow areas and in regions with seasonal ice cover. An increase in the frequency (regionally varying between ∼50 % and 250 %) and duration (50 %–150 %) of MHWs during the last 3 decades in all models was found as well. The uncertainties were highest in the Bothnian Bay, likely due to the different trends in sea ice presence. All but one of the analyzed models overestimated upwelling frequencies along the Swedish coast, the Gulf of Finland, and around Gotland, while they underestimated upwelling in the Gulf of Riga. The onset and seasonal cycle of thermal stratification likewise differed among the models. Compared to observation-based estimates, in all models the thermocline in early spring was too deep, whereas a good match was obtained in June when the thermocline intensifies.
    Type of Medium: Online Resource
    ISSN: 1991-9603
    Language: English
    Publisher: Copernicus GmbH
    Publication Date: 2022
    detail.hit.zdb_id: 2456725-5
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  • 7
    In: Geoscientific Model Development, Copernicus GmbH, Vol. 15, No. 7 ( 2022-04-08), p. 2973-3020
    Abstract: Abstract. The Earth system model EC-Earth3 for contributions to CMIP6 is documented here, with its flexible coupling framework, major model configurations, a methodology for ensuring the simulations are comparable across different high-performance computing (HPC) systems, and with the physical performance of base configurations over the historical period. The variety of possible configurations and sub-models reflects the broad interests in the EC-Earth community. EC-Earth3 key performance metrics demonstrate physical behavior and biases well within the frame known from recent CMIP models. With improved physical and dynamic features, new Earth system model (ESM) components, community tools, and largely improved physical performance compared to the CMIP5 version, EC-Earth3 represents a clear step forward for the only European community ESM. We demonstrate here that EC-Earth3 is suited for a range of tasks in CMIP6 and beyond.
    Type of Medium: Online Resource
    ISSN: 1991-9603
    Language: English
    Publisher: Copernicus GmbH
    Publication Date: 2022
    detail.hit.zdb_id: 2456725-5
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  • 8
    In: Journal of Advances in Modeling Earth Systems, American Geophysical Union (AGU), Vol. 7, No. 1 ( 2015-03), p. 268-304
    Abstract: The regionally coupled climate model is validated for the North Atlantic Ocean circulation and biogeochemistry in the northern North Atlantic Atmospheric circulation and river runoff in Europe
    Type of Medium: Online Resource
    ISSN: 1942-2466 , 1942-2466
    URL: Issue
    Language: English
    Publisher: American Geophysical Union (AGU)
    Publication Date: 2015
    detail.hit.zdb_id: 2462132-8
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  • 9
    In: Intensive Care Medicine Experimental, Springer Science and Business Media LLC, Vol. 1, No. 1 ( 2013-12)
    Abstract: In un-resuscitated rodent models of septic shock, the peroxisome proliferator-activated receptor-β/δ (PPAR-β/δ) agonist GW0742 improved visceral organ function. Therefore, we tested the hypothesis whether GW0742 would attenuate kidney injury during long-term, resuscitated, porcine polymicrobial septic shock. Methods Six, 12, and 18 h after the induction of fecal peritonitis by inoculation of autologous feces, anesthetized, mechanically ventilated, and instrumented male pigs with pre-existing atherosclerosis resulting from familial hypercholesteremia and atherogenic diet randomly received either vehicle (dimethyl sulfoxide, n = 12) or GW0742 ( n = 10). Resuscitation comprised hydroxyethyl starch and norepinephrine infusion titrated to maintain mean arterial pressure at baseline values. Results Despite aggressive fluid resuscitation, fecal peritonitis was associated with arterial hypotension requiring norepinephrine infusion, ultimately resulting in progressive lactic acidosis and acute kidney injury. GW0742 did not beneficially affect any parameter of systemic and regional hemodynamics, gas exchange, metabolism, or organ function. The parameters of inflammation, oxidative and nitrosative stress, and organ injury (post-mortem analysis for histomorphology and markers of apoptosis) were not influenced either. Immunohistochemistry of pre-shock kidney biopsies from a previous study in this swine strain showed markedly lower PPAR-β/δ receptor expression than in healthy animals. Conclusions In swine with pre-existing atherosclerosis, the PPAR-β/δ agonist GW0742 failed to attenuate septic shock-induced circulatory failure and kidney dysfunction, most likely due to reduced receptor expression coinciding with cardiovascular and metabolic co-morbidity.
    Type of Medium: Online Resource
    ISSN: 2197-425X
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2013
    detail.hit.zdb_id: 2740385-3
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  • 10
    Online Resource
    Online Resource
    Copernicus GmbH ; 2022
    In:  Earth System Dynamics Vol. 13, No. 1 ( 2022-03-30), p. 613-631
    In: Earth System Dynamics, Copernicus GmbH, Vol. 13, No. 1 ( 2022-03-30), p. 613-631
    Abstract: Abstract. Atmospheric rivers (ARs) are important drivers of hazardous precipitation levels and are often associated with intense floods. So far, the response of ARs to climate change in Europe has been investigated using global climate models within the CMIP5 framework. However, the spatial resolution of those models (1–3∘) is too coarse for an adequate assessment of local to regional precipitation patterns. Using a regional climate model with 0.22∘ resolution, we downscaled an ensemble consisting of 1 ERA-Interim (ERAI) reanalysis data hindcast simulation, 9 global historical, and 24 climate scenario simulations following greenhouse gas emission scenarios RCP2.6, RCP4.5, and RCP8.5. The performance of the climate model to simulate AR frequencies and AR-induced precipitation was tested against ERAI. Overall, we find a good agreement between the downscaled CMIP5 historical simulations and ERAI. However, the downscaled simulations better represented small-scale spatial characteristics. This was most evident over the terrain of the Iberian Peninsula, where the AR-induced precipitation pattern clearly reflected prominent east–west topographical elements, resulting in zonal bands of high and low AR impact. Over central Europe, the models simulated a smaller propagation distance of ARs toward eastern Europe than obtained using the ERAI data. Our models showed that ARs in a future warmer climate will be more frequent and more intense, especially in the higher-emission scenarios (RCP4.5, RCP8.5). However, assuming low emissions (RCP2.6), the related changes can be mostly mitigated. According to the high-emission scenario RCP8.5, AR-induced precipitation will increase by 20 %–40 % in western central Europe, whereas mean precipitation rates increase by a maximum of only 12 %. Over the Iberian Peninsula, AR-induced precipitation will slightly decrease (∼6 %) but the decrease in the mean rate will be larger (∼15 %). These changes will lead to an overall increased fractional contribution of ARs to heavy precipitation, with the greatest impact over the Iberian Peninsula (15 %–30 %) and western France (∼15 %). Likewise, the fractional share of yearly maximum precipitation attributable to ARs will increase over the Iberian Peninsula, the UK, and western France. Over Norway, average AR precipitation rates will decline by −5 % to −30 %, most likely due to dynamic changes, with ARs originating from latitudes 〉 60∘ N decreasing by up to 20 % and those originating south of 45∘ N increasing. This suggests that ARs over Norway will follow longer routes over the continent, such that additional moisture uptake will be impeded. By contrast, ARs from 〉60∘ N will take up moisture from the North Atlantic before making landfall over Norway. The found changes in the local AR pathway are probably driven by larger-scale circulation changes such as a change in dominating weather regimes and/or changes in the winter storm track over the North Atlantic.
    Type of Medium: Online Resource
    ISSN: 2190-4987
    Language: English
    Publisher: Copernicus GmbH
    Publication Date: 2022
    detail.hit.zdb_id: 2578793-7
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