ISSN:
1573-7330
Keywords:
mouse in vitro fertilization (IVF)
;
superovulation
;
preimplantation development
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract The early embryonic development of in vitro fertilized oocytes was assessed following superovulation in F1 hybrid C57BL/6×CBA/Ca mice. Decreasing the time interval between the administration of constant doses of pregnant mare's serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG) resulted in decreases in the frequency of development to the blastocyst stage but had no significant effect on development to the two-cell stage. Preincubation of postovulatory oocytes in vitro prior to insemination did not compensate for the reduced preovulatory development in vivo but resulted in decreases in the frequency of development to the blastocyst stage. The results indicate that inadequate preovulatory development of superovulated mouse oocytes can adversely affect the preimplantation development of in vitro fertilized embryos in the absence of a visible inhibitory effect on development to the two-cell stage and also that preincubation of postovulatory oocytes in vitro prior to fertilization reduces subsequent developmental capacity.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01555450
