ISSN:
1432-0983
Schlagwort(e):
Yeast
;
Heterologous gene expression
;
Sulfolobus
;
Hyperthermophile phosphoglycerate kinase
;
Archaea
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Abstract The gene encoding phosphoglycerate kinase (PGK) from the ArchaeonSulfolobus solfataricus, an organism growing optimally at 87°C, was inserted into a yeast expression vector under the control of the galactose-inducibleGAL1 yeast promoter. This vector was then transformed into apgk::TRP1 yeast mutant, a strain inhibited for growth on galactose or glucose due to its lack of PGK enzyme. Slow-growing transformants were obtained on galactose plates at 37°C, but not 28°C. These transformants contained low levels of transcripts of the heterologous gene and low amounts of thermostable PGK activity. Weak expression of the hyperthermophile gene in yeast a mesophile, therefore enabled complementation of the yeastpgk defect at 37°C but not at 28°C.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF02426966