Abstract
The gene encoding phosphoglycerate kinase (PGK) from the ArchaeonSulfolobus solfataricus, an organism growing optimally at 87°C, was inserted into a yeast expression vector under the control of the galactose-inducibleGAL1 yeast promoter. This vector was then transformed into apgk::TRP1 yeast mutant, a strain inhibited for growth on galactose or glucose due to its lack of PGK enzyme. Slow-growing transformants were obtained on galactose plates at 37°C, but not 28°C. These transformants contained low levels of transcripts of the heterologous gene and low amounts of thermostable PGK activity. Weak expression of the hyperthermophile gene in yeast a mesophile, therefore enabled complementation of the yeastpgk defect at 37°C but not at 28°C.
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Communicated by F.K. Zimmermann
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Piper, P.W., Emson, C., Jones, C.E. et al. Complementation of apgk deletion mutation inSaccharomyces cerevisiae with expression of the phosphoglycerate-kinase gene from the hyperthermophilic ArchaeonSulfolobus solfataricus . Curr Genet 29, 594–596 (1996). https://doi.org/10.1007/BF02426966
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DOI: https://doi.org/10.1007/BF02426966