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  • 1
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 374 (1995), S. 262-266 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Synaptic currents were monitored on single innervated myocytes by whole-cell voltage-clamp recordings in 1-day-old nerve-muscle cultures prepared from Xenopus embryos16. We first examined the effects of the NO donor, S-nitroso-7V-acetyl-penicilamine (SNAP)17, on spontaneous synaptic currents ...
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Field excitatory postsynaptic potentials (EPSPs) were elicited in hippocampal slices of different ages by stimulating two independent Schaffer-commissural afferents converging on the same CA1 neurons12. Similar to previous observations8"10, the magnitude and longevity of theta-burst stimulation ...
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 179 (1999), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A mini-Tn5 transposon derivative, mini-Tn5gfp-km, has been constructed which contained a promoter-less artificial operon consisting of two open reading frames, green fluorescent protein (GFP) and neomycin phosphotransferase II (NptII). When this transposon was used to mutagenize Agrobacterium tumefaciens, all the mutants selected in the presence of kanamycin exhibited GFP expression, which could be conveniently monitored by a fluorometer. The transposon appeared to be bifunctional and could provide both selection and reporter functions. Even the mutants showing minimal levels of GFP expression were still resistant to kanamycin. This suggests that this transposon can be used to select for insertions downstream of both weak and strong promoters, as long as the insertions themselves are non-lethal. This system was used to identify A. tumefaciens genes that were upregulated in response to acidic pH. Screening only 20 colonies led to identification of two promoters that were specifically induced by low pH and one promoter that was specifically induced by acetosyringone in a minimal medium of pH 5.5.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 363 (1993), S. 76-79 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The effect of CGRP on spontaneous synaptic currents (SSCs) was examined in 1-day-old Xenopus nerve-muscle cultures5'6. Amplitude and decay time, but not rise time, exhibited sig nificant increases after CGRP treatment (Fig. 1; Table la). The effects of CGRP are readily seen in the histograms of SSC ...
    Type of Medium: Electronic Resource
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  • 5
    Publication Date: 2016-03-26
    Description: Article Gene expression noise affects cell fitness and development. Here, Yan et al . show that co-regulated divergent gene pairs (DGPs) suppress uncorrelated gene expression noise due to more synchronized transcription firing, and differentially regulated DGPs enhance gene expression noise due to transcription leakage. Nature Communications doi: 10.1038/ncomms11099 Authors: Chao Yan, Shuyang Wu, Christopher Pocetti, Lu Bai
    Electronic ISSN: 2041-1723
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General , Physics
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  • 6
    Publication Date: 2014-08-06
    Description: Background: Age-related physiological, biochemical and functional changes in mammalian skeletal muscle have been shown to begin at the mid-point of the lifespan. However, the underlying changes in DNA methylation that occur during this turning point of the muscle aging process have not been clarified. To explore age-related genomic methylation changes in skeletal muscle, we employed young (0.5 years old) and middle-aged (7 years old) pigs as models to survey genome-wide DNA methylation in the longissimus dorsi muscle using a methylated DNA immunoprecipitation sequencing approach. Results: We observed a tendency toward a global loss of DNA methylation in the gene-body region of the skeletal muscle of the middle-aged pigs compared with the young group. We determined the genome-wide gene expression pattern in the longissimus dorsi muscle using microarray analysis and performed a correlation analysis using DMR (differentially methylated region)-mRNA pairs, and we found a significant negative correlation between the changes in methylation levels within gene bodies and gene expression. Furthermore, we identified numerous genes that show age-related methylation changes that are potentially involved in the aging process. The methylation status of these genes was confirmed using bisulfite sequencing PCR. The genes that exhibited a hypomethylated gene body in middle-aged pigs were over-represented in various proteolysis and protein catabolic processes, suggesting an important role for these genes in age-related muscle atrophy. In addition, genes associated with tumorigenesis exhibited aged-related differences in methylation and expression levels, suggesting an increased risk of disease associated with increased age. Conclusions: This study provides a comprehensive analysis of genome-wide DNA methylation patterns in aging pig skeletal muscle. Our findings will serve as a valuable resource in aging studies, promoting the pig as a model organism for human aging research and accelerating the development of comparative animal models in aging research.
    Electronic ISSN: 1471-2164
    Topics: Biology
    Published by BioMed Central
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  • 7
    Publication Date: 2012-07-05
    Description: Mesenchymal stem cells (MSCs) have potential applications in regenerative medicine and tissue engineering as well as being potential carriers for tumour therapy. However, the safety of using MSCs in tumours is unknown. Herein, we analyse malignant transformation of MSCs in the tumour microenvironment. Rat bone marrow MSCs were cultured with malignant rat glioma C6 cells without direct cell–cell contact. After 7 days, the cells were assessed for transformation using flow cytometry, real-time quantitative PCR, immunofluorescence and chromosomal analysis. In addition, wild-type (WT) p53, mutant p53 and mdm2 was determined using Western blotting. Almost all MSCs became phenotypically malignant cells, with significantly decreased WT p53 expression and increased expression of mutant p53 and mdm2, along with an aneuploid karyotype. To evaluate tumorigenesis in vivo , the MSCs indirect co-cultured with C6 cells for 7 days were transplanted subcutaneously into immuno-deficient mice. The cells developed into a large tumour at the injection site within 8 weeks, with systemic symptoms including cachexia and scoliosis. Pathological and cytological analysis revealed poorly differentiated pleomorphic cells with a dense vascular network and aggressive invasion into the adjacent muscle. These data demonstrate that MSCs became malignant cancer cells when exposed to the tumour microenvironment and suggest that factors released from the cancer cells have a critical role in the malignant transformation of MSCs. Copyright © 2012 John Wiley & Sons, Ltd.
    Print ISSN: 0263-6484
    Electronic ISSN: 1099-0844
    Topics: Biology , Medicine
    Published by Wiley-Blackwell
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  • 8
    Publication Date: 2016-11-27
    Description: Recently genome-wide association studies identified that NCAN rs2228603 polymorphism was associated with non-alcoholic fatty liver disease (NAFLD) mainly in subjects of European ancestry. While no research have b...
    Electronic ISSN: 1476-511X
    Topics: Biology
    Published by BioMed Central
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  • 9
    Publication Date: 2017-07-22
    Description: VOLUME 288 (2013) PAGES 24247–24263This article has been withdrawn by the authors. After reviewing the data, the authors found that the first author of the paper made inappropriate modifications to the background of the following figures: Figs. 2C, 2D, 3B, 3E, 4A, 4E, 5E, 6D, 8A, 8C, and supplemental Figs. S1A, S2B, and S5B. The first author admits to making the modifications. The modifications include covering cells not expressing RFP-GFP-LC3, GFP-LC3, or RFP-Lamp, incomplete cells on the edge of the images, or contaminant staining dots outside of the cells in images (Figs. 2C, 2D, 3B, 3E, 4A, 4E, 6D, 8A, 8C, and supplemental Figs. S1A and S2B). Additionally, other modifications include covering dirty dots above the bands in the Western blots (Fig. 5E and supplemental Fig. S5B) and inadvertently inserting a box in LC3 blot of Fig. 4E. Although these modifications did not change the results or interpretations of this work, these figures were not prepared according to the publication policy of the journal. Therefore, the authors wish to withdraw this article and sincerely apologize for these mistakes.
    Print ISSN: 0021-9258
    Electronic ISSN: 1083-351X
    Topics: Biology , Chemistry and Pharmacology
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