GLORIA

GEOMAR Library Ocean Research Information Access

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    facet.materialart.
    Unknown
    AssessingSymbiodiniumdiversity in scleractinian corals via next-generation sequencing-based genotyping of the ITS2 rDNA region (2014)
    Wiley
    In:  Molecular Ecology, 23 (17). pp. 4418-4433.
    Details
    Publication Date: 2019-09-23
    Description: The persistence of coral reef ecosystems relies on the symbiotic relationship between scleractinian corals and intracellular, photosynthetic dinoflagellates in the genus Symbiodinium. Genetic evidence indicates that these symbionts are biologically diverse and exhibit discrete patterns of environmental and host distribution. This makes the assessment of Symbiodinium diversity critical to understanding the symbiosis ecology of corals. Here, we applied pyrosequencing to the elucidation of Symbiodinium diversity via analysis of the internal transcribed spacer 2 (ITS2) region, a multicopy genetic marker commonly used to analyse Symbiodinium diversity. Replicated data generated from isoclonal Symbiodinium cultures showed that all genomes contained numerous, yet mostly rare, ITS2 sequence variants. Pyrosequencing data were consistent with more traditional denaturing gradient gel electrophoresis (DGGE) approaches to the screening of ITS2 PCR amplifications, where the most common sequences appeared as the most intense bands. Further, we developed an operational taxonomic unit (OTU)-based pipeline for Symbiodinium ITS2 diversity typing to provisionally resolve ecologically discrete entities from intragenomic variation. A genetic distance cut-off of 0.03 collapsed intragenomic ITS2 variants of isoclonal cultures into single OTUs. When applied to the analysis of field-collected coral samples, our analyses confirm that much of the commonly observed Symbiodinium ITS2 diversity can be attributed to intragenomic variation. We conclude that by analysing Symbiodinium populations in an OTU-based framework, we can improve objectivity, comparability and simplicity when assessing ITS2 diversity in field-based studies.
    Type: Article , PeerReviewed
    Format: text
    DOI: 10.1111/mec.12869
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    OceanRep: Article in a Scientific Journal - peer-reviewed
  • 2
    facet.materialart.
    Unknown
    Microbiome structure of the fungid coral Ctenactis echinataaligns with environmental differences (2015)
    Wiley
    In:  Molecular Ecology, 24 (13). pp. 3501-3511.
    Details
    Publication Date: 2017-12-19
    Description: The significance of bacteria for eukaryotic functioning is increasingly recognized. Coral reef ecosystems critically rely on the relationship between coral hosts and their intracellular photosynthetic dinoflagellates, but the role of the associated bacteria remains largely theoretical. Here, we set out to relate coral-associated bacterial communities of the fungid host species Ctenactis echinata to environmental settings (geographic location, substrate cover, summer/winter, nutrient and suspended matter concentrations) and coral host abundance. We show that bacterial diversity of C.echinata aligns with ecological differences between sites and that coral colonies sampled at the species' preferred habitats are primarily structured by one bacterial taxon (genus Endozoicomonas) representing more than 60% of all bacteria. In contrast, host microbiomes from lower populated coral habitats are less structured and more diverse. Our study demonstrates that the content and structure of the coral microbiome aligns with environmental differences and denotes habitat adequacy. Availability of a range of coral host habitats might be important for the conservation of distinct microbiome structures and diversity.
    Type: Article , PeerReviewed
    Format: text
    DOI: 10.1111/mec.13251
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    OceanRep: Article in a Scientific Journal - peer-reviewed
  • 3
    facet.materialart.
    Unknown
    PCR and DNA Sequencing (2014)
    Wiley
    In:  In: Biofouling Methods. , ed. by Dobretsov, S., Williams, D. N. and Thomason, J. Wiley, New York, USA, pp. 93-113. ISBN 978-0-470-65985-4
    Details
    Publication Date: 2014-11-06
    Type: Book chapter , NonPeerReviewed
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    OceanRep: Book chapter
  • 4
    facet.materialart.
    Unknown
    Revealing microbial functional activities in the Red Sea sponge Stylissa carteriby metatranscriptomics (2014)
    Wiley
    In:  Environmental Microbiology, 16 (12). pp. 3683-3698.
    Details
    Publication Date: 2017-03-30
    Description: Sponges are important components of marine benthic environments and are associated with microbial symbionts that carry out ecologically relevant functions. Stylissa carteri is an abundant, low-microbial abundance species in the Red Sea. We aimed to achieve the functional and taxonomic characterization of the most actively expressed prokaryotic genes in S. carteri. Prokaryotic mRNA was enriched from sponge total RNA, sequenced using Illumina HiSeq technology and annotated using the metagenomics Rapid Annotation using Subsystem Technology (MG-RAST) pipeline. We detected high expression of archaeal ammonia oxidation and photosynthetic carbon fixation by members of the genus Synechococcus. Functions related to stress response and membrane transporters were among the most highly expressed by S. carteri symbionts. Unexpectedly, gene functions related to methylotrophy were highly expressed by gammaproteobacterial symbionts. The presence of seawater-derived microbes is indicated by the phylogenetic proximity of organic carbon transporters to orthologues of members from the SAR11 clade. In summary, we revealed the most expressed functions of the S. carteri-associated microbial community and linked them to the dominant taxonomic members of the microbiome. This work demonstrates the applicability of metatranscriptomics to explore poorly characterized symbiotic consortia and expands our knowledge of the ecologically relevant functions carried out by coral reef sponge symbionts.
    Type: Article , PeerReviewed
    Format: text
    DOI: 10.1111/1462-2920.12533
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    OceanRep: Article in a Scientific Journal - peer-reviewed
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...