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  • 1995-1999  (2)
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  • 1
    Electronic Resource
    Electronic Resource
    Expression in Escherichia coli and properties of the carotene ketolase from Haematococcus pluvialis (1996)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 140 (1996), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract High level expression of the functional β-carotene ketolase gene bkt from Haematococcus pluvialis occurred in Escherichia coli transformants producing β-carotene or zeaxanthin as a result of the presence of additional carotenoid genes from Erwinia uredovora. Requirement of molecular oxygen for the insertion of the keto group was demonstrated. The final product of this two-step ketolase reaction from β-carotene is canthaxanthin (4,4′-diketo-β-carotene) with the 4-monoketo derivative echinenone as an intermediate. A reaction sequence for the formation of astaxanthin from β-carotene was established based on kinetic data on astaxanthin formation in E. coli transformants carrying the hydroxylase gene crtZ from Erwinia along with bkt. We conclude that the carotenoids zeaxanthin and adonixanthin which accumulate in addition to astaxanthin in this transformant are products of side reactions rather than direct precursors of astaxanthin. The possible mechanisms for the formation of the keto derivatives are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1996.tb08343.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    A higher-plant type ζ-carotene desaturase in the cyanobacterium Synechocystis PCC6803 (1998)
    Springer
    Plant molecular biology 36 (1998), S. 725-732 
    Details
    ISSN: 1573-5028
    Keywords: ζ-Carotene desaturase gene ; J852 ; lycopene ; neurosporene ; phylogeny ; Synechocystis PCC6803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The genomic DNA sequence of Synechocystis was analysed for putative ζ-carotene desaturase genes. Two promising candidates slr0940 and slr0033 were found with similarities to the structurally different ζ-carotene desaturase genes from higher plants and Anabaena, respectively. Only the expression product of the analogue to the plant gene, slr0940, was able to mediate the 2-step desaturation of ζ-carotene via neurosporene to lycopene after complementation of this pathway in Escherichia coli. When enzyme reactions were carried out with this protein, activity was obtained with either ζ-carotene or neuroporene as substrates. The in vitro reaction was inhibited by the pyrimidine derivative J852 which is effective as experimental herbicide in plants. The occurrence of two different types of ζ-carotene desaturases among cyanobacteria and the phylogenetic consequences on chloroplast evolution are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005997405431
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    Paper (German National Licenses)
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