ISSN:
1573-6776
Keywords:
ammonium content
;
bialaphos-resistance gene
;
photostable selection system
;
Synechococcus sp. strain PCC7942
;
transformation frequency
Source:
Springer Online Journal Archives 1860-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract A novel cyanobacterial vector, pTT201, containing the bar gene encoding resistance to herbicides, bialaphos and phosphinothricin, was constructed. In Synechococcus sp. strain PCC7942-SPc, the bar gene was successfully expressed. Plasmid pTT201 increased a minimum inhibitory concentration for bialaphos 16-fold over Synechococcus sp. strain PCC7942-SPc without pTT201. The combination of the bialaphos as a selective agent and the transformation by bar gene serves as a photostable selection system for Synechococcus.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1005595211662
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