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  • 2020-2023  (5)
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  • 1
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    In:  EPIC3Biologisches Kolloquium, Universität Rostock, Institut für Biowissenschaften, 2021-11-11-2021-11-11
    Publikationsdatum: 2022-02-09
    Repository-Name: EPIC Alfred Wegener Institut
    Materialart: Conference , notRev
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 2
    Publikationsdatum: 2022-10-26
    Beschreibung: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Cresswell, T., Metian, M., Fisher, N. S., Charmasson, S., Hansman, R. L., Bam, W., Bock, C., & Swarzenski, P. W. Exploring new frontiers in marine radioisotope tracing - adapting to new opportunities and challenges. Frontiers in Marine Science, 7, (2020): 406, doi:10.3389/fmars.2020.00406.
    Beschreibung: Radioisotopes have been used in earth and environmental sciences for over 150 years and provide unique tools to study environmental processes in great detail from a cellular level through to an oceanic basin scale. These nuclear techniques have been employed to understand coastal and marine ecosystems via laboratory and field studies in terms of how aquatic organisms respond to environmental stressors, including temperature, pH, nutrients, metals, organic anthropogenic contaminants, and biological toxins. Global marine issues, such as ocean warming, deoxygenation, plastic pollution, ocean acidification, increased duration, and intensity of toxic harmful algal blooms (HABs), and coastal contamination are all impacting marine environments, thereby imposing various environmental and economic risks. Being able to reliably assess the condition of coastal and marine ecosystems, and how they may respond to future disturbances, can provide vital information for society in the sustainable management of their marine environments. This paper summarizes the historical use of radiotracers in these systems, describes how existing techniques of radioecological tracing can be developed for specific current environmental issues and provides information on emerging issues that would benefit from current and new radiotracer methods. Current challenges with using radioecological tracers and opportunities are highlighted, as well as opportunities to maximize the application of these methods to greatly increase the ability of environmental managers to conduct evidence-based management of coastal and marine ecosystems.
    Beschreibung: The IAEA is grateful for the support provided to its Environment Laboratories by the Government of the Principality of Monaco. This contribution was made within the framework of the IAEA CRP on “Applied radioecological tracers to assess coastal and marine ecosystem health” (K41019).
    Schlagwort(e): Radionuclides ; Radiotracers ; Radioecology ; Ecosystem condition ; Marine ; Coastal
    Repository-Name: Woods Hole Open Access Server
    Materialart: Article
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 3
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    In:  EPIC3114th Annual Meeting of the German Zoological Society, University Bonn, 2022-09-13-2022-09-16
    Publikationsdatum: 2022-10-17
    Beschreibung: Modern non-destructive imaging techniques offer distinct advantages for particular anatomical and morphological questions in zoology. The possibility to capture multi- slice images and create 3D representations of complex and fragile morphological structures that can be digitized and animated in combination with image processing techniques are just some of the reasons why these techniques are increasingly being used. Using the Antarctic silverfish Pleuragramma antarctica as an example, we show in this presentation how high-field magnetic resonance imaging (MRI) can be used as a tool to address ecological questions in preserved animal samples. P. antarctica is a pelagic key species in the food web of high-Antarctic waters and is considered highly vulnerable to changes in its environment. P. antarctica is extremely delicate and capturing and keeping individuals of this species alive for in vivo imaging studies is hardly possible. Due to the lack of a swim bladder, neutral buoyancy is mainly attained by large amounts of lipids which are stored in lipid sacks. However, the functional role of lipids in P. antarctica is not yet fully understood, i.e. it is not clear whether the function of lipids is limited to buoyancy or they serve as energy deposits as well. If the lipids are used as energy storage, differences in the nutritional state should be reflected in the amount of body lipid content. Several examples of imaging applications for addressing ecological questions in P. antarctica will be presented. This will include high-resolution morphological 2D- and 3D-MR images from P. antarctica to determine body composition and the overall fat and muscle distribution of individual preserved fishes. We calculated the individual lipid and water content from these images, which allowed us to estimate the percentage of overall lipid content. The percentage of lipid content correlated well with literature data obtained from standard, destructive lipid measurement techniques. 3D image reconstructions were used for non-destructive analysis of stomach volume and comparing lipid deposits in different individuals. We show that functional morphological MRI is a useful tool in marine ecological studies, even in preserved samples and provides a suitable alternative to some classical, destructive methods, in particular for studies of fragile and delicate structure.
    Repository-Name: EPIC Alfred Wegener Institut
    Materialart: Conference , NonPeerReviewed
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 4
    Publikationsdatum: 2022-10-17
    Beschreibung: Oxygen fluctuations are common in marine waters, and hypoxia– reoxygenation (H–R) stress can negatively affect mitochondrial metabolism. The long-lived ocean quahog, Arctica islandica, is known for its hypoxia tolerance associated with metabolic rate depression, yet the mechanisms that sustain mitochondrial function during oxygen fluctuations are not well understood. We used top-down metabolic control analysis (MCA) to determine aerobic capacity and control over oxygen flux in the mitochondria of quahogs exposed to short-term hypoxia (24 h 〈0.01% O2) and subsequent reoxygenation (1.5 h 21% O2) compared with normoxic control animals (21% O2). We demonstrated that flux capacity of the substrate oxidation and proton leak subsystems were not affected by hypoxia, while the capacity of the phosphorylation subsystem was enhanced during hypoxia associated with a depolarization of the mitochondrial membrane. Reoxygenation decreased the oxygen flux capacity of all three mitochondrial subsystems. Control over oxidative phosphorylation (OXPHOS) respiration was mostly exerted by substrate oxidation regardless of H–R stress, whereas control by the proton leak subsystem of LEAK respiration increased during hypoxia and returned to normoxic levels during reoxygenation. During hypoxia, reactive oxygen species (ROS) efflux was elevated in the LEAK state, whereas it was suppressed in the OXPHOS state. Mitochondrial ROS efflux returned to normoxic control levels during reoxygenation. Thus, mitochondria of A. islandica appear robust to hypoxia by maintaining stable substrate oxidation and upregulating phosphorylation capacity, but remain sensitive to reoxygenation. This mitochondrial phenotype might reflect adaptation of A. islandica to environments with unpredictable oxygen fluctuations and its behavioural preference for low oxygen levels.
    Repository-Name: EPIC Alfred Wegener Institut
    Materialart: Article , isiRev
    Format: application/pdf
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 5
    Publikationsdatum: 2022-08-19
    Beschreibung: Low-salinity stress can severely affect the fitness of marine organisms. As desalination has been predicted for many coastal areas with ongoing climate change, it is crucial to gain more insight in mechanisms that constrain salinity acclimation ability. Low-salinity induced depletion of the organic osmolyte pool has been suggested to set a critical boundary in osmoconforming marine invertebrates. Whether inorganic ions also play a persistent role during low-salinity acclimation processes is currently inconclusive. We investigated the salinity tolerance of six marine invertebrate species following a four-week acclimation period around their low-salinity tolerance threshold. To obtain complete osmolyte budgets, we quantified organic and inorganic osmolytes and determined fitness proxies. Our experiments corroborated the importance of the organic osmolyte pool during low-salinity acclimation. Methylamines constituted a large portion of the organic osmolyte pool in molluscs, whereas echinoderms exclusively utilized free amino acids. Inorganic osmolytes were involved in long-term cellular osmoregulation in most species, thus are not just modulated with acute salinity stress. The organic osmolyte pool was not depleted at low salinities, whilst fitness was severely impacted. Instead, organic and inorganic osmolytes often stabilized at low-salinity. These findings suggest that low- salinity acclimation capacity cannot be simply predicted from organic osmolyte pool size. Rather, multiple parameters (i.e. osmolyte pools, net growth, water content and survival) are necessary to establish critical salinity ranges. However, a quantitative knowledge of cellular osmolyte systems is key to understand the evolution of euryhalinity and to characterize targets of selection during rapid adaptation to ongoing desalination.
    Repository-Name: EPIC Alfred Wegener Institut
    Materialart: Article , NonPeerReviewed
    Format: application/pdf
    Standort Signatur Einschränkungen Verfügbarkeit
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