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  • 1
    Electronic Resource
    Electronic Resource
    Optimum infection conditions for recombinant protein production in insect cell (Bm5) suspension culture (1994)
    s.l. : American Chemical Society
    Biotechnology progress 10 (1994), S. 636-643 
    Details
    ISSN: 1520-6033
    Source: ACS Legacy Archives
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bp00030a008
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Development of a versatile computer integrated control system for bioprocess controls (1998)
    Springer
    Cytotechnology 26 (1998), S. 227-236 
    Details
    ISSN: 1573-0778
    Keywords: bioreactor ; computer control ; data acquisition ; glucose control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A general approach is described for the implementation of a networked multi-unit computer integrated control system. The use of data acquisition hardware and graphical programming tools alleviates tedious programming and maintains potency and flexibility. One application of the control system, the control of a mammalian cell perfusion culture based on a key nutrient glucose concentration, was demonstrated. The control system offers customized user interface for all process control parameters and allows the flexibility for continued improvement and implementation of new tailored functions. The temperature, pH, dissolved oxygen and glucose level were accurately controlled.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007948313304
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Long-term stable production of monocyte-colony inhibition factor (M-CIF) from CHO microcarrier perfusion cultures (1998)
    Springer
    Cytotechnology 26 (1998), S. 131-138 
    Details
    ISSN: 1573-0778
    Keywords: CHO ; microcarrier ; M-CIF ; perfusion ; spin filter ; stable production
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Monocyte-colony inhibition factor (M-CIF) was produced in microcarrier perfusion cultures from engineered Chinese hamster ovary (CHO) cells. Three and fifteen liter microcarrier perfusion bioreactors equipped with internal spin filters were operated for over two months. Approximately 60 L and 300 L of culture filtrate were harvested from the 3L and 15L microcarrier perfusion bioreactors respectively. During the perfusion operation, cell density reached 2–6 × 106 cells/ml. Importantly, stable expression of M-CIF from the CHO cells under non-selection condition was maintained at a level of 4–10 mg/L. Specific productivity was maintained at 1.8–3.4 mg/billion cells/day. The ability of the recombinant CHO cells to migrate from microcarrier to microcarrier under our proprietary HGS-CHO-3 medium greatly facilitated microcarrier culture scale-up and microcarrier replenishment. Future directions for microcarrier perfusion system scale-up and process development are highlighted.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007997412002
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    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    A two-stage bioreactor system for the production of recombinant proteins using a genetically engineered baculovirus/insect cell system (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 42 (1993), S. 357-366 
    Details
    ISSN: 0006-3592
    Keywords: bioreactor ; insect cell culture ; high-density cell culture ; recombinant baculovirus ; chloramphenicol acetyltransferase ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A two-stage bioreactor scheme was developed for the large-scale production of recombinant proteins using a genetically engineered baculovirus/insect cell system. The first bioreactor was employed for cell growth and the second for cell infection. Silkworm Bm5 cells were infected with a recombinant baculovirus, BmNPV/P5.cat, containing a bacterial chloramphenicol acetyltransferase (CAT) gene under the control of the polyhedrin gene promoter of Bombyx mori nuclear polyhedrosis virus (BmNPV). This recombinant baculovirus has been used as an expression vector for the production of recombinant CAT enzyme. A specific productivity of 82 to 90 μg CAT/(106 cells) was obtained using the BmNPV/Bm5 expression system, a yield similar to that achieved using the AcNPV/Sf expression system. Repeated infection of high-density cell cultures did not reduce the specific productivity of the CAT enzyme. Most importantly, the problems associated with the infection of high-density cell cultures were resolved by means of controlled infection conditions and appropriate replenishment of spent culture medium following infection. The glucose uptake rate by the cells following infection was 50% higher than that by the cells before infection. Not only did the infection of high-density cell cultures result in consistent yields of 250 mg/L of CAT enzyme, but also the two-stage bioreactor system was proven to be reliable for a long-term operation beyond 600 h. © 1993 John Wiley & Sons, Inc.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260420313
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    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Investigation of reduced serum and serum-free media for the cultivation of insect cells (Bm5) and the production of baculovirus (BmNPV) (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 40 (1992), S. 1165-1172 
    Details
    ISSN: 0006-3592
    Keywords: insect cell culture ; baculovirus ; reduced serum medium ; serum-free medium ; Bm5 cells ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An experimental study has been carried out to investigate the effectivenes of several reduced serum and serum-free media for the cultivation of an ovarian cell line, Bm5, of the lepidopteran insect Bombyx mori. Bm5 cell were successfully adapted to grow in a medium containing 5% serum and a serum-free medium (EX-CELL 400). On the other hand, this cell line could not be adapted to grow in several other media suggested in the literature, including IPL-41 + 2% fetal bovine serum (FBS), SF-900, and a serum-free medium (ISFM). Furthermore, a comparative study was conducted to determine the production levels of B. mori nuclear polyhedrosis virus (BmNPV) in Bm5 cells cultured in three different medium formulations. The production levels of BmNPV in adapted Bm5 cells grown in a 5% serum-supplemented medium and a serum-free medium (EX-CELL 400) were comparable to those obtained in Bm5 cells grown 10% serum-supplemented medium. © 1992 John Wiley & Sons, Inc.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260401005
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    Paper (German National Licenses)
    Fulltext
  • 6
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    Moisture and density of Site C0023 (2020)
    PANGAEA
    Details
    Publication Date: 2023-06-14
    Keywords: 370-C0023A; Chikyu; density; Density, grain; Density, wet bulk; DEPTH, sediment/rock; DRILL; Drilling/drill rig; Exp370; Integrated Ocean Drilling Program / International Ocean Discovery Program; IODP; moisture; Nankai Trough; Porosity; Sample code/label; Sample ID; Water content, wet mass
    Type: Dataset
    Format: text/tab-separated-values, 66 data points
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    DATA PANGAEA
  • 7
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    Consolidation properties of Site C0023 (2020)
    PANGAEA
    Details
    Publication Date: 2023-06-14
    Keywords: 370-C0023A; Chikyu; Compression index; consolidation test; DEPTH, sediment/rock; DRILL; Drilling/drill rig; Exp370; Expansion index; Hydrostatic pressure; Integrated Ocean Drilling Program / International Ocean Discovery Program; IODP; Nankai Trough; Overburden pressure; preconsolidation pressure; Preconsolidation pressure; Recompression index; Sample code/label; Sample ID; Vertical effective stress
    Type: Dataset
    Format: text/tab-separated-values, 110 data points
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    DATA PANGAEA
  • 8
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    Moisture, density, and consolidation properties of IODP Site C0023 from the Shallow Nankai Trough Subduction Zone, SW Japan (2020)
    PANGAEA
    Details
    Publication Date: 2023-06-14
    Description: A total of 11 whole-round core samples from 450 to 1022 mbsf were recovered at Site C0023 during IODP Expedition 370. Moisture and density measurements and consolidation experiments were conducted in a shore-based laboratory. Moisture and density data include wet bulk density, grain density, porosity and water content, whereas consolidation experiments yield preconsolidation pressure, recompression index, compression index and expansion index.
    Keywords: density; Integrated Ocean Drilling Program / International Ocean Discovery Program; IODP; moisture
    Type: Dataset
    Format: application/zip, 2 datasets
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    DATA PANGAEA
  • 9
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    On the consolidation state of sediments from the accretionary prism offshore Vancouver Island, North Cascadia Margin (2022)
    Springer
    Details
    Publication Date: 2024-02-07
    Description: Sediment geotechnical properties are an important component of geohazard analyses and studies on submarine slope failures on continental margins. The northern Cascadia margin is a region of repeated subduction earthquakes and related geohazards including devastating tsunamis. Despite several drilling campaigns at this margin mostly for gas hydrate studies, sediment geotechnical properties are lacking to date. We therefore investigated a set of 20 half-round samples from four drill sites of Integrated Ocean Drilling Program (IODP) Expedition 311 along a margin-perpendicular transect. Samples were analysed for Atterberg properties (liquid limit, plasticity limit, plasticity index) and consolidation state (pre-consolidation stress, over-consolidation ratio, initial void ratio and compression index). Results of these parameters are in good agreement with similar studies at the southern Cascadia margin from Ocean Drilling Program (ODP) Leg 204 and are regarded robust, despite the long time-span between sample recovery in 2005 and our analyses. Individual results are interpreted at each of the drill sites and reflect the overall tectonic state of the location within the accretionary prism. An interplay of site-specific tectonic forcing, including thrust faulting, uplift, folding, and erosion appears to govern the depth-dependent consolidation state of the sediments.
    Type: Article , PeerReviewed
    Format: text
    Format: text
    Format: text
    Format: text
    Format: text
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    OceanRep: Article in a Scientific Journal - peer-reviewed
  • 10
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    Pre-seizure state identified by diffuse optical tomography (2014)
    Nature Publishing Group (NPG)
    Details
    Publication Date: 2014-01-22
    Description: In epilepsy it has been challenging to detect early changes in brain activity that occurs prior to seizure onset and to map their origin and evolution for possible intervention. Here we demonstrate using a rat model of generalized epilepsy that diffuse optical tomography (DOT) provides a unique functional neuroimaging modality for noninvasively and continuously tracking such brain activities with high spatiotemporal resolution. We detected early hemodynamic responses with heterogeneous patterns, along with intracranial electroencephalogram gamma power changes, several minutes preceding the electroencephalographic seizure onset, supporting the presence of a “pre-seizure” state. We also observed the decoupling between local hemodynamic and neural activities. We found widespread hemodynamic changes evolving from local regions of the bilateral cortex and thalamus to the entire brain, indicating that the onset of generalized seizures may originate locally rather than diffusely. Together, these findings suggest DOT represents a powerful tool for mapping early seizure onset and propagation pathways. Scientific Reports 4 doi: 10.1038/srep03798
    Electronic ISSN: 2045-2322
    Topics: Natural Sciences in General
    Published by Nature Publishing Group (NPG)
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    PAPER CURRENT
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