Note: Cover -- Half Title -- Title Page -- Copyright Page -- Preface -- Acknowledgments -- Contents -- CHAPTER 1 AN INTRODUCTION TO HUMAN EVOLUTIONARY GENETICS -- 1.1 WHAT IS HUMAN EVOLUTIONARY GENETICS? -- 1.2 INSIGHTS INTO PHENOTYPES AND DISEASES -- A shared evolutionary history underpins our understanding of biology -- Understanding evolutionary history is essential to understanding human biology today -- Understanding evolutionary history shapes our expectations about the future -- 1.3 COMPLEMENTARY RECORDS OF THE HUMAN PAST -- Understanding chronology allows comparison of evidence from different scientific capproaches -- It is important to synthesize different records of the past -- None of the different records represents an unbiased picture of the past -- 1.4 WHAT CAN WE KNOW ABOUT THE PAST? -- 1.5 THE ETHICS OF STUDYING HUMAN POPULATIONS -- SUMMARY -- REFERENCES -- CHAPTER 2 ORGANIZATION AND INHERITANCE OF THE HUMAN GENOME -- 2.1 THE BIG PICTURE: AN OVERVIEW OF THE HUMAN GENOME -- 2.2 STRUCTURE OF DNA -- 2.3 GENES, TRANSCRIPTION, AND TRANSLATION -- Genes are made up of introns and exons, and include elements to initiate and regulate transcription -- The genetic code allows nucleotide sequences to be translated into amino acid sequences -- Gene expression is highly regulated in time and space -- 2.4 NONCODING DNA -- Some DNA sequences in the genome are repeated in multiple copies -- 2.5 HUMAN CHROMOSOMES AND THE HUMAN KARYOTYPE -- The human genome is divided into 46 chromosomes -- Size, centromere position, and staining methods allow chromosomes to be distinguished -- 2.6 MITOSIS, MEIOSIS, AND THE INHERITANCE OF THE GENOME -- 2.7 RECOMBINATION-THE GREAT RESHUFFLER -- 2.8 NONRECOMBINING SEGMENTS OF THE GENOME -- The male-specific Y chromosome escapes crossing over for most of its length -- Maternally inherited mtDNA escapes from recombination. , SUMMARY -- QUESTIONS -- REFERENCES -- CHAPTER 3 HUMAN GENOME VARIATION -- 3.1 GENETIC VARIATION AND THE PHENOTYPE -- Some DNA sequence variation causes Mendelian genetic disease -- The relationship between genotype and phenotype is usually complex -- Mutations are diverse and have different rates and mechanisms -- 3.2 SINGLE NUCLEOTIDE POLYMORPHISMS (SNPS) IN THE NUCLEAR GENOME -- Base substitutions can occur through base misincorporation during DNA replication -- Base substitutions can be caused by chemical and physical mutagens -- Sophisticated DNA repair processes can fix xmuch genome damage -- The rate of base substitution can be estimated indirectly or directly -- Because of their low mutation rate, SNPs usually show identity by descent -- The CpG dinucleotide is a hotspot for mutation -- Base substitutions and indels can affect the functions of genes -- Synonymous base substitutions -- Nonsynonymous base substitutions -- Indels within genes -- Base substitutions outside ORFs -- Whole-genome resequencing provides an unbiased picture of SNP diversity -- 3.3 SEQUENCE VARIATION IN MITOCHONDRIAL DNA -- mtDNA has a high mutation rate -- The transmission of mtDNA mutations between generations is complex -- 3.4 VARIATION IN TANDEMLY REPEATED DNA SEQUENCES -- Microsatellites have short repeat units and repeat arrays, and mutate through replication slippage -- Microsatellite mutation rates and processes -- Minisatellites have longer repeat units and arrays, and mutate through recombination mechanisms -- Minisatellite diversity and mutation -- Telomeres contain specialized and functionally important repeat arrays -- Satellites are large, sometimes functionally important, repeat arrays -- 3.5 TRANSPOSABLE ELEMENT INSERTIONS -- 3.6 STRUCTURAL VARIATION IN THE GENOME -- Some genomic disorders arise from recombination between segmental duplications. , Copy-number variation is widespread in the human genome -- Cytogenetic examination of chromosomes can reveal large-scale structural variants -- 3.7 THE EFFECTS OF AGE AND SEX ON MUTATION RATE -- 3.8 THE EFFECTS OF RECOMBINATION ON GENOME VARIATION -- Genomewide haplotype structure reveals past recombination behavior -- Recombination behavior can be revealed by direct studies in pedigrees and sperm DNA -- The process of gene conversion results in nonreciprocal exchange between DNA sequences -- SUMMARY -- QUESTIONS -- REFERENCES -- CHAPTER 4 FINDING AND ASSAYING GENOME DIVERSITY -- 4.1 FIRST, FIND YOUR DNA -- 4.2 THE POLYMERASE CHAIN REACTION (PCR) -- 4.3 SANGER SEQUENCING, THE HUMAN REFERENCE SEQUENCE, AND SNP DISCOVERY -- 4.4 A QUANTUM LEAP IN VARIATION STUDIES: NEXT-GENERATION SEQUENCING -- Illumina sequencing is a widely used NGS method -- Sequencing can be targeted to regions of specificinterest or the exome -- NGS data have to be processed and interpreted -- Third-generation methods use original, unamplified DNA -- 4.5 SNP TYPING: LOW-, MEDIUM-, AND HIGH- THROUGHPUT METHODS FOR ASSAYING VARIATION -- PCR-RFLP typing is a simple low-throughput method -- Primer extension and detection by mass spectrometry is a medium-throughput method -- High throughput SNP chips simultaneously analyze more than 1 million SNPs -- Whole-genome SNP chips are based on a tag SNP design -- 4.6 DATABASES OF SEQUENCE VARIATION -- 4.7 DISCOVERING AND ASSAYING VARIATION AT MICROSATELLITES -- 4.8 DISCOVERING AND ASSAYING STRUCTURAL VARIATION ON DIFFERENT SCALES -- Discovering and assaying variation at minisatellites -- Discovering and assaying variation at well-defined indels, including Alu/LINE polymorphisms -- Discovering and assaying structural polymorphisms and copy-number variants -- 4.9 PHASING: FROM GENOTYPES TO HAPLOTYPES. , Haplotypes can be determined by physical separation -- Haplotypes can be determined by statistical methods -- Haplotypes can be determined by pedigree analysis -- 4.10 STUDYING GENETIC VARIATION IN ANCIENT SAMPLES -- DNA is degraded after death -- Contamination is a major problem -- Application of next-generation sequencing to aDNA analysis -- SUMMARY -- QUESTIONS -- REFERENCES -- CHAPTER 5 PROCESSES SHAPING DIVERSITY -- 5.1 BASIC CONCEPTS IN POPULATION GENETICS -- Why do we need evolutionary models? -- The Hardy-Weinberg equilibrium is a simple model in population genetics -- 5.2 GENERATING DIVERSITY BY MUTATION AND RECOMBINATION -- Mutation changes allele frequencies -- Mutation can be modeled in different ways -- Meiotic recombination generates new combinations of alleles -- Linkage disequilibrium is a measure of recombination at the population level -- Recombination results in either crossing over or gene conversion, and is not uniform across the genome -- 5.3 ELIMINATING DIVERSITY BY GENETIC DRIFT -- The effective population size is a key concept in population genetics -- Different parts of the genome have different effective population sizes -- Genetic drift causes the fixation and elimination of new alleles -- Variation in census population size and reproductive success influence effective population size -- Population subdivision can influence effective population size -- Mate choice can influence effective population size -- Genetic drift influences the disease heritages of isolated populations -- 5.4 THE EFFECT OF SELECTION ON DIVERSITY -- Mate choice can affect allele frequencies by sexual selection -- 5.5 MIGRATION -- There are several models of migration -- There can be sex-specific differences in migration -- 5.6 INTERPLAY AMONG THE DIFFERENT FORCES OF EVOLUTION -- There are important equilibria in population genetics. , Mutation-drift balance -- Recombination-drift balance -- Mutation-selection balance -- Does selection or drift determine the future of an allele? -- 5.7 THE NEUTRAL THEORY OF MOLECULAR EVOLUTION -- The molecular clock assumes a constant rate of mutation and can allow dating of speciation -- There are problems with the assumptions of the molecular clock -- SUMMARY -- QUESTIONS -- REFERENCES -- CHAPTER 6 MAKING INFERENCES FROM DIVERSITY -- 6.1 WHAT DATA CAN WE USE? -- 6.2 SUMMARIZING GENETIC VARIATION -- Heterozygosity is commonly used to measure genetic diversity -- Nucleotide diversity can be measured using the population mutation parameter theta (θ) -- The mismatch distribution can be used to represent genetic diversity -- 6.3 MEASURING GENETIC DISTANCE -- Genetic distances between populations can be measured using F[sub(ST)] or Nei's D statistics -- Distances between alleles can be calculated using models of mutation -- Genomewide data allow calculation of genetic distances between individuals -- Complex population structure can be analyzed statistically -- Population structure can be analyzed using genomic data -- Genetic distance and population structure can be represented using multivariate analyses -- 6.4 PHYLOGENETICS -- Phylogenetic trees have their own distinctive terminology -- There are several different ways to reconstruct phylogenies -- Trees can be constructed from matrices of genetic distances -- Trees can be generated using character-based methods -- How confident can we be of a particular phylogenetic tree? -- Networks are methods for displaying multiple equivalent trees -- 6.5 COALESCENT APPROACHES TO RECONSTRUCTING POPULATION HISTORY -- The genealogy of a DNA sequence can be described mathematically -- Neutral mutations can be modeled on the gene genealogy using Poisson statistics. , Coalescent analysis can be a simulation tool for hypothesis testing.

Note: Intro -- Title -- Contents -- Make your own submarine -- Create a cloud in a bottle -- Levitating ping pong balls -- Rice quicksand -- Homemade litmus test -- Iron out your cereal -- Hurricane in a bottle -- Is that egg hard-boiled? -- Measure the speed of light with margarine -- Freeze a soft drink, instantly -- Chip bag/crisp packet fireworks -- Homemade fiber optics -- Soft drink volcano -- Toaster-powered hot-air balloon -- Use your loaf-how to make bread taste sweet -- Why is the sky blue? -- Extract DNA from a kiwifruit -- How to fool your senses -- Electric slime -- The chemistry of coppers -- Make your own water strider/pond skater -- Make your own magnifying glass -- Homemade lava lamp -- Strange glows from sugar -- The mysterious sound of an oven shelf -- Just chill out… your glow-stick -- Tangtastic: make your own fizzy candy -- Plant hydraulics, and why slugs and salt don't mix -- Hang an ice cube from a thread -- Liquid behaving badly -- Invisibility cloak -- Turn milk into cheese -- Is that really orange? -- Homemade mini fire extinguisher -- How to make a force field -- Invisible ink -- Seeing the invisible -- The world's cheapest camera -- Images from a magnifying glass -- Confuse your balance -- DIY Butter -- Boiling yogurt containers -- Flying tubes -- Make your own Gulf Stream -- Music from a wine glass -- The science of tidal waves -- The science of pool -- The science of bells and coffee cups -- Jam jars and flywheels -- Waterproof hanky -- Copyright.

Note: Alternative pre-mRNA Splicing: Theory and Protocols -- Contents -- Preface -- List of Abbreviations -- List of Contributors -- Part One: Theory -- 1 Splicing in the RNA World -- 1.1 Introduction: The Fascination of Alternative Pre-mRNA Splicing -- 1.2 RNA Can Adopt a Flexible Conformation -- 1.3 Enzymatic RNAs and the RNA World -- 1.4 Common Classes of Eukaryotic RNA -- 1.5 Alternative Pre-mRNA Splicing as a Central Element of Gene Expression -- 1.6 Increasing Numbers of Human Diseases are Associated with .Wrong. Splice Site Selection -- References -- 2 RNPs, Small RNAs, and miRNAs -- 2.1 Introduction -- 2.2 Ribonuclease P (RNase P) -- 2.3 Small Nucleolar RNAs (snoRNAs) -- 2.4 Small Regulatory RNAs -- 2.4.1 Short Interfering RNAs (siRNAs) -- 2.4.2 MicroRNAs (miRNAs) -- 2.4.3 Piwi-Interacting RNAs (piRNAs) -- 2.5 7SL RNA -- 2.6 7SK RNA -- 2.7 U-Rich Small Nuclear RNAs (U snRNAs) -- References -- 3 RNA Elements Involved in Splicing -- 3.1 Introduction -- 3.2 Splice Site Sequence -- 3.3 Intron/Exon Architecture -- 3.4 Splicing Regulatory Elements (SREs) -- 3.5 RNA Secondary Structure -- 3.6 Coupling between Transcription and RNA Processing -- 3.7 Combinatorial Effects of Splicing Elements -- References -- 4 A Structural Biology Perspective of Proteins Involved in Splicing Regulation -- 4.1 Introduction -- 4.2 The RRM: A Versatile Scaffold for Interacting with Multiple RNA Sequences and also Proteins -- 4.2.1 RRM-RNA Interaction and Splicing Regulation -- 4.2.1.1 RNA Binding by Splicing Factors Containing a Single RRM -- 4.2.1.2 RNA Binding by Splicing Factors Containing Multiple RRMs -- 4.2.2 RRM-RRM and RRM-Protein Interactions in Splicing Regulation -- 4.2.2.1 RRM-Protein Interactions Without RNA Binding -- 4.2.2.2 RRM-Protein Interactions Allowing RNA Binding -- 4.2.2.3 Impact of RRM-RRM Interactions on Splicing Mechanism. , 4.3 The Zinc Finger Domain -- 4.4 The KH Domain -- 4.5 Conclusions and Perspectives -- References -- 5 The Spliceosome in Constitutive Splicing -- 5.1 Introduction -- 5.2 The Mechanism of Splicing -- 5.3 The Stepwise Assembly Pathway of the Spliceosome -- 5.4 Dynamics of the Spliceosomal RNA-RNA Rearrangements -- 5.5 Splice-Site Recognition and Pairing Involves the Coordinated Action of RNA and Proteins -- 5.6 Driving Forces and Molecular Switches Required During the Spliceosome.s Activation and Catalysis -- 5.7 A Conformational Two-State Model for the Spliceosome.s Catalytic Center -- 5.8 Compositional Dynamics and Complexity of the Spliceosome -- 5.9 Reconstitution of Both Steps of S. cerevisiae Splicing with Purified Spliceosomal Components -- 5.10 Evolutionarily Conserved Blueprint for Yeast and Human Spliceosomes -- 5.11 Concluding Remarks -- References -- 6 The Use of Saccharomyces cerevisiaeto Study the Mechanism of pre-mRNA Splicing -- 6.1 Introduction -- 6.2 The Basics of Splicing -- 6.3 Yeast Intron-Exon Organization -- 6.4 The Yeast Spliceosome -- 6.5 Defining the Constellation of Yeast Splicing Factors: Primary Screens and Genomic Inspection -- 6.6 Reporter Genes as Readouts of Splicing Efficiency -- 6.7 Genetic Interaction: Dosage Suppression or Antagonism -- 6.8 Extragenic Suppressors -- 6.9 Synthetic Lethality -- 6.10 Systematic Approaches to Define the Interactome -- References -- 7 Challenges in Plant Alternative Splicing -- 7.1 Introduction -- 7.2 Plant Introns -- 7.3 The Plant Spliceosome -- 7.4 Plant Spliceosomal Proteins -- 7.5 Alternative Splicing in Plants -- References -- 8 Alternative Splice Site Selection -- 8.1 Introduction -- 8.2 The Players: Splicing Regulators -- 8.3 The Stage: The Splicing Complex Assembly and Exon Definition -- 8.4 Switching Splicing Patterns. , 8.5 SrcN1 Exon: A Model of Combinatorial Splicing Regulation -- 8.6 The Global View: Towards a Splicing Code -- References -- 9 Integration of Splicing with Nuclear and Cellular Events -- 9.1 Introduction -- 9.2 Overview -- 9.3 Nuclear Structure and Distribution of Splicing Factors -- 9.3.1 Cajal Bodies (CBs) -- 9.3.2 Splicing Factor Compartments (SFCs)/Speckles -- 9.3.3 Paraspeckles -- 9.4 Integration of Splicing with Nuclear and Cellular Processes -- 9.4.1 Splicing and Transcription -- 9.4.2 Splicing and mRNA Capping -- 9.4.3 Splicing and 30 End Processing -- 9.4.4 Splicing and Export -- 9.4.5 Splicing and Translation -- 9.4.6 Splicing and Nonsense-Mediated Decay (NMD) -- 9.4.7 Splicing and Chromatin Structure -- References -- 10 Splicing and Disease -- 10.1 Introduction -- 10.2 Splicing and Disease -- 10.3 Therapeutic Approaches -- 10.4 The Generation of Aberrant Transcripts -- 10.5 Exon Skipping -- 10.6 Cryptic Splice Site Activation -- 10.7 Intron Retention -- 10.8 Pseudoexon Inclusion -- 10.9 Unexpected Splicing Outcomes Following the Disruption of Classical Splicing Sequences -- 10.10 Conclusions -- References -- 11 From Bedside to Bench: How to Analyze a Splicing Mutation -- 11.1 Introduction -- 11.2 From Clinical Evaluation to Mutation Testing -- 11.3 An Example of an Uncertain Diagnosis -- 11.4 Mutation Testing Procedures -- 11.4.1 In-VitroSplicing -- 11.4.2 Minigene Splicing -- 11.5 Concluding Remarks -- References -- Part Two: Basic Methods -- 12 Analysis of Common Splicing Problems -- 12.1 Introduction -- 12.2 Is a Mutation Causing a Change in AS? -- 12.3 How is a Splicing Event Regulated, and How Can it be Influenced? -- 12.4 Is There a Difference in Alternative pre-mRNA Processing Between Two Cell Populations? -- References -- 13 Ultracentrifugation in the Analysis and Purification of Spliceosomes Assembled In Vitro. , 13.1 Theoretical Background -- 13.2 Protocol -- 13.2.1 Preparation of the Gradient -- 13.2.1.1 Manual Gradient Formation -- 13.2.1.2 Automatic Gradient Formation with the Gradient Master -- 13.2.2 Preparing the Run -- 13.2.2.1 Loading the Sample -- 13.2.2.2 Sedimentation Markers -- 13.2.3 The Ultracentrifuge Run -- 13.2.4 Harvesting the Gradient -- 13.3 Example Experiment -- 13.3.1 Purification of the Spliceosomal B Complex -- 13.3.1.1 Preliminaries -- 13.3.1.2 Preparation of the Spliceosomal B Complex -- 13.4 Troubleshooting -- References -- 14 Chemical Synthesis of RNA -- 14.1 Theoretical Background -- 14.1.1 RNA Solid-Phase Synthesis -- 14.1.2 RNA Modifications -- 14.1.2.1 RNA Modification During Solid-Phase Synthesis -- 14.1.2.2 Post-Synthetic RNA Modification -- 14.1.3 Combined Chemical and Enzymatic Strategies -- 14.2 Representative Protocols -- Protocol 1: Incorporation of Modified Phosphoramidites During Solid-Phase Synthesis -- Protocol 2: Coupling of Biophysical Probes to Aliphatic Amino Groups on RNA -- Protocol 3: Enzymatic Ligation of RNA fragments using T4 RNA or T4 DNA Ligase -- 14.3 Troubleshooting -- References -- 15 RNA Interference (siRNA, shRNA) -- 15.1 Theoretical Background -- 15.1.1 RNAi -- 15.1.2 siRNAs and shRNAs -- 15.1.3 Lentiviral-Mediated RNAi -- 15.2 Protocol -- 15.2.1 Map of pLKO.1 Puro -- 15.2.2 Oligonucleotide Design -- 15.2.2.1 Determining the Optimal 21-mer Targets in the Gene -- 15.2.2.2 Ordering Oligos Compatible with pLKO.1 -- 15.2.3 Generating the pLKO.1 Puro with a shRNA Construct -- 15.2.3.1 Annealing of the Oligonucleotides -- 15.2.3.2 Preparation of pLKO.1 TRC for Cloning -- 15.2.3.3 Ligating and Transforming into Bacteria -- 15.2.3.4 Screening for Inserts -- 15.2.4 Production of Lentiviral Particles -- 15.2.5 Lentiviral Infection -- 15.3 Example Experiment -- 15.4 Troubleshooting -- References. , 16 Expression and Purification of Splicing Proteins -- 16.1 Theoretical Background -- 16.2 Protocol 1: The Preparation of Total HeLa SR Proteins -- 16.2.1 Example Experiment -- 16.2.2 Troubleshooting and Important Points -- 16.3 Protocol 2: The Purification of Individual SR Proteins -- 16.3.1 Expression of SR Proteins in Escherichia coliand Purification -- 16.3.2 Preparation of SR Proteins Using a Baculovirus System -- 16.3.3 Example Experiment -- 16.3.4 Troubleshooting and Important Points -- 16.3.5 Production and Purification of Individual SR Proteins in Mammalian Cells -- References -- 17 Detection of RNA-Protein Complexes by Electrophoretic Mobility Shift Assay -- 17.1 Theoretical Background -- 17.1.1 Choice of RNA Substrate -- 17.1.2 Detection and Quantitation of Binding -- 17.1.3 Fluorescence -- 17.1.4 Chromogenic and Chemiluminescent Detection Methods -- 17.1.5 Stability of RNA-Protein Complexes During Electrophoresis -- 17.1.6 Competing Nucleic Acids and Polyanions -- 17.1.7 Binding Stoichiometry -- 17.1.8 Measurement of Binding Activity -- 17.1.9 Measurement of Dissociation Constants -- 17.1.10 Binding Competition -- 17.2 Protocol -- 17.2.1 Equipment -- 17.2.2 Reagents -- 17.2.3 Gel Preparation -- 17.2.4 Pre-Electrophoresis -- 17.2.5 Sample Preparation -- 17.2.6 Electrophoresis and Imaging -- 17.3 Example Experiment -- 17.4 Troubleshooting -- References -- 18 Functional Analysis of Large Exonic Sequences Through Iterative In VivoSelection -- 18.1 Theoretical Background -- 18.1.1 Spinal Muscular Atrophy -- 18.2 Protocol -- 18.2.1 Minigene, Cell Culture, Transfection, and In VivoSplicing Assay -- 18.2.2 Generation of a Partially Random Exon -- 18.2.3 In vivo Selection -- 18.2.4 Analysis of Sequences -- 18.3 Example Experiment -- 18.3.1 Generating the Initial Pool of Splicing Cassettes -- 18.3.2 In VivoSelection Procedure. , 18.4 Troubleshooting.

Note: Intro -- Non-Liability Statement -- Foreword -- A Message from the Sierra Club -- Introduction -- Important Information about the Tillamook and Clatsop State Forests Hikes.