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  • 1
    Publication Date: 2024-04-20
    Description: Excessive anthropogenic nitrogen (N) inputs to the biosphere have disrupted the global nitrogen cycle. To better quantify the spatial and temporal patterns of anthropogenic N enrichments, assess their impacts on the biogeochemical cycles of the planet and other living organisms, and improve nitrogen use efficiency (NUE) for sustainable development, we develop a comprehensive and synthetic dataset for anthropogenic N inputs to the terrestrial biosphere. This Harmonized Anthropogenic N Inputs (HaNi) dataset takes advantage of different data sources in a spatiotemporally consistent way to generate a set of high-resolution gridded N input products from the preindustrial to present (1860-2019). The HaNi dataset includes annual rates of synthetic N fertilizer, manure application/deposition, and atmospheric N deposition in cropland, pasture, and rangeland at 5-arcmin. Specifically, the N inputs are categorized, according to the N forms and the land use, as 1) NH4-N fertilizer applied to cropland, 2) NO3-N fertilizer applied to cropland, 3) NH4-N fertilizer applied to pasture, 4) NO3-N fertilizer applied to pasture, 5) manure N application on cropland, 6) manure N application on pasture, 7) manure N deposition on pasture, 8) manure N deposition on rangeland, 9) NHx-N deposition, and 10) NOy-N deposition. The total anthropogenic N (TN) inputs to global terrestrial ecosystems increased from 29.05 Tg N yr-1 in the 1860s to 267.23 Tg N yr-1 in the 2010s, with the dominant N source changing from atmospheric N deposition (before the 1900s) to manure N (the 1910s-2000s), and to synthetic fertilizer in the 2010s. The proportion of synthetic NH4-N fertilizer increased from 64% in the 1960s to 90% in the 2010s, while synthetic NO3-N fertilizer decreased from 36% in the 1960s to 10% in the 2010s. Hotspots of TN inputs shifted from Europe and North America to East and South Asia during the 1960s-2010s. Such spatial and temporal dynamics captured by the HaNi dataset are expected to facilitate a comprehensive assessment of the coupled human-earth system and address a variety of social welfare issues, such as climate-biosphere feedback, air pollution, water quality, and biodiversity.
    Keywords: atmospheric deposition; Binary Object; Crop; fertilizer; File content; manure; nitrogen; Nitrogen Model Inter-Comparison Project; NMIP; pastures; rangeland
    Type: Dataset
    Format: text/tab-separated-values, 20 data points
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  • 2
    Electronic Resource
    Electronic Resource
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 57 (2001), S. o1007-o1009 
    ISSN: 1600-5368
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: The title compound, C20H26O5, or ludongnin B, which has been isolated from Rabdosia var lophanthoides, is composed of three six-membered rings and two five-membered rings. The isobenzofuran ring system couples almost orthogonally to the methanocyclohepta[c]pyran moiety through a spiro-C atom.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 57 (2001), S. o685-o687 
    ISSN: 1600-5368
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: The asymmetric unit of ludongnin consists of two molecules of C20H24O6. The hydroxyl group of each molecule participates in a hydrogen bond with the carbonyl oxygen of the δ-lactone moiety of a translationally related molecule, thus giving rise to the two symmetry-independent sets of infinite chains in the crystal, one of which is stretched along the c and another along the a axis of the crystal.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 57 (2001), S. o849-o851 
    ISSN: 1600-5368
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: The crystal structure of the title compound consists of discrete C27H44O8 and H2O molecules, linked by hydrogen bonding.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Optical resolution of racemic carboxylic acids containing a halogen atom was attempted with stereoselective esterificatiob by Celite-adsorbed hydrolases in organic solvents. As lipase OF 360 from Candida cylindracea was found to stereoselectively esterify 2-(4-chlorophenoxy)propanoic acid, the (R)-enantiomer (d-isomer) of which is an important herbicide, the effects of alcohol chain length on stereoselectivity as well as reaction rate were investigated. The results revealed that the alcohol chain length markedly affected the stereoselective esterification of 2-(4-chlorophenoxy)propanoic acid: longer-chain alcohols, such as tetradecanol, served as excellent substrates for optical resolution of the acid, although the reaction rate was moderate.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1573-4986
    Keywords: N-glycan synthesis ; GlcNAc-transferase ; transcription ; promoter ; housekeeping gene ; bp, base pair ; CAT, chloramphenicol acetyltransferase ; FBS, fetal bovine serum ; GnT, N-acetylglucosaminyltransferase ; LB, Luria broth ; kb, kilobases ; MEM, minimal essential medium ; MOPS, 3-[N-morphiolino]propanesulfonic acid ; PBS, phosphate-buffered saline ; PCR, polymerase chain reaction ; RACE, rapid amplification of cDNA ends ; RNase, ribonuclease ; SDS, sodium dodecyl sulfate ; TE, 10 mM Tris–HCl, pH 80 1 mM EDTA ; UDP-GlcNAc: alpha-6-D-mannoside beta-1 2-N-acetylglucosaminyltransferase ; II ; GnT II (EC 241143)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract UDP-GlcNAc: alpha-6-D-mannoside beta-1,2-N-acetylglucosaminyltransferase II (GnT II; EC 2.4.1.143) is essential for the normal assembly of complex Asn-linked glycans. Northern analysis showed a major transcript at 2.0 kb and a minor band at sim 2.9 kb in five different human cell lines. The gene (MGAT2) has three AATAAA polyadenylation sites at 68, 688 and 846 bp downstream of the translation stop codon. 3 prime-RACE (rapid amplification of cDNA ends) using RNA from the human cell line LS-180 indicated that all three sites were utilized for transcription termination. 5 prime-RACE and RNase protection analyses showed multiple transcription initiation sites at -440 to -489 bp relative to the ATG translation start codon (+1). The data show that the entire GnT II gene is on a single exon. The gene has a CCAAT box at -587 bp but lacks a TATA box and the 5 prime-untranslated region is GC-rich and contains consensus sequences suggestive of multiple binding sites for Sp1; these properties are typical for housekeeping genes. A series of chimeric constructs containing different lengths of the 5 prime-untranslated region fused to the chloramphenicol acetyltransferase (CAT) reporter gene were tested in transient transfection experiments using HeLa cells. The CAT activity of the construct containing the longest insert (1076 bp relative to the ATG start codon) showed a sim 38-fold increase as compared to that of the control. Removal of the region between -636 and -553 bp caused a dramatic decrease in CAT activity indicating this to be the main promoter region of the gene.
    Type of Medium: Electronic Resource
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  • 7
    Publication Date: 2021-02-17
    Description: Understanding and quantifying the global methane (CH4) budget is important for assessing realistic pathways to mitigate climate change. Atmospheric emissions and concentrations of CH4 continue to increase, making CH4 the second most important human-influenced greenhouse gas in terms of climate forcing, after carbon dioxide (CO2). The relative importance of CH4 compared to CO2 depends on its shorter atmospheric lifetime, stronger warming potential, and variations in atmospheric growth rate over the past decade, the causes of which are still debated. Two major challenges in reducing uncertainties in the atmospheric growth rate arise from the variety of geographically overlapping CH4 sources and from the destruction of CH4 by short-lived hydroxyl radicals (OH). To address these challenges, we have established a consortium of multidisciplinary scientists under the umbrella of the Global Carbon Project to synthesize and stimulate new research aimed at improving and regularly updating the global methane budget. Following Saunois et al. (2016), we present here the second version of the living review paper dedicated to the decadal methane budget, integrating results of top-down studies (atmospheric observations within an atmospheric inverse-modelling framework) and bottomup estimates (including process-based models for estimating land surface emissions and atmospheric chemistry, inventories of anthropogenic emissions, and data-driven extrapolations). For the 2008–2017 decade, global methane emissions are estimated by atmospheric inversions (a top-down approach) to be 576 TgCH4 yr􀀀1 (range 550–594, corresponding to the minimum and maximum estimates of the model ensemble). Of this total, 359 TgCH4 yr􀀀1 or 60% is attributed to anthropogenic sources, that is emissions caused by direct human activity (i.e. anthropogenic emissions; range 336–376 TgCH4 yr􀀀1 or 50 %–65 %). The mean annual total emission for the new decade (2008–2017) is 29 TgCH4 yr􀀀1 larger than our estimate for the previous decade (2000–2009), and 24 TgCH4 yr􀀀1 larger than the one reported in the previous budget for 2003–2012 (Saunois et al., 2016). Since 2012, global CH4 emissions have been tracking the warmest scenarios assessed by the Intergovernmental Panel on Climate Change. Bottom-up methods suggest almost 30% larger global emissions (737 TgCH4 yr􀀀1, range 594–881) than top-down inversion methods. Indeed, bottom-up estimates for natural sources such as natural wetlands, other inland water systems, and geological sources are higher than top-down estimates. The atmospheric constraints on the top-down budget suggest that at least some of these bottom-up emissions are overestimated. The latitudinal distribution of atmospheric observation-based emissions indicates a predominance of tropical emissions ( 65% of the global budget, 〈30 N) compared to mid-latitudes ( 30 %, 30–60 N) and high northern latitudes ( 4 %, 60–90 N). The most important source of uncertainty in the methane budget is attributable to natural emissions, especially those from wetlands and other inland waters. Some of our global source estimates are smaller than those in previously published budgets (Saunois et al., 2016; Kirschke et al., 2013). In particular wetland emissions are about 35 TgCH4 yr􀀀1 lower due to improved partition wetlands and other inland waters. Emissions from geological sources and wild animals are also found to be smaller by 7 TgCH4 yr􀀀1 by 8 TgCH4 yr􀀀1, respectively. However, the overall discrepancy between bottom-up and top-down estimates has been reduced by only 5% compared to Saunois et al. (2016), due to a higher estimate of emissions from inland waters, highlighting the need for more detailed research on emissions factors. Priorities for improving the methane budget include (i) a global, high-resolution map of water-saturated soils and inundated areas emitting methane based on a robust classification of different types of emitting habitats; (ii) further development of process-based models for inland-water emissions; (iii) intensification of methane observations at local scales (e.g., FLUXNET-CH4 measurements) and urban-scale monitoring to constrain bottom-up land surface models, and at regional scales (surface networks and satellites) to constrain atmospheric inversions; (iv) improvements of transport models and the representation of photochemical sinks in top-down inversions; and (v) development of a 3D variational inversion system using isotopic and/or co-emitted species such as ethane to improve source partitioning.
    Description: Published
    Description: 1561–1623
    Description: 6A. Geochimica per l'ambiente e geologia medica
    Description: JCR Journal
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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  • 8
    Publication Date: 2023-10-27
    Description: Nitrous oxide (N2O) is a long-lived potent greenhouse gas and stratospheric ozone-depleting substance, which has been accumulating in the atmosphere since the pre-industrial period. The mole fraction of atmospheric N2O has increased by nearly 25 % from 270 parts per billion (ppb) in 1750 to 336 ppb in 2022, with the fastest annual growth rate since 1980 of more than 1.3 ppb yr-1 in both 2020 and 2021. As a core component of our global greenhouse gas assessments coordinated by the Global Carbon Project (GCP), we present a global N2O budget that incorporates both natural and anthropogenic sources and sinks, and accounts for the interactions between nitrogen additions and the biochemical processes that control N2O emissions. We use Bottom-Up (BU: inventory, statistical extrapolation of flux measurements, process-based land and ocean modelling) and Top-Down (TD: atmospheric measurement-based inversion) approaches. We provide a comprehensive quantification of global N2O sources and sinks in 21 natural and anthropogenic categories in 18 regions between 1980 and 2020. We estimate that total annual anthropogenic N2O emissions increased 40 % (or 1.9 Tg N yr-1) in the past four decades (1980–2020). Direct agricultural emissions in 2020, 3.9 Tg N yr−1 (best estimate) represent the large majority of anthropogenic emissions, followed by other direct anthropogenic sources (including ‘Fossil fuel and industry’, ‘Waste and wastewater’, and ‘Biomass burning’ (2.1 Tg N yr−1), and indirect anthropogenic sources (1.3 Tg N yr−1). For the year 2020, our best estimate of total BU emissions for natural and anthropogenic sources was 18.3 (lower-upper bounds: 10.5–27.0) Tg N yr-1, close to our TD estimate of 17.0 (16.6–17.4) Tg N yr-1. For the period 2010–2019, the annual BU decadal-average emissions for natural plus anthropogenic sources were 18.1 (10.4–25.9) Tg N yr-1 and TD emissions were 17.4 (15.8–19.20 Tg N yr-1. The once top emitter Europe has reduced its emissions since the 1980s by 31 % while those of emerging economies have grown, making China the top emitter since the 2010s. The observed atmospheric N2O concentrations in recent years have exceeded projected levels under all scenarios in the Coupled Model Intercomparison Project Phase 6 (CMIP6), underscoring the urgency to reduce anthropogenic N2O emissions. To evaluate mitigation efforts and contribute to the Global Stocktake of the United Nations Framework Convention on Climate Change, we propose establishing a global network for monitoring and modeling N2O from the surface through the stratosphere. The data presented in this work can be downloaded from https://doi.org/10.18160/RQ8P-2Z4R (Tian et al. 2023).
    Type: Article , PeerReviewed
    Format: text
    Format: text
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  • 9
    Publication Date: 2013-11-27
    Description: Expression of KIAA0101 protein is associated with poor survival of esophageal cancer patients and resistance to cisplatin treatment in vitro Laboratory Investigation 93, 1276 (December 2013). doi:10.1038/labinvest.2013.124 Authors: Yao Cheng, Kang Li, Dongmei Diao, Kun Zhu, Lei Shi, Hao Zhang, Dawei Yuan, Qi Guo, Xuandi Wu, Di Liu & Chengxue Dang
    Keywords: chemotherapyesophageal cancerKIAA0101
    Print ISSN: 0023-6837
    Electronic ISSN: 1530-0307
    Topics: Medicine
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  • 10
    Publication Date: 2013-11-12
    Description: Background: Pouch moist snuff, as a form of oral smokeless tobacco products, is becoming increasingly popular in North America, Scandinavia (where it is known as Snus), South Asia and parts of Africa. User usually places a pouch between the upper jaw and cheek to obtain euphoria from tobacco, leading to partial intake of tobacco constituents. To evaluate user exposure to tobacco, an approach with a novel model mouth system was developed and applied to evaluate release of nicotine from the pouch. Results: A novel model mouth system has been developed to evaluate release behavior of tobacco constituents in pouch moist snuff. The system consists of the release medium reservoir module, the flow speed control module, the temperature control module, nicotine release module, and release solution collection module, and simulates buccal condition in terms of temperature, saliva compositions, and the rate of saliva production, etc. Artificial saliva was used as the release medium to evaluate release of nicotine in pouch moist snuff. The optimized test condition was that the release temperature of 37°C and the flow rate performed at 0.2 mL min-1 in the first 5 min and 0.1 mL min-1 in the next 55 min. The performance of the model mouth system was compared with in vivo data of nicotine release in human volunteers. Data from 23 brands of moist snuff indicated that nicotine release rates increased with extraction time and approximately 60-90% of nicotine was released after 30 min of extraction in most of the samples, and the release behavior of nicotine was affected by product weights, nicotine concentration, and product pH, etc. Conclusion: The model mouth system can be used to evaluate the release behavior of constituents in pouch moist snuff, especially those directly related to human health such as nicotine and tobacco specific nitrosamine (TSNA), etc. This indicated that the system is an alternative tool to evaluate user exposure to tobacco. With further testing and validation, the model mouth system can be applied in risk evaluation of smokeless tobacco products.
    Electronic ISSN: 1752-153X
    Topics: Chemistry and Pharmacology
    Published by Chemistry Central
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