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  • 1
    Electronic Resource
    Electronic Resource
    Ammonia assimilation in lupin nodules (1976)
    [s.l.] : Nature Publishing Group
    Nature 263 (1976), S. 703-705 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] We now report the formation of asparagine by a cell-free extract of the plant fraction of lupin nodules in the presence of ATP, aspartate, glutamine and Mg2+ (Table 1). Apparent Km values for aspartate and glutamine were determined to be 3.6 mM and 0.26 mM, respectively. Ammonia could replace ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/263703a0
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  • 2
    Electronic Resource
    Electronic Resource
    Organisation of nodulation and nitrogen fixation genes on a Rhizobium trifolii symbiotic plasmid (1984)
    Springer
    Archives of microbiology 139 (1984), S. 151-157 
    Details
    ISSN: 1432-072X
    Keywords: Rhizobium trifolii ; Symbiosis ; Nodulation ; Nitrogen fixation ; Symbiotic genes ; Reiterated sequences ; Plasmid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A Rhizobium trifolii symbiotic plasmid specific gene library was constructed and the physical organisation of regions homologous to nifHDK, nifA and nod genes was determined. These symbiotic gene regions were localised to u 25 kb region on the sym-plasmid, pPN1. In addition four copies of a reiterated sequence were identified on this plasmid, with one copy adjacent to nifH. No rearrangement of these reiterated sequences was observed between R. trifolii bacterial and bacteroid DNA. Analysis of a deletion derivative of pPN1 showed that these sequences were spread over a 110 kb region to the left of nifA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00401991
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  • 3
    Electronic Resource
    Electronic Resource
    The requirement for exopolysaccharide precedes the requirement for flavolan-binding polysaccharide in nodulation of Leucaena leucocephala by Rhizobium loti (1997)
    Springer
    Archives of microbiology 167 (1997), S. 182-186 
    Details
    ISSN: 1432-072X
    Keywords: Key words Exopolysaccharide ; Invasion ; Tannin ; Indeterminate nodules ; Lotus ; Leucaena ; Rhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Rhizobium loti strain PN4115 (NZP2213 str-1) ineffectively nodulates Leucaena leucocephala, i.e., strain PN4115 induces nodulation (Nod+) and is able to invade these nodules (Inv+), but fails to fix nitrogen (Fix–). Strain PN4115 does not synthesize a flavolan-binding polysaccharide (FBP), which is synthesized by the fully effective (Nod+Inv+Fix+) R. loti strain PN184 (NZP2037 str-1). The FBP may offer protection from prodelphinidin-rich flavolans synthesized by Lc. leucocephala. In this work, we show that exopolysaccharide (EPS)-negative mutants derived from strain PN4115 have a more severe ineffective phenotype (Nod+Inv–Fix–) on Lc. leucocephala than strain PN4115. This suggests that EPS from strain PN4115 is functional during invasion of Lc. leucocephala and that the requirement for EPS precedes the requirement for FBP.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050433
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  • 4
    Electronic Resource
    Electronic Resource
    Molecular cloning of a nodulation gene from fast- and slow-growing strains of Lotus rhizobia (1985)
    Springer
    Molecular genetics and genomics 201 (1985), S. 43-50 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Cosmids containing a nodulation gene from Rhizobium loti NZP2037 were isolated using a 12.8 kb nod:: Tn5 EcoRI fragment from the Nod- mutant strain PN233, as a hybridisation probe. A physical map of the nod region was established using the enzymes EcoRI and HindIII and the site of insertion of Tn5 in PN233 determined. Site-specific exchange of the cloned nod:: Tn5 fragment demonstrated that Tn5, and not an indigenous insertion sequence, was responsible for the nod mutation in PN233. The nod cosmids isolated complemented the Nod- phenotype of strain PN233 but restoration of the Fix phenotype was variable suggesting a need for marker rescue to occur before nitrogen fixation occurred. Corresponding nod cosmids were isolated from a R. loti strain, NZP2213, that forms ineffective tumour-like structures on Lotus pedunculatus and from the slow-growing strain (Bradyrhizobium sp), CC814s, by in planta complementation of PN233. Hybridisation experiments suggested that the nod gene region of R. loti NZP2037 was more homologous to Bradyrhizobium strain CC814s than with a nod gene region of R. trifolii strain PN100. However, transfer of the R. trifolii nod cosmid into the R. loti Nod mutant PN233, restored the ability of this strain to initiate nodules on Lotus pedunculatus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00397985
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  • 5
    Electronic Resource
    Electronic Resource
    Surrogate transformation of perennial ryegrass,Lolium perenne, using genetically modifiedAcremonium endophyte (1992)
    Springer
    Molecular genetics and genomics 233 (1992), S. 1-9 
    Details
    ISSN: 1617-4623
    Keywords: Acremonium sp. ; Transformation ; Chromosomal karyotype ; Surrogate grass transformation ; uidA andhph genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Conditions have been developed for transforming protoplasts of the perennial ryegrass endophyteAcremonium strain 187BB. Unlike most other ryegrass endophytes, this strain does not produce the lolitrem B neurotoxin and is therefore suitable as a host for surrogate introduction of foreign genes into grasses. Transformation frequencies of 700–800 transformants/μg DNA were obtained for both linear and circular forms of pAN7-1, a hygromycin (hph) resistant plasmid. Up to 80% of the linear transformants were stable on further culturing but only 25% of the circular transformants retained hygromycin resistance. Integration of pAN7-1 into the genome was confirmed by Southern blotting and probing of genomic digests of transformant DNA. Both single and tandemly repeated copies of the plasmid were found in the genome and both the number and sites of integration varied among the transformants. At least 13 chromosomes were identified in 187BB using contour-clamped homogeneous electric field (CHEF) gel electrophoresis. Probing of Southern blots of these gels confirmed that pAN7-1 had integrated into different chromosomes. The β-glucuronidase (GUS) gene,uidA, was also introduced into 187BB by co-transformation of pNOM-2 with pAN7-1. GUS activity was detected by growing the transformants on plates containing 5-bromo-4-chloro-3-indolyl β-D-glucuronic acid and by enzyme assays of mycelial extracts. Severalhph- anduidA-containing transformants were reintroduced into ryegrass seedlings and expression of GUS visualized in vivo, demonstrating that 187BB can be used as a surrogate host to introduce foreign genes into perennial ryegrass. Molecular analysis of fungal isolates from the leaf sheath confirmed that the pattern of pAN7-1 and pNOM-2 hybridizing fragments was identical to that observed in the fungus used as inoculum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00587554
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