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  • 1
    Online Resource
    Online Resource
    Geesthacht : Helmholtz-Zentrum Geesthacht, Zentrum für Material- und Küstenforschung
    Keywords: Forschungsbericht ; Nordsee ; Ostsee ; Windpark ; Offshore-Technik ; Umweltfaktor
    Type of Medium: Online Resource
    Pages: Online-Ressource (46 S., 1,97 MB) , Ill., graph. Darst., Kt. , 30 cm
    Series Statement: HZG report 2014, 6
    DDC: 551.462133
    RVK:
    RVK:
    Language: English , German
    Note: Systemvoraussetzungen: Acrobat reader. , Zsfassung in dt. und engl. Sprache. - Zsfassung in dt. Sprache u.d.T.: Der @Einfluss großer Offshore Windparks auf die Nord- und Ostsee : eine umfassende Literaturübersicht
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  • 2
    Keywords: Forschungsbericht ; Wasserverschmutzung ; Ölpest ; Umweltanalytik
    Type of Medium: Online Resource
    Pages: Online-Ressource (127 S., 6,21 MB) , Ill., graph. Darst.
    Language: German
    Note: Förderkennzeichen BMBF 03F0458A - 03F0458B , Unterschiede zwischen der elektronischen Ressource und dem gedruckten Dokument können nicht ausgeschlossen werden , Auch als gedr. Ausg. vorhanden , Systemvoraussetzungen: Acrobat reader.
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  • 3
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    Unknown
    PANGAEA
    In:  Supplement to: Fischer, Philipp; Schwanitz, Max; Loth, Reiner; Posner, Uwe; Brand, Markus; Schroeder, Friedhelm (2016): First year of the new Arctic AWIPEV-COSYNA cabled Underwater Observatory in Kongsfjorden, Spitsbergen. Ocean Science Discussions, 34 pp, https://doi.org/10.5194/os-2016-52
    Publication Date: 2023-03-16
    Description: A combined year round assessment of selected oceanographic data and a macrobiotic community assessment was performed from October 2013 to November 2014 in the littoral zone of the polar fjord systems Kongsfjorden on the west coast of Svalbard (Norway). A state of the art remote controlled cabled underwater observatory technology was used for daily vertical profiles of temperature, salinity and turbidity together with a stereo-optical assessment of the macrobiotic community, including fish. The results reveal a distinct seasonal cycle in total species abundances with a significantly higher total abundance and species richness during the polar winter when no light is available under water compared to the summer months when 24-h light is available. During the winter months, a temporally highly segmented community was observed with respect to species occurrence with single species dominating the winter community for restricted times. In contrast, the summer community showed an overall lower total abundance, as well as a significantly lower number of species. The study clearly demonstrates the high potential of cable connected remote controlled digital sampling devices, especially in remote areas, such as the polar fjord systems, with harsh environmental conditions and limited accessibility. A smart combination of such new digital ?sampling? methods with classic sampling procedures can provide a possibility to significantly extend the sampling time and frequency especially in remote and difficult to access areas. This can help to provide a sufficient data density and therefore statistical power for a sound scientific analysis without increasing the invasive sampling pressure in ecologically sensitive environments.
    Keywords: AWI_Coast; Coastal Ecology @ AWI
    Type: Dataset
    Format: application/zip, 2 datasets
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  • 4
    Publication Date: 2023-03-16
    Keywords: AWI_Coast; AWIPEV; AWIPEV_based; AWIPEV_UW-Observatory; Class; Coastal Ecology @ AWI; Comment; Conductivity; DATE/TIME; DEPTH, water; Fish; Fish, total length; Group; Kongsfjorden, Spitsbergen, Arctic; OBSE; Observation; Species; Temperature, water; Turbidity (Formazin Turbidity Unit)
    Type: Dataset
    Format: text/tab-separated-values, 222304 data points
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  • 5
    Publication Date: 2023-03-16
    Keywords: AWI_Coast; AWIPEV; AWIPEV_based; AWIPEV_UW-Observatory; Coastal Ecology @ AWI; Conductivity; DATE/TIME; DEPTH, water; Kongsfjorden, Spitsbergen, Arctic; OBSE; Observation; Salinity; Temperature, water; Turbidity (Formazin Turbidity Unit)
    Type: Dataset
    Format: text/tab-separated-values, 1781918 data points
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  • 6
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Biochemistry 34 (1995), S. 3942-3947 
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: Two fatty acid binding proteins (FABPs) were isolated from Swiss Webster mouse brains. Neither protein cross-reacted with antisera to recombinant liver L-FABP. One protein, designated brain H-FABP, migrated on tricine sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as a single band at 14.5 kDa with pl 4.9. Brain H-FABP bound NBD-stearic acid and cis-parinaric acid with KD values near 0.02 and 0.5 µM, respectively. Brain H-FABP cross-reacted with affinity-purified antisera to recombinant heart H-FABP. The second protein, mouse brain B-FABP, migrated on tricine SDS-PAGE gels as a doublet at 16.0 and 15.5 kDa with pl values of 4.5 and 4.7, respectively. Brain B-FABP bound NBD-stearic acid and cis-parinaric acid with KD values near 0.01 and 0.7 µM, respectively. The brain B-FABP doublet was immunoreactive with affinity-purified antibodies against recombinant mouse brain B-FABP, but not with affinity-purified antibodies against heart H-FABP. [3H]Oleate competition binding indicated that the two brain FABPs had distinct ligand binding specificities. Both bound fatty acids, fatty acyl CoA, and lysophosphatidic acid. Although both preferentially bound unsaturated fatty acids, twofold differences in specific saturated fatty acid binding were observed. Brain B-FABP and brain H-FABP represented 0.1 and 0.01% of brain total cytosolic protein, respectively. In summary, mouse brain contains two native fatty acid binding proteins, brain H-FABP and brain B-FABP.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Journal of neurochemistry 66 (1996), S. 0 
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: Previous studies examining age differences in membrane fluidity and cholesterol content have reported on the average or total change in membrane structure, respectively. However, a membrane consists of an exofacial leaflet and a cytofacial leaflet that differ in fluidity and cholesterol distribution. The purpose of the present experiments was to determine fluidity and cholesterol distribution of the exofacial and cytofacial leaflets of brain synaptic plasma membranes (SPMs) from 3–4-, 14–15-, and 24–25-month-old C57BL/6NNIA mice by using trinitrobenzenesulfonic acid (TNBS)-quenching techniques and fluorescent probes. The exofacial leaflet of SPMs from young mice was significantly more fluid compared with the cytofacial leaflet. The large difference in fluidity between the two leaflets was abolished in SPMs of the oldest age group. Total SPM cholesterol and the cholesterol-to-phospholipid molar ratio did not differ among the three different age groups of mice. However, considerable differences were observed in the distribution of cholesterol in the two SPM leaflets. The exofacial leaflet contained substantially less cholesterol than did the cytofacial leaflet (13 vs. 87%, respectively) in SPMs of young mice. This asymmetric distribution of cholesterol was significantly modified with increasing age. There was an approximately twofold increase in exofacial leaflet cholesterol in the oldest group compared with the youngest age group. Transbilayer fluidity and cholesterol asymmetry were altered in SPMs of older mice. This approach is a new and different way of viewing how aging modifies membrane structure. Age differences in SPM leaflet structure may be an important factor regulating activity of certain membrane proteins.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: Although lipids are essential to brain function, almost nothing is known of lipid transfer proteins in the brain. Early reports indicates cross-reactivity of brain proteins with antisera against two native liver sterol transfer proteins, sterol carrier protein-2 (SCP-2) and the liver form of fatty acid-binding protein (L-FABP). Herein, polyclonal antibodies raised against the recombinant liver sterol transfer proteins SCP-2 and L-FABP were used to identify the lipid transfer proteins in the brains of alcohol-treated and control mice. L-FABP was not detectable in brain of either control or chronic ethanol-treated mice. In contrast, SCP-2 not only was present, but its level was significantly (p 〈 0.05) increased 23 and 50%, respectively, in brain homogenates and synaptosomes of mice exposed to alcohol. To determine whether antibodies against the recombinant liver SCP-2 reflected true levels of SCP-2 in brain, the cDNA sequence for brain SCP-2 was isolated from a brain cDNA library. The mouse brain SCP-2 sequence was 99.99% identical to the mouse liver SCP-2 sequence. The translated sequence differed by only one amino acid, and the replacement was conservative. Thus, unlike the fatty acid binding proteins, the SCP-2 moieties of brain and liver are essentially identical. Polyclonal antibodies against acyl-CoA binding protein, a lipid-binding protein that does not bind or transfer sterol, showed that increased levels of brain SCP-2 with chronic ethanol consumption did not represent a general increase in content of all lipid transfer proteins. Changes in the amount of SCP-2 may contribute to membrane tolerance to ethanol.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 52 (1989), S. 0 
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: Alcohols, including ethanol, have a specific effect on transbilayer and lateral membrane domains. Recent evidence has shown that alcohols in vitro have a greater effect on fluidity of one leaflet as compared to the other. The present study examined effects of chronic ethanol consumption on fluidity of synaptic plasma membrane (SPM) exofacial and cytofacial leaflets using trinitrobenzenesulfonic acid (TNBS) labeling and differential polarized fluorometry of 1,6-diphenyl-1,3,5-hexatriene (DPH). Mice were administered ethanol or a control liquid diet for 3 weeks. Animals were killed and SPM prepared. The exofacial leaflet of SPM was significantly more fluid than the cytofacial leaflet in both groups, as indicated by limiting anisotropy of DPH. However, differences between the two leaflets were much smaller in the ethanol-treated group. Ethanol at concentrations seen clinically had a greater effect in vitro on the more fluid exofacial leaflet. This asymmetric effect of ethanol was significantly diminished in the exofacial leaflet of the ethanol-treated mice. Chronic ethanol consumption has a specific effect on membranes. Membrane functions that may be regulated by asymmetry of fluidity and lipid distribution may be altered by chronic ethanol consumption.
    Type of Medium: Electronic Resource
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