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  • 1
    Online Resource
    Online Resource
    New York, NY :Springer,
    Keywords: Cell receptors-Congresses. ; Electronic books.
    Type of Medium: Online Resource
    Pages: 1 online resource (542 pages)
    Edition: 1st ed.
    ISBN: 9781468446319
    Series Statement: Methodological Surveys in Biochemistry and Analysis Series ; v.13
    DDC: 571.6
    Language: English
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  • 2
    Electronic Resource
    Electronic Resource
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Plant Physiology 17 (1966), S. 27-46 
    ISSN: 0066-4294
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Plant Physiology 26 (1975), S. 441-481 
    ISSN: 0066-4294
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 34 (1978), S. 1433-1434 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary We report attempts to isolate and purify sialic acid-containing glycolipids (gangliosides) from etiolated hypocotyls of soybean (Glycine max) using methods developed for rat liver. The maximum amounts of ganglioside sialic acid present was found to be less than. 0.021 nmole/g fresh weight or less than 1:100,000 the amounts present in rat liver. We conclude that this tissue lacks gangliosides.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Histochemistry and cell biology 88 (1987), S. 17-22 
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Maize root tips were fixed in glutaraldehyde fixatives containing tannic acid and then processed for electron microscopy. Under these conditions, tannic acid selectively stained the contents of the Golgi apparatus secretory vesicles of some outer root cap cells, the cell walls of all cells, and substances in, and adjacent to, intercellular connections of mature primary walls and of secondary walls. Intercellular connections of the young primary walls were not stained. Plasma membranes, and substances associated with the outer leaflets of the plasma membranes, were also stained. Tannic acid-positive material was associated with the cell plate vesicles of forming walls but very little, or none, was associated with the Golgi apparatus vesicles of dividing cells.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An ultrastructural study of the pollen tubes of Lilium and Clivia has demonstrated three different classes of longitudinal structures which could influence patterns of protoplasmic streaming and/or serve as “guide elements” in the vectorial migration of secretory vesicles: (a), cortical and noncortical microtubules; (b), microfilaments; and (c), subcortical tubules and cisternae of the endoplasmic reticulum (“subsurface cisternae”). Morphological details of these structures are described. Colchicine concentrations which lead to the complete disappearance of the microtubules affect neither germination of the pollen nor cytoplasmic streaming and tip growth of the elongating pollen tubes. Tip growth is initially uninhibited by cycloheximide, and cytoplasmic streaming is insensitive to this inhibitor. However, both of these processes are sensitive to cytochalasin B and vinblastine. Our results suggest that neither microtubules nor subsurface cisternae are essential for cytoplasmic streaming and directional secretion of cell surface materials in the pollen tube but would be consistent with an involvement of microfilamentous structures in these processes. Additionally, the possible importance of the lateral cross-link elements interconnecting all three types of structures is discussed.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 132 (1972), S. 365-380 
    ISSN: 1432-0878
    Keywords: Golgi apparatus ; Membranes ; Cross-bridges ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Electron opaque cross-bridge structures span the inter- and intracisternal spaces and provide membrane-to-membrane connections between adjacent cisternae of dictyosomes of pollen tubes ofClivia andLilium. Additionally, the classic intercisternal rods, characteristic of intercisternal regions near the maturing face of dictyosomes, are connected with the adjacent membranes through similar cross-bridge elements. We suggest that these structural links are responsible for maintaining the flattened appearance of the central parts of Golgi apparatus cisternac as well as for the coherence of cisternae within the stack. Observations on other plant (e.g. microsporocytes ofCanna) and animal cells (e.g. rodent liver and hepatoma cells, newt spermatocytes) show that such an array of membrane cross-links is a universal feature of Golgi apparatus architecture. The cross-bridges appear as part of the complex “zone of exclusion” which surrounds dictyosomes, entire Golgi apparatus and Golgi apparatus equivalents in a variety of cell types.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1615-6102
    Keywords: Golgi apparatus ; Endoplasmic reticulum ; Polyribosomes ; Golgi apparatus-associated polyribosomes ; Wild carrot (Daucus carota)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Endoplasmic reticulum-polyribosome-Golgi apparatus associations were a general feature of cells of suspension cultures of wild carrot (Daucus carota L.). Free polyribosomes occurred within the Golgi apparatus zone for all dictyosomes and with equal frequency at all levels within the stack including the most mature or trans face. When evaluated and quantified from electron micrographs, approximately 60% of the dictyosome profiles were characterized by a system of transition elements consisting of part smooth-part rough endoplasmic reticulum. These were encountered most frequently in the immediate vicinity of the immature, forming or cis face, usually toward the periphery of the stacked cisternae. Analysis of serial sections showed that those dictyosome profiles not exhibiting this characteristic did so primarily because of an unfavorable plane of sectioning. All dictyosomes examined in 5 or more serial sections revealed some type of close association with endoplasmic reticulum. Some of the associations were so close that direct connections between Golgi apparatus and endoplasmic reticulum tubules could not be excluded. Also present, especially at the forming or cis face, were small 600 nm transition vesicles with nap-like surface coats on nearly 90% of the dictyosomes examined. More than 50% exhibited spiny (clathrin-)coated vesicles at the mature or trans face.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1615-6102
    Keywords: Pollen tube ; Endoplasmic reticulum ; Golgi apparatus ; Membrane biogenesis ; Plasma membrane (Tobacco)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Tobacco (Nicotiana tabacum L.) pollen, germinated 4 hours in suspension culture, was labeled with radioactive leucine and fractionated into constituent membranes by the technique of preparative free-flow electrophoresis. Tubes were ruptured by sonication directly into the electrophoresis buffer. Unfortunately, the Golgi apparatus of the rapidly elongating pollen tubes did not survive the sonication step. However, it was possible to obtain useful fractions of endoplasmic reticulum and mitochondria. To obtain Golgi apparatus, glutaraldehyde was added to the homogenization buffer during sonication. Plasma membrane, which accounted for only about 3% of the total membrane of the homogenates as determined by staining with phosphotungstate at low pH, was obtained in insufficient quantity and fraction purity to permit analysis. Results show rapid incorporation of [3H]leucine into endoplasmic reticulum followed by rapid chase out. The half-time for loss of radioactivity from the pollen tube endoplasmic reticulum was about 10 minutes. Concomitant with the loss of radioactivity from endoplasmic reticulum, the Golgi apparatus fraction was labeled reaching a maximum 20 minutes post chase. The findings suggest flow of membranes from endoplasmic reticulum to the Golgi apparatus during pollen tube growth.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1615-6102
    Keywords: Auxin ; Plasma membrane ; NADH oxidase ; (Semidehydroascorbate reductase) ; Soybean(Glycine max L.)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Purified plasma membrane vesicles isolated both by aqueous twoPhase methods and by free-flow electrophoresis from homogenates prepared in the presence of 10 mM ascorbate, oxidized external NADH at rates of about 15 nanomoles/min/mg protein. The rate in the isolated vesicles was accelerated, without perceptible lag, 1.5-to 2-fold by 1 to 10 μM auxin (2,4-dichlorophenoxyacetic acid or indole-3-acetic acid). The reaction would be expected to result in acidification of the vesicle interiors and is proposed as a mechanism to account for auxin-induced acidification of cytoplasmin vitro.
    Type of Medium: Electronic Resource
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