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  • 1
    Electronic Resource
    Electronic Resource
    Plastocyanin is encoded by a single-copy gene in the pea haploid genome (1989)
    Springer
    Plant molecular biology 12 (1989), S. 655-666 
    Details
    ISSN: 1573-5028
    Keywords: chloroplast ; presequence ; promotor ; light-responsive element ; Z-DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract cDNA clones for pea plastocyanin were isolated from a pea leaf cDNA library screened with a 32P-labelled mixed oligonucleotide probe predicted from part of the N-terminal amino acid sequence of pea plastocyanin. The six cDNA clones isolated were found to be identical in the regions in which they overlapped. A Southern blot of restricted pea DNA probed with one of these cDNA clones showed the pea plastocyanin gene to exist as a single copy in the haploid genome. A pea genomic library in λEMBL3 screened with the same cDNA clone gave three positive plaques which contained identical 16 kbp Bam HI fragments. A single uninterrupted plastocyanin gene was located near the middle of the fragment and was characterised by DNA sequencing. The derived amino acid sequence indicates that the plastocyanin precursor consists of 168 amino acid residues including a presequence of 69 amino acid residues. The transcription initiation site was located by S1 nuclease mapping approximately 50 bp upstream of the translation initiation site. A sequence similar to a consensus light-responsive element found in a large number of phytochrome-dependent light-inducible genes is located just upstream of the TATA box. A cluster of direct repeats containing potential Z-DNA-forming elements occurs 600–750 bp upstream of the transcription initiation site.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00044156
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Synthesis and accumulation of pea plastocyanin in transgenic tobacco plants (1990)
    Springer
    Plant molecular biology 14 (1990), S. 229-238 
    Details
    ISSN: 1573-5028
    Keywords: plastocyanin ; transgenic plants ; gene dosage ; chloroplast ; thylakoid lumen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The pea plastocyanin gene in a 3.5 kbp Eco RI fragment of pea nuclear DNA was introduced into tobacco by Agrobacterium-mediated transformation. Regenerated plants contained pea plastocyanin located within the chloroplast thylakoid membrane system. Analysis of seedlings from a self-pollinated transgenic plant containing a single copy of the pea plastocyanin gene indicated that seedlings homozygous for the pea gene contained almost twice as much pea plastocyanin as seedlings hemizygous for the pea gene. Homozygous seedlings contained approximately equal amounts of pea and tobacco plastocyanins. The amount of tobacco plastocyanin in leaves of transgenic plants was unaffected by the expression of the pea plastocyanin gene. The mRNA from the pea gene in tobacco was indistinguishable by northern blotting and S1 nuclease protection from the mRNA found in pea. In both pea and transgenic tobacco, expression of the pea plastocyanin gene was induced by light in leaves but was suppressed in roots. Pea plastocyanin free of contaminating tobacco plastocyanin was purified from transgenic tobacco plants and shown to be indistinguishable from natural pea plastocyanin by N-terminal protein sequencing and 1H NMR spectroscopy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00018563
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Embryo yield in wheat anther culture is influenced by the choice of sugar in the culture medium (1990)
    Springer
    Plant cell reports 9 (1990), S. 14-16 
    Details
    ISSN: 1432-203X
    Keywords: Triticum aestivum ; Anther culture ; Sugars ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effect of employing different sugars in wheat anther culture has been investigated using four Spring wheat cultivars. The most responsive cultivar, Orofen, gave a three to four-fold increase in embryo yield when maltose was used in place of sucrose, with 50 embryos being produced for every 100 anthers cultured. Measurement of sugar concentrations in the culture media indicated that sucrose was more rapidly hydrolysed than maltose. However, neither the osmotic potential of the medium nor the concentration of glucose appeared to be critical factors in determining embryo yield.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232126
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Expression of the Talaromyces flavus glucose oxidase gene in cotton and tobacco reduces fungal infection, but is also phytotoxic (1999)
    Springer
    Molecular breeding 5 (1999), S. 219-232 
    Details
    ISSN: 1572-9788
    Keywords: disease resistance ; glucose oxidase ; Gossypium hirsutum ; Nicotiana tabacum ; Talaromyces flavus ; transgenic plant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Glucose oxidase secreted by the fungus Talaromyces flavus generates, in the presence of glucose, hydrogen peroxide that is toxic to phytopathogenic fungi responsible for economically important diseases in many crops. A glucose oxidase gene from T. flavus, was modified with a carrot extensin signal peptide and fused to either a constitutive or root-specific plant promoter. T1 tobacco plants expressing the enzyme constitutively were protected against infection by the seedling pathogen Rhizoctonia solani. Constitutive expression in tobacco was associated with reduced root growth, and slow germination on culture medium, and with reduced seed set in glasshouse conditions. Several independent transformed cotton plants with a root-specific construct expressed high glucose oxidase activity in the roots, excluding the root tip. Selected T3 homozygous lines showed some protection against the root pathogen, Verticillium dahliae, but not against Fusarium oxysporum. High levels of glucose oxidase expression in cotton roots were associated with reduced height, seed set and seedling germination and reduced lateral root formation. If this gene is to be of value for crop protection against pathogens it will require precise control of its expression to remove the deleterious phenotypes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009625801909
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  • 5
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    The Effect of Blood Flow on Magnetic Resonance Imaging of Non Thermal Irreversible Electroporation (2013)
    Nature Publishing Group (NPG)
    Details
    Publication Date: 2013-10-31
    Description: To generate an understanding of the physiological significance of MR images of Non-Thermal Irreversible Electroporation (NTIRE) we compared the following MR imaging sequences: T1W, T2W, PD, GE, and T2 SPAIR acquired after NTIRE treatment in a rodent liver model. The parameters that were studied included the presence or absence of a Gd-based contrast agent, and in vivo and ex-vivo NTIRE treatments in the same liver. NTIRE is a new minimally invasive tissue ablation modality in which pulsed electric fields cause molecularly selective cell death while, the extracellular matrix and large blood vessels remain patent. This attribute of NTIRE is of major clinical importance as it allows treatment of undesirable tissues near critical blood vessels. The presented study results suggest that MR images acquired following NTIRE treatment are all directly related to the unique pattern of blood flow after NTIRE treatment and are not produced in the absence of blood flow. Scientific Reports 3 doi: 10.1038/srep03088
    Electronic ISSN: 2045-2322
    Topics: Natural Sciences in General
    Published by Nature Publishing Group (NPG)
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