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  • 1
    Digitale Medien
    Digitale Medien
    Dynamic simulations of water at constant chemical potential (1992)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 96 (1992), S. 1333-1342 
    Details
    ISSN: 1089-7690
    Quelle: AIP Digital Archive
    Thema: Physik , Chemie und Pharmazie
    Notizen: The grand molecular dynamics (GMD) method has been extended and applied to examine the density dependence of the chemical potential of a three-site water model. The method couples a classical system to a chemical potential reservoir of particles via an ansatz Lagrangian. Equilibrium properties such as structure and thermodynamics, as well as dynamic properties such as time correlations and diffusion constants, in open systems at a constant chemical potential, are preserved with this method. The average number of molecules converges in a reasonable amount of computational effort and provides a way to estimate the chemical potential of a given model force field.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1063/1.462169
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  • 2
    Digitale Medien
    Digitale Medien
    Crystallization and preliminary X-ray diffraction studies of human epidermal growth factor (2000)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 56 (2000), S. 62-63 
    Details
    ISSN: 1399-0047
    Quelle: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Thema: Chemie und Pharmazie , Geologie und Paläontologie , Physik
    Notizen: Human epidermal growth factor (hEGF), a 6.2 kDa protein of 53 amino acids with three internal disulfide bridges, has been crystallized by the hanging-drop method. hEGF crystallizes in space group P3121 (or P3221) using MgCl2 as precipitant, with unit-cell parameters a = b = 61.4, c = 87.0 Å. Another type of crystal, obtained using NaCl as precipitant, belongs to a tetragonal point group and has unit-cell dimensions a = b = 102.5, c = 166.6 Å. The trigonal crystals with the smaller unit cell diffract X-rays better and a native data set from a single crystal has been collected to 3.0 Å resolution.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1107/S0907444999012615
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  • 3
    Digitale Medien
    Digitale Medien
    Molecular-replacement studies of Trichosanthes kirilowii lectin 1: a structure belonging to the family of type 2 ribosome-inactivating proteins (2000)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 56 (2000), S. 1073-1075 
    Details
    ISSN: 1399-0047
    Quelle: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Thema: Chemie und Pharmazie , Geologie und Paläontologie , Physik
    Notizen: Trichosanthes kirilowii lectin 1 (TKL-1) isolated from the tuber of T. kirilowii consists of two chains, each with a molecular weight of about 30 kDa. It has immunological properties which are similar to some ribosome-inactivating proteins (RIPs). TKL-1 was crystallized in space group P212121 and diffraction data were collected to 2.7 Å resolution. The molecular-replacement method was applied to solve the structure, using different chains of ricin, abrin-a and trichosanthin as search models. A set of consistent solutions was further verified by Romit profile analysis. In addition, the spatial arrangement of the two chains of TKL-1 is identical to that of type 2 RIPs.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1107/S0907444900008192
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  • 4
    Digitale Medien
    Digitale Medien
    Arrestment response of the predatory mite Amblyseius longispinosus to Schizotetranychus nanjingensis webnests on bamboo leaves (Acari: Phytoseiidae, Tetranychidae) (2000)
    Springer
    Experimental and applied acarology 24 (2000), S. 227-233 
    Details
    ISSN: 1572-9702
    Schlagwort(e): arrestment ; behaviour ; predator-prey interaction ; spider mites
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The response of the predatory mite Amblyseius longispinosus (Acari: Phytoseiidae) to the webnest of the spider mite nanjingensis (Acari: Tetranychidae) was examined using two-choice tests in the laboratory. A. longispinosus females were found significantly more often on leaves with webnests than on leaves without webnests and were often observed searching under the webbing. Because spider mites and their eggs were removed from the webnests before experiments, predators responded to stimuli associated with webbing, mite feeding damage and other residues in the webnests.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1006384723313
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  • 5
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    Proteomic analysis on effectors involved in BMP-2-induced osteogenic differentiation of beagle bone marrow mesenchymal stem cells (2014)
    BioMed Central
    Details
    Publikationsdatum: 2014-03-02
    Beschreibung: ObjectiveTo identify the protein regulation profile of recombinant human bone morphogenetic protein-2 (rhBMP-2)-induced osteogenic differentiation in beagle bone marrow stem cells (BMSCs). Methods: Beagle BMSCs were isolated and cultured with or without rhBMP-2. Two-dimensional gel electrophoresis was used to determine the differences in protein expression in rhBMP-2-induced and non-induced BMSCs. Real-time PCR and western blotting analyses were used to verify the expression patterns of selected proteins. Results: After the induction, the osteogenic differentiation of beagle BMSCs was activated successfully. Nine and 11 proteins were found to be down- and up-regulated by rhBMP-2, respectively. The increase in Lim and SH3 domain protein 1(LASP1) and the decrease in ferritin were verified by real-time PCR and western blotting analyses. Conclusions: Among the 20 rhBMP-2-regulated factors, there is empirical evidence supporting the involvement of LASP1 and ferritin in osteogenic differentiation. LASP1 plays an important role in the regulation of the activity of the cytoskeleton, and ferritin is an important molecule in cellular iron homeostasis. Further studies focused on these 20 proteins will help elucidate the molecular mechanism(s) through which rhBMP-2 induces osteogenic differentiation of BMSCs.
    Digitale ISSN: 1477-5956
    Thema: Medizin
    Publiziert von BioMed Central
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  • 6
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    BCL2L10 inhibits growth and metastasis of hepatocellular carcinoma both in vitro and in vivo (2016)
    Wiley-Blackwell
    Details
    Publikationsdatum: 2016-10-23
    Beschreibung: ABSTRACT Background and Aims BCL2L10 is an apoptosis-related member of the BCL-2 protein family. The role of BCL2L10 in the pathogenesis of hepatocellular carcinoma (HCC) is poorly understood. This study was aimed to investigate the function and underlying mechanisms of BCL2L10 in HCC. Methods BCL2L10 expression in human HCC and corresponding adjacent normal tissues was investigated by quantitative real-time PCR and western blot. The biological functions of BCL2L10 in HCC cell lines were determined by cell viability, colony formation, cell apoptosis, cell cycle and cell metastasis assays, and in vivo by tumorigenicity and lung metastasis assays in nude mice. Human cancer pathway PCR array was employed to explore the genes regulated by BCL2L10 in HCC. Results BCL2L10 was down-regulated in human HCC tissues compared to their adjacent non-tumor tissues. Ectopic expression of BCL2L10 in HepG2 and Huh7 cells suppressed cell growth as evidenced by cell viability and colony formation assay, and induced cell apoptosis. HCC cells transfected with BCL2L10 revealed an increased cell proportion arrested at G2/M phase, concomitant with a reduction in the cell proportion in S-phase as compared with control cells. Additional, BCL2L10 repressed cell migration and angiogenesis. Over-expression of BCL2L10 also restrained the tumorigenecity and lung metastasis capacity in nude mice. The activation of JAK-STAT3 signaling was suppressed by BCL2L10 in HCC. Conclusion BCL2L10 was down-regulated in human HCC tissues compared to adjacent normal tissues. BCL2L10 suppressed HCC progression through inhibiting cell growth and metastasis. Thus, BCL2L10 functions as a tumor-suppressor in HCC. This article is protected by copyright. All rights reserved
    Print ISSN: 0899-1987
    Digitale ISSN: 1098-2744
    Thema: Medizin
    Publiziert von Wiley-Blackwell
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  • 7
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    Anomalous magnetic moments as evidence of chiral superconductivity in a Bi/Ni bilayer (2017)
    American Physical Society (APS)
    Details
    Publikationsdatum: 2017-08-29
    Beschreibung: Author(s): Junhua Wang, Xinxin Gong, Guang Yang, Zhaozheng Lyu, Yuan Pang, Guangtong Liu, Zhongqing Ji, Jie Fan, Xiunian Jing, Changli Yang, Fanming Qu, Xiaofeng Jin, and Li Lu There have been continuous efforts in searching for unconventional superconductivity in the past five decades. Compared to the well-established d -wave superconductivity in cuprates, the existence of superconductivity with pairing symmetries of other high angular momentum is less conclusive. Bi/Ni ep... [Phys. Rev. B 96, 054519] Published Mon Aug 28, 2017
    Schlagwort(e): Superfluidity and superconductivity
    Print ISSN: 1098-0121
    Digitale ISSN: 1095-3795
    Thema: Physik
    Publiziert von American Physical Society (APS)
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  • 8
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    [ASAP] Chiral Selenide-Catalyzed Enantioselective Allylic Reaction and Intermolecular Difunctionalization of Alkenes: Efficient Construction of C-SCF 3 Stereogenic Molecules (2018)
    American Chemical Society (ACS)
    Details
    Publikationsdatum: 2018-03-30
    Beschreibung: Journal of the American Chemical Society DOI: 10.1021/jacs.8b01513
    Print ISSN: 0002-7863
    Digitale ISSN: 1520-5126
    Thema: Chemie und Pharmazie
    Publiziert von American Chemical Society (ACS)
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  • 9
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    Inverse-Problem-Based Accuracy Control for Arbitrary-Resolution Fairing of Quasiuniform Cubic B-Spline Curves (2014)
    Hindawi
    Details
    Publikationsdatum: 2014-08-15
    Beschreibung: In the process of curves and surfaces fairing with multiresolution analysis, fairing accuracy will be determined by final fairing scale. On the basis of Dyadic wavelet fairing algorithm (DWFA), arbitrary resolution wavelet fairing algorithm (ARWFA), and corresponding software, accuracy control of multiresolution fairing was studied for the uncertainty of fairing scale. Firstly, using the idea of inverse problem for reference, linear hypothesis was adopted to predict the corresponding wavelet scale for any given fairing error. Although linear hypothesis has error, it can be eliminated by multiple iterations. So faired curves can be determined by a minimum number of control vertexes and have the best faring effect under the requirement of accuracy. Secondly, in consideration of efficiency loss caused by iterative algorithm, inverse calculation of fairing scale was presented based on the least squares fitting. With the increase of order of curves, inverse calculation accuracy becomes higher and higher. Verification results show that inverse calculation scale can meet the accuracy requirement when fitting curve is sextic. In the whole fairing process, because there is no approximation algorithm such as interpolation and approximation, faired curves can be reconstructed again exactly. This algorithm meets the idea and essence of wavelet analysis well.
    Print ISSN: 1024-123X
    Digitale ISSN: 1563-5147
    Thema: Mathematik , Technik allgemein
    Publiziert von Hindawi
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  • 10
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    Pressure effect on stabilities of self-Interstitials in HCP-Zirconium (2014)
    Nature Publishing Group (NPG)
    Details
    Publikationsdatum: 2014-07-19
    Beschreibung: The self-interstitial atoms (SIAs) mediate the evolution of micro-structures which is crucial in understanding the instabilities of hexagonal close packed (HCP) structures. Taking zirconium as a prototype, we investigate the pressure effect on the stabilities of SIAs using first-principles calculations based on density-functional theory. We found that the pressure greatly affects the stability of the SIAs. The SIAs in basal planes are more stable under pressure. The SIA configuration of the lowest formation energy changes from basal octahedral (BO) to octahedral (O) at a pressure of 21 GPa. The lowest formation enthalpy configuration switches from BO to S (split-dumbbell) at the pressure of 30 GPa. The formation volumes of SIAs decrease monotonically in response to an increase in pressure. Our results reveal that it is important to take pressure effects into account when predicting the micro-structural evolution of HCP structures. Scientific Reports 4 doi: 10.1038/srep05735
    Digitale ISSN: 2045-2322
    Thema: Allgemeine Naturwissenschaft
    Publiziert von Nature Publishing Group (NPG)
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