GLORIA — GEOMAR Library Ocean Research Information Access

Hits per page

hits 1 - 2 | 2 hits

Sorting

Electronic Resource

Genes for biosynthesis and assembly of CS3 pili of CFA/II enterotoxigenic Escherichia coli: novel regulation of pilus production by bypassing an amber codon (1989)

Jalajakumari, M. B. ; Thomas, C. J. ; Halter, R. ; [et al.]

Oxford, UK : Blackwell Publishing Ltd

Molecular microbiology 3 (1989), S. 0

add to mindlist on the mindlist

Details

ISSN: 1365-2958

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology , Medicine

Notes: The complete nucleotide sequence of the 4746bp HindIII fragment encoding the genes for the biosynthesis and assembly of CS3 pili has been determined. By site-directed mutagenesis in conjunction with analysis of the plasmid-encoded proteins in minicells, the actual reading frames for the various products have been determined. This demonstrated that the genes for four of the proteins (63 kD, 48 kD, 33 kD, and 20kD in size) are encoded entirely within the same open reading frame as a fifth protein (104kD). However, for synthesis of this latter protein, suppression or readthrough of an internal amber codon is required. Termination at this codon is also necessary for synthesis of the former proteins. Two further proteins are also encoded within the HindIII fragment: a 27 kD precursor of a periplasmic protein and the 17.5kD precursor of the major CS3 fimbrial subunit.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1365-2958.1989.tb00154.x

Permalink

	Location	Call Number	Limitation	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

Electronic Resource

Nucleotide sequence of ompV, the gene for a major Vibrio cholerae outer membrane protein (1986)

Pohlner, Johannes ; Meyer, Thomas F. ; Jalajakumari, M. B. ; [et al.]

Springer

Molecular genetics and genomics 205 (1986), S. 494-500

add to mindlist on the mindlist

Details

ISSN: 1617-4623

Keywords: Signal sequence ; Gene regulation ; Export ; Codon usage

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary The nucleotide sequence of the ompV gene of Vibrio cholerae was determined. The product of the gene is a 28,000 dalton protein which, after the removal of a 19 amino acid signal sequence, produces a mature outer membrane protein of 26,000 daltons. The cleavage site was determined by amino-terminal amino acid sequencing of the purified mature protein. The DNA upstream of the gene shows the presence of a typical promoter region as judged from the Escherichia coli consensus information; however, the Shine-Dalgarno sequence is associated with a region capable of forming a secondary structure in the mRNA. The formation of this structure would inhibit binding of the mRNA to the ribosome and reduce translation. It is proposed that this structure is recognized by a positive activator in V. cholerae and because of its absence in E. coli ompV is poorly expressed. The distribution of rare codons within ompV suggests that they may serve to slow down the translation of particular domains such that the nascent polypeptide has an opportunity to take up its conformation without interference from the later formed regions. Such a mechanism could aid localization of the protein if export were by a cotranslational secretion system.