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  • 1
    Online Resource
    Online Resource
    Verbundprojekt: PathControl : Teilprojekt: Wechselwirkung von Bacillus amyloliquefaciens FZB42 mit dem Pilzpathogen Rhizoctonia solani und der mikrobiellen Gemeinschaft bei der Kolonisierung von Wurzeln ; Projektlaufzeit: 01.01.2010 bis 30.06.2013 (2013)
    Neuherberg
    Details Availability
    Keywords: Forschungsbericht ; Salat ; Keimling ; Wurzel
    Type of Medium: Online Resource
    Pages: Online-Ressource (36 S., 5,37 MB) , Ill., graph. Darst.
    URL: https://edocs.tib.eu/files/e01fb15/81695576X.pdf
    URL: https://doi.org/10.2314/GBV:81695576X
    DOI: 10.2314/GBV:81695576X
    Language: German
    Note: Förderkennzeichen BMBF 0315654C. - Verbund-Nr. 01075466 , Unterschiede zwischen dem gedruckten Dokument und der elektronischen Ressource können nicht ausgeschlossen werden , Systemvoraussetzungen: Acrobat reader.
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  • 2
    Electronic Resource
    Electronic Resource
    Ammonium (methylammonium) transport by Azospirillum spp. (1982)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 15 (1982), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1982.tb00039.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Transport of the iron ionophore ferrichrome in Escherichia coli K-12 and Salmonella typhimurium LT2 (1981)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 11 (1981), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1981.tb06947.x
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Detection and in situ identification of representatives of a widely distributed new bacterial phylum (1997)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 153 (1997), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: 16S rRNA gene libraries were prepared by polymerase chain reaction amplification and cloning from soil samples taken periodically from a field with genetically modified plants. Sequence analyses of the cloned rDNAs indicated that 140 of them clustered apart from known bacterial phyla. Based on 31 full sequences a new phylum could be defined. It includes Holophaga foetida, ‘Geothrix fermentans’ and Acidobacterium capsulatum as the only cultured species so far. Therefore, this line of descent was named the Holophaga/Acidobacterium phylum. About 50 published partial sequences of cloned rDNAs retrieved from soil, freshwater sediments or activated sludge from different continents indicate the occurrence of further representatives of this phylum. Two specific hybridization probes were constructed for members of one of four subclusters. A careful data analysis revealed the importance and problems of identifying and dealing with artefacts such as chimeric structure when defining new phylogenetic groups based mainly upon cloned amplified rDNAs. For the first time, the presence of bacterial cells representing this group could be shown in soil, sediment, activated sludge and lake snow by in situ hybridization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1997.tb10480.x
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  • 5
    Electronic Resource
    Electronic Resource
    Ecology and evolution of bacterial microdiversity (2000)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology reviews 24 (2000), S. 0 
    Details
    ISSN: 1574-6976
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Using high resolution molecular fingerprinting techniques like random amplification of polymorphic DNA, repetitive extragenic palindromic PCR and multilocus enzyme electrophoresis, a high bacterial diversity below the species and subspecies level (microdiversity) is revealed. It became apparent that bacteria of a certain species living in close association with different plants either as associated rhizosphere bacteria or as plant pathogens or symbiotic organisms, typically reflect this relationship in their genetic relatedness. The strain composition within a population of soil bacterial species at a given field site, which can be identified by these high resolution fingerprinting techniques, was markedly influenced by soil management and soil features. The observed bacterial microdiversity reflected the conditions of the habitat, which select for better adapted forms. In addition, influences of spatial separation on specific groupings of bacteria were found, which argue for the occurrence of isolated microevolution. In this review, examples are presented of bacterial microdiversity as influenced by different ecological factors, with the main emphasis on bacteria from the natural environment. In addition, information available from some of the first complete genome sequences of bacteria (Helicobacter pylori and Escherichia coli) was used to highlight possible mechanisms of molecular evolution through which mutations are created; these include mutator enzymes. Definitions of bacterial species and subspecies ranks are discussed in the light of detailed information from whole genome typing approaches.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6976.2000.tb00564.x
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  • 6
    Electronic Resource
    Electronic Resource
    In situ localization of two methanotrophic strains in the rhizosphere of rice plants (1998)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 25 (1998), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Methanotrophic bacteria play an important role in regulating the methane flux from rice fields to the atmosphere. The abundance of methane-oxidizing bacteria is very heterogeneous along the rice root as revealed by most probable number (MPN) counts and by scanning confocal laser microscopy (SCLM). Two methanotrophic strains, Rp1 and Rp2, were isolated from high dilutions of MPN counts from the rhizoplane of rice roots grown in natural rice field soil. We used monoxenically grown rice plants to relocalize these strains on and in rice roots by means of polyclonal antisera and 16S rRNA probes. Strain Rp1 was even detected in xylem vessels. The two isolates also were able to recolonize roots grown in natural soil under competitive conditions. We found large colonies in deep grooves on the root surface between iron oxide precipitates. Some cells also were observed in the root cortex. The distribution of methane-oxidizing bacteria probably reflects the changing availability of methane and oxygen in the rhizosphere.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6941.1998.tb00465.x
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  • 7
    Electronic Resource
    Electronic Resource
    Production of auxin and other indolic and phenolic compounds by Paenibacillus polymyxa strains isolated from different proximity to plant roots (1997)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 22 (1997), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: We determined the actual (no tryptophan, Trp, addition) and potential (Trp 0.1 g l−1 added) production of indolic and phenolic compounds produced in different culture media by Paenibacillus polymyxa strains which had been isolated from different proximity to wheat roots. The production of indole-3-acetic acid (IAA) and related indolic metabolites by all strains tested was greatly enhanced upon Trp addition, suggesting that Trp is important as IAA precursor. A gradual decrease in potential IAA and an increase in potential indole-3-ethanol and indole-3-lactic acid production by strains isolated from non-rhizosphere soil (NRS) over rhizosphere soil (RS) to the rhizoplane (RP) were obtained. The NRS strain showed also a higher (potential) production of oxidized compounds such as indole-3-carboxylic and benzoic acid besides IAA. The determined metabolic differences indicate together with previous results on genetic and physiological parameters of P. polymyxa populations that plant roots select different P. polymyxa subpopulations. Our results suggest that the selected genotypes are differentially adapted to the decreasing oxygen pressure from NRS to RP. Possible effects of the probably microhabitat-specific metabolic profiles of the P. polymyxa isolates for plant growth are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6941.1997.tb00384.x
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  • 8
    Electronic Resource
    Electronic Resource
    Iron uptake and iron limited growth of Escherichia coli K-12 (1981)
    Springer
    Archives of microbiology 130 (1981), S. 353-356 
    Details
    ISSN: 1432-072X
    Keywords: Low affinity iron uptake ; Ferrichrome-and citrate-dependent iron transport ; Iron limited growth ; Iron content of Escherichia coli K-12
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cells of Escherichia coli K-12 could grow aerobically at an iron concentration as low as 0.05 μM without any of the known iron ionophores present. The growth rate increased between 0.05 and 2 μM iron. Supplementation with the iron ligands ferrichrome and citrate resulted in optimal growth already at 0.05 μM iron. Under certain conditions iron uptake preceded growth of cells by more than an hour. During logarithmic growth the rate of iron uptake matched the growth rate. The radioactive tracer method revealed a cellular iron content of 4 nmol/mg dry weight. After consumption of the iron in the medium cells continued to grow with high rate for 1–2 generations. The iron uptake activity was increased during iron starvation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00414599
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  • 9
    Electronic Resource
    Electronic Resource
    Flow-microcalorimetry measurements of aerobic and anaerobic soil microbial activity (1995)
    Springer
    Biology and fertility of soils 19 (1995), S. 203-208 
    Details
    ISSN: 1432-0789
    Keywords: Flow-microcalorimeter ; CO2 ; Cal/CO2 ratio ; N2O ; Aggregates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Heat output can be used as an indicator of microbial activity and is usually measured in a microcalorimeter with closed ampoules. In long-term experiments particularly, interpretation of the data is hindered by the changing environment in the closed ampoules because of O2 consumption and CO2 enrichment. We used a combination of a flow-microcalorimeter and a gas chromatograph to measure the heat flux and CO2 and N2O production rates under controlled conditions. Simultaneous detection of the heat output and CO2 emission allowed calculation of the calorimetric: CO2 (Cal/CO2) ratio. A mean ratio of-435 kJ mol-1 CO2 was detected in six different soils amended with glucose and incubated under aerobic conditions. This ratio indicated that CO2 was the end-product of catabolism. In wet 10–12 mm soil aggregates of a gleyic vertisol amended with glucose, values of-285 kJ mol-1 CO2 under an aerobic and-141 kJ mol-1 CO2 under a N2 atmosphere was determined. These findings indicated that fermentative metabolism occurred. The Cal/CO2 ratio was not affected when enough NO inf3 sup- was available and denitrification processes (N2O production) were possible.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00336160
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  • 10
    Electronic Resource
    Electronic Resource
    Control of nitrogenase inAzospirillum sp. (1991)
    Springer
    Plant and soil 137 (1991), S. 127-134 
    Details
    ISSN: 1573-5036
    Keywords: Key words ; ammonium ; Azospirillum sp. ; dinitrogenase reductase ; ‘switch off’
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Many N2-fixing organisms can turn off nitrogenase activity in the presence of NH4 + and turn it on again when the NH4 + is exhausted. One of the most interesting systems for accomplishing this is by covalent modification of one subunit of dinitrogenase reductase by dinitrogenase reductase ADP-ribosyltransferase (DRAT). The system can be reactivated when NH4 + is exhausted, by dinitrogenase reductase activating glycohydrolase (DRAG) which removes the inactivating group. It is fascinating that some species of the genusAzospirillum possess the DRAT and DRAG systems (A. lipoferum andA. brasilense), whereasA. amazonense in the same genus lacks DRAT and DRAG.A. amazonense responds to NH4 + but does not exhibit modification of dinitrogenase reductase characteristic of the action of DRAT. However, it has been possible to clone DRAT and DRAG and to introduce them intoA. amazonense, whereupon they become functional in this organism. The DRAT and DRAG system does not appear to function inAcetobacter diazotrophicus, an organism isolated from sugar cane, that fixes N2 at a pH as low as 3.0.A. diazotrophicus does show a rather sluggish response to NH4 +. A level of about 10 μM NH4 + is required to ‘switch off’ the system. The response to NH4 + is influenced by the dissolved oxygen concentration (DOC) as has been reported forAzospirillum sp. A DOC in equilibrium with 0.1 to 0.2 kPa O2 seems optimal for the response inA. diazotrophicus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02187443
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