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  • 1
    Online Resource
    Online Resource
    Proteine : Präparation, Gelelektrophorese, Membrantransfer und Immundetektion. (1997)
    Berlin, Heidelberg :Springer Berlin / Heidelberg,
    Details
    Keywords: Electronic books.
    Type of Medium: Online Resource
    Pages: 1 online resource (281 pages)
    Edition: 1st ed.
    ISBN: 9783642592270
    Series Statement: Springer Labormanuale Series
    URL: https://ebookcentral.proquest.com/lib/geomar/detail.action?docID=6853488
    Language: German
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    GEOMAR EBL
  • 2
    facet.materialart.
    Unknown
    TemBi 2014 mesocosm study: Summer storm impact on water chemistry and physics in Lake Stechlin (2021)
    PANGAEA
    Details
    Publication Date: 2024-03-22
    Description: We simulated an experimental summer storm in large-volume (~1200 m3, ~16m depth) enclosures in Lake Stechlin by mixing deeper water masses from the meta- and hypolimnion into the mixed layer (epilimnion). The mixing included the disturbance of a deep chlorophyll maximum (DCM) which was present at the same time of the experiment in Lake Stechlin and situated in the metalimnion of each enclosure during filling. Water physical variables and water chemistry was monitored for 42 days after the experimental disturbance event. Mixing disrupted the thermal stratification, increasing concentrations of dissolved nutrients and CO2 and changing light conditions in the epilimnion. Mixing, thus, stimulated phytoplankton growth, resulting in higher particulate matter concentrations of carbon, nitrogen and phosphorous.
    Keywords: Alkalinity, total; Ammonium; Ammonium molybdate reaction (FIASTAR 5000), (0.3 µm GF-75 prefiltered (Sterlitech)); BIBS; Bicarbonate ion; Bridging in Biodiversity Science; Calcium; Calcium carbonate; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, particulate; Carbon, organic, dissolved; Carbon, organic, particulate; Carbon, total, particulate; Carbonate ion; Carbon dioxide; Carbon dioxide, partial pressure; Chloride; Climate change; Climate driven Changes in Biodiversity of Microbiota; Conductivity, electrolytic; cyanobacteria; DATE/TIME; Day of experiment; DCM; DEPTH, water; Depth of Secchi Disk; Description; Difference derived from TPC and PIC; disturbance; ELTRA-800 (0.3 µm GF-75 filters (Sterlitech)); enclosure; Enclosure experiment; Flow-injection analyzer; Flow-injection analyzer (0.3 µm GF-75 prefiltered (Sterlitech)); Fugacity of carbon dioxide in seawater; Germany; High temperature combustion, infra-red detection (Shimadzu), (0.3 µm GF-75 prefiltered (Sterlitech)); Infralyt 50 (SAXON Junkalor GmbH), (0.3 µm GF-75 filters (Sterlitech)); Ion chromatography (Dionex), (0.3 µm GF-75 prefiltered (Sterlitech)); lake; Lake_Stechlin; Magnesium; mesocosm; Mesocosm label; Nitrate; Nitrite; Nitrogen, total; Nitrogen, total, particulate; Nitrogen, total dissolved; NITROLIMIT; Oxygen; Oxygen/Nitrogen ratio; Oxygen saturation; Oxygen sensor, YSI6560; PAR sensor Li-193SA, LI-COR Inc.; Peroxodisulfate oxidation method; Flow-injection analyzer; 0.3 µm GF-75 filters (Sterlitech); pH; Phosphorus, reactive soluble; Phosphorus, total; Phosphorus, total, particulate; Potassium; Pressure, technical; Radiation, photosynthetically active; SD; Secchi disk; Silicate, dissolved; Sodium; Stickstofflimitation in Binnengewässern; Sulfate; summer storm; TemBi; Temperature, air; Temperature, water; Titration; Treatment; Vaisala Weather station WXT520; water chemistry; water physics
    Type: Dataset
    Format: text/tab-separated-values, 10528 data points
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    DATA PANGAEA
  • 3
    Electronic Resource
    Electronic Resource
    Isolation and characterization of an RNA-containing nuclear matrix from Tetrahymena macronuclei (1979)
    s.l. : American Chemical Society
    Biochemistry 18 (1979), S. 1782-1788 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00576a023
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    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Continuous monitoring of phytoplankton dynamics in Lake Balaton (Hungary) using on-line delayed fluorescence excitation spectroscopy (2005)
    Oxford, UK : Blackwell Science Ltd
    Freshwater biology 50 (2005), S. 0 
    Details
    ISSN: 1365-2427
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: 1. This study introduces delayed fluorescence (DF) excitation spectroscopy as an on-line tool for in situ monitoring of the composition and biomass of various colour classes of phytoplankton when they are photosynthetically active (cyanobacteria, chlorophytes, chromophytes and cryptophytes). The DF data are validated by comparison with those from conventional methods (weekly microscopic counts and the measurement of chlorophyll concentration).2. The composition of phytoplankton as assessed by DF agreed reasonably well with the results from microscopic counts, particularly when differences in chlorophyll-specific DF integrals of the various colour classes were taken into account.3. Integrals of DF spectra were converted into concentration of chlorophyll a using empirical factors derived from field data. The value of the conversion factor was nearly twice as high when the relative abundance of cyanobacteria was low (〈15%) than when it was high. The converted DF-chl time series agreed well with chlorophyll measurements particularly when blooms were developing. As the DF method is inherently free of the interference caused by pigment degradation products, the discrepancy between the two data sets increased during the collapse of blooms and when sediment resuspension was intense.4. Fourier spectrum analysis of the time series of DF-chl indicated that samples must be taken, at a minimum, every 2–3 days to capture the dynamics of phytoplankton. As a consequence, the dynamics of various algal blooms, including their timing, duration and net growth rate, could be estimated with greater confidence than by using conventional methods alone.5. On-line DF spectroscopy is an advanced technique for monitoring daily the biomass and composition of the photosynthetically active phytoplankton in aquatic environments, including turbid shallow lakes. At present, the detection limit is around 1 mg DF-chl a m−3 in terms of total biomass but confidence in estimates of phytoplankton composition declines sharply below about 5 mg chl a m−3.6. On-line DF spectroscopy represents a promising approach for monitoring phytoplankton. It will be useful in water management where it can act as an early-warning system of declines in water quality. In basic ecological research it can supplement manual methods. While default calibration spectra may be acceptable for routine monitoring, we suggest a careful individual calibration of the DF spectrometer for basic research. The statistical methods developed here help to assess the adequacy of various calibration sets.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2427.2005.01442.x
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    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    High performance liquid chromatography detection of phototrophic bacterial pigments in aquatic environments (1990)
    Springer
    Microbial ecology 19 (1990), S. 127-136 
    Details
    ISSN: 1432-184X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pigment extracts of phototrophic bacteria isolated from Lake Kinneret (Rhodopseudomonas palustris, Thiocapsa roseopersicina, Prosthecochloris aestuaris andChlorobium phaeobacteroides) were studied by means of high performance liquid chromatography (HPLC). An absorption wavelength of 360 nm provided the best resolution among the pigments of the species tested and between them and chlorophylla. Signature pigments were identified for each of these species, and their presence was thereby monitored in lake water samples.C. phaeobacteroides, which was observed in the anaerobic hypolimnion and predominated in the metalimnion, was recognized by a characteristic cluster of major chlorophyllous pigment peaks. The spectral qualities of these pigments were close but not identical to published data on bacteriochlorophylle, presumably due to the use of different solvents for extraction. The intensity of these pigment peaks was employed to determine the depth of the greatest phototrophic bacterial biomass, which was not related to that of algae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02012094
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    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Gonokokkenkulturen unter der Einwirkung von Penicillin (1949)
    Springer
    Archives of dermatological research 187 (1949), S. 590-604 
    Details
    ISSN: 1432-069X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Zusammenfassung An 12 kulturell gezüchteten Gonokokkenstämmen wurde deren Verhalten unter Penicillinzusatz in verschiedenen Konzentrationen untersucht. Dabei wurden folgende Beobachtungen gemacht: 1. In keinem Fall, selbst bei schwerst geschädigten Gonokokken traten Abweichungen von den gramnegativen Eigenschaften der Gonokokken auf. Bei subletalen Konzentrationen fiel lediglich eine blasse Färbung auf. 2. Mit steigender Penicillinkonzentration nimmt der Grad der Degenerationsformen zu. Es wurden die gleichen Entartungsformen beobachtet, wie sie auch in den Sekretabstrichen nach Penicillinbehandlung beschrieben werden. 3. Selbst bei subletalen Penicillinkonzentrationen wurden in jedem Falle neben den schwerst geschädigten noch normale Gonokokken gefunden. 4. Subkulturen auf normalen Nährböden von den im Wachstum gehemmten Kulturen wuchsen normal und charakteristisch weiter und trugen keine Zeichen mehr einer Schädigung weder im Plattenwachstum noch im mikroskopischen Bild. 5. Das Auftreten von Entartungsformen von Gonokokken ist keine spezifische Wirkung des Penicillins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00411843
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    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Electrochemical identification of microbially mediated hydrogen sulfide oxidation (1987)
    Springer
    Biogeochemistry 4 (1987), S. 15-26 
    Details
    ISSN: 1573-515X
    Keywords: process-monitoring ; biooxidation of hydrogen sulfide ; electroactivity ; phototrophic bacteria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract The process of H2S oxidation by the phototrophic bacteriaThiocapsa roseopersicina andChlorobium phaeobacteroides, respectively, was monitored using a Pt-glass-Ag0, Ag2S electrode combination without liquid junction. Due to the resulting pe(pH) and pH2S plottings three steps can be distinguished: oxidation of H2S to an S(0) state, oxidation of S (0) to SO4 2−, and oxidation of the remaining H2S directly to SO4 2−. Differences between the investigated bacteria exist with respect to their individual oxidation strategies.Thiocapsa apparently stops oxidizing H2S at pH2S 7.5 (e.g. 10−7.5M H2S) and shifts to the utilization of the intracellularly stored S (0). In contrastChlorobium utilizes its extracellularly stored sulfur parallel to the extracellular H2S fraction. The corresponding Pt-sensor responses (pe7 values) were found to be similar to the corresponding partial redox equilibria (pε7 values) of H2S oxidation stoichiometries as proposed by Van Niel (1931) and Trüper (1964). It is concluded that the recording of pe enables investigators to understand (and control) in situ redox processes, independent of their thermodynamic equilibration, only bound to changes of electroactivity vs. sensor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02187359
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    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Cytomembrane differentiation in a ciliate, Tetrahymena pyriformis (1971)
    Springer
    Cell & tissue research 119 (1971), S. 577-604 
    Details
    ISSN: 1432-0878
    Keywords: Endoplasmic reticulum ; Golgi apparatus ; Membrane flow ; Ciliate ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Various differentiating forms of rough and smooth endoplasmic reticulum (ER), as well as a novel type of a Golgi apparatus-equivalent organelle, are described for the ciliate Tetrahymena pyriformis. Ergastoplasmic stackings and dictyosomal configurations have been studied in particular detail since such structures had hitherto been reported not to occur in these ciliate cells. A Tetrahymena cell can contain several hundred dictyosomes, which are frequently formed at the mitochondrial jacket-ER. The following way of secretory flow is suggested for Tetrahymena: Small patches of rough ER (or of the nuclear envelope) loose their ribosomes and become smooth. From such smooth membrane areas vesicles pinch off which are distinct by greater membrane thickness and electron contrast as well as by an intimate association with characteristic granulo-fibrillar aggregates. Such ER-derived vesicles can then conflux, either to give rise to a stream of vesicles with distally increasing sizes, or to produce a localized “pile up” as more or less comprehensive stacks of flattened cisternae in the typical mode of a dictyosome with forming and secreting face polarity. The observations indicate that processes of cytomembrane differentiation and membrane flow take place in ciliate systems. In addition, the coincidence of vesicle pinching off with the appearance of fenestrae in the cisternae has led to new concepts of how pores in cisternae might generally be formed as a consequence of vesicle production.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00455249
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    Paper (German National Licenses)
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  • 9
    Electronic Resource
    Electronic Resource
    Cytomembrane differentiation in a ciliate, Tetrahymena pyriformis (1971)
    Springer
    Cell & tissue research 122 (1971), S. 244-253 
    Details
    ISSN: 1432-0878
    Keywords: Endoplasmic reticulum ; Membrane differentiation ; Tetrahymena pyriformis ; Ciliate ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Two special kinds of smooth surfaced differentiations of the endoplasmic reticulum (ER) of the ciliate Tetrahymena pyriformis are described. (A) A novel type of cytomembrane structure is represented by localized bifacial regions in which one side of the cisterna is studded with ribosomes, flexible in outline and of a cytomembraneous ultrastructure and the other side has a smooth, straight profile and a plasma membrane-like triple-layered appearance. Such smooth patches of predominantly rough ER-cisternae have a tendency to pair with a separation of ca. 250 Å. The micrographs suggest a participation of such patches in the formation of vesicles and/or dictyosomes. (B) Tubular structures, including those with microtubular as well as with “macrotubular” (300–650 Å) diameters, can be in continuity with ER profiles. Possible origins and functions of these tubular forms are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00337632
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    Paper (German National Licenses)
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  • 10
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    Unknown
    Iron-Coupled Anaerobic Oxidation of Methane Performed by a Mixed Bacterial-Archaeal Community Based on Poorly Reactive Minerals (2017)
    ACS Puplications
    In:  Environmental Science & Technology, 51 (21). pp. 12293-12301.
    Details
    Publication Date: 2020-08-28
    Description: Anaerobic oxidation of methane (AOM) was shown to reduce methane emissions by over 50% in freshwater systems, its main natural contributor to the atmosphere. In these environments iron oxides can become main agents for AOM, but the underlying mechanism for this process has remained enigmatic. By conducting anoxic slurry incubations with lake sediments amended with 13C-labeled methane and naturally abundant iron oxides the process was evidenced by significant 13C-enrichment of the dissolved inorganic carbon pool and most pronounced when poorly reactive iron minerals such as magnetite and hematite were applied. Methane incorporation into biomass was apparent by strong uptake of 13C into fatty acids indicative of methanotrophic bacteria, associated with increasing copy numbers of the functional methane monooxygenase pmoA gene. Archaea were not directly involved in full methane oxidation, but their crucial participation, likely being mediators in electron transfer, was indicated by specific inhibition of their activity that fully stopped iron-coupled AOM. By contrast, inhibition of sulfur cycling increased 13C-methane turnover, pointing to sulfur species involvement in a competing process. Our findings suggest that the mechanism of iron-coupled AOM is accomplished by a complex microbe-mineral reaction network, being likely representative of many similar but hidden interactions sustaining life under highly reducing low energy conditions.
    Type: Article , PeerReviewed
    Format: text
    DOI: 10.1021/acs.est.7b03126
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    OceanRep: Article in a Scientific Journal - peer-reviewed
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