Note: Front Cover -- Methods in Cell Biology, Volume 41 -- Copyright Page -- Contents -- Contributors -- Preface to the Second Edition -- Preface to the First Edition -- Chapter 1. Dissociation of Intact Cells from Tumors and Normal Tissues -- I. Introduction -- II. Application -- III. Methodology: Mechanical Cell Dispersion -- IV. Results -- V. Conclusion -- References -- Chapter 2. Assays of Cell Viability: Discrimination of Cells Dying by Apoptosis -- I. Introduction -- II. Changes in Light Scatter during Cell Death -- III. Cell Sensitivity to Trypsin and DNase -- IV. Fluorescein Diacetate (FDA) Hydrolysis and PI Exclusion -- V. Rh123 Uptake and PI Exclusion -- VI. PI Exclusion Followed by Counterstaining with Hoechst 33342 -- VII. Hoechst 33342 Active Uptake and PI Exclusion -- VIII. Controlled Extraction of Low MW DNA from Apoptotic Cells -- IX. Sensitivity of DNA in Situ to Denaturation -- X. Detection of DNA Strand Breaks in Apoptotic Cells -- XI. A Selective Procedure for DNA Extraction from Apoptotic Cells Applicable to Gel Electrophoresis and Flow Cytometry -- XII. Comparison of the Methods: Confirmation of the Apoptotic Mode of Cell Death -- References -- Chapter 3. Cell Preparation for the Identification of Leukocytes -- I. Introduction -- II. Antibodies -- III. Titering Antibodies -- IV. Third Color Reagents -- V. Cell Preparation -- VI. Labeling Procedures -- VII. Lysing Erythrocytes -- VIII. Detecting Dead Cells -- IX. Fixation -- X. Solutions and Reagents -- References -- Chapter 4. Multiparameter Analysis of Leukocytes by Flow Cytometry -- I. Introduction -- II. Correlated List-Mode Data -- III. Single-Label Analysis -- IV. Optical Filtration -- V. Compensation -- VI. Two-Color Fluorescence -- VII. Three-Color Immunophenotyping -- VIII. Fixing Cells -- IX. Detecting Dead Cells -- X. Summary -- References. , Chapter 5. Immunophenotyping Using Fixed Cells -- I. Introduction -- II. Application -- III. Materials -- IV. Cell Preparation and Staining -- V. Critical Aspects of the Procedure -- VI. Results and Discussion -- References -- Chapter 6. Simultaneous DNA Content and Cell-Surface Immunofluorescence Analysis -- I. Introduction -- II. Materials and Methods -- III. Instruments -- IV. Critical Aspects of the Method -- V. Results -- VI. Applications and Future Directions -- References -- Chapter 7. Flow Cytometry Crossmatching for Solid Organ Transplantation -- I. Introduction -- II. Applications -- Ill. Materials -- IV. Cell Preparation and Staining -- V. Critical Aspects of the Procedure -- VI. Standards -- VII. Instruments -- VIII. Results and Discussion -- References -- Chapter 8. Cell Membrane Potential Analysis -- I. Introduction -- II. Materials and Methods -- III. Improving Cytometry of Membrane Potentials -- References -- Chapter 9. Measurement of Intracellular pH -- I. Introduction -- II. Application -- Ill. Materials -- IV. Cell Preparation and Staining: BCECF or SNARF -- V. lnstruments -- VI. Critical Aspects -- VII. Results and Discussion -- References -- Chapter 10. Intracellular Ionized Calcium -- I. Introduction -- II. Flow Cytometric Assay With Indo-1 -- III. Use of Flow Cytometry and Fluo-3 to Measure [Ca2+]i -- IV. Use of Flow Cytometry and Fluo-3/Fura-Red for Ratiometric Analysis of [Ca2+]i -- V. Cell Conjugate Assays Combined with Calcium Analysis and Flow Cytometry -- VI. Pitfalls and Critical Aspects -- VII. Limitations -- VIII. Results -- References -- Chapter 11. Cellular Protein Content Measurements -- I. lntroduction -- II. Applications -- III. Materials -- IV. Cell Preparation and Staining -- V. Critical Aspects of the Procedure -- VI. Controls and Standards -- VII. Instruments -- VIII. Results and Discussion -- References. , Chapter 12. Lysosomal Proton Pump Activity: Supravital Cell Staining with Acridine Orange Differentiates Leukocyte Subpopulations -- I. Introduction -- II. Applications -- III. Materials -- IV. Staining Procedures -- V. Critical Aspects of the Procedure -- VI. Controls and Standards -- VII. Instruments -- VIII. Results -- References -- Chapter 13. Staining of DNA in Live and Fixed Cells -- I. Introduction -- II. Applications with DNA-Specific Fluorochromes -- III. Materials -- IV. Cell Preparation and Fixation -- V. Cell Staining -- VI. Critical Aspects -- VII. Controls and Standards -- VIII. Instruments -- IX. Results and Discussion -- References -- Chapter 14. High-Resolution Analysis of Nuclear DNA Employing the Fluorochrome DAPI -- I. Introduction -- II. Applications -- III. Materials -- IV. Cell Separation and Fixation Method -- V. Staining Procedure -- VI. Instrument Setup -- VII. Standards -- VIII. Results and Discussion -- References -- Chapter 15. Detergent and Proteolytic Enzyme-Based Techniques for Nuclear Isolation and DNA Content Analysis -- I. Introduction -- II. Basic Principles of the Methods -- III. Applications -- IV. Materials -- V. Methods -- VI. Results -- References -- Chapter 16. DNA Analysis from Paraffin-Embedded Blocks -- I. Introduction -- II. Application -- III. Methods -- IV. Critical Aspects of Technique -- V. Alternative Methods for Sample Preparation -- References -- Chapter 17. Controls, Standards, and Histogram Interpretation in DNA Flow Cytometry -- I. Introduction -- II. Application -- III. Instrument Quality Control and Standardization -- IV. DNA Histogram Interpretation -- V. Methods -- VI. Summary of Criteria for Interpretation of DNA Histograms -- References -- Chapter 18. DNA Content Histogram and Cell-Cycle Analysis -- I. Introduction -- II. DNA Content Histogram Basic Principles. , III. Cell-Cycle Analysis of DNA Content Histograms -- IV. Critical Aspects of DNA Content and Cell-Cycle Analysis -- V. Interpretation of DNA Content and Cell-Cycle Histograms -- VI. Conclusion -- References -- Chapter 19. Imniunochemical Quantitation of Bromodeoxyuridine: Application to Cell-Cycle Kinetics -- I. Introduction -- II. Applications -- III. Materials -- IV. Procedures -- V. Critical Aspects of the Procedure -- VI. Controls and Standards -- VII. Instruments -- VIII. Results -- IX. Summary -- References -- Chapter 20. Application and Detection of IdUrd and CldUrd as Two Independent Cell-Cycle Markers -- I. Introduction -- II. Application -- III. Materials -- IV. Methods -- V. Critical Aspects of the Procedure -- VI. Controls and Standards -- VII. Results -- References -- Chapter 21. Cell-Cycle Analysis Using Continuous Bromodeoxyuridine Labeling and Hoechst 33358-Ethidium Bromide Bivariate Flow Cytometry -- I. Introduction -- II. Application -- III. Materials -- IV. Procedures -- V. Controls and Standards -- VI. Instruments -- VII. Results -- References -- Chapter 22. Detection of BrdUrd-Labeled Cells by Differential Fluorescence Analysis of DNA Fluorochromes: Pulse-Chase and Continuous Labeling Methods -- I. Introduction -- 11. Applications -- 111. Materials -- IV. Cell Preparation and Staining Procedure -- V. Critical Aspects -- VI. Controls and Standards -- VII. Instruments -- VIII. Results -- References -- Chapter 23. Analysis of Intracellular Proteins -- I. Introduction -- II. Application -- III. Materials -- IV. Staining Procedures -- V. Critical Aspects -- VI. Controls and Standards -- VII. Instruments -- VIII. Multiparameter Analysis of Intracellular Antigens -- References -- Chapter 24. "Washless" Procedures for Nuclear Antigen Detection -- I. Introduction -- II. Applications -- Ill. Materials, Cell Preparation, and Staining. , IV. Critical Aspects of the Procedure: Controls, Standards, and Instruments -- V. Results -- References -- Chapter 25. Light Scatter of Isolated Cell Nuclei as a Parameter Discriminating the Cell-Cycle Subcompartments -- I. Introduction -- II. Application -- III. Materials -- IV. Critical Aspects of the Procedure -- V. Instruments -- Vl. Results -- VII. Appendix -- References -- Chapter 26. Simultaneous Analysis of Cellular RNA and DNA Content -- I. Introduction -- II. Applications -- III. Materials -- IV. Staining Procedures Employing A O -- V. Staining RNA and DNA with PY and Hoechst 33342 -- VI. Critical Aspects of the Procedures -- VII. Controls and Standards -- VIII. Instruments -- IX. Results -- X. Comparison of the Methods -- References -- Chapter 27. Analysis of DNA Content and Cyclin Protein Expression in Studies of DNA Ploidy, Growth Fraction, Lymphocyte Stimulation, and the Cell Cycle -- I. Introduction -- II. Bivariate Analysis of DNA Content versus Cyclin B1 or Cyclin E Expression -- III. Application of Cyclin B1 Analysis in DNA Ploidy and Chromatin Cycle Estimates -- IV. Lymphocyte Stimulation -- V. Tumor Growth Fraction Analysis -- Vl. Mapping the Cell Cycle: Mechanism of Action of Cytostatic Drugs -- VII. Critical Points of the Procedure -- VIII. Conclusions -- References -- Chapter 28. Oxidative Product Formation Analysis by Flow Cytometry -- I. Introduction -- II. Oxidative Burst -- III. Phagocytosis -- IV. Controls and Standards -- References -- Chapter 29. Flow Cytometric Determination of Cysteine and Serine Proteinase Activities in Living Cells with Rhodamine 110 Substrates -- I. Introduction -- II. Materials and Methods -- III. Results -- IV. Discussion -- References -- Chapter 30. Leucine Aminopeptidase Activity by Flow Cytometry -- I. Introduction -- II. Application -- III. Materials -- IV. Methods. , V. Critical Aspects of the Procedure.

Note: Intro -- Methods in Cell Biology VOLUME 102 -- Copyright -- Contents -- In Memoriam -- Contributors -- Preface To Fifth Edition -- Chapter - 1 - Introduction A: Recent Advances inCytometry Instrumentation, Probes, and Methods -- Abstract -- Preface -- Image Cytometry -- Seeing Is Believing -- Image Cytometry Applications -- New Instrumentations -- Multiparametric Capabilities of Image Cytometry -- The Merge of Systems -- Modifications of the Well-Known & -- ndash -- The Microcytometers -- Better & -- ndash -- Easier & -- ndash -- Affordable -- Off the Beaten Track & -- ndash -- Non-fluorescent Analyses -- Electrical Impedance Cytometry -- Raman Scatter Cytometry -- Mass-Spectrometry Cytometry -- New Probes, Components, and Methods -- Let There Be Light -- More Colorful World -- Revealing Cell Fates -- New Strategies for Data Analysis -- Perspective -- References -- SECTION - I - Down-sizing cytometry to "micro" dimension -- Chapter - 2 - Droplet Microfluidics for High-throughput Analysis of Cells and Particles -- Abstract -- Introduction -- Droplet Microfluidics -- Microchannel Characteristics -- Droplet Formation -- Particle Encapsulation -- Biocompatibility and Emulsion Lifetime -- Detection Techniques and Methodologies in Droplet Microfluidics -- High-Throughput Cell and Particle Analysis in Droplet Microfluidics -- Cell-Based Analysis -- Polymerase Chain Reaction, Particle Synthesis and Analysis -- Perspectives -- Conclusions -- References -- Chapter - 3 - Parallel Imaging Microfluidic Cytometer -- Abstract -- Introduction -- Background -- Flow Cytometry -- High-Content Screening -- HCS Instruments -- Instrument Design -- PMC System Architecture -- Robotics -- PMC Detector -- Data Processing -- Microfluidics -- Flow and Flow Focusing -- Sorting -- Operating Methods -- Microdevice Maintenance -- Sample Preparation. , Samples for the Primary-Cell (Lymphoma Model) Dilution Studies -- Cell Line for CPTHR Screen -- The cDNA Library for CPTHR Screen -- Results -- Sensitivity Trials on Primary B-cell Lymphoma Cells -- Dilution Study on Clonal Osteocytes -- Genome-Wide cDNA Screen -- Adding 1D Imaging to the PMC -- Classification of Phenotypes by 1D Images -- Confirmation of 1D Imaging on a PMC -- Proof of Principle for NT Assay by 1D Imaging -- Conclusions -- Acknowledgements -- References -- Chapter - 4 - Microfluidic Systems for Live Cell Imaging -- Abstract -- Introduction -- Physical Properties of Microfluidic Cell Culture -- Volume, Surface Area, and Cell/Medium Ratio -- Batch versus Continuous Reactors -- Laminar Flow -- Nutrient Exchange -- Shear Stress -- Material Interactions -- Microfabrication Methods -- PDMS Molding -- Master Template Fabrication -- Replicate Molding -- Packaging -- Flow Control -- Air Pressure-Driven Flow -- Manifold Interface -- Microincubation and Microscopy -- Gravity-Driven Flow -- Surface Tension Flow -- Design Aspects -- Plate Format -- Unit Layout -- Resistance Calculations -- Perfusion Barriers -- Cell Barriers -- Example Applications -- Microfluidic Culture Array -- Solution Switching -- Spatial Gradient Control -- 3D Extracellular Matrix Culture -- Nonadherent Cell Imaging -- Conclusion -- References -- Chapter - 5 - Rise of the Micromachines: Microfluidics and the Future of Cytometry -- Abstract -- Introduction -- The Smaller the Better: Microfluidics and Enabling Prospects for Single Cytomics -- Microflow Cytometry (& -- mu -- FCM) -- Microfluidic Cell Sorting (& -- mu -- FACS) -- Real-Time Cell Analysis: Living Cell Microarrays and a Real-Time Physiometry on a Chip -- Conclusions -- Acknowledgments -- References -- Chapter - 6 - Label-Free Resistive-Pulse Cytometry -- Abstract -- Introduction -- Resistive-Pulse Sensing. , Coulter Counter on a Chip -- Achieving nm Precision and High Throughput using Colloids -- Counting Cells with Microfluidic Coulter Counters -- Multiparametric RPS for Cell Cytometry -- Device Fabrication and Experimental Methods -- Functionalization of the Pore -- Estimate of the Density of Functionalized Antibody -- Fluid Handling, Data Acquisition, and Analysis -- Cell Size -- Cell-Surface Marker Screening -- Detection of CD34 in a Single Population of Cells -- Screening Cells in a Mixed Cell Population Based on the CD34 Receptor -- Cell Shape -- Applications -- Conclusion -- Acknowledgments -- References -- SECTION - II - Imaging cytometry -- Chapter - 7 - Laser Scanning Cytometry and Its Applications: A Pioneering Technology inthe Field of Quantitative Imaging Cytometry -- Abstract -- Introduction -- Definition of Quantitative Imaging Cytometry (QIC) and Key Features Distinguishing Imaging Cytometry Platforms -- Definition of QIC -- Key Features Distinguishing Imaging Cytometry Platforms -- Depth of Field -- Sample Illumination -- Signal Detection -- Imaging Mode: Spot Scanning, Widefield Illumination, or Both -- Type of Sample Labeling Required for Analysis: Fluorescent or Chromatic -- Imaging Cytometry Instrumentation Landscape -- Technical and Analytical Features of iGeneration Laser Scanning Cytometry -- Illumination Method and Depth of Field -- Signal Detection Method -- Autofocus -- Image and Data Acquisition -- Compensation -- Multiplexing -- Throughput and Variable-Resolution Scanning -- Basic Thresholding, Event Contouring, and Analytical Features -- Thresholding -- Event Contouring -- Multicomponent Event Contouring -- Random Sampling -- Analytical Features -- Data Display, Gating, and Relating Image and Cytometric Data -- iBrowser& -- reg -- Data Integration Software and iNovator Advanced Application Analysis Toolkit. , Analytical Performance Characteristics -- LSC Analysis Workflow -- Selected Application Areas of LSC -- Cell Cycle -- DNA Damage, Apoptosis, and Senescence -- Automated Tissue and TMA Analysis -- Cell Surface and Tissue Immunophenotyping -- Live-Cell Analysis -- Concluding Remarks -- References -- Chapter - 8 - Analytical Capabilities of the ImageStream Cytometer -- Abstract -- Introduction -- Origin of Imaging Flow Cytometry & -- ndash -- Historical Perspectives and Place Among Other Imaging Platforms -- Background -- Overview of the ImageStream Platform -- Methods -- Sample Preparation for Analysis by Imaging Cytometry -- Applications of ImageStream System -- Cell Death & -- ndash -- Apoptosis Quantification -- Cell Signaling & -- ndash -- Protein Translocation Analysis -- Molecule Colocalization and Trafficking -- Activation & -- ndash -- Change of the Cell Shape -- Identification of Stem and Progenitor Cells -- Other Applications -- Future Directions -- References -- Chapter - 9 - Laser Scanning Cytometry: Capturing the Immune System In situ -- Abstract -- Introduction -- Background: Laser Scanning Cytometry Technology forQuantitatively Imaging and Analyzing Immune Responses In situ -- Rationale for LSC Analysis of Antigen-Specific T Cell Responses In vitro and In vivo -- Detailed Protocols for Tracking Antigen-Specific T Cell Responses -- Analysis of Antigen-Specific T Cells In vitro -- Analysis of Antigen-Specific T Cells in Tissue In situ -- Preparation of Cell Suspensions for Adoptive Transfer and Immunization -- Harvesting and Preparation of Tissue -- Staining of Tissue Sections -- Acquisition and Analysis of Data Using WinCyte Software -- Acquisition and Analysis of Antigen Specific T Cells In vitro -- Acquisition and Analysis of Antigen Specific T Cells In situ. , Results: Analysis of the Role of pERK Signaling in Antigen-Specific Priming of T Cells -- Application of LSC Technology to Analysis of the Immune System in Health and Disease -- Concluding Remarks and Future Directions -- Acknowledgments -- References -- Chapter - 10 - Image Cytometry Analysis of Circulating Tumor Cells -- Abstract -- Introduction -- Cancer Metastasis -- Metastasis and Circulating Tumor Cells (CTCs) -- Preclinical Models of Metastasis and CTCs -- Background and Technical Considerations -- General Considerations -- Enrichment: Negative versus Positive Selection Approaches -- Choice of Tumor Markers for CTC Analysis -- Epithelial-Specific Markers -- Tumor-Associated Markers -- Techniques for CTC Enrichment and Isolation -- Cell Size and Density-Based Enrichment -- Enrichment by Immunomagnetic Separation -- Combination Approaches -- Techniques for CTC Detection and Analysis -- Nucleic Acid-Based Detection -- Cytometric Detection -- Image Cytometry: Methods and Results -- Laser Scanning Cytometry -- Methodology: Detection and Analysis of CTCs in Preclinical Models of Metastasis Using LSC (Allan et al., 2005 -- Goodale et al., 2009) -- Preclinical in vivo breast cancer metastasis models -- Labeling and immunomagnetic enrichment procedure -- Laser scanning cytometry analysis -- Typical Results: Detection and Analysis of CTCs in Preclinical Models of Metastasis Using LSC -- Practical Application of the Methodology: Detection and Analysis of CTCs in Preclinical Models of Metastasis Using LSC -- The CellSearch& -- reg -- System -- Methodology: Detection and Analysis of CTCs in Human Blood Samples Using the CellSearch& -- reg -- System -- Blood sample collection and preparation -- Detection and enumeration of CTCs using the CellSearch& -- reg -- system -- Molecular characterization of CTCs using the CellSearch& -- reg -- system. , Typical Results: Detection and analysis of CTCs in Human Blood Samples Using the CellSearch&.

Note: Front Cover -- Methods in Cell Biology -- CONTENTS -- Contents of Volume 64 -- Contributors -- Preface to the Third Edition -- Preface to the Second Edition -- Preface to the First Edition -- Part I: Principles of Cytometry and General Methods -- Chapter 1. A Brief History of Flow Cytometry and Sorting -- I. Introduction -- II. Instrumentation -- III. Applications -- References -- Chapter 2. Principles of Flow Cytometry: An Overview -- I. Introduction -- II. The Illumination of a Particle -- III. Fluidics: Centering Particles in the Illuminating Beam -- IV. Collection of Light Signals from Particles -- V. From Light Signals to a Data File -- VI. From Data to Information -- VII. Sorting -- VIII. Conclusions -- References -- Chapter 3. Laser Scanning Cytometry -- I. Introduction -- II. Background -- III. Description of the Instrument -- IV. The Utility and Operational Characteristics of Some Laser Scanning Cytometry List Mode Features -- V. Utility of Solid Phase Cytometry for Cell Preparation -- VI. Future Directions -- References -- Chapter 4. Principles of Confocal Microscopy -- I. Brief History of Microscope Development -- II. Development of Confocal Microscopy -- III. Image Formation in Confocal Microscopy -- IV. Useful Fluorescent Probes for Confocal Microscopy -- V. Applications of Confocal Microscopy -- VI. Conclusions -- References -- Chapter 5. Optical Measurements in Cytometry: Light Scattering, Extinction, Absorption, and Fluorescence -- I. Introduction -- II. Signal Processing Tasks in Flow Cytometry: An Overview -- III. The Optical Signal: Interaction of Light with Cells -- IV. Detection: Converting Optical Signals to Current -- V. Electronics: Converting Current to Voltage -- VI. Fluorescence Compensation and Logarithmic Amplification -- VII. Peak Detection, Integration, and Pulse Width Measurement. Triggering. , VIII. Measurement Sensitivity: Changing Concepts and the Bottom Line -- References -- Chapter 6. Flow Cytometric Fluorescence Lifetime Measurements -- I. Introduction -- II. Applications of the Technology -- III. Cell Preparation and Staining -- IV. Fluorescence Lifetime Flow Cytometry Instrumentation -- V. Results -- VI. Critical Aspects of the Technology -- VII. Future Directions -- References -- Chapter 7. Principles of Data Acquisition and Display -- I. Introduction -- II. Pulse Characterization Using Analog and Hybrid Circuits -- III. Analog Data Display -- IV. Analog-to-Digital Conversion -- V. Pulse Characterization by Digital Signal Processing -- VI. Data Storage and Display with Digital Computers -- References -- Chapter 8. Time as a Flow Cytometric Parameter -- I. Introduction -- II. Historical Overview -- III. Sample Mixing and Delivery -- IV. Data Analysis -- V. Applications -- VI. Conclusions -- References -- Chapter 9. Protein Labeling with Fluorescent Probes -- I. Introduction -- II. Labeling of Proteins with Organic Fluorescent Dyes -- III. Labeling of Proteins with Phycobiliproteins -- IV. Conclusion -- References -- Part II: Cell Preparation -- Chapter 10. Preparation of Cells from Blood -- I. Introduction -- II. Collection, Transport, and Storage of Blood -- III. Fixation and Preservation -- IV. Separation of Erythrocytes from Leukocytes -- V. Assessment of Cell Viability -- VI. Staining -- VII. Summary -- Appendix 1 -- Appendix 2 -- Appendix 3 -- References -- Chapter 11. Cell Preparation for the Identification of Leukocytes -- I. Introduction -- II. Antibodies -- III. Tandem Fluorochromes -- IV. Cell Preparation and Staining Procedures -- V. Titering Antibodies -- VI. Solutions and Reagents -- References -- Chapter 12. Strategies for Cell Permeabilization and Fixation in Detecting Surface and Intracellular Antigens. , I. Introduction -- II. Application -- III. Materials and Methods -- IV. Concluding Remarks -- References -- Part III: Standardization, Quality Assurance -- Chapter 13. Stoichiometry of Immunocytochemical Staining Reactions -- I. Introduction -- II. Structure of Immunoglobulin G -- III. Cell Structure -- IV. Permeabilized Cell Structure -- V. Antibody-Antigen Reactions -- VI. Multiparametric Analyses -- VII. Summary -- References -- Chapter 14. Standardization and Quantitation in Flow Cytometry -- I. Introduction -- II. General Issues -- III. Performance Characteristics-Dynamic Range, Linearity, Resolution, and Sensitivity -- IV. Standardization and Calibration of Common Cytometry Measurements -- V. Examples of Applications Using Calibrated Measurements -- VI. Issues in Quantitation of Fluorochromes and Other Molecules -- References -- Part IV: Cell Proliferation -- Chapter 15. Methods to Identify Mitotic Cells by Flow Cytometry -- I. Introduction -- II. Materials -- III. Cell Preparation and Staining -- IV. Instruments -- V. Critical Aspects of the Procedure -- VI. Results and Discussion -- VII. Comparison of Anti-H3-P Monoclonal Antibody with Other Markers of Mitotic Cells -- References -- Chapter 16. Cell Cycle Kinetics Estimated by Analysis of Bromodeoxyuridine Incorporation -- I. Introduction -- II. Applications -- III. Materials -- IV. Methods -- V. Critical Aspects of the Procedure -- References -- Chapter 17. Flow Cytometric Analysis of Cell Division History Using Dilution of Carboxyfluorescein Diacetate Succinimidyl Ester, a Stably Integrated Fluorescent Probe -- I. Introduction and Background -- II. Reagents and Solutions -- III. Preparation and Labeling of Cells -- IV. Gathering of Information Concurrent with Division -- V. Analysis of Data. , VI. Application of Carboxyfluorescein Diacetate Succinimidyl Ester to in Vitro Culture of Lymphocytes -- VII. Monitoring Lymphocyte Responses in Vivo -- VIII. Antigen Receptor Transgenic Models -- References -- Chapter 18. Antibodies against the Ki-67 Protein: Assessment of the Growth Fraction and Tools for Cell Cycle Analysis -- I. Introduction -- II. Application -- III. Materials and Methods -- IV. Critical Aspects -- V. Controls and Standards -- VI. Examples of Results -- References -- Chapter 19. Detection of Proliferating Cell Nuclear Antigen -- I. Introduction -- II. Molecular Biology of Proliferating Cell Nuclear Antigen -- III. Methods for Immunochemical Detection and Quantification of Proliferating Cell Nuclear Antigen -- IV. Results of Cytometric Analysis of Proliferating Cell Nuclear Antigen Expression -- V. Applications in Toxicology, Pathology, and Oncology -- References -- Chapter 20. Lymphocyte Activation Associated Antigens -- I. Introduction -- II. Methodological Aspects -- III. To Flow or Not to Flow for Assessing Lymphocyte Activation/ Proliferation? And, If Yes, How Reliable Is Immunophenotyping? -- IV. Additional Approaches -- V. Concluding Remarks -- References -- Part V: Cell Death/Apoptosis -- Chapter 21. Analysis of Mitochondria during Cell Death -- I. Introduction -- II. Scientific Background -- III. Apoptosis and Mitochondria -- IV. Method -- V. Results -- VI. Pitfalls and Misinterpretation of the Data -- VII. Comparison with Other Methods -- VIII. Reviews of the Applications -- IX. Biological and Biomedical Information -- X. Future Directions -- References -- Chapter 22. Cytometry of Caspases -- I. Introduction -- II. Materials and Methods: Caspase Peptide Inhibitors and Methods to Monitor Responses -- III. Results and Discussion -- References -- Chapter 23. Analysis of Apoptosis in Plant Cells -- I. Introduction. , II. Apoptosis in Plants -- III. Problems Associated with Analyzing Plant Cells Using Flow Cytometry -- IV. Morphological Changes of Plant Cells -- V. Physiological Changes during Apoptosis -- VI. Conclusion -- References -- Chapter 24. Difficulties and Pitfalls in Analysis of Apoptosis -- I. Introduction -- II. Apoptotic Index May Not Be Correlated with Incidence of Cell Death -- III. Dif.culties in Estimating Frequency of Apoptosis by Analysis of DNA Fragmentation -- IV. The Lack of Evidence Is Not Evidence for the Lack of Apoptosis -- V. Misclassification of Apoptotic Bodies or Nuclear Fragments as Single Apoptotic Cells -- VI. Apoptosis versus Necrosis versus ''Necrotic Stage'' of Apoptosis -- VII. Selective Loss of Apoptotic Cells during Sample Preparation -- VIII. Live Cells Engulfing Apoptotic Bodies Masquerade as Apoptotic Cells -- IX. The Problems with Commercial Kits and Reagents -- X. Cell Morphology Is Still the Gold Standard for Identification of Apoptotic Cells -- XI. Laser Scanning Cytometry: Have Your Cake and Eat It Too -- References -- Part VI: Cell-Cell, Cell-Environment Interactions -- Chapter 25. Analysis of Cell Migration -- I. Introduction and Application -- II. General Strategies to Measure Cell Migration -- References -- Chapter 26. Three-Dimensional Extracellular Matrix Substrates for Cell Culture -- I. Introduction -- II. Application -- III. Methods -- IV. Application of Intestinal Submucosa as a Three-Dimensional Extracellular Matrix Substrate -- V. Summary -- References -- Chapter 27. Three-Dimensional Imaging of Extracellular Matrix and Extracellular Matrix-Cell Interactions -- I. Introduction -- II. Three-Dimensional Imaging of Extracellular Matrix and Extracellular Matrix-Cell Interactions: Current Techniques and Their Limitations. , III. Three-Dimensional Microscopy of Living Systems: Extracellular Matrix and Extracellular Matrix-Cell Interactions.

Note: Front Cover -- Methods in Cell Biology: Flow Cytometry -- Copyright Page -- Contents -- Contributors -- Preface -- Chapter 1. Dissociation of Cells from Solid Tumors -- Chapter 2. Flow Cytometric Measurement of Cell Viability Using DNase Exclusion -- Chapter 3. Cell Cycle Phase-Specific Analysis of Cell Viability Using Hoechst 33342 and Propidium Iodide after Ethanol Preservation -- Chapter 4. Cell Membrane Potential Analysis -- Chapter 5. Flow Cytometric Measurement of Intracellular Ionized Calcium in Single Cells with Indo-l and Fluo-3 -- Chapter 6. Measurement of Intracellular pH -- Chapter 7. Flow Cytometric Techniques for Measurement of Cytochrome P-450 Activity in Viable Cells -- Chapter 8. Fluorescent Staining of Enzymes for Flow Cytometry -- Chapter 9. Supravital Cell Staining with Hoechst 33342 and DiOC5(3) -- Chapter 10. Specific Staining of DNA with the Fluorescent Antibiotics, Mithramycin, Chromomycin, and Olivomycin -- Chapter 11. DAPI Staining of Fixed Cells for High-Resolution Flow Cytometry of Nuclear DNA -- Chapter 12. High-Resolution DNA Measurements Using the Nuclear Isolation Medium, DAPI, with the RATCOM Flow Cytometer -- Chapter 13. Rapid DNA Content Analysis by the Propidium Iodide-Hypotonic Citrate Method -- Chapter 14. An Integrated Set of Methods for Routine Flow Cytometric DNA Analysis -- Chapter 15. DNA Analysis from Paraffin-Embedded Blocks -- Chapter 16. Detection of M and Early-Gl Phase Cells by Scattering Signals Combined with Identification of G1, S, and G2 Phase Cells -- Chapter 17. Controls, Standards -- and Histogram Interpretation in DNA Flow Cytometry -- Chapter 18. Cell Division Analysis Using Bromodeoxyuridine-Induced Suppression of Hoechst 33258 Fluorescence -- Chapter 19. Cell Cycle Analysis Using Continuous Bromodeoxyuridine Labeling and Hoechst 33258-Ethidium Bromide Bivariate Flow Cytometry. , Chapter 20. Detection of Bromodeoxyuridine-Labeled Cells by Diffeential Fluorescence Analysis of DNA Fluorochromes -- Chapter 21. Using Monoclonal Antibodies in Bromodeoxyuridine-DNA Analysis -- Chapter 22. Identification of Proliferating Cells by Ki-67 Antibody -- Chapter 23. Washless Double Staining of a Nuclear Antigen (Ki-67 or Bromodeoxyuridine) and DNA in Unfixed Nuclei -- Chapter 24. Analysis of Proliferation-Associated Antigens -- Chapter 25. The Stathmokinetic Experiment: A Single-Parameter and Multiparameter Flow Cytometric Analysis -- Chapter 26. Detection of Intracellular Virus and Viral Products -- Chapter 27. Differential Staining of DNA and RNA in Intact Cells and Isolated Cell Nuclei with Acridine Orange -- Chapter 28. Flow Cytometric Analysis of Double-Stranded RNA Content Distributions -- Chapter 29. Simultaneous Fluorescent Labeling of DNA, RNA, and Protein -- Chapter 30. Flow Cytometric Cell-Kinetic Analysis by Simultaneously Staining Nuclei with Propidium Iodide and Fluorescein Isothiocyanate -- Chapter 31. Flow Cytometric Methods for Studying Isolated Nuclei: DNA Accessibility to DNase I and Protein-DNA Content -- Chapter 32. Acid-Induced Denaturation of DNA in Situ as a Probe of Chromatin Structure -- Chapter 33. Fluorescent Methods for Studying Subnuclear Particles -- Chapter 34. Chromosome and Nuclei Isolation with the MgSO4 Procedure -- Chapter 35. Univariate Analysis of Metaphase Chromosomes Using the Hypotonic Potassium Chloride-Propidium Iodide Protocol -- Chapter 36. Polyamine Buffer for Bivariate Human Flow Cytogenetic Analysis and Sorting -- Chapter 37. Fluorescence in Situ Hybridization with DNA Probes -- Chapter 38. Flow Cytometric Analysis of Male Germ Cell Quality -- Chapter 39. Cell Preparation for the Identification of Leukocytes -- Chapter 40. Multiparameter Analysis of Leukocytes by Flow Cytometry. , Chapter 41. Identification and Purification of Murine Hematopoietic Stem Cells by Flow Cytometry -- Chapter 42. Fluorescent Cell Labeling for in Vivo and in Vitro Cell Tracking -- Chapter 43. Rapid Determination of Cellular Resistance-Related Drug Efflux in Tumor Cells -- Chapter 44. Slit-Scan Flow Analysis of Cytologic Specimens from the Female Genital Tract -- Chapter 45. Cell Sorting with Hoechst or Carbocyanine Dyes as Perfusion Probes in Spheroids and Tumors -- Chapter 46. DNA Measurements of Bacteria -- Chapter 47. Isolation and Flow Cytometric Characterization of Plant Protoplasts -- Chapter 48. Flow Cytometric Analysis of Plant Genomes -- Chapter 49. Plant Cell Cycle Analysis with Isolated Nuclei -- Chapter 50. Environmental Health: Flow Cytometric Methods to Assess Our Water World -- Chapter 51. Flow Microsphere Immunoassay for the Quantitative and Simultaneous Detection of Multiple Soluble Analytes -- Chapter 52. On-Line Flow Cytometry: A Versatile Method for Kinetic Measurements -- Chapter 53. Calibration of Flow Cytometer Detector Systems -- Chapter 54. Spectral Properties of Fluorochromes Used in Flow Cytometry -- Index -- Contents of Recent Volumes.

Note: Cover -- Methods in Cell Biology Volume 103 -- Copyright -- Contents -- Contributors -- Preface To Fifth Edition -- Section - 1 - New applications in cell biology -- Chapter - 1 - Recent Advances in Cytometry Applications: Preclinical, Clinical, andCellBiology -- Abstract -- Preclinical and Clinical Applications -- Cell Biology and Cell Transplantation Therapy -- Acknowledgment -- References -- Chapter - 2 - Detection of Hematopoietic Stem Cells by Flow Cytometry -- Abstract -- Introduction -- The side population is highly enriched in hematopoietic stem cells -- Surface markers for LT-HSC purification -- References -- Chapter - 3 - Identification of Very Small Embryonic/Epiblast-Like Stem Cells (VSELs) Circulating in Peripheral Blood During Organ/Tissue Injuries -- Abstract -- Introduction -- Background -- Very Small Embryonic-Like Stem Cells (VSELs) -- Developmental Origin of VSELs -- VSELs and Their Unique Molecular Characteristics -- Epigenetic Changes of Imprinted Genes that Regulate VSELs Pluripotency -- Mesenchymal Stem Cells (MSCs) -- Endothelial Progenitor Cells (EPCs) -- Materials -- Preparation of Peripheral Blood (PB) for Analysis -- Staining of Total PB-Derived Nucleated Cells (TNCs) for Analysis -- Methods -- Isolation of Total PB-Derived Nucleated Cells (TNCs) by Lysing Red Blood Cells (RBCs) -- Staining of PB-Derived TNCs for Flow Cytometric Analysis -- Setting Up Instrument for FACS Analysis -- Identification of VSELs in Human PB and UCB -- Calculation of Absolute Numbers of Target Cells in 1& -- mu -- L of PB -- Sorting of Cells -- Results -- Critical Aspects of the Methodology -- Applications -- Future Directions -- Acknowledgments -- References -- Chapter - 4 - Apoptosis and Beyond: Cytometry in Studies of Programmed Cell Death -- Abstract -- Introduction -- The Biology of Apoptosis -- Cytometry in Cell Necrobiology. , Cytometric Methods to Detect Apoptosis -- Light Scattering Changes in Apoptotic Cells -- Dissipation of Mitochondrial Transmembrane Potential (& -- Delta -- & -- psi -- m) -- Activation of Caspases -- Fluorochrome-Labeled Inhibitors of Caspases (FLICA) -- Detection of PARP Cleavage -- Changes in the Plasma Membrane During Apoptosis -- Externalization of Phosphatidylserine -- Changes in Plasma Membrane Permeability -- Nuclear Hallmarks of Apoptosis -- Assessment of Fractional DNA Content (Sub-G1 Fraction) -- Assessment of DNA Strand Breaks (TUNEL Assay) -- SYTO-Based Detection of Apoptosis -- Time-Window in Measuring Incidence of Apoptosis -- Multiparameter Detection of Apoptosis: Choosing the Right Method -- Beyond Apoptosis & -- ndash -- Analysis of Alternative Cell Death Modes -- Autophagy -- Necrosis -- Cell Senescence -- Future Outlook -- Acknowledgements -- References -- Chapter - 5 - Assessment of Oxidative Stress-Induced DNA Damage by Immunoflourescent Analysis of 8-OxodG -- Abstract -- Redox Regulation of Cell Fate Signaling -- 8-OxodG as a Marker of Oxidative DNA Damage -- 8-OxodG is a Highly Mutagenic DNA Lesion -- 8-OxodG as a Biomarker of Cellular Oxidative Damage and its Clinical Relevance -- Detection of Oxidative DNA Damage Involving 8-OxodG -- Chromatography-Based Direct Assessment of 8-OxodG -- The Indirect Assessment of OxodG Modification of DNA -- Indirect Assessment of Oxidative DNA Damage by Immunofluorescence -- Materials -- Methodology -- Results -- Shortcomings of the Immunofluorescence Assay -- Concluding Remarks -- References -- Chapter - 6 - Analysis of Individual Molecular Events ofDNA Damage Response by Flow- andImage-Assisted Cytometry -- Abstract -- Introduction -- Events of the DDR -- Chromatin Decondensation (Relaxation) -- Activation of Phosphatidyl Inositol 3& -- prime. , Kinase-Related Kinases (PIKKs) -- Activation of Checkpoint Kinases -- Histone H2AX Phosphorylation -- Detection of DDR Events by Cytometry -- Chromatin Relaxation (Decondensation) -- Recruitment of Mre11 -- Immunocytochemical Detection of DDR-Associated ATM, DNA-PKcs and Chk2 Activation, Phosphorylation of p53 and Histone H2AX -- Application of Cytometry to Detect DDR Induced byDifferent Genotoxic Agents -- Assessment of DDR Induced by DNA Topoisomerase Inhibitors -- Induction of DDR by Ionizing Radiation and UV Light -- Induction of DDR by Cigarette Smoke (CS) and Other Environmental Mutagens -- Oxidative DNA Damage -- Interpretation of Cytometric Data: Role of Image-Assisted Cytometry -- Role of Microfluidic Lab-on-a-Chip Platforms for DDR Analysis -- References -- Chapter - 7 - Fluorescence-Based Detection and Quantification of Features of Cellular Senescence -- Abstract -- Introduction -- Features Associated with Loss of Reproductive Cell Capability -- Shortened Telomeres in Replicative Senescence -- Telomere Dysfunction-Induced Foci (TIF) in Replicative Senescence -- Loss of the Doubling Capacity of Cells -- Cellular Hypertrophy -- Changes Associated with Lysosomes -- Lipofuscin Accumulation -- Lipofuscin Autofluorescence in Fluorescence Microscopy -- Flow Cytometric Analysis of Cellular Lipofuscin Levels -- Increased Lysosome Content -- Senescence-Associated & -- beta -- -Galactosidase (SA & -- beta -- -Gal) Activity -- In situ Determination of SA & -- beta -- -Gal Activity -- Flow Cytometry for SA & -- beta -- -Gal-Positive Cells and Quantification of Activity -- Changes Associated with Mitochondria -- Increased Mitochondrial Mass -- Quantification of Changes in Mitochondria Mass -- Altered Structural Dynamics of Mitochondria -- Visualization of Altered Mitochondria Structure -- Decreased Membrane Potential. , Quantitative Comparison of MMP -- Decreased Autophagy -- Determination of Autophagy Activity -- Changes in the Level of Reactive Oxygen Species (ROS) -- Changes in Level of Superoxide -- Changes in Level of Hydroxyl Radicals -- Changes Associated with Nucleus and Chromosomes -- Use of Flow Cytometry for Analysis of Cellular Senescence -- Conclusion -- Acknowledgments -- References -- Chapter - 8 - Measurement of Telomere Length UsingPNA Probe by Cytometry -- Abstract -- Introduction to Telomeres -- Technical Background -- Methods -- Our Protocol Versus the Original Flow-FISH Protocol -- Our Protocol -- Original Flow-FISH Protocol -- Results -- Critical Aspects of the Flow-FISH Methodology -- Conclusion and Perspectives -- References -- Section - 2 - Pre-clinical and clinical applications -- Chapter - 9 - Cytometry of Intracellular Signaling: From Laboratory Bench to Clinical Application -- Abstract -- Introduction -- Activation States Are Transient -- Most Signaling Pathways Exist in Their Ground State -- Constitutive Activation of Signaling Pathways Can Occur in Leukemia -- Rationale -- Methods -- Protocol for Whole Blood Fixation and Red Cell Lysis -- Samples -- Sample Suitability -- Addition of Pathway Activators or Inhibitors -- Whole Blood Fixation -- Red Cell Lysis -- Washing and Alcohol Treatment -- Antibody Staining -- Activators and Inhibitors of Signaling Pathways -- Pathway Activators -- Pathway Inhibitors -- Freezing Samples -- Materials -- Fixation and Red Cell Lysis -- Pathway Activators -- Pathway Inhibitors -- Antibodies -- Discussion -- Summary -- References -- Chapter - 10 - Immunophenotypic Pattern of Myeloid Populations by Flow Cytometry Analysis -- Abstract -- Introduction -- Granulocytes -- Basophils -- Eosinophils -- Monocytes -- Erythroid Precursors -- Megakaryocytes. , Acute Myeloid Leukemia (AML) with Minimal Differentiation and AML without Maturation -- AML with Maturation -- AML with t(8 -- 21) -- Acute Promyelocytic Leukemia (APL) -- Acute Myelomonocytic Leukemia (AML-M4) -- Acute Monoblastic Leukemia -- Acute Erythroid Leukemia (FAB: AML-M6) -- Acute Megakaryoblastic Leukemia (FAB: AML-M7) -- Blastic Plasmacytoid Dendritic Cell Neoplasm -- Chronic Myeloid Leukemia (CML, BCR& -- ndash -- ABL+) -- Granulocytes/Maturing Myeloid Precursors with Dyspoietic Features Associated with Myelodysplastic Syndrome (MDS) -- Chronic Myelomonocytic Leukemia (CMML) -- References -- Chapter - 11 - Flow Cytometry-Based Pharmacodynamic Monitoring After Organ Transplantation -- Abstract -- Introduction -- T Cells -- Regulatory T Cells -- Dendritic Cells -- Conclusions -- References -- Chapter - 12 - Clinical Cytometry and Progress in HLA Antibody Detection -- Abstract -- Introduction -- Cell-Based Assays -- Lymphocytotoxicity -- Modified Lymphocytotoxicity Assays -- Flow Cytometry -- Antigen-Based Assays -- Solid-Phase Immunobinding Assays -- Multiplex Platform for Microparticle Analysis -- Recombinant HLA Antigens -- Determining Antibody Specificity -- Current Frontiers in Transplant-Related Antibody Testing -- Extended HLA Loci -- Conundrums in HLA Antibody Assignment -- Functional Assessments of HLA Alloantibody by Flow Cytometry -- Summary -- References -- Chapter - 13 - Clinical Utility of Flow Cytometry intheStudy of Erythropoiesis and Nonclonal Red Cell Disorders -- Abstract -- Keywords -- Introduction -- Normal Erythroid Development -- Flow Cytometry for Reticulocyte Enumeration -- Flow Cytometry for the Detection of Fetal& -- ndash -- Maternal Hemorrhage -- Flow Cytometry for Quantitation of HbF in Sickle Cell Disease -- Flow Cytometry in the Evaluation of Hereditary Spherocytosis. , Measurement of Red Cell Survival and Red Cell Volume.

Note: Front Cover -- Methods in Cell Biology: Flow Cytometry -- Copyright Page -- Content -- Contents of Volume 41 -- Contributors -- Preface to the Second Edition -- Preface to the First Edition -- Chapter 1. A Flow Cytometric Technique for Detection of DNA Repair in Mammalian Cells -- I. Introduction -- II. Application -- III. Materials -- IV. Sample Preparation and Staining -- V. Critical Aspects of the Procedure -- VI. Controls and Standards -- VII. Instruments and Setup for Data Collection -- VIII. Results and Discussion -- References -- Chapter 2. Rapid Determination of Cellular Resistance-Related Drug Efflux in Tumor Cells -- I. Introduction -- II. Application -- III. Materials -- IV. Staining Procedure -- V. Critical Aspects -- VI. Controls and Standards -- VII. Instruments -- VIII. Results -- References -- Chapter 3. Glutathione and Cellular Resistance to Anti-Cancer Drugs -- I. Introduction -- II. Flow Cytometric Glutathione Probes -- Ill. Materials -- IV. Methods -- References -- Chapter 4. Assay of Cell Resistance to Ara-C -- I. Introduction -- II. Application -- III. Materials -- IV. Cell Preparation and Staining -- V. Critical Aspects of the Procedure -- VI. Instruments -- VII. Results and Discussion -- VIII. Comparison of the Methods -- References -- Chapter 5. Detection of mRNA Species by Flow Cytometry -- I. Introduction -- II. Applications -- III. Materials -- IV. Staining Procedures -- V. Critical Aspects of the Procedure and Controls -- VI. Instruments and FCM Analysis -- VII. Results and Discussion -- References -- Chapter 6. Primed in Situ Labeling (PRINS) and Fluorescence in Situ Hybridization (FISH) -- I. Introduction -- II. Applications -- III. Materials -- IV. Protocols -- V. Critical Aspects of the Procedures -- VI. Instruments -- VII. Results and Discussion -- VIII. Comparison of Methods -- References. , Chapter 7. Molecular Phenotyping by Flow Cytometry -- I. Introduction -- II. General Materials and Methods -- III. Measurement of Gene Amplification Using Competitive PCR -- IV. Measurement of Gene Expression Using Competitive RT-PCR -- V. Fluorescence in Situ Hybridization En Suspension (FISHES) -- VI. Fluorescence in Situ PCR (FLIP) -- References -- Chapter 8. Isolation and Analysis of Somatic Cell Mutants with Defects in Endocytic Traffic -- I. Introduction: Why Isolate Somatic Cell Mutants in Membrane Traffic? -- II. General Considerations -- III. Isolation of CHO Cells Defective in Receptor Recycling -- IV. Isolation of B Cells with Reduced Cysteine Protease Activity -- V. Conclusions -- References -- Chapter 9. Measurement of Micronuclei by Flow Cytometry -- I. Introduction -- II. Application -- III. Materials -- IV. Cell Preparation and Staining -- V. Critical Aspects of the Procedure -- VI. Standards -- VII. Instruments -- VIII. Results and Discussion -- References -- Chapter 10. Sperm Chromatin Structure Assay: DNA Denaturability -- I. Introduction -- II. Applications of the SCSA -- III. Materials -- IV. Cell Preparation -- V. Cell Staining and Measurement -- VI. Instruments -- VII. Results and Discussion -- VIII. Critical Points -- References -- Chapter 11. Fine-Needle Cytopuncture and Flow Cytometry in the Characterization of Human Tumors -- I. Introduction -- II. Materials and Methods -- III. Critical Aspects -- IV. Alternative Methods Applied to Cytopunctures -- V. Clinical Applications -- VI. Conclusion -- References -- Note Added in Proof -- Chapter 12. Functional N K Assays Using Flow Cytometry -- I. Introduction -- II. Application -- III. Materials -- IV. Cell Preparation and Staining -- V. Critical Aspects of the Procedures -- VI. Standardization -- VII. Instruments -- VIII. Results and Discussion -- References. , Chapter 13. Antibodies to Intermediate Filament Proteins as Probes for Multiparameter Flow Cytometry of Human Cancers -- I. Introduction -- II. Intermediate Filament Proteins -- III. Cell Isolation and Fixation -- IV. Multiparameter Flow Cytometric Analysis Using Antibodies to Cytokeratins -- V. Clinical Application Using Multiparameter DNA Content Flow Cytornetry -- VI. Conclusions -- References -- Chapter 14. Interactive Data Analysis for Evaluation of B-Cell Neoplasia by Flow Cytometry -- I. Background -- II. Correlated Multiparametric Data Analysis of B-Cell Neoplasia -- References -- Chapter 15. Analysis and Sorting of Hematopoietic Stem Cells from Mouse Bone Marrow -- I. Introduction -- II. Application -- III. Materials -- IV. Instruments -- V. Staining and Sorting Procedure -- VI. Critical Aspects of the Procedure -- VII. Results and Discussion -- References -- Chapter 16. Reticulocyte Analysis and Reticulocyte Maturity Index -- I. Introduction -- II. Reticulocyte Maturity Index -- III. Data Analysis -- IV. Quality Control -- V. Method for Thiazole Orange Reticulocyte Analysis -- References -- Chapter 17. Analysis of Platelets by Flow Cytometry -- I. Introduction -- II. Applications -- III. Materials -- IV. Cell Preparation -- V. Staining -- VI. Critical Aspects -- VII. Standards -- VIII. Instrument -- IX. Results and Discussion -- References -- Chapter 18. Flow Cytometry in Malaria Detection -- I. Introduction -- II. Applications -- III. Materials -- IV. Cell Preparation and Staining -- V. Critical Aspects of the Preparation and Staining Procedures -- VI. Standards -- VII. Instruments -- VIII. Results and Discussion -- IX. Comparison of Methods -- References -- Chapter 19. Large-Scale Chromosome Sorting -- I. Introduction -- II. Sample Preparation -- III. Preparation Evaluation -- IV. Production Chromosome Sorting. , Appendix: Polyamine Sheath Buffer -- References -- Chapter 20. Strategies for Rare Cell Detection and Isolation -- I. Introduction -- II. Methods -- III. Critical Aspects of the Procedure -- IV. Instrumentation -- V. Results: Some Rare Cell Applications and Strategies -- VI. Discussion -- References -- Chapter 21. Cell Sorting of Biohazardous Specimens for Assay of Immune Function -- I. Introduction -- II. Applications -- III. Critical Aspects of the Procedure -- IV. Instruments -- V. Verification of Aerosol Containment -- VI. Staining and Cell Separation of Potentially Biohazardous Specimens -- VII. Results and Discussion -- VIII. Alternate Technologies for Cell Separation -- References -- Chapter 22. High-Speed Photodamage Cell Sorting: An Evaluation of the ZAPPER Prototype -- I. Introduction -- II. Application -- III. Materials -- IV. Critical Aspects of the Procedure -- V. Instrumentation -- VI. Results and Discussion -- References -- Chapter 23. High-Gradient Magnetic Cell Sorting -- I. Introduction -- II. Application -- III. Materials -- IV. MACS: Staining and Sorting -- V. Critical Aspects of the Procedure -- VI. Controls -- VII. Instruments -- VIII. Results -- References -- Chapter 24. Contributions of Flow Cytometry to Studies with Multicell Spheroids -- I. Introduction -- II. Spheroid Growth and Analysis -- III. Flow Cytonietry Techniques -- IV. Studies of Spheroid Oxygenation -- V. Evaluation of Antineoplastic Drugs -- VI. Drug Interactions: Synergy in Cell Subpopulations -- VII. Multifraction Treatments -- VIII. Cell Kinetics: Role of Pretreatment Kinetics -- IX. Alternatives for Kinetic Measurements: Overcoming Heterogeneity -- X. General Conclusions -- References -- Chapter 25. Functional Measurements Using HL-60 Cells -- I. Introduction -- II. Application -- III. Materials and Methods -- IV. Results and Discussion -- References. , Chapter 26. HIV Infection: Diagnosis and Disease Progression Evaluation -- I. Introduction -- II. Application -- III. Materials -- IV. Cell Preparation and Staining -- V. Critical Aspects of the Procedure -- VI. Controls and Standards -- VII. Instruments -- VIII. Results and Discussion -- IX. Alternative Technologies -- References -- Chapter 27. Cell-Cycle Analysis of Saccharomyces cerevisiae -- I. Introduction -- II. Materials -- III. Methods -- IV. Critical Aspects -- V. Flow Cytometry -- VI. Analysis of DNA Histograms -- VII. Results -- References -- Chapter 28. Staining and Measurement of DNA in Bacteria -- I. Introduction -- II. Materials -- III. Fixation and Staining -- IV. Vital Staining -- V. Flow Cytometric Measurement -- VI. Standards and Controls -- References -- Chapter 29. Detection of Specific Microorganisms in Environmental Samples Using Flow Cytometry -- I. Introduction -- II. Preparation of Water Samples for Flow Cytometric Analysis -- III. Staining of Organisms from Water Samples for Flow Cytometric Analysis -- IV. Flow Cytometric Analysis of Water Samples -- V. Instrumentation Developments for Environmental Applications -- VI. The Future of Flow Cytometry within Environmental Microbiology -- References -- Chapter 30. Strategies for Flow Cytometric Analysis of Marine Microalgae and Sponge Cells -- I. Introduction -- II. Application -- III. Materials -- IV. Cell Preparation and Staining -- V. Instrumentation -- VI. Results -- VII. Discussion -- References -- Chapter 31. Flow Cytometry and Sorting of Plant Protoplasts and Cells -- I. Introduction -- II. Application -- III. Materials -- IV. Procedures -- V. Critical Aspects of the Procedures -- VI. Controls and Standards -- VII. Instruments -- VIII. Results -- References. , Chapter 32. Use of Fluorescence-Activated Cell Sorting for Rapid Isolation of Insect Cells Harboring Recombinant Baculovirus.

Note: Front Cover -- Cytometry, 4th Edition: New Developments -- Copyright Page -- Contents -- Contributors -- Preface -- Preface to the Third Edition -- Preface to the Second Edition -- Preface to the First Edition -- Part I: Instrumentation/Fluorochromes -- Chapter 1. Optimization of Emission Optics for Multicolor Flow Cytometry -- I. Introduction -- II. Fluorochromes -- III. Hardware Components Affecting Light Collection -- IV. Maximizing Signal Intensities -- V. Maximizing Signal Collection Quality -- VI. Emission Collection Optimization -- VII. Testing Emission Optics -- References -- Chapter 2. Two-Photon Tissue Cytometry -- I. Introduction -- II. Technical Development of Two-Photon Tissue Cytometry -- III. Experimental Demonstration of Two-Photon Tissue Cytometry -- IV. Future Outlook -- V. Conclusion -- References -- Chapter 3. Confocal Microscopy: Quantitative Analytical Capabilities -- I. Introduction -- II. Cartesian Coordinates, Distance, Size, Shape, and Volume in Confocal Microscopy -- III. Measurements of Fluorescence Intensity in Confocal Microscopy -- IV. Time Resolution in Confocal Microscopy -- V. Confocal Techniques Based on Measurements of Fluorescence Intensity vs Time -- VI. Spectrally Resolved Confocal Microscopy -- VII. Conclusion -- References -- Chapter 4. Surface-Plasmon-Coupled Emission. New Technology for Studying Molecular Processes -- I. Introduction -- II. Overview of Surface Plasmon Resonance -- III. Surface-Plasmon-Coupled Emission -- IV. Experimental Studies of Surface-Plasmon-Coupled Emission -- V. Practical Applications -- VI. Conclusion -- References -- Chapter 5. Cytometry of Fluorescence Resonance Energy Transfer -- I. Introduction -- II. Theory of FRET -- III. Measuring FRET -- IV. Applications -- V. Perspectives -- VI. Conclusions -- References -- Chapter 6. The Rainbow of Fluorescent Proteins. , I. The Fluorescent Proteins -- II. Flow Analysis Using Fluorescent Proteins -- III. Flow Sorting Using Fluorescent Proteins -- IV. Conclusion -- References -- Chapter 7. Labeling Cellular Targets with Semiconductor Quantum Dot Conjugates -- I. Introduction -- II. Selection of QDs and Their Conjugates -- III. Labeling of Fixed Cells for Fluorescence Microscopy -- IV. Measurements on Living Cells Using QD Conjugates -- V. Conclusion -- References -- Chapter 8. Next-Generation Laser Scanning Cytometry -- I. Introduction -- II. LSC-Hybrid Technology -- III. Description of the LSC Platform -- IV. Selected Applications -- V. Utility of Solid-Phase Imaging Cytometry in Life Science Research and Drug Discovery -- References -- Part II: General Techniques -- Chapter 9. Biohazard Sorting -- I. Introduction -- II. Critical Aspects of the Procedure -- III. Applications and Future Directions -- References -- Chapter 10. Guidelines for the Presentation of Flow Cytometric Data -- I. Introduction -- II. General Principles of Graphical Presentation -- III. Statistics -- IV. Subset Analysis -- V. Conclusion -- References -- Chapter 11. Mechanism of Antitumor Drug Action Assessed by Cytometry -- I. Introduction -- II. Choice of a Model System -- III. Drug Dose and Length of Exposure -- IV. Single-Parameter DNA Histogram Analysis -- V. Multiparameter Approaches -- VI. Data Presentation -- VII. Summary and Future Directions -- References -- Chapter 12. Cytometric Methods to Detect Apoptosis -- I. Introduction -- II. Physical and Molecular Features of Cells Dying by Apoptosis or Necrosis -- III. Different Time-Windows for Detection of Apoptosis: Possible Source of Error in Measurement of Incidence of Apoptosis -- IV. Light-Scattering Properties of Cells Dying by Apoptosis -- V. Mitochondrial Transmembrane Potential -- VI. Activation of Caspases -- VII. Annexin V Binding. , VIII. DNA Fragmentation -- IX. Susceptibility of DNA to Denaturation -- X. Activation of ''Tissue'' Transglutaminase -- XI. Measuring Incidence of Apoptosis: Which Method to Choose? -- References -- Chapter 13. Real-Time Analysis of Apoptosis In Vivo -- I. Introduction -- II. In Vivo Membrane Markers -- III. A New Generation of Caspase Markers -- IV. Conclusion -- References -- Chapter 14. Detection of DNA Damage in Individual Cells by Analysis of Histone H2AX Phosphorylation -- I. Introduction -- II. Considerations in the Use of yH2AX as a Measure of Double-Stranded Breaks -- III. Methods of Analysis -- IV. Typical Results -- V. Possible Applications -- VI. Conclusion -- References -- Chapter 15. Cytometry of Freshwater Phytoplankton -- I. Introduction -- II. Background -- III. Methods -- IV. Results -- V. Limitations and Applications -- Future Directions -- References -- Chapter 16. Multiplexed Microsphere Assays for Protein and DNA Binding Reactions -- I. Introduction -- II. Technology and Instrumentation -- III. Methods -- IV. Results -- V. Software -- VI. Critical Aspects of the Methodology -- VII. Comparison with Other Methods -- VIII. Future Directions -- References -- Part III: Immunology/T-Cell Responses -- Chapter 17. Flow Cytometry Applications of MHC Tetramers -- I. Introduction -- II. Materials and Methods -- III. Analyzing Tetramer Data -- IV. Combining Tetramer Staining with Functional Assays -- V. Conclusion -- References -- Chapter 18. Use of Peptides and Peptide Libraries as T-Cell Stimulants in Flow Cytometric Studies -- I. Introduction -- II. Background -- III. Results/Discussion of the Literature -- IV. Technical Considerations -- V. Specific Applications -- VI. Potential Clinical Use -- VII. Protocols -- References -- Chapter 19. Flow Cytometric Analysis of Human Antigen-Specific T-Cell Proliferation. , I. Introduction and Background -- II. Methods -- III. Results -- IV. Critical Aspects of the Methodology -- V. Pitfalls and Misinterpretation of the Data -- VI. Future Directions -- References -- Chapter 20. Detection of T-Cell Degranulation: CD107a and b -- I. Introduction: Methods to Assess CD8+ T-Cell Function -- II. Materials and Methods -- III. Results -- IV. Critical Aspects -- V. Applications -- References -- Chapter 21. T-Cell Responses to Cancer -- I. Introduction -- II. Methods to Identify and Enumerate TAA-Specific T Cells -- III. Important Considerations -- IV. Methods to Further Analyze In Vivo Biology of Antitumor T Cells -- V. Novel Issues and Methods -- VI. Conclusion -- References -- Part IV: Multi-Color Immunophenotyping -- Chapter 22. Multicolor Flow Cytometric Analysis in SIV-Infected Rhesus Macaque -- I. Introduction -- II. Methods -- III. Flow Cytometry Instrumentation and Software -- IV. Instrument Setup -- V. Critical Aspects of the Technology -- VI. Application of 10-Color Analysis to SIV Infection -- VII. Conclusion -- References -- Chapter 23. Multicolor Immunophenotyping: Human Immune System Hematopoiesis -- I. Introduction -- II. Methodology -- III. Normal Immunophenotypic Patterns of Maturation -- IV. Abnormal Immunophenotypic Patterns of Maturation -- References -- Chapter 24. Multicolor Immunophenotyping: Human Mature Immune System -- I. Introduction -- II. Choice of Markers -- III. Staining Panels: Matching Fluorochrome with Cell Marker -- IV. Conclusion -- References -- Chapter 25. Differential Diagnosis of T-Cell Lymphoproliferative Disorders by Flow Cytometry Multicolor Immunophenotyping. Correlation with Morphology -- I. Introduction -- II. Materials -- III. Methods -- IV. Identification of Abnormal T-Cell Population by Flow Cytometry -- V. Precursor T Lymphoblastic Lymphoma/Leukemia. , VI. Peripheral (Mature/Post-thymic) Lymphoma Versus Precursor T-Lymphoblastic Lymphoma/Leukemia -- VII. Thymocytes from Thymic Hyperplasia/Thymoma Versus Precursor T-Lymphoblastic Lymphoma/Leukemia -- VIII. Mature T-Cell Lymphoproliferative Disorders -- IX. CD117 Expression in T-Cell Lymphoproliferations -- X. Blastic NK Cell Lymphoma (DC-2 Acute Leukemia) -- XI. Conclusion -- References -- Chapter 26. Isolation and Immunophenotyping of Human and Rhesus Macaque Dendritic Cells -- I. Introduction -- II. In Vitro Differentiation of Monocyte-Derived Dendritic Cells -- III. Detection and Sorting Procedures of Subsets of Dendritic Cells -- IV. Culture and Activation of Dendritic Cells -- References -- Chapter 27. B-Cell Immunophenotyping -- I. Introduction -- II. Immunophenotyping of B-Cell Developmental Stages in the Bone Marrow -- III. Peripheral B-Cell Populations -- IV. Antigen-Induced B-Cell Subsets -- V. Antigen-Specific B Cells -- References -- Part V: Other Topics in Immunology -- Chapter 28. Flow Cytometry Immunophenotypic Characteristics of Monocytic Population in Acute Monocytic Leukemia (AML-M5), Acute Myelomonocytic Leukemia (AML-M4), and Chronic Myelomonocytic Leukemia (CMML) -- I. Introduction -- II. Materials -- III. Methods -- IV. Acute Monocytic Leukemia -- V. Chronic Myelomonocytic Leukemia -- VI. Acute Myelomonocytic Leukemia -- VII. Differential Diagnosis -- VIII. Conclusion -- References -- Chapter 29. Phagocyte Function -- I. Introduction -- II. Background -- III. Methods -- IV. Results -- V. Pitfalls and Misinterpretation of the Data -- VI. Comparison with Other Methods -- VII. Applications and Biomedical Information -- VIII. Future Directions -- References -- Chapter 30. Neutralizing Antibody Quantification by Flow Cytometry -- I. Introduction -- II. Background -- III. Results -- IV. Discussion and Key Points -- References. , Part VI: Cytogenetics.

Note: Front Cover -- Essential Cytometry Methods -- Copyright Page -- Contents -- Contributors -- Preface -- Part I: Fluorochromes/General Techniques -- Chapter 1: Principles of Confocal Microscopy -- I. Brief History of Microscope Development -- II. Development of Confocal Microscopy -- III. Image Formation in Confocal Microscopy -- IV. Useful Fluorescent Probes for Confocal Microscopy -- V. Applications of Confocal Microscopy -- VI. Conclusions -- References -- Chapter 2: Protein Labeling with Fluorescent Probes -- I. Update -- II. Introduction -- III. Labeling of Proteins with Organic Fluorescent Dyes -- IV. Labeling of Antibodies with Zenon Probes -- V. Labeling of Proteins with Phycobiliproteins -- VI. Conclusion -- References -- Chapter 3: Cytometry of Fluorescence Resonance Energy Transfer -- I. Introduction -- II. Theory of FRET -- III. Measuring FRET -- IV. Applications -- V. Perspectives -- VI. Conclusions -- Acknowledgments -- References -- Chapter 4: The Rainbow of Fluorescent Proteins -- I. Update -- II. The Fluorescent Proteins -- III. Flow Analysis Using Fluorescent Proteins -- IV. Flow Sorting Using Fluorescent Proteins -- V. Conclusion -- Acknowledgments -- References -- Chapter 5: Labeling Cellular Targets with Semiconductor Quantum Dot Conjugates -- I. Introduction -- II. Selection of QDs and their Conjugates -- III. Labeling of Fixed Cells for Fluorescence Microscopy -- IV. Measurements in Living Cells Using QD Conjugates -- V. Conclusion -- Acknowledgment -- References -- Chapter 6: High-Gradient Magnetic Cell Sorting -- I. Update -- II. Introduction -- III. Application -- IV. Materials -- V. MACS: Staining and Sorting -- VI. Critical Aspects of the Procedure -- VII. Controls -- VIII. Instruments -- IX. Results -- Acknowledgment -- References -- Chapter 7: Multiplexed Microsphere Assays (MMAs) for Protein and DNA Binding Reactions. , I. Update -- II. Introduction -- III. Technology and Instrumentation -- IV. Methods -- V. Results -- VI. Software -- VII. Critical Aspects of the Methodology -- VIII. Comparison with Other Methods -- IX. Future Directions -- Acknowledgment -- References -- Chapter 8: Biohazard Sorting -- I. Recent Developments -- II. Introduction -- III. Critical Aspects of the Procedure -- IV. Applications and Future Directions -- Acknowledgment -- References -- Chapter 9: Guidelines for the Presentation of Flow Cytometric Data -- I. Introduction -- II. General Principles of Graphical Presentation -- III. Statistics -- IV. Subset Analysis -- V. Conclusion -- References -- Part II: Cellular DNA Content Analysis -- Chapter 10: Detergent and Proteolytic Enzyme-Based Techniques for Nuclear Isolation and DNA Content Analysis -- I. Introduction -- II. Basic Principles of the Methods -- III. Applications -- IV. Materials -- V. Methods -- VI. Results -- Acknowledgments -- References -- Chapter 11: DNA Content and Cell Cycle Analysis by the Propidium Iodide-Hypotonic Citrate Method -- I. Introduction -- II. Application -- III. Materials -- IV. Staining Procedure -- V. Caution -- VI. Instrument Setup -- VII. Comments -- VIII. Results -- References -- Chapter 12: DNA Analysis from Paraffin-Embedded Blocks -- I. Introduction -- II. Application -- III. Methods -- IV. Critical Aspects of Technique -- V. Alternative Methods for Sample Preparation -- Acknowledgment -- References -- Chapter 13: Flow Cytometry and Sorting of Plant Protoplasts and Cells -- I. Update -- II. Introduction -- III. Application -- IV. Materials -- V. Procedures -- VI. Critical Aspects of the Procedures -- VII. Controls and Standards -- VIII. Instruments -- IX. Results -- Acknowledgments -- References -- Chapter 14: DNA Content Histogram and Cell-Cycle Analysis -- I. Introduction. , II. DNA Content Histogram Basic Principles -- III. Cell-Cycle Analysis of DNA Content Histograms -- IV. Critical Aspects of DNA Content and Cell-Cycle Analysis -- V. Interpretation of DNA Content and Cell-Cycle Histograms -- VI. Conclusion -- References -- Chapter 15: Simultaneous Analysis of Cellular RNA and DNA Content -- I. Update -- II. Introduction -- III. Applications -- IV. Materials -- V Staining Procedures Employing AO -- VI. Staining RNA and DNA with PY and Hoechst 33342 -- VII. Critical Aspects of the Procedures -- VIII. Controls and Standards -- IX. Instruments -- X. Results -- XI Comparison of the Methods -- References -- Part III: Cell proliferation and the Death Assays -- Chapter 16: Immunochemical Quantitation of Bromodeoxyuridine: Application to Cell-Cycle Kinetics -- I. Update -- II. Introduction -- III. Applications -- IV. Materials -- V. Procedures -- VI. Critical Aspects of the Procedure -- VII. Controls and Standards -- VIII. Instruments -- IX. Results -- X. Summary -- References -- Chapter 17: Cell-Cycle Kinetics Estimated by Analysis of Bromodeoxyuridine Incorporation -- I. Introduction -- II. Applications -- III. Materials -- IV. Methods -- V. Critical Aspects of the Procedure -- References -- Chapter 18: Flow Cytometric Analysis of Cell Division History Using Dilution of Carboxyfluorescein Diacetate Succinimidyl Ester, a Stably . -- I. Introduction and Background -- II. Reagents and Solutions -- III. Preparation and Labeling of Cells -- IV. Gathering of Information Concurrent with Division -- V. Analysis of Data -- VI. Application of Carboxyfluorescein Diacetate Succinimidyl Ester to In Vitro Culture of Lymphocytes -- VII. Monitoring Lymphocyte Responses In Vivo -- VIII. Antigen Receptor Transgenic Models -- References. , Chapter 19: Antibodies Against the Ki-67 Protein: Assessment of the Growth Fraction and Tools for Cell Cycle Analysis -- I. Introduction -- II. Application -- III. Materials and Methods -- IV. Critical Aspects -- V. Controls and Standards -- VI. Examples of Results -- References -- Chapter 20: Detection of DNA Damage in Individual Cells by Analysis of Histone H2AX Phosphorylation -- I. Update -- II. Introduction -- III. Considerations in the Use of gammaH2AX as a Measure ofDouble-Stranded Breaks -- IV. Methods of Analysis -- V. Typical Results -- VI. Possible Applications -- VII. Conclusion -- References -- Chapter 21: Assays of Cell Viability -- I. Update -- II. Introduction -- III. Changes in Light Scatter During Cell Death -- IV. Cell Sensitivity to Trypsin and DNase -- V. Fluorescein Diacetate (FDA) Hydrolysis and PI Exclusion -- VI. Rh123 Uptake and PI Exclusion -- VII. PI Exclusion Followed by Counterstaining with Hoechst 33342 -- VIII. Hoechst 33342 Active Uptake and PI Exclusion -- IX. Controlled Extraction of Low MW DNA from Apoptotic Cells -- X. Sensitivity of DNA In Situ to Denaturation -- XI. Detection of DNA Strand Breaks in Apoptotic Cells -- XII. A Selective Procedure for DNA Extraction from Apoptotic Cells Applicable to Gel Electrophoresis and Flow Cytometry... -- XIII. Comparison of the Methods: Confirmation of the Apoptotic Mode of Cell Death -- References -- Chapter 22: Difficulties and Pitfalls in Analysis of Apoptosis -- I. Update -- II. Introduction -- III. AI may not be Correlated with Incidence of Cell Death -- IV. Difficulties in Estimating Frequency of Apoptosis by Analysis of DNA Fragmentation -- V. The Lack of Evidence is not Evidence for the Lack of Apoptosis -- VI. Misclassification of Apoptotic Bodies or Nuclear Fragments as Single Apoptotic Cells -- VII. Apoptosis Versus Necrosis Versus ``Necrotic Stage´´ of Apoptosis. , VIII. Selective Loss of Apoptotic Cells During Sample Preparation -- IX. Live Cells Engulfing Apoptotic Bodies Masquerade as Apoptotic Cells -- X The Problems with Commercial Kits and Reagents -- XI. Cell Morphology is still the Gold Standard for Identification of Apoptotic Cells -- XII. Laser Scanning Cytometry: Have your Cake and Eat it too -- References -- Part IV: Cell Surface Immunophenotyping -- Chapter 23: Cell Preparation for the Identification of Leukocytes -- I. Introduction -- II. Antibodies -- III. Tandem Fluorochromes -- IV. Cell Preparation and Staining Procedures -- V. Titering Antibodies -- VI. Solutions and Reagents -- References -- Chapter 24: Multicolor Immunophenotyping -- I. Update -- II. Introduction -- III. Methodology -- IV. Normal Immunophenotypic Patterns of Maturation -- V. Abnormal Immunophenotypic Patterns of Maturation -- References -- Chapter 25: Differential Diagnosis of T-Cell Lymphoproliferative Disorders by Flow Cytometry Immunophenotyping. Correlation with Morpholog. -- I. Introduction -- II. Materials -- III. Methods -- IV. Identification of Abnormal T-Cell Population by Flow Cytometry -- V. Precursor T-Lymphoblastic Lymphoma/Leukemia (T-ALL) -- VI. Peripheral (Mature/Postthymic) Lymphoma Versus T-ALL -- VII. Thymocytes from Thymic Hyperplasia/Thymoma Versus T-ALL -- VIII. Mature T-Cell Lymphoproliferative Disorders -- IX. Conclusions -- References -- Chapter 26: B Cell Immunophenotyping -- I. Update -- II. Introduction -- III. Immunophenotyping of B Cell Developmental Stages in the Bone Marrow -- IV. Peripheral B Cell Populations -- V. Summary -- VI. Antigen-Induced B Cell Subsets -- VII. Antigen-Specific B Cells -- References -- Part V: Cytogenetics/Chromatin Structure -- Chapter 27: Telomere Length Measurements Using Fluorescence In Situ Hybridization and Flow Cytometry -- I. Introduction -- II. Background. , III. Methods.

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